Cytochrome b5 heme-binding domain-containing protein

Quick info Annotations Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

cytochrome b5-like Heme/Steroid binding domain containing protein, putative

Species:

Leishmania major

UniProt:

Q4QE55_LEIMA

TriTrypDb:

LmjF.17.1350 , LMJLV39_170020900 , LMJSD75_170020600

Length:

179

Annotations

LeishMANIAdb annotations

This cluster contains a high variety of cytochrome b5 homologs. Although all membrane-anchored, some use the N-terminal [type-III] while others the C-terminal TM helix [type-IV]. The heme domain is assumed to be cytoplasmic.. Localization: ER (by homology) / Mitochondrial outer membrane (by homology)

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 6
NetGPI	no	yes: 0, no: 6

Cellular components
Term	Name	Level	Count
GO:0016020	membrane	2	4
GO:0043226	organelle	2	2
GO:0043227	membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043231	intracellular membrane-bounded organelle	4	2
GO:0110165	cellular anatomical entity	1	4

Expansion

Sequence features

Q4QE55

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4QE55

Function

Could not find GO biological_process term for this entry.

Molecular functions
Term	Name	Level	Count
GO:0005488	binding	1	5
GO:0020037	heme binding	4	5
GO:0043167	ion binding	2	5
GO:0043169	cation binding	3	5
GO:0046872	metal ion binding	4	5
GO:0046906	tetrapyrrole binding	3	5
GO:0097159	organic cyclic compound binding	2	5
GO:1901363	heterocyclic compound binding	2	5

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	44	48	PF00656	0.677
CLV_PCSK_KEX2_1	37	39	PF00082	0.380
CLV_PCSK_PC1ET2_1	37	39	PF00082	0.380
CLV_PCSK_SKI1_1	175	179	PF00082	0.362
DEG_COP1_1	43	53	PF00400	0.706
DEG_SPOP_SBC_1	55	59	PF00917	0.655
DOC_MAPK_gen_1	112	121	PF00069	0.472
DOC_MAPK_MEF2A_6	114	123	PF00069	0.508
DOC_PP4_FxxP_1	159	162	PF00568	0.503
DOC_USP7_MATH_1	55	59	PF00917	0.710
DOC_WW_Pin1_4	63	68	PF00397	0.735
LIG_14-3-3_CanoR_1	104	110	PF00244	0.502
LIG_14-3-3_CanoR_1	166	172	PF00244	0.472
LIG_EH1_1	170	178	PF00400	0.490
LIG_FHA_1	162	168	PF00498	0.471
LIG_FHA_1	64	70	PF00498	0.753
LIG_FHA_2	104	110	PF00498	0.466
LIG_FHA_2	113	119	PF00498	0.466
LIG_FHA_2	83	89	PF00498	0.482
LIG_KLC1_Yacidic_2	84	88	PF13176	0.476
LIG_LIR_Gen_1	117	128	PF02991	0.466
LIG_LIR_Gen_1	154	162	PF02991	0.475
LIG_LIR_Nem_3	117	123	PF02991	0.466
LIG_LIR_Nem_3	154	159	PF02991	0.475
LIG_PCNA_PIPBox_1	152	161	PF02747	0.508
LIG_PCNA_PIPBox_1	68	77	PF02747	0.585
LIG_SH2_STAT5	158	161	PF00017	0.472
LIG_SH2_STAT5	86	89	PF00017	0.670
LIG_SH3_3	132	138	PF00018	0.508
LIG_SH3_3	36	42	PF00018	0.666
LIG_SUMO_SIM_par_1	14	20	PF11976	0.279
LIG_SUMO_SIM_par_1	66	73	PF11976	0.642
LIG_TRAF2_1	106	109	PF00917	0.466
LIG_WRC_WIRS_1	168	173	PF05994	0.472
MOD_CK1_1	58	64	PF00069	0.655
MOD_CK2_1	103	109	PF00069	0.466
MOD_CK2_1	82	88	PF00069	0.551
MOD_GlcNHglycan	130	133	PF01048	0.345
MOD_GlcNHglycan	25	28	PF01048	0.208
MOD_GlcNHglycan	5	9	PF01048	0.734
MOD_GlcNHglycan	58	61	PF01048	0.493
MOD_GSK3_1	51	58	PF00069	0.790
MOD_GSK3_1	59	66	PF00069	0.701
MOD_N-GLC_1	128	133	PF02516	0.308
MOD_NEK2_1	128	133	PF00069	0.466
MOD_NEK2_1	167	172	PF00069	0.472
MOD_NEK2_1	17	22	PF00069	0.315
MOD_PIKK_1	103	109	PF00454	0.508
MOD_PKA_2	103	109	PF00069	0.466
MOD_Plk_1	128	134	PF00069	0.481
MOD_Plk_1	51	57	PF00069	0.704
MOD_Plk_4	151	157	PF00069	0.466
MOD_Plk_4	82	88	PF00069	0.512
MOD_ProDKin_1	63	69	PF00069	0.734
TRG_DiLeu_BaLyEn_6	163	168	PF01217	0.454
TRG_DiLeu_BaLyEn_6	172	177	PF01217	0.422
TRG_Pf-PMV_PEXEL_1	175	179	PF00026	0.379

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1IM68	Leptomonas seymouri	49%	79%
A0A3S7WUN9	Leishmania donovani	86%	100%
A4H991	Leishmania braziliensis	60%	82%
A4HXL2	Leishmania infantum	87%	100%
E9ARA8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	83%	100%