Putative DNA-directed RNA polymerase II

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

DNA-directed RNA polymerase I subunit RPB5z, putative

Species:

Leishmania major

UniProt:

Q4QDW3_LEIMA

TriTrypDb:

LmjF.18.0790 , LMJLV39_180013400 , LMJSD75_180013300

Length:

342

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0000428	DNA-directed RNA polymerase complex	4	11
GO:0005634	nucleus	5	2
GO:0005665	RNA polymerase II, core complex	4	2
GO:0005666	RNA polymerase III complex	4	2
GO:0005736	RNA polymerase I complex	4	2
GO:0005737	cytoplasm	2	2
GO:0030880	RNA polymerase complex	3	11
GO:0031974	membrane-enclosed lumen	2	2
GO:0031981	nuclear lumen	5	2
GO:0032838	plasma membrane bounded cell projection cytoplasm	4	2
GO:0032991	protein-containing complex	1	11
GO:0043226	organelle	2	2
GO:0043227	membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043231	intracellular membrane-bounded organelle	4	2
GO:0043233	organelle lumen	3	2
GO:0055029	nuclear DNA-directed RNA polymerase complex	3	9
GO:0061695	transferase complex, transferring phosphorus-containing groups	4	11
GO:0070013	intracellular organelle lumen	4	2
GO:0097014	ciliary plasm	5	2
GO:0099568	cytoplasmic region	3	2
GO:0110165	cellular anatomical entity	1	2
GO:0140513	nuclear protein-containing complex	2	9
GO:0140535	intracellular protein-containing complex	2	11
GO:1902494	catalytic complex	2	11
GO:1990234	transferase complex	3	11

Expansion

Sequence features

Q4QDW3

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4QDW3

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	11
GO:0006351	DNA-templated transcription	7	11
GO:0006725	cellular aromatic compound metabolic process	3	11
GO:0006807	nitrogen compound metabolic process	2	11
GO:0008152	metabolic process	1	11
GO:0009058	biosynthetic process	2	11
GO:0009059	macromolecule biosynthetic process	4	11
GO:0009987	cellular process	1	11
GO:0016070	RNA metabolic process	5	11
GO:0018130	heterocycle biosynthetic process	4	11
GO:0019438	aromatic compound biosynthetic process	4	11
GO:0032774	RNA biosynthetic process	5	11
GO:0034641	cellular nitrogen compound metabolic process	3	11
GO:0034654	nucleobase-containing compound biosynthetic process	4	11
GO:0043170	macromolecule metabolic process	3	11
GO:0044237	cellular metabolic process	2	11
GO:0044238	primary metabolic process	2	11
GO:0044249	cellular biosynthetic process	3	11
GO:0044271	cellular nitrogen compound biosynthetic process	4	11
GO:0046483	heterocycle metabolic process	3	11
GO:0071704	organic substance metabolic process	2	11
GO:0090304	nucleic acid metabolic process	4	11
GO:0097659	nucleic acid-templated transcription	6	11
GO:1901360	organic cyclic compound metabolic process	3	11
GO:1901362	organic cyclic compound biosynthetic process	4	11
GO:1901576	organic substance biosynthetic process	3	11

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	11
GO:0003677	DNA binding	4	11
GO:0003824	catalytic activity	1	11
GO:0003899	DNA-directed 5'-3' RNA polymerase activity	6	11
GO:0005488	binding	1	11
GO:0016740	transferase activity	2	11
GO:0016772	transferase activity, transferring phosphorus-containing groups	3	11
GO:0016779	nucleotidyltransferase activity	4	11
GO:0034062	5'-3' RNA polymerase activity	5	11
GO:0097159	organic cyclic compound binding	2	11
GO:0097747	RNA polymerase activity	4	11
GO:0140098	catalytic activity, acting on RNA	3	11
GO:0140640	catalytic activity, acting on a nucleic acid	2	11
GO:1901363	heterocyclic compound binding	2	11

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	95	99	PF00656	0.561
CLV_NRD_NRD_1	116	118	PF00675	0.538
CLV_NRD_NRD_1	139	141	PF00675	0.641
CLV_NRD_NRD_1	294	296	PF00675	0.344
CLV_NRD_NRD_1	87	89	PF00675	0.624
CLV_PCSK_KEX2_1	116	118	PF00082	0.496
CLV_PCSK_KEX2_1	139	141	PF00082	0.612
CLV_PCSK_KEX2_1	87	89	PF00082	0.624
CLV_PCSK_PC7_1	83	89	PF00082	0.544
CLV_PCSK_SKI1_1	139	143	PF00082	0.537
CLV_PCSK_SKI1_1	190	194	PF00082	0.563
CLV_PCSK_SKI1_1	217	221	PF00082	0.465
CLV_PCSK_SKI1_1	88	92	PF00082	0.692
DOC_MAPK_gen_1	190	200	PF00069	0.424
DOC_MAPK_gen_1	220	229	PF00069	0.483
DOC_MAPK_MEF2A_6	193	200	PF00069	0.384
DOC_MAPK_MEF2A_6	232	239	PF00069	0.574
DOC_MAPK_RevD_3	281	296	PF00069	0.491
DOC_USP7_MATH_1	68	72	PF00917	0.577
DOC_USP7_UBL2_3	186	190	PF12436	0.598
DOC_USP7_UBL2_3	205	209	PF12436	0.528
DOC_USP7_UBL2_3	217	221	PF12436	0.405
DOC_WW_Pin1_4	200	205	PF00397	0.448
DOC_WW_Pin1_4	281	286	PF00397	0.475
DOC_WW_Pin1_4	61	66	PF00397	0.443
LIG_14-3-3_CanoR_1	131	137	PF00244	0.479
LIG_14-3-3_CanoR_1	139	144	PF00244	0.645
LIG_14-3-3_CanoR_1	14	20	PF00244	0.294
LIG_APCC_ABBA_1	259	264	PF00400	0.343
LIG_BIR_II_1	1	5	PF00653	0.491
LIG_deltaCOP1_diTrp_1	53	61	PF00928	0.389
LIG_FHA_1	131	137	PF00498	0.446
LIG_FHA_1	228	234	PF00498	0.473
LIG_FHA_2	250	256	PF00498	0.496
LIG_FHA_2	282	288	PF00498	0.534
LIG_Integrin_RGD_1	77	79	PF01839	0.474
LIG_LIR_Gen_1	122	130	PF02991	0.458
LIG_LIR_Nem_3	332	338	PF02991	0.441
LIG_Pex14_2	57	61	PF04695	0.384
LIG_SH2_NCK_1	105	109	PF00017	0.435
LIG_SH2_NCK_1	176	180	PF00017	0.509
LIG_SH2_STAP1	105	109	PF00017	0.575
LIG_SH2_STAP1	176	180	PF00017	0.509
LIG_SH2_STAT5	28	31	PF00017	0.391
LIG_SH2_STAT5	314	317	PF00017	0.456
LIG_SH3_3	37	43	PF00018	0.453
LIG_SH3_3	59	65	PF00018	0.494
LIG_SUMO_SIM_par_1	224	230	PF11976	0.352
LIG_TRAF2_1	3	6	PF00917	0.436
LIG_TYR_ITIM	312	317	PF00017	0.440
LIG_WRC_WIRS_1	211	216	PF05994	0.405
MOD_CDC14_SPxK_1	64	67	PF00782	0.415
MOD_CDK_SPK_2	200	205	PF00069	0.441
MOD_CDK_SPxK_1	61	67	PF00069	0.479
MOD_CK1_1	146	152	PF00069	0.681
MOD_CK1_1	81	87	PF00069	0.788
MOD_CK2_1	204	210	PF00069	0.434
MOD_CK2_1	249	255	PF00069	0.488
MOD_CK2_1	281	287	PF00069	0.465
MOD_GlcNHglycan	158	161	PF01048	0.792
MOD_GlcNHglycan	200	203	PF01048	0.348
MOD_GlcNHglycan	243	247	PF01048	0.485
MOD_GlcNHglycan	33	36	PF01048	0.446
MOD_GlcNHglycan	331	334	PF01048	0.253
MOD_GlcNHglycan	83	86	PF01048	0.731
MOD_GSK3_1	139	146	PF00069	0.639
MOD_GSK3_1	200	207	PF00069	0.418
MOD_GSK3_1	329	336	PF00069	0.501
MOD_NEK2_1	130	135	PF00069	0.448
MOD_NEK2_1	143	148	PF00069	0.721
MOD_NEK2_2	204	209	PF00069	0.517
MOD_NEK2_2	68	73	PF00069	0.363
MOD_PKA_1	139	145	PF00069	0.614
MOD_PKA_1	221	227	PF00069	0.461
MOD_PKA_2	130	136	PF00069	0.495
MOD_PKA_2	139	145	PF00069	0.717
MOD_PKA_2	318	324	PF00069	0.440
MOD_Plk_2-3	210	216	PF00069	0.404
MOD_Plk_4	143	149	PF00069	0.764
MOD_Plk_4	15	21	PF00069	0.359
MOD_Plk_4	222	228	PF00069	0.436
MOD_Plk_4	318	324	PF00069	0.440
MOD_Plk_4	333	339	PF00069	0.534
MOD_ProDKin_1	200	206	PF00069	0.442
MOD_ProDKin_1	281	287	PF00069	0.475
MOD_ProDKin_1	61	67	PF00069	0.451
MOD_SUMO_for_1	90	93	PF00179	0.796
MOD_SUMO_rev_2	2	10	PF00179	0.405
MOD_SUMO_rev_2	289	297	PF00179	0.527
TRG_DiLeu_BaEn_2	286	292	PF01217	0.496
TRG_ENDOCYTIC_2	314	317	PF00928	0.440
TRG_ENDOCYTIC_2	338	341	PF00928	0.440
TRG_ER_diArg_1	115	117	PF00400	0.568
TRG_ER_diArg_1	138	140	PF00400	0.560
TRG_ER_diArg_1	86	88	PF00400	0.670
TRG_NLS_MonoExtN_4	217	224	PF00514	0.486

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P8I4	Leptomonas seymouri	68%	100%
A0A1X0P763	Trypanosomatidae	44%	100%
A0A3R7KXJ2	Trypanosoma rangeli	45%	100%
A0A3S5H734	Leishmania donovani	94%	100%
A4H9J0	Leishmania braziliensis	78%	100%
A4HXV3	Leishmania infantum	94%	100%
C9ZZV0	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	33%	100%
E9ARL6	Leishmania mexicana (strain MHOM/GT/2001/U1103)	94%	100%
V5B6J4	Trypanosoma cruzi	44%	100%