3Beta_HSD domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

3-beta hydroxysteroid dehydrogenase/isomerase family, putative

Species:

Leishmania major

UniProt:

Q4QE34_LEIMA

TriTrypDb:

LmjF.18.0080 , LMJLV39_180006000 , LMJSD75_180006000

Length:

479

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005739	mitochondrion	5	2
GO:0043226	organelle	2	2
GO:0043227	membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043231	intracellular membrane-bounded organelle	4	2
GO:0110165	cellular anatomical entity	1	2

Expansion

Sequence features

Q4QE34

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4QE34

Function

Biological processes
Term	Name	Level	Count
GO:0006629	lipid metabolic process	3	11
GO:0006694	steroid biosynthetic process	5	11
GO:0006743	ubiquinone metabolic process	5	2
GO:0006744	ubiquinone biosynthetic process	6	2
GO:0008152	metabolic process	1	11
GO:0008202	steroid metabolic process	4	11
GO:0008610	lipid biosynthetic process	4	11
GO:0009058	biosynthetic process	2	11
GO:0009987	cellular process	1	2
GO:0042180	cellular ketone metabolic process	3	2
GO:0042181	ketone biosynthetic process	4	2
GO:0044237	cellular metabolic process	2	2
GO:0044238	primary metabolic process	2	11
GO:0044249	cellular biosynthetic process	3	2
GO:0044281	small molecule metabolic process	2	2
GO:0044283	small molecule biosynthetic process	3	2
GO:0071704	organic substance metabolic process	2	11
GO:1901004	ubiquinone-6 metabolic process	6	2
GO:1901006	ubiquinone-6 biosynthetic process	7	2
GO:1901360	organic cyclic compound metabolic process	3	11
GO:1901362	organic cyclic compound biosynthetic process	4	11
GO:1901576	organic substance biosynthetic process	3	11
GO:1901661	quinone metabolic process	4	2
GO:1901663	quinone biosynthetic process	5	2

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	11
GO:0003854	3-beta-hydroxy-delta5-steroid dehydrogenase activity	5	11
GO:0005488	binding	1	2
GO:0016229	steroid dehydrogenase activity	3	11
GO:0016491	oxidoreductase activity	2	11
GO:0016614	oxidoreductase activity, acting on CH-OH group of donors	3	11
GO:0016616	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor	4	11
GO:0033764	steroid dehydrogenase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor	4	11
GO:0044877	protein-containing complex binding	2	2
GO:0016853	isomerase activity	2	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	130	134	PF00656	0.348
CLV_NRD_NRD_1	35	37	PF00675	0.545
CLV_NRD_NRD_1	361	363	PF00675	0.430
CLV_NRD_NRD_1	392	394	PF00675	0.539
CLV_NRD_NRD_1	80	82	PF00675	0.534
CLV_PCSK_KEX2_1	319	321	PF00082	0.506
CLV_PCSK_KEX2_1	361	363	PF00082	0.430
CLV_PCSK_KEX2_1	79	81	PF00082	0.542
CLV_PCSK_PC1ET2_1	319	321	PF00082	0.450
CLV_PCSK_PC7_1	315	321	PF00082	0.496
CLV_PCSK_SKI1_1	203	207	PF00082	0.311
CLV_PCSK_SKI1_1	335	339	PF00082	0.502
CLV_PCSK_SKI1_1	413	417	PF00082	0.447
CLV_PCSK_SKI1_1	85	89	PF00082	0.396
CLV_Separin_Metazoa	102	106	PF03568	0.279
DEG_APCC_DBOX_1	35	43	PF00400	0.519
DEG_Nend_UBRbox_3	1	3	PF02207	0.676
DOC_MAPK_gen_1	197	206	PF00069	0.279
DOC_PP1_RVXF_1	201	207	PF00149	0.279
DOC_PP2B_LxvP_1	321	324	PF13499	0.435
DOC_PP4_FxxP_1	21	24	PF00568	0.541
DOC_SPAK_OSR1_1	3	7	PF12202	0.572
DOC_USP7_MATH_1	127	131	PF00917	0.259
DOC_USP7_MATH_1	160	164	PF00917	0.418
DOC_USP7_MATH_1	177	181	PF00917	0.279
DOC_USP7_MATH_1	215	219	PF00917	0.362
DOC_USP7_UBL2_3	138	142	PF12436	0.228
DOC_WW_Pin1_4	375	380	PF00397	0.484
LIG_14-3-3_CanoR_1	117	125	PF00244	0.311
LIG_14-3-3_CanoR_1	284	294	PF00244	0.350
LIG_14-3-3_CanoR_1	3	7	PF00244	0.684
LIG_14-3-3_CanoR_1	361	366	PF00244	0.457
LIG_14-3-3_CanoR_1	368	373	PF00244	0.368
LIG_AP2alpha_1	21	25	PF02296	0.615
LIG_AP2alpha_2	23	25	PF02296	0.606
LIG_BIR_III_4	229	233	PF00653	0.384
LIG_BRCT_BRCA1_1	362	366	PF00533	0.473
LIG_eIF4E_1	62	68	PF01652	0.515
LIG_FHA_1	149	155	PF00498	0.311
LIG_FHA_1	271	277	PF00498	0.308
LIG_FHA_1	336	342	PF00498	0.500
LIG_FHA_1	351	357	PF00498	0.333
LIG_FHA_1	438	444	PF00498	0.521
LIG_FHA_1	63	69	PF00498	0.637
LIG_FHA_2	18	24	PF00498	0.678
LIG_FHA_2	362	368	PF00498	0.468
LIG_FHA_2	379	385	PF00498	0.353
LIG_LIR_Apic_2	20	24	PF02991	0.621
LIG_LIR_Apic_2	338	342	PF02991	0.365
LIG_LIR_Gen_1	217	225	PF02991	0.342
LIG_LIR_Gen_1	363	370	PF02991	0.439
LIG_LIR_Gen_1	371	379	PF02991	0.359
LIG_LIR_LC3C_4	318	323	PF02991	0.442
LIG_LIR_Nem_3	119	125	PF02991	0.279
LIG_LIR_Nem_3	20	25	PF02991	0.656
LIG_LIR_Nem_3	217	223	PF02991	0.367
LIG_LIR_Nem_3	308	313	PF02991	0.362
LIG_LIR_Nem_3	363	369	PF02991	0.453
LIG_LIR_Nem_3	371	375	PF02991	0.354
LIG_LIR_Nem_3	402	408	PF02991	0.380
LIG_LYPXL_yS_3	122	125	PF13949	0.279
LIG_MYND_1	123	127	PF01753	0.258
LIG_NRBOX	131	137	PF00104	0.279
LIG_Pex14_2	21	25	PF04695	0.615
LIG_Pex14_2	329	333	PF04695	0.429
LIG_PTB_Apo_2	340	347	PF02174	0.375
LIG_REV1ctd_RIR_1	330	339	PF16727	0.406
LIG_SH2_CRK	253	257	PF00017	0.384
LIG_SH2_GRB2like	176	179	PF00017	0.279
LIG_SH2_PTP2	259	262	PF00017	0.395
LIG_SH2_SRC	259	262	PF00017	0.370
LIG_SH2_STAT3	43	46	PF00017	0.591
LIG_SH2_STAT3	451	454	PF00017	0.491
LIG_SH2_STAT5	220	223	PF00017	0.355
LIG_SH2_STAT5	259	262	PF00017	0.382
LIG_SH2_STAT5	350	353	PF00017	0.453
LIG_SH2_STAT5	377	380	PF00017	0.477
LIG_SH2_STAT5	98	101	PF00017	0.279
LIG_SH3_3	338	344	PF00018	0.417
LIG_SH3_3	467	473	PF00018	0.529
LIG_SH3_3	70	76	PF00018	0.577
LIG_TRAF2_1	397	400	PF00917	0.531
LIG_TRAF2_2	397	402	PF00917	0.314
LIG_WRC_WIRS_1	351	356	PF05994	0.468
MOD_CK1_1	468	474	PF00069	0.605
MOD_CK1_1	5	11	PF00069	0.685
MOD_CK2_1	302	308	PF00069	0.360
MOD_CK2_1	378	384	PF00069	0.365
MOD_GlcNHglycan	7	10	PF01048	0.607
MOD_GlcNHglycan	95	98	PF01048	0.279
MOD_GSK3_1	117	124	PF00069	0.297
MOD_GSK3_1	7	14	PF00069	0.659
MOD_N-GLC_1	160	165	PF02516	0.279
MOD_N-GLC_1	177	182	PF02516	0.279
MOD_N-GLC_1	190	195	PF02516	0.279
MOD_N-GLC_1	468	473	PF02516	0.560
MOD_NEK2_1	210	215	PF00069	0.286
MOD_NEK2_1	93	98	PF00069	0.279
MOD_PIKK_1	265	271	PF00454	0.453
MOD_PIKK_1	42	48	PF00454	0.587
MOD_PIKK_1	468	474	PF00454	0.556
MOD_PKA_1	361	367	PF00069	0.463
MOD_PKA_2	116	122	PF00069	0.311
MOD_PKA_2	2	8	PF00069	0.638
MOD_PKA_2	283	289	PF00069	0.330
MOD_PKA_2	360	366	PF00069	0.442
MOD_Plk_1	190	196	PF00069	0.316
MOD_Plk_1	210	216	PF00069	0.150
MOD_Plk_1	236	242	PF00069	0.339
MOD_Plk_4	121	127	PF00069	0.283
MOD_Plk_4	162	168	PF00069	0.283
MOD_Plk_4	236	242	PF00069	0.339
MOD_Plk_4	270	276	PF00069	0.318
MOD_Plk_4	28	34	PF00069	0.584
MOD_Plk_4	361	367	PF00069	0.463
MOD_Plk_4	62	68	PF00069	0.632
MOD_Plk_4	88	94	PF00069	0.336
MOD_ProDKin_1	375	381	PF00069	0.485
MOD_SUMO_for_1	431	434	PF00179	0.474
MOD_SUMO_rev_2	30	39	PF00179	0.537
TRG_ENDOCYTIC_2	122	125	PF00928	0.279
TRG_ENDOCYTIC_2	220	223	PF00928	0.333
TRG_ENDOCYTIC_2	253	256	PF00928	0.416
TRG_ENDOCYTIC_2	326	329	PF00928	0.393
TRG_ER_diArg_1	223	226	PF00400	0.342
TRG_ER_diArg_1	79	81	PF00400	0.542
TRG_Pf-PMV_PEXEL_1	105	109	PF00026	0.357

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PDT9	Leptomonas seymouri	90%	100%
A0A0S4JJB1	Bodo saltans	76%	100%
A0A1X0P6V3	Trypanosomatidae	84%	100%
A0A3Q8ICY3	Leishmania donovani	98%	100%
A4H9B3	Leishmania braziliensis	94%	100%
A4HXN4	Leishmania infantum	98%	100%
D0A011	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	86%	99%
E9ARE5	Leishmania mexicana (strain MHOM/GT/2001/U1103)	97%	100%
V5B9X0	Trypanosoma cruzi	84%	100%