VASt domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

GRAM domain containing protein, putative

Species:

Leishmania major

UniProt:

Q4QEC5_LEIMA

TriTrypDb:

LmjF.17.0730 , LMJLV39_170013100 , LMJSD75_170012700

Length:

658

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 8
NetGPI	no	yes: 0, no: 8

Cellular components
Term	Name	Level	Count
GO:0005789	endoplasmic reticulum membrane	4	2
GO:0005886	plasma membrane	3	2
GO:0016020	membrane	2	9
GO:0031090	organelle membrane	3	2
GO:0044232	organelle membrane contact site	2	2
GO:0110165	cellular anatomical entity	1	9
GO:0140268	endoplasmic reticulum-plasma membrane contact site	3	2

Expansion

Sequence features

Q4QEC5

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4QEC5

Function

Biological processes
Term	Name	Level	Count
GO:0006810	transport	3	2
GO:0006869	lipid transport	5	2
GO:0009987	cellular process	1	2
GO:0015850	organic hydroxy compound transport	5	2
GO:0015918	sterol transport	6	2
GO:0032365	intracellular lipid transport	4	2
GO:0032366	intracellular sterol transport	5	2
GO:0046907	intracellular transport	3	2
GO:0051179	localization	1	2
GO:0051234	establishment of localization	2	2
GO:0051641	cellular localization	2	2
GO:0051649	establishment of localization in cell	3	2
GO:0071702	organic substance transport	4	2

Molecular functions
Term	Name	Level	Count
GO:0005215	transporter activity	1	2
GO:0005319	lipid transporter activity	2	2
GO:0005488	binding	1	2
GO:0005496	steroid binding	3	2
GO:0008289	lipid binding	2	2
GO:0015248	sterol transporter activity	3	2
GO:0032934	sterol binding	4	2
GO:0097159	organic cyclic compound binding	2	2
GO:0120013	lipid transfer activity	3	2
GO:0120015	sterol transfer activity	4	2

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	118	122	PF00656	0.572
CLV_C14_Caspase3-7	217	221	PF00656	0.342
CLV_C14_Caspase3-7	525	529	PF00656	0.385
CLV_NRD_NRD_1	185	187	PF00675	0.772
CLV_NRD_NRD_1	206	208	PF00675	0.598
CLV_NRD_NRD_1	30	32	PF00675	0.601
CLV_NRD_NRD_1	402	404	PF00675	0.473
CLV_NRD_NRD_1	559	561	PF00675	0.777
CLV_PCSK_KEX2_1	206	208	PF00082	0.598
CLV_PCSK_SKI1_1	170	174	PF00082	0.725
CLV_PCSK_SKI1_1	180	184	PF00082	0.687
CLV_PCSK_SKI1_1	198	202	PF00082	0.706
CLV_PCSK_SKI1_1	206	210	PF00082	0.553
CLV_PCSK_SKI1_1	269	273	PF00082	0.565
CLV_PCSK_SKI1_1	379	383	PF00082	0.509
CLV_PCSK_SKI1_1	426	430	PF00082	0.467
CLV_PCSK_SKI1_1	449	453	PF00082	0.467
DEG_APCC_DBOX_1	448	456	PF00400	0.342
DEG_Nend_Nbox_1	1	3	PF02207	0.340
DOC_CDC14_PxL_1	455	463	PF14671	0.217
DOC_CKS1_1	162	167	PF01111	0.509
DOC_CKS1_1	2	7	PF01111	0.401
DOC_CKS1_1	369	374	PF01111	0.407
DOC_CYCLIN_yCln2_LP_2	369	375	PF00134	0.292
DOC_CYCLIN_yCln2_LP_2	631	637	PF00134	0.542
DOC_MAPK_DCC_7	367	375	PF00069	0.312
DOC_MAPK_DCC_7	454	463	PF00069	0.217
DOC_MAPK_gen_1	263	273	PF00069	0.342
DOC_MAPK_gen_1	292	300	PF00069	0.217
DOC_MAPK_MEF2A_6	454	463	PF00069	0.306
DOC_MAPK_MEF2A_6	575	582	PF00069	0.457
DOC_PP1_RVXF_1	5	11	PF00149	0.340
DOC_PP4_FxxP_1	245	248	PF00568	0.342
DOC_USP7_MATH_1	103	107	PF00917	0.518
DOC_USP7_MATH_1	124	128	PF00917	0.487
DOC_USP7_MATH_1	191	195	PF00917	0.570
DOC_USP7_MATH_1	341	345	PF00917	0.374
DOC_USP7_MATH_1	527	531	PF00917	0.266
DOC_USP7_MATH_1	554	558	PF00917	0.538
DOC_USP7_MATH_1	567	571	PF00917	0.593
DOC_USP7_MATH_1	93	97	PF00917	0.516
DOC_USP7_UBL2_3	309	313	PF12436	0.419
DOC_WW_Pin1_4	1	6	PF00397	0.422
DOC_WW_Pin1_4	128	133	PF00397	0.564
DOC_WW_Pin1_4	161	166	PF00397	0.614
DOC_WW_Pin1_4	244	249	PF00397	0.342
DOC_WW_Pin1_4	368	373	PF00397	0.427
DOC_WW_Pin1_4	568	573	PF00397	0.499
DOC_WW_Pin1_4	630	635	PF00397	0.655
DOC_WW_Pin1_4	99	104	PF00397	0.488
LIG_14-3-3_CanoR_1	152	158	PF00244	0.551
LIG_14-3-3_CanoR_1	31	36	PF00244	0.402
LIG_14-3-3_CanoR_1	426	434	PF00244	0.308
LIG_14-3-3_CanoR_1	516	522	PF00244	0.328
LIG_14-3-3_CanoR_1	7	15	PF00244	0.405
LIG_14-3-3_CanoR_1	81	86	PF00244	0.459
LIG_Actin_WH2_2	266	283	PF00022	0.342
LIG_AP2alpha_1	474	478	PF02296	0.292
LIG_BRCT_BRCA1_1	284	288	PF00533	0.379
LIG_BRCT_BRCA1_1	606	610	PF00533	0.394
LIG_Clathr_ClatBox_1	270	274	PF01394	0.342
LIG_deltaCOP1_diTrp_1	407	413	PF00928	0.318
LIG_EH1_1	477	485	PF00400	0.259
LIG_eIF4E_1	447	453	PF01652	0.342
LIG_FHA_1	152	158	PF00498	0.550
LIG_FHA_1	171	177	PF00498	0.522
LIG_FHA_1	213	219	PF00498	0.398
LIG_FHA_1	31	37	PF00498	0.380
LIG_FHA_1	391	397	PF00498	0.342
LIG_FHA_1	429	435	PF00498	0.267
LIG_FHA_1	458	464	PF00498	0.266
LIG_FHA_1	51	57	PF00498	0.299
LIG_FHA_1	630	636	PF00498	0.651
LIG_FHA_1	638	644	PF00498	0.337
LIG_FHA_1	651	657	PF00498	0.324
LIG_FHA_2	146	152	PF00498	0.549
LIG_FHA_2	199	205	PF00498	0.481
LIG_FHA_2	215	221	PF00498	0.386
LIG_FHA_2	328	334	PF00498	0.483
LIG_FHA_2	441	447	PF00498	0.231
LIG_GBD_Chelix_1	533	541	PF00786	0.553
LIG_LIR_Apic_2	83	89	PF02991	0.451
LIG_LIR_Gen_1	239	250	PF02991	0.267
LIG_LIR_Gen_1	255	264	PF02991	0.267
LIG_LIR_Gen_1	274	284	PF02991	0.200
LIG_LIR_Gen_1	285	296	PF02991	0.258
LIG_LIR_Gen_1	346	355	PF02991	0.328
LIG_LIR_Gen_1	360	370	PF02991	0.303
LIG_LIR_Gen_1	49	60	PF02991	0.468
LIG_LIR_Gen_1	61	70	PF02991	0.293
LIG_LIR_Gen_1	653	658	PF02991	0.545
LIG_LIR_Nem_3	204	208	PF02991	0.301
LIG_LIR_Nem_3	239	245	PF02991	0.267
LIG_LIR_Nem_3	255	261	PF02991	0.267
LIG_LIR_Nem_3	274	280	PF02991	0.193
LIG_LIR_Nem_3	285	291	PF02991	0.223
LIG_LIR_Nem_3	315	321	PF02991	0.516
LIG_LIR_Nem_3	346	351	PF02991	0.350
LIG_LIR_Nem_3	360	365	PF02991	0.332
LIG_LIR_Nem_3	443	447	PF02991	0.266
LIG_LIR_Nem_3	49	55	PF02991	0.360
LIG_LIR_Nem_3	607	612	PF02991	0.348
LIG_LIR_Nem_3	61	67	PF02991	0.323
LIG_LIR_Nem_3	613	619	PF02991	0.498
LIG_LIR_Nem_3	653	658	PF02991	0.545
LIG_MLH1_MIPbox_1	606	610	PF16413	0.394
LIG_PDZ_Class_2	653	658	PF00595	0.605
LIG_Pex14_2	10	14	PF04695	0.365
LIG_Pex14_2	205	209	PF04695	0.342
LIG_Pex14_2	385	389	PF04695	0.267
LIG_Pex14_2	474	478	PF04695	0.292
LIG_SH2_CRK	318	322	PF00017	0.402
LIG_SH2_CRK	64	68	PF00017	0.396
LIG_SH2_PTP2	2	5	PF00017	0.401
LIG_SH2_PTP2	277	280	PF00017	0.342
LIG_SH2_PTP2	577	580	PF00017	0.497
LIG_SH2_SRC	577	580	PF00017	0.455
LIG_SH2_STAT5	2	5	PF00017	0.401
LIG_SH2_STAT5	277	280	PF00017	0.342
LIG_SH2_STAT5	442	445	PF00017	0.330
LIG_SH2_STAT5	504	507	PF00017	0.333
LIG_SH2_STAT5	577	580	PF00017	0.497
LIG_SH3_1	207	213	PF00018	0.342
LIG_SH3_1	409	415	PF00018	0.275
LIG_SH3_2	412	417	PF14604	0.301
LIG_SH3_3	126	132	PF00018	0.503
LIG_SH3_3	159	165	PF00018	0.550
LIG_SH3_3	197	203	PF00018	0.478
LIG_SH3_3	207	213	PF00018	0.282
LIG_SH3_3	318	324	PF00018	0.535
LIG_SH3_3	409	415	PF00018	0.275
LIG_SH3_3	563	569	PF00018	0.580
LIG_SH3_3	573	579	PF00018	0.481
LIG_SUMO_SIM_anti_2	16	24	PF11976	0.356
LIG_SUMO_SIM_anti_2	38	44	PF11976	0.379
LIG_SUMO_SIM_par_1	16	24	PF11976	0.409
LIG_SUMO_SIM_par_1	198	204	PF11976	0.432
LIG_SUMO_SIM_par_1	25	30	PF11976	0.355
LIG_SUMO_SIM_par_1	269	274	PF11976	0.292
LIG_SUMO_SIM_par_1	38	44	PF11976	0.376
LIG_SUMO_SIM_par_1	595	600	PF11976	0.415
LIG_WRC_WIRS_1	483	488	PF05994	0.342
LIG_WRC_WIRS_1	619	624	PF05994	0.584
LIG_WRC_WIRS_1	645	650	PF05994	0.457
MOD_CDK_SPxK_1	1	7	PF00069	0.400
MOD_CDK_SPxK_1	368	374	PF00069	0.386
MOD_CDK_SPxxK_3	568	575	PF00069	0.508
MOD_CK1_1	101	107	PF00069	0.574
MOD_CK1_1	174	180	PF00069	0.509
MOD_CK1_1	282	288	PF00069	0.290
MOD_CK1_1	319	325	PF00069	0.490
MOD_CK1_1	357	363	PF00069	0.341
MOD_CK1_1	491	497	PF00069	0.342
MOD_CK1_1	630	636	PF00069	0.695
MOD_CK1_1	91	97	PF00069	0.540
MOD_CK2_1	10	16	PF00069	0.420
MOD_CK2_1	198	204	PF00069	0.500
MOD_CK2_1	249	255	PF00069	0.365
MOD_CK2_1	327	333	PF00069	0.429
MOD_CK2_1	346	352	PF00069	0.354
MOD_CK2_1	440	446	PF00069	0.223
MOD_CK2_1	567	573	PF00069	0.603
MOD_CK2_1	69	75	PF00069	0.407
MOD_CMANNOS	410	413	PF00535	0.467
MOD_GlcNHglycan	109	112	PF01048	0.736
MOD_GlcNHglycan	157	160	PF01048	0.798
MOD_GlcNHglycan	173	176	PF01048	0.701
MOD_GlcNHglycan	179	183	PF01048	0.745
MOD_GlcNHglycan	281	284	PF01048	0.544
MOD_GlcNHglycan	302	305	PF01048	0.470
MOD_GlcNHglycan	317	321	PF01048	0.719
MOD_GlcNHglycan	356	359	PF01048	0.646
MOD_GlcNHglycan	455	458	PF01048	0.516
MOD_GlcNHglycan	490	493	PF01048	0.533
MOD_GlcNHglycan	545	548	PF01048	0.719
MOD_GlcNHglycan	599	602	PF01048	0.415
MOD_GlcNHglycan	95	98	PF01048	0.754
MOD_GSK3_1	124	131	PF00069	0.572
MOD_GSK3_1	141	148	PF00069	0.530
MOD_GSK3_1	151	158	PF00069	0.493
MOD_GSK3_1	170	177	PF00069	0.498
MOD_GSK3_1	208	215	PF00069	0.370
MOD_GSK3_1	282	289	PF00069	0.279
MOD_GSK3_1	312	319	PF00069	0.473
MOD_GSK3_1	346	353	PF00069	0.340
MOD_GSK3_1	453	460	PF00069	0.342
MOD_GSK3_1	484	491	PF00069	0.332
MOD_GSK3_1	50	57	PF00069	0.386
MOD_GSK3_1	517	524	PF00069	0.382
MOD_GSK3_1	629	636	PF00069	0.705
MOD_GSK3_1	88	95	PF00069	0.513
MOD_GSK3_1	98	105	PF00069	0.532
MOD_N-GLC_1	218	223	PF02516	0.418
MOD_N-GLC_1	592	597	PF02516	0.415
MOD_NEK2_1	10	15	PF00069	0.410
MOD_NEK2_1	157	162	PF00069	0.611
MOD_NEK2_1	228	233	PF00069	0.297
MOD_NEK2_1	236	241	PF00069	0.328
MOD_NEK2_1	249	254	PF00069	0.248
MOD_NEK2_1	286	291	PF00069	0.356
MOD_NEK2_1	305	310	PF00069	0.366
MOD_NEK2_1	327	332	PF00069	0.448
MOD_NEK2_1	428	433	PF00069	0.267
MOD_NEK2_1	453	458	PF00069	0.342
MOD_NEK2_1	507	512	PF00069	0.301
MOD_NEK2_1	55	60	PF00069	0.385
MOD_NEK2_1	597	602	PF00069	0.427
MOD_NEK2_1	604	609	PF00069	0.403
MOD_NEK2_1	610	615	PF00069	0.378
MOD_NEK2_1	66	71	PF00069	0.318
MOD_NEK2_2	495	500	PF00069	0.306
MOD_PIKK_1	124	130	PF00454	0.546
MOD_PIKK_1	174	180	PF00454	0.593
MOD_PIKK_1	236	242	PF00454	0.342
MOD_PIKK_1	346	352	PF00454	0.406
MOD_PIKK_1	464	470	PF00454	0.342
MOD_PIKK_1	484	490	PF00454	0.267
MOD_PIKK_1	58	64	PF00454	0.387
MOD_PIKK_1	620	626	PF00454	0.624
MOD_PKA_1	31	37	PF00069	0.412
MOD_PKA_1	403	409	PF00069	0.274
MOD_PKA_2	106	112	PF00069	0.540
MOD_PKA_2	151	157	PF00069	0.540
MOD_PKA_2	30	36	PF00069	0.384
MOD_PKA_2	413	419	PF00069	0.351
MOD_PKA_2	80	86	PF00069	0.371
MOD_Plk_1	178	184	PF00069	0.556
MOD_Plk_1	218	224	PF00069	0.217
MOD_Plk_1	360	366	PF00069	0.352
MOD_Plk_1	495	501	PF00069	0.342
MOD_Plk_1	50	56	PF00069	0.421
MOD_Plk_1	592	598	PF00069	0.415
MOD_Plk_2-3	145	151	PF00069	0.560
MOD_Plk_4	10	16	PF00069	0.433
MOD_Plk_4	31	37	PF00069	0.387
MOD_Plk_4	429	435	PF00069	0.292
MOD_Plk_4	440	446	PF00069	0.217
MOD_Plk_4	507	513	PF00069	0.301
MOD_Plk_4	517	523	PF00069	0.318
MOD_Plk_4	592	598	PF00069	0.402
MOD_Plk_4	604	610	PF00069	0.408
MOD_Plk_4	612	618	PF00069	0.492
MOD_Plk_4	66	72	PF00069	0.346
MOD_Plk_4	81	87	PF00069	0.417
MOD_ProDKin_1	1	7	PF00069	0.421
MOD_ProDKin_1	128	134	PF00069	0.565
MOD_ProDKin_1	161	167	PF00069	0.614
MOD_ProDKin_1	244	250	PF00069	0.342
MOD_ProDKin_1	368	374	PF00069	0.431
MOD_ProDKin_1	568	574	PF00069	0.498
MOD_ProDKin_1	630	636	PF00069	0.651
MOD_ProDKin_1	99	105	PF00069	0.489
MOD_SUMO_rev_2	24	34	PF00179	0.401
MOD_SUMO_rev_2	262	271	PF00179	0.274
MOD_SUMO_rev_2	332	340	PF00179	0.428
MOD_SUMO_rev_2	397	406	PF00179	0.292
MOD_SUMO_rev_2	570	576	PF00179	0.483
TRG_DiLeu_BaEn_3	16	22	PF01217	0.402
TRG_ENDOCYTIC_2	277	280	PF00928	0.342
TRG_ENDOCYTIC_2	318	321	PF00928	0.403
TRG_ENDOCYTIC_2	442	445	PF00928	0.299
TRG_ENDOCYTIC_2	450	453	PF00928	0.238
TRG_ENDOCYTIC_2	504	507	PF00928	0.333
TRG_ENDOCYTIC_2	577	580	PF00928	0.497
TRG_ENDOCYTIC_2	64	67	PF00928	0.393
TRG_ER_diArg_1	205	207	PF00400	0.395

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A1X0NSV7	Trypanosomatidae	39%	92%
A0A3R7LZ26	Trypanosoma rangeli	40%	100%
A0A3S7WUF7	Leishmania donovani	93%	100%
A4H911	Leishmania braziliensis	79%	92%
A4HXE3	Leishmania infantum	93%	100%
E9AR38	Leishmania mexicana (strain MHOM/GT/2001/U1103)	89%	100%
V5B9G2	Trypanosoma cruzi	38%	92%