Putative cysteine peptidase, Clan CD,family C13

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

GPI-anchor transamidase subunit 8 (GPI8), putative

Species:

Leishmania major

UniProt:

Q4QE06_LEIMA

TriTrypDb:

LmjF.18.0360 , LMJLV39_180009000 , LMJSD75_180008900

Length:

357

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 6, no: 4
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005635	nuclear envelope	4	2
GO:0005783	endoplasmic reticulum	5	2
GO:0008303	caspase complex	5	11
GO:0031967	organelle envelope	3	2
GO:0031975	envelope	2	2
GO:0032991	protein-containing complex	1	11
GO:0042765	GPI-anchor transamidase complex	3	11
GO:0043226	organelle	2	2
GO:0043227	membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043231	intracellular membrane-bounded organelle	4	2
GO:0098796	membrane protein complex	2	11
GO:0110165	cellular anatomical entity	1	2
GO:0140534	endoplasmic reticulum protein-containing complex	2	11
GO:1902494	catalytic complex	2	11
GO:1905368	peptidase complex	3	11
GO:1905369	endopeptidase complex	4	11

Expansion

Sequence features

Q4QE06

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4QE06

Function

Biological processes
Term	Name	Level	Count
GO:0006508	proteolysis	4	11
GO:0006807	nitrogen compound metabolic process	2	11
GO:0008104	protein localization	4	2
GO:0008152	metabolic process	1	11
GO:0009987	cellular process	1	2
GO:0016255	attachment of GPI anchor to protein	5	11
GO:0019538	protein metabolic process	3	11
GO:0033036	macromolecule localization	2	2
GO:0034394	protein localization to cell surface	5	2
GO:0036211	protein modification process	4	11
GO:0043170	macromolecule metabolic process	3	11
GO:0043412	macromolecule modification	4	11
GO:0044238	primary metabolic process	2	11
GO:0051179	localization	1	2
GO:0051641	cellular localization	2	2
GO:0070727	cellular macromolecule localization	3	2
GO:0071704	organic substance metabolic process	2	11
GO:1901564	organonitrogen compound metabolic process	3	11

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	11
GO:0003923	GPI-anchor transamidase activity	6	11
GO:0004175	endopeptidase activity	4	11
GO:0004197	cysteine-type endopeptidase activity	5	11
GO:0008233	peptidase activity	3	11
GO:0008234	cysteine-type peptidase activity	4	11
GO:0016787	hydrolase activity	2	11
GO:0140096	catalytic activity, acting on a protein	2	11

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	147	149	PF00675	0.425
CLV_NRD_NRD_1	165	167	PF00675	0.412
CLV_NRD_NRD_1	212	214	PF00675	0.413
CLV_NRD_NRD_1	286	288	PF00675	0.627
CLV_PCSK_KEX2_1	147	149	PF00082	0.425
CLV_PCSK_KEX2_1	165	167	PF00082	0.412
CLV_PCSK_KEX2_1	212	214	PF00082	0.425
CLV_PCSK_KEX2_1	286	288	PF00082	0.624
CLV_PCSK_SKI1_1	147	151	PF00082	0.425
CLV_PCSK_SKI1_1	165	169	PF00082	0.523
CLV_PCSK_SKI1_1	216	220	PF00082	0.425
CLV_PCSK_SKI1_1	64	68	PF00082	0.425
DEG_APCC_DBOX_1	146	154	PF00400	0.225
DOC_CYCLIN_RxL_1	162	171	PF00134	0.307
DOC_MAPK_gen_1	212	219	PF00069	0.212
DOC_MAPK_MEF2A_6	172	179	PF00069	0.211
DOC_PP1_RVXF_1	214	220	PF00149	0.227
DOC_PP4_FxxP_1	113	116	PF00568	0.293
DOC_USP7_MATH_1	39	43	PF00917	0.443
DOC_USP7_MATH_2	109	115	PF00917	0.227
DOC_USP7_UBL2_3	185	189	PF12436	0.307
DOC_WW_Pin1_4	158	163	PF00397	0.225
LIG_14-3-3_CanoR_1	147	153	PF00244	0.307
LIG_14-3-3_CanoR_1	165	175	PF00244	0.243
LIG_14-3-3_CanoR_1	6	15	PF00244	0.427
LIG_14-3-3_CanoR_1	70	78	PF00244	0.212
LIG_BRCT_BRCA1_1	22	26	PF00533	0.391
LIG_BRCT_BRCA1_1	96	100	PF00533	0.307
LIG_deltaCOP1_diTrp_1	333	339	PF00928	0.308
LIG_FHA_1	162	168	PF00498	0.307
LIG_FHA_1	191	197	PF00498	0.305
LIG_FHA_1	259	265	PF00498	0.227
LIG_FHA_1	283	289	PF00498	0.418
LIG_FHA_1	8	14	PF00498	0.319
LIG_LIR_Apic_2	111	116	PF02991	0.251
LIG_LIR_Gen_1	28	38	PF02991	0.386
LIG_LIR_Gen_1	53	60	PF02991	0.211
LIG_LIR_Gen_1	77	87	PF02991	0.307
LIG_LIR_LC3C_4	10	15	PF02991	0.246
LIG_LIR_Nem_3	186	190	PF02991	0.224
LIG_LIR_Nem_3	270	275	PF02991	0.286
LIG_LIR_Nem_3	28	33	PF02991	0.358
LIG_LIR_Nem_3	299	303	PF02991	0.239
LIG_LIR_Nem_3	53	59	PF02991	0.233
LIG_LIR_Nem_3	77	82	PF02991	0.277
LIG_SH2_CRK	214	218	PF00017	0.225
LIG_SH2_CRK	272	276	PF00017	0.272
LIG_SH2_CRK	30	34	PF00017	0.440
LIG_SH2_CRK	79	83	PF00017	0.307
LIG_SH2_STAP1	251	255	PF00017	0.243
LIG_SH2_STAT3	251	254	PF00017	0.293
LIG_SH2_STAT3	255	258	PF00017	0.293
LIG_SH2_STAT5	178	181	PF00017	0.250
LIG_SH2_STAT5	187	190	PF00017	0.252
LIG_SH2_STAT5	65	68	PF00017	0.225
LIG_SH3_3	230	236	PF00018	0.258
LIG_SH3_3	322	328	PF00018	0.290
LIG_Sin3_3	9	16	PF02671	0.233
LIG_SUMO_SIM_anti_2	10	16	PF11976	0.374
LIG_SUMO_SIM_anti_2	291	297	PF11976	0.356
LIG_SUMO_SIM_par_1	16	21	PF11976	0.228
LIG_WRC_WIRS_1	263	268	PF05994	0.246
MOD_CDC14_SPxK_1	3	6	PF00782	0.542
MOD_CDK_SPxxK_3	158	165	PF00069	0.225
MOD_CK1_1	199	205	PF00069	0.258
MOD_CK2_1	241	247	PF00069	0.270
MOD_GlcNHglycan	133	136	PF01048	0.490
MOD_GlcNHglycan	41	44	PF01048	0.673
MOD_GSK3_1	166	173	PF00069	0.218
MOD_GSK3_1	190	197	PF00069	0.226
MOD_GSK3_1	241	248	PF00069	0.292
MOD_GSK3_1	258	265	PF00069	0.292
MOD_GSK3_1	278	285	PF00069	0.227
MOD_GSK3_1	310	317	PF00069	0.427
MOD_N-GLC_1	275	280	PF02516	0.535
MOD_N-GLC_1	314	319	PF02516	0.604
MOD_NEK2_1	18	23	PF00069	0.358
MOD_NEK2_1	220	225	PF00069	0.242
MOD_NEK2_1	275	280	PF00069	0.310
MOD_NEK2_1	296	301	PF00069	0.250
MOD_NEK2_1	7	12	PF00069	0.337
MOD_NEK2_1	96	101	PF00069	0.272
MOD_PIKK_1	111	117	PF00454	0.247
MOD_PK_1	148	154	PF00069	0.307
MOD_PKA_2	5	11	PF00069	0.432
MOD_PKA_2	69	75	PF00069	0.212
MOD_Plk_4	148	154	PF00069	0.295
MOD_Plk_4	183	189	PF00069	0.244
MOD_Plk_4	201	207	PF00069	0.138
MOD_Plk_4	262	268	PF00069	0.259
MOD_Plk_4	270	276	PF00069	0.263
MOD_Plk_4	291	297	PF00069	0.323
MOD_Plk_4	74	80	PF00069	0.258
MOD_ProDKin_1	158	164	PF00069	0.225
MOD_SUMO_rev_2	169	173	PF00179	0.211
TRG_DiLeu_BaEn_1	270	275	PF01217	0.249
TRG_DiLeu_BaLyEn_6	163	168	PF01217	0.307
TRG_ENDOCYTIC_2	187	190	PF00928	0.338
TRG_ENDOCYTIC_2	214	217	PF00928	0.258
TRG_ENDOCYTIC_2	272	275	PF00928	0.299
TRG_ENDOCYTIC_2	30	33	PF00928	0.436
TRG_ENDOCYTIC_2	79	82	PF00928	0.312
TRG_ER_diArg_1	146	148	PF00400	0.225
TRG_ER_diArg_1	165	167	PF00400	0.212
TRG_ER_diArg_1	212	214	PF00400	0.225
TRG_ER_diArg_1	286	288	PF00400	0.356
TRG_NES_CRM1_1	258	270	PF08389	0.224
TRG_Pf-PMV_PEXEL_1	165	170	PF00026	0.412

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1IJ31	Leptomonas seymouri	80%	98%
A0A1X0P6S3	Trypanosomatidae	54%	100%
A0A3S5H727	Leishmania donovani	91%	100%
A4H9E0	Leishmania braziliensis	80%	100%
A4HXR1	Leishmania infantum	91%	100%
B6DTF5	Bodo saltans	39%	100%
D0A043	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	54%	100%
E9ARH3	Leishmania mexicana (strain MHOM/GT/2001/U1103)	88%	100%
O89017	Mus musculus	27%	82%
P49044	Vicia sativa	24%	72%
Q8T4E1	Drosophila melanogaster	33%	100%
Q95M12	Bos taurus	27%	82%
Q99538	Homo sapiens	27%	82%
Q9LJX8	Arabidopsis thaliana	26%	77%
Q9USP5	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	36%	94%
V5BWA3	Trypanosoma cruzi	55%	100%