3-oxo-5-alpha-steroid 4-dehydrogenase-like protein

Quick info Annotations Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

3-oxo-5-alpha-steroid 4-dehydrogenase-like protein

Species:

Leishmania major

UniProt:

Q4QE81_LEIMA

TriTrypDb:

LmjF.17.1100 , LMJLV39_170018300 , LMJSD75_170018000

Length:

543

Annotations

LeishMANIAdb annotations

Related to other eukaryotic steroid 5 alpha reductases. Localization: ER (by homology)

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 6
NetGPI	no	yes: 0, no: 6

Cellular components
Term	Name	Level	Count
GO:0016020	membrane	2	7
GO:0110165	cellular anatomical entity	1	7

Expansion

Sequence features

Q4QE81

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4QE81

Function

Biological processes
Term	Name	Level	Count
GO:0006629	lipid metabolic process	3	7
GO:0008152	metabolic process	1	7
GO:0044238	primary metabolic process	2	7
GO:0071704	organic substance metabolic process	2	7

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	7
GO:0003865	3-oxo-5-alpha-steroid 4-dehydrogenase activity	5	6
GO:0016229	steroid dehydrogenase activity	3	6
GO:0016491	oxidoreductase activity	2	7
GO:0016627	oxidoreductase activity, acting on the CH-CH group of donors	3	7
GO:0033765	steroid dehydrogenase activity, acting on the CH-CH group of donors	4	6

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	404	408	PF00656	0.574
CLV_C14_Caspase3-7	84	88	PF00656	0.682
CLV_MEL_PAP_1	135	141	PF00089	0.576
CLV_NRD_NRD_1	361	363	PF00675	0.437
CLV_NRD_NRD_1	378	380	PF00675	0.496
CLV_PCSK_FUR_1	359	363	PF00082	0.445
CLV_PCSK_KEX2_1	361	363	PF00082	0.438
CLV_PCSK_KEX2_1	378	380	PF00082	0.492
CLV_PCSK_SKI1_1	300	304	PF00082	0.545
CLV_PCSK_SKI1_1	485	489	PF00082	0.450
CLV_PCSK_SKI1_1	535	539	PF00082	0.346
CLV_Separin_Metazoa	460	464	PF03568	0.590
DEG_APCC_DBOX_1	225	233	PF00400	0.403
DEG_APCC_DBOX_1	299	307	PF00400	0.478
DEG_SPOP_SBC_1	175	179	PF00917	0.533
DEG_SPOP_SBC_1	389	393	PF00917	0.793
DEG_SPOP_SBC_1	464	468	PF00917	0.671
DOC_ANK_TNKS_1	402	409	PF00023	0.486
DOC_CDC14_PxL_1	157	165	PF14671	0.437
DOC_CYCLIN_yCln2_LP_2	232	238	PF00134	0.471
DOC_CYCLIN_yCln2_LP_2	60	66	PF00134	0.574
DOC_MAPK_DCC_7	226	234	PF00069	0.365
DOC_MAPK_MEF2A_6	322	331	PF00069	0.409
DOC_PP1_RVXF_1	136	142	PF00149	0.422
DOC_PP1_RVXF_1	320	326	PF00149	0.345
DOC_PP1_RVXF_1	483	489	PF00149	0.450
DOC_PP1_RVXF_1	92	98	PF00149	0.635
DOC_PP2B_LxvP_1	133	136	PF13499	0.477
DOC_PP2B_LxvP_1	232	235	PF13499	0.385
DOC_PP2B_LxvP_1	60	63	PF13499	0.580
DOC_PP4_FxxP_1	64	67	PF00568	0.687
DOC_SPAK_OSR1_1	115	119	PF12202	0.512
DOC_USP7_MATH_1	175	179	PF00917	0.542
DOC_USP7_MATH_1	277	281	PF00917	0.539
DOC_USP7_MATH_1	302	306	PF00917	0.437
DOC_USP7_MATH_1	312	316	PF00917	0.393
DOC_USP7_MATH_1	387	391	PF00917	0.747
DOC_USP7_MATH_1	440	444	PF00917	0.615
DOC_USP7_MATH_1	464	468	PF00917	0.671
DOC_USP7_MATH_1	90	94	PF00917	0.641
DOC_WW_Pin1_4	143	148	PF00397	0.325
DOC_WW_Pin1_4	164	169	PF00397	0.477
DOC_WW_Pin1_4	281	286	PF00397	0.555
DOC_WW_Pin1_4	305	310	PF00397	0.437
LIG_14-3-3_CanoR_1	115	121	PF00244	0.411
LIG_14-3-3_CanoR_1	124	130	PF00244	0.463
LIG_14-3-3_CanoR_1	138	142	PF00244	0.327
LIG_14-3-3_CanoR_1	206	216	PF00244	0.428
LIG_14-3-3_CanoR_1	279	287	PF00244	0.457
LIG_14-3-3_CanoR_1	403	407	PF00244	0.456
LIG_14-3-3_CanoR_1	463	473	PF00244	0.647
LIG_14-3-3_CanoR_1	81	89	PF00244	0.709
LIG_14-3-3_CanoR_1	98	106	PF00244	0.475
LIG_Actin_WH2_2	8	25	PF00022	0.450
LIG_Actin_WH2_2	99	117	PF00022	0.512
LIG_APCC_ABBA_1	32	37	PF00400	0.673
LIG_APCC_ABBA_1	381	386	PF00400	0.627
LIG_BIR_II_1	1	5	PF00653	0.459
LIG_BRCT_BRCA1_1	127	131	PF00533	0.433
LIG_BRCT_BRCA1_1	339	343	PF00533	0.512
LIG_BRCT_BRCA1_1	98	102	PF00533	0.546
LIG_CSL_BTD_1	60	63	PF09270	0.580
LIG_deltaCOP1_diTrp_1	57	64	PF00928	0.751
LIG_FHA_1	103	109	PF00498	0.431
LIG_FHA_1	16	22	PF00498	0.459
LIG_FHA_1	431	437	PF00498	0.618
LIG_FHA_1	452	458	PF00498	0.601
LIG_FHA_2	235	241	PF00498	0.429
LIG_FHA_2	453	459	PF00498	0.671
LIG_FHA_2	523	529	PF00498	0.618
LIG_Integrin_isoDGR_2	256	258	PF01839	0.564
LIG_Integrin_RGD_1	362	364	PF01839	0.469
LIG_LIR_Apic_2	227	231	PF02991	0.375
LIG_LIR_Apic_2	407	412	PF02991	0.574
LIG_LIR_Gen_1	110	118	PF02991	0.461
LIG_LIR_Gen_1	148	158	PF02991	0.416
LIG_LIR_Gen_1	18	26	PF02991	0.465
LIG_LIR_Gen_1	250	259	PF02991	0.372
LIG_LIR_Gen_1	433	441	PF02991	0.576
LIG_LIR_Gen_1	468	478	PF02991	0.618
LIG_LIR_Gen_1	528	538	PF02991	0.618
LIG_LIR_LC3C_4	240	245	PF02991	0.424
LIG_LIR_Nem_3	110	114	PF02991	0.461
LIG_LIR_Nem_3	148	153	PF02991	0.383
LIG_LIR_Nem_3	159	163	PF02991	0.430
LIG_LIR_Nem_3	18	22	PF02991	0.465
LIG_LIR_Nem_3	200	205	PF02991	0.408
LIG_LIR_Nem_3	250	255	PF02991	0.378
LIG_LIR_Nem_3	354	358	PF02991	0.580
LIG_LIR_Nem_3	431	435	PF02991	0.493
LIG_LIR_Nem_3	467	473	PF02991	0.705
LIG_LIR_Nem_3	502	506	PF02991	0.411
LIG_LIR_Nem_3	525	529	PF02991	0.543
LIG_LIR_Nem_3	59	64	PF02991	0.705
LIG_LYPXL_yS_3	160	163	PF13949	0.482
LIG_MYND_1	171	175	PF01753	0.531
LIG_MYND_1	63	67	PF01753	0.690
LIG_NRBOX	213	219	PF00104	0.449
LIG_PCNA_PIPBox_1	248	257	PF02747	0.419
LIG_Pex14_1	127	131	PF04695	0.492
LIG_SH2_CRK	111	115	PF00017	0.450
LIG_SH2_CRK	409	413	PF00017	0.546
LIG_SH2_CRK	530	534	PF00017	0.618
LIG_SH2_PTP2	435	438	PF00017	0.546
LIG_SH2_SRC	435	438	PF00017	0.618
LIG_SH2_STAP1	287	291	PF00017	0.454
LIG_SH2_STAP1	522	526	PF00017	0.618
LIG_SH2_STAT3	72	75	PF00017	0.648
LIG_SH2_STAT5	223	226	PF00017	0.363
LIG_SH2_STAT5	24	27	PF00017	0.644
LIG_SH2_STAT5	252	255	PF00017	0.374
LIG_SH2_STAT5	287	290	PF00017	0.499
LIG_SH2_STAT5	33	36	PF00017	0.643
LIG_SH2_STAT5	356	359	PF00017	0.589
LIG_SH2_STAT5	435	438	PF00017	0.546
LIG_SH2_STAT5	506	509	PF00017	0.411
LIG_SH2_STAT5	530	533	PF00017	0.618
LIG_SH2_STAT5	72	75	PF00017	0.577
LIG_SH3_3	115	121	PF00018	0.411
LIG_SH3_3	165	171	PF00018	0.479
LIG_SUMO_SIM_par_1	104	110	PF11976	0.512
LIG_SUMO_SIM_par_1	240	246	PF11976	0.391
LIG_TRAF2_1	456	459	PF00917	0.593
LIG_TRAF2_1	67	70	PF00917	0.736
LIG_TYR_ITIM	158	163	PF00017	0.579
LIG_TYR_ITSM	431	438	PF00017	0.512
MOD_CK1_1	137	143	PF00069	0.469
MOD_CK1_1	169	175	PF00069	0.637
MOD_CK1_1	178	184	PF00069	0.661
MOD_CK1_1	190	196	PF00069	0.520
MOD_CK1_1	281	287	PF00069	0.584
MOD_CK1_1	305	311	PF00069	0.628
MOD_CK1_1	390	396	PF00069	0.585
MOD_CK1_1	443	449	PF00069	0.599
MOD_CK1_1	452	458	PF00069	0.434
MOD_CK1_1	466	472	PF00069	0.396
MOD_CK1_1	49	55	PF00069	0.562
MOD_CK2_1	389	395	PF00069	0.651
MOD_CK2_1	452	458	PF00069	0.550
MOD_CK2_1	486	492	PF00069	0.486
MOD_CMANNOS	58	61	PF00535	0.667
MOD_Cter_Amidation	376	379	PF01082	0.736
MOD_GlcNHglycan	1	4	PF01048	0.577
MOD_GlcNHglycan	178	181	PF01048	0.707
MOD_GlcNHglycan	193	196	PF01048	0.403
MOD_GlcNHglycan	263	266	PF01048	0.573
MOD_GlcNHglycan	27	30	PF01048	0.652
MOD_GlcNHglycan	280	283	PF01048	0.638
MOD_GlcNHglycan	310	313	PF01048	0.685
MOD_GlcNHglycan	339	342	PF01048	0.512
MOD_GlcNHglycan	40	43	PF01048	0.478
MOD_GlcNHglycan	442	445	PF01048	0.555
MOD_GlcNHglycan	488	491	PF01048	0.513
MOD_GlcNHglycan	83	86	PF01048	0.661
MOD_GlcNHglycan	99	102	PF01048	0.249
MOD_GSK3_1	174	181	PF00069	0.684
MOD_GSK3_1	187	194	PF00069	0.570
MOD_GSK3_1	230	237	PF00069	0.488
MOD_GSK3_1	243	250	PF00069	0.448
MOD_GSK3_1	273	280	PF00069	0.695
MOD_GSK3_1	281	288	PF00069	0.641
MOD_GSK3_1	304	311	PF00069	0.618
MOD_GSK3_1	387	394	PF00069	0.732
MOD_GSK3_1	439	446	PF00069	0.443
MOD_GSK3_1	465	472	PF00069	0.645
MOD_GSK3_1	486	493	PF00069	0.614
MOD_N-GLC_1	446	451	PF02516	0.510
MOD_NEK2_1	102	107	PF00069	0.460
MOD_NEK2_1	116	121	PF00069	0.600
MOD_NEK2_1	131	136	PF00069	0.610
MOD_NEK2_1	15	20	PF00069	0.507
MOD_NEK2_1	243	248	PF00069	0.482
MOD_NEK2_1	337	342	PF00069	0.482
MOD_NEK2_1	388	393	PF00069	0.723
MOD_NEK2_1	451	456	PF00069	0.513
MOD_NEK2_1	465	470	PF00069	0.539
MOD_NEK2_2	247	252	PF00069	0.469
MOD_PIKK_1	166	172	PF00454	0.447
MOD_PIKK_1	207	213	PF00454	0.494
MOD_PIKK_1	65	71	PF00454	0.591
MOD_PKA_2	123	129	PF00069	0.586
MOD_PKA_2	137	143	PF00069	0.385
MOD_PKA_2	278	284	PF00069	0.584
MOD_PKA_2	402	408	PF00069	0.409
MOD_PKA_2	80	86	PF00069	0.645
MOD_PKA_2	97	103	PF00069	0.336
MOD_Plk_4	137	143	PF00069	0.469
MOD_Plk_4	145	151	PF00069	0.448
MOD_Plk_4	15	21	PF00069	0.512
MOD_Plk_4	169	175	PF00069	0.659
MOD_Plk_4	197	203	PF00069	0.525
MOD_Plk_4	247	253	PF00069	0.453
MOD_Plk_4	466	472	PF00069	0.573
MOD_ProDKin_1	143	149	PF00069	0.380
MOD_ProDKin_1	164	170	PF00069	0.603
MOD_ProDKin_1	281	287	PF00069	0.698
MOD_ProDKin_1	305	311	PF00069	0.535
TRG_DiLeu_BaEn_1	159	164	PF01217	0.564
TRG_ENDOCYTIC_2	111	114	PF00928	0.512
TRG_ENDOCYTIC_2	154	157	PF00928	0.575
TRG_ENDOCYTIC_2	160	163	PF00928	0.502
TRG_ENDOCYTIC_2	252	255	PF00928	0.450
TRG_ENDOCYTIC_2	355	358	PF00928	0.465
TRG_ENDOCYTIC_2	435	438	PF00928	0.411
TRG_ENDOCYTIC_2	530	533	PF00928	0.411
TRG_ER_diArg_1	358	361	PF00400	0.510
TRG_Pf-PMV_PEXEL_1	23	27	PF00026	0.589

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P837	Leptomonas seymouri	46%	100%
A0A3S7WUK3	Leishmania donovani	91%	100%
A4H965	Leishmania braziliensis	72%	100%
A4HXI7	Leishmania infantum	92%	100%
E9AR82	Leishmania mexicana (strain MHOM/GT/2001/U1103)	85%	100%