Ubiquitin thioesterase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

otubain cysteine peptidase, Clan CA, family C65, putative

Species:

Leishmania major

UniProt:

Q4QE49_LEIMA

TriTrypDb:

LmjF.17.1400 , LMJLV39_170021600 , LMJSD75_170021300

Length:

270

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 3
Silverman et al.	no	yes: 0
Pissara et al.	yes	yes: 8

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	5
GO:0043226	organelle	2	5
GO:0043227	membrane-bounded organelle	3	5
GO:0043229	intracellular organelle	3	5
GO:0043231	intracellular membrane-bounded organelle	4	5
GO:0110165	cellular anatomical entity	1	5

Expansion

Sequence features

Q4QE49

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4QE49

Function

Biological processes
Term	Name	Level	Count
GO:0006508	proteolysis	4	11
GO:0006807	nitrogen compound metabolic process	2	11
GO:0008152	metabolic process	1	11
GO:0016579	protein deubiquitination	6	7
GO:0019538	protein metabolic process	3	11
GO:0036211	protein modification process	4	7
GO:0043170	macromolecule metabolic process	3	11
GO:0043412	macromolecule modification	4	7
GO:0044238	primary metabolic process	2	11
GO:0070646	protein modification by small protein removal	5	7
GO:0070647	protein modification by small protein conjugation or removal	5	7
GO:0071108	protein K48-linked deubiquitination	7	5
GO:0071704	organic substance metabolic process	2	11
GO:1901564	organonitrogen compound metabolic process	3	11

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	11
GO:0004843	cysteine-type deubiquitinase activity	5	11
GO:0005488	binding	1	5
GO:0005515	protein binding	2	5
GO:0008233	peptidase activity	3	11
GO:0008234	cysteine-type peptidase activity	4	11
GO:0016787	hydrolase activity	2	11
GO:0019783	ubiquitin-like protein peptidase activity	4	11
GO:0032182	ubiquitin-like protein binding	3	5
GO:0043130	ubiquitin binding	4	5
GO:0101005	deubiquitinase activity	5	11
GO:0140096	catalytic activity, acting on a protein	2	11

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	125	129	PF00656	0.488
CLV_C14_Caspase3-7	243	247	PF00656	0.481
CLV_C14_Caspase3-7	75	79	PF00656	0.488
CLV_NRD_NRD_1	18	20	PF00675	0.606
CLV_NRD_NRD_1	76	78	PF00675	0.343
CLV_PCSK_KEX2_1	18	20	PF00082	0.596
CLV_PCSK_KEX2_1	76	78	PF00082	0.343
CLV_PCSK_SKI1_1	140	144	PF00082	0.442
DEG_Nend_UBRbox_2	1	3	PF02207	0.728
DOC_MAPK_MEF2A_6	216	224	PF00069	0.362
DOC_PP2B_LxvP_1	256	259	PF13499	0.455
DOC_PP2B_LxvP_1	29	32	PF13499	0.459
DOC_USP7_MATH_1	181	185	PF00917	0.410
DOC_WW_Pin1_4	57	62	PF00397	0.444
LIG_14-3-3_CanoR_1	151	160	PF00244	0.440
LIG_BRCT_BRCA1_1	115	119	PF00533	0.488
LIG_Clathr_ClatBox_1	121	125	PF01394	0.410
LIG_DLG_GKlike_1	111	118	PF00625	0.488
LIG_FHA_1	125	131	PF00498	0.419
LIG_FHA_1	51	57	PF00498	0.440
LIG_LIR_Gen_1	103	108	PF02991	0.464
LIG_LIR_LC3C_4	249	253	PF02991	0.454
LIG_LIR_Nem_3	103	107	PF02991	0.421
LIG_LIR_Nem_3	116	122	PF02991	0.277
LIG_LIR_Nem_3	176	182	PF02991	0.488
LIG_LIR_Nem_3	68	72	PF02991	0.359
LIG_LYPXL_yS_3	24	27	PF13949	0.410
LIG_Pex14_2	122	126	PF04695	0.343
LIG_Pex14_2	214	218	PF04695	0.362
LIG_RPA_C_Fungi	106	118	PF08784	0.488
LIG_SH2_CRK	188	192	PF00017	0.455
LIG_SH2_CRK	262	266	PF00017	0.343
LIG_SH2_PTP2	104	107	PF00017	0.488
LIG_SH2_SRC	166	169	PF00017	0.461
LIG_SH2_STAT3	49	52	PF00017	0.455
LIG_SH2_STAT5	104	107	PF00017	0.416
LIG_SH2_STAT5	159	162	PF00017	0.351
LIG_SH2_STAT5	166	169	PF00017	0.332
LIG_SH2_STAT5	171	174	PF00017	0.325
LIG_SH2_STAT5	194	197	PF00017	0.492
LIG_SH2_STAT5	49	52	PF00017	0.455
LIG_SH3_3	92	98	PF00018	0.455
LIG_SUMO_SIM_anti_2	103	110	PF11976	0.488
LIG_SUMO_SIM_anti_2	198	203	PF11976	0.488
LIG_SUMO_SIM_par_1	25	30	PF11976	0.426
LIG_TRAF2_1	110	113	PF00917	0.410
LIG_TYR_ITIM	186	191	PF00017	0.362
MOD_CK1_1	60	66	PF00069	0.488
MOD_CK2_1	107	113	PF00069	0.474
MOD_GlcNHglycan	168	171	PF01048	0.444
MOD_GlcNHglycan	183	186	PF01048	0.397
MOD_GSK3_1	107	114	PF00069	0.416
MOD_GSK3_1	57	64	PF00069	0.442
MOD_GSK3_1	68	75	PF00069	0.190
MOD_N-GLC_1	233	238	PF02516	0.438
MOD_N-GLC_2	219	221	PF02516	0.455
MOD_NEK2_1	27	32	PF00069	0.383
MOD_NEK2_1	50	55	PF00069	0.484
MOD_PKA_2	72	78	PF00069	0.434
MOD_Plk_1	111	117	PF00069	0.488
MOD_Plk_1	138	144	PF00069	0.419
MOD_Plk_1	236	242	PF00069	0.450
MOD_Plk_4	34	40	PF00069	0.380
MOD_Plk_4	52	58	PF00069	0.288
MOD_ProDKin_1	57	63	PF00069	0.444
TRG_DiLeu_BaEn_1	103	108	PF01217	0.488
TRG_DiLeu_BaEn_3	177	183	PF01217	0.410
TRG_DiLeu_BaLyEn_6	22	27	PF01217	0.444
TRG_ENDOCYTIC_2	104	107	PF00928	0.455
TRG_ENDOCYTIC_2	149	152	PF00928	0.455
TRG_ENDOCYTIC_2	179	182	PF00928	0.428
TRG_ENDOCYTIC_2	188	191	PF00928	0.321
TRG_ENDOCYTIC_2	24	27	PF00928	0.481
TRG_ENDOCYTIC_2	262	265	PF00928	0.356
TRG_ER_diArg_1	17	19	PF00400	0.534
TRG_NES_CRM1_1	113	125	PF08389	0.410
TRG_Pf-PMV_PEXEL_1	151	155	PF00026	0.492

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I1P6	Leptomonas seymouri	62%	99%
A0A0S4J6F9	Bodo saltans	30%	100%
A0A1X0NSN1	Trypanosomatidae	36%	100%
A0A3S7WUP0	Leishmania donovani	98%	100%
A0A422NBK6	Trypanosoma rangeli	40%	100%
A4H997	Leishmania braziliensis	84%	100%
A4HXL8	Leishmania infantum	98%	100%
A8XDJ2	Caenorhabditis briggsae	33%	94%
B2RYG6	Rattus norvegicus	33%	100%
C9ZPB3	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	34%	100%
E9ARB4	Leishmania mexicana (strain MHOM/GT/2001/U1103)	96%	100%
Q7TQI3	Mus musculus	33%	100%
Q8LG98	Arabidopsis thaliana	29%	88%
Q96DC9	Homo sapiens	26%	100%
Q96FW1	Homo sapiens	33%	100%
Q9CQX0	Mus musculus	28%	100%
Q9VL00	Drosophila melanogaster	28%	100%
Q9XVR6	Caenorhabditis elegans	29%	95%
V5BNY1	Trypanosoma cruzi	40%	100%