Putative DNA-directed RNA polymerases II

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

DNA-directed RNA polymerase II/III subunit, putative

Species:

Leishmania major

UniProt:

Q4QDW4_LEIMA

TriTrypDb:

LmjF.18.0780 , LMJLV39_180013300 , LMJSD75_180013200

Length:

239

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0000428	DNA-directed RNA polymerase complex	4	12
GO:0005634	nucleus	5	2
GO:0005665	RNA polymerase II, core complex	4	2
GO:0005666	RNA polymerase III complex	4	2
GO:0005736	RNA polymerase I complex	4	2
GO:0030880	RNA polymerase complex	3	12
GO:0032991	protein-containing complex	1	12
GO:0043226	organelle	2	2
GO:0043227	membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043231	intracellular membrane-bounded organelle	4	2
GO:0055029	nuclear DNA-directed RNA polymerase complex	3	12
GO:0061695	transferase complex, transferring phosphorus-containing groups	4	12
GO:0110165	cellular anatomical entity	1	2
GO:0140513	nuclear protein-containing complex	2	12
GO:0140535	intracellular protein-containing complex	2	12
GO:1902494	catalytic complex	2	12
GO:1990234	transferase complex	3	12

Expansion

Sequence features

Q4QDW4

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4QDW4

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	12
GO:0006351	DNA-templated transcription	7	12
GO:0006725	cellular aromatic compound metabolic process	3	12
GO:0006807	nitrogen compound metabolic process	2	12
GO:0008152	metabolic process	1	12
GO:0009058	biosynthetic process	2	12
GO:0009059	macromolecule biosynthetic process	4	12
GO:0009987	cellular process	1	12
GO:0016070	RNA metabolic process	5	12
GO:0018130	heterocycle biosynthetic process	4	12
GO:0019438	aromatic compound biosynthetic process	4	12
GO:0032774	RNA biosynthetic process	5	12
GO:0034641	cellular nitrogen compound metabolic process	3	12
GO:0034654	nucleobase-containing compound biosynthetic process	4	12
GO:0043170	macromolecule metabolic process	3	12
GO:0044237	cellular metabolic process	2	12
GO:0044238	primary metabolic process	2	12
GO:0044249	cellular biosynthetic process	3	12
GO:0044271	cellular nitrogen compound biosynthetic process	4	12
GO:0046483	heterocycle metabolic process	3	12
GO:0071704	organic substance metabolic process	2	12
GO:0090304	nucleic acid metabolic process	4	12
GO:0097659	nucleic acid-templated transcription	6	12
GO:1901360	organic cyclic compound metabolic process	3	12
GO:1901362	organic cyclic compound biosynthetic process	4	12
GO:1901576	organic substance biosynthetic process	3	12

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	12
GO:0003677	DNA binding	4	12
GO:0003824	catalytic activity	1	12
GO:0003899	DNA-directed 5'-3' RNA polymerase activity	6	12
GO:0005488	binding	1	12
GO:0016740	transferase activity	2	12
GO:0016772	transferase activity, transferring phosphorus-containing groups	3	12
GO:0016779	nucleotidyltransferase activity	4	12
GO:0034062	5'-3' RNA polymerase activity	5	12
GO:0097159	organic cyclic compound binding	2	12
GO:0097747	RNA polymerase activity	4	12
GO:0140098	catalytic activity, acting on RNA	3	12
GO:0140640	catalytic activity, acting on a nucleic acid	2	12
GO:1901363	heterocyclic compound binding	2	12
GO:0003887	DNA-directed DNA polymerase activity	5	1
GO:0034061	DNA polymerase activity	4	1
GO:0140097	catalytic activity, acting on DNA	3	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	140	142	PF00675	0.479
CLV_NRD_NRD_1	215	217	PF00675	0.224
CLV_NRD_NRD_1	223	225	PF00675	0.206
CLV_NRD_NRD_1	27	29	PF00675	0.285
CLV_NRD_NRD_1	72	74	PF00675	0.414
CLV_NRD_NRD_1	78	80	PF00675	0.443
CLV_PCSK_KEX2_1	215	217	PF00082	0.267
CLV_PCSK_KEX2_1	72	74	PF00082	0.381
CLV_PCSK_KEX2_1	78	80	PF00082	0.292
CLV_PCSK_SKI1_1	127	131	PF00082	0.467
CLV_PCSK_SKI1_1	186	190	PF00082	0.194
DEG_SCF_FBW7_2	175	182	PF00400	0.454
DOC_ANK_TNKS_1	87	94	PF00023	0.393
DOC_CKS1_1	130	135	PF01111	0.411
DOC_MAPK_gen_1	215	222	PF00069	0.467
DOC_MAPK_gen_1	78	84	PF00069	0.332
DOC_PP1_RVXF_1	125	131	PF00149	0.454
DOC_PP1_RVXF_1	6	12	PF00149	0.302
DOC_PP4_FxxP_1	102	105	PF00568	0.393
DOC_PP4_FxxP_1	130	133	PF00568	0.455
DOC_WW_Pin1_4	129	134	PF00397	0.463
DOC_WW_Pin1_4	175	180	PF00397	0.502
LIG_14-3-3_CanoR_1	141	145	PF00244	0.464
LIG_14-3-3_CanoR_1	16	25	PF00244	0.287
LIG_14-3-3_CanoR_1	201	207	PF00244	0.413
LIG_14-3-3_CanoR_1	224	233	PF00244	0.413
LIG_APCC_ABBA_1	156	161	PF00400	0.362
LIG_APCC_ABBAyCdc20_2	155	161	PF00400	0.315
LIG_BIR_III_2	68	72	PF00653	0.326
LIG_FHA_1	203	209	PF00498	0.391
LIG_FHA_1	65	71	PF00498	0.181
LIG_FHA_2	167	173	PF00498	0.413
LIG_FHA_2	18	24	PF00498	0.250
LIG_LIR_Gen_1	123	133	PF02991	0.464
LIG_LIR_Gen_1	153	164	PF02991	0.469
LIG_LIR_Nem_3	123	128	PF02991	0.462
LIG_LIR_Nem_3	153	159	PF02991	0.472
LIG_LIR_Nem_3	234	238	PF02991	0.435
LIG_Pex14_2	11	15	PF04695	0.302
LIG_SH2_CRK	125	129	PF00017	0.458
LIG_SH2_CRK	235	239	PF00017	0.413
LIG_SH2_STAT5	101	104	PF00017	0.379
LIG_SH2_STAT5	232	235	PF00017	0.424
LIG_SH2_STAT5	48	51	PF00017	0.284
LIG_SH3_3	130	136	PF00018	0.479
LIG_TRAF2_1	181	184	PF00917	0.515
LIG_TYR_ITIM	233	238	PF00017	0.424
MOD_CDK_SPxK_1	129	135	PF00069	0.473
MOD_CK2_1	166	172	PF00069	0.413
MOD_CK2_1	17	23	PF00069	0.250
MOD_CK2_1	178	184	PF00069	0.468
MOD_CK2_1	37	43	PF00069	0.121
MOD_GlcNHglycan	20	23	PF01048	0.356
MOD_GlcNHglycan	227	230	PF01048	0.240
MOD_GSK3_1	143	150	PF00069	0.509
MOD_GSK3_1	162	169	PF00069	0.479
MOD_N-GLC_1	147	152	PF02516	0.429
MOD_N-GLC_1	166	171	PF02516	0.213
MOD_NEK2_1	231	236	PF00069	0.437
MOD_PKA_2	140	146	PF00069	0.441
MOD_PKA_2	223	229	PF00069	0.433
MOD_Plk_1	162	168	PF00069	0.479
MOD_ProDKin_1	129	135	PF00069	0.473
MOD_ProDKin_1	175	181	PF00069	0.502
TRG_ENDOCYTIC_2	125	128	PF00928	0.440
TRG_ENDOCYTIC_2	211	214	PF00928	0.441
TRG_ENDOCYTIC_2	235	238	PF00928	0.441
TRG_ER_diArg_1	214	216	PF00400	0.376
TRG_ER_diArg_1	71	73	PF00400	0.414
TRG_ER_diArg_1	77	79	PF00400	0.443
TRG_NES_CRM1_1	43	57	PF08389	0.375
TRG_Pf-PMV_PEXEL_1	201	206	PF00026	0.213

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PE97	Leptomonas seymouri	85%	100%
A0A0S4J1J5	Bodo saltans	27%	86%
A0A0S4JAI3	Bodo saltans	42%	100%
A0A1X0P8B1	Trypanosomatidae	49%	100%
A0A3Q8ID21	Leishmania donovani	97%	100%
A0A3R7KXJ2	Trypanosoma rangeli	30%	84%
A0A3R7M9Z6	Trypanosoma rangeli	50%	91%
A4H9I9	Leishmania braziliensis	95%	100%
A4H9J0	Leishmania braziliensis	35%	74%
A4HXV2	Leishmania infantum	97%	100%
A8XGH1	Caenorhabditis briggsae	32%	100%
B0BNE2	Rattus norvegicus	31%	100%
C9ZZV1	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	48%	100%
E9ARL5	Leishmania mexicana (strain MHOM/GT/2001/U1103)	97%	100%
E9ARL6	Leishmania mexicana (strain MHOM/GT/2001/U1103)	33%	70%
O81098	Arabidopsis thaliana	31%	100%
P19388	Homo sapiens	31%	100%
P20434	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	31%	100%
Q09191	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	30%	100%
Q2T9T3	Bos taurus	32%	100%
Q54EH2	Dictyostelium discoideum	36%	100%
Q5R587	Pongo abelii	31%	100%
Q6BIJ0	Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / BCRC 21394 / JCM 1990 / NBRC 0083 / IGC 2968)	32%	100%
Q6CA26	Yarrowia lipolytica (strain CLIB 122 / E 150)	28%	100%
Q6CJ62	Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37)	33%	100%
Q6FQA6	Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65)	32%	100%
Q757H7	Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056)	32%	100%
Q80UW8	Mus musculus	31%	100%
Q9FJL8	Arabidopsis thaliana	31%	100%
Q9M1J2	Arabidopsis thaliana	24%	100%
Q9N5K2	Caenorhabditis elegans	32%	100%
Q9P4B9	Kluyveromyces marxianus	32%	100%
Q9ZVB9	Arabidopsis thaliana	30%	100%
V5B6J4	Trypanosoma cruzi	29%	85%
V5BRU4	Trypanosoma cruzi	49%	100%