Proline--tRNA ligase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

bifunctional aminoacyl-tRNA synthetase, putative

Species:

Leishmania major

UniProt:

Q4QDS0_LEIMA

TriTrypDb:

LmjF.18.1220 , LMJLV39_180017800 , LMJSD75_180018100

Length:

731

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 3
Silverman et al.	no	yes: 0
Pissara et al.	yes	yes: 28

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 6

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 15
NetGPI	no	yes: 0, no: 15

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	16
GO:0017101	aminoacyl-tRNA synthetase multienzyme complex	3	3
GO:0032991	protein-containing complex	1	3
GO:0110165	cellular anatomical entity	1	16
GO:0140535	intracellular protein-containing complex	2	3
GO:1902494	catalytic complex	2	3
GO:0016020	membrane	2	1

Expansion

Sequence features

Q4QDS0

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4QDS0

PDB structure(s): 5xil_A

Function

Biological processes
Term	Name	Level	Count
GO:0006082	organic acid metabolic process	3	16
GO:0006139	nucleobase-containing compound metabolic process	3	16
GO:0006399	tRNA metabolic process	7	16
GO:0006418	tRNA aminoacylation for protein translation	6	16
GO:0006433	prolyl-tRNA aminoacylation	7	16
GO:0006520	amino acid metabolic process	3	16
GO:0006725	cellular aromatic compound metabolic process	3	16
GO:0006807	nitrogen compound metabolic process	2	16
GO:0008152	metabolic process	1	16
GO:0009987	cellular process	1	16
GO:0016070	RNA metabolic process	5	16
GO:0019752	carboxylic acid metabolic process	5	16
GO:0034641	cellular nitrogen compound metabolic process	3	16
GO:0034660	ncRNA metabolic process	6	16
GO:0043038	amino acid activation	4	16
GO:0043039	tRNA aminoacylation	5	16
GO:0043170	macromolecule metabolic process	3	16
GO:0043436	oxoacid metabolic process	4	16
GO:0044237	cellular metabolic process	2	16
GO:0044238	primary metabolic process	2	16
GO:0044281	small molecule metabolic process	2	16
GO:0046483	heterocycle metabolic process	3	16
GO:0071704	organic substance metabolic process	2	16
GO:0090304	nucleic acid metabolic process	4	16
GO:1901360	organic cyclic compound metabolic process	3	16
GO:1901564	organonitrogen compound metabolic process	3	16

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	16
GO:0002161	aminoacyl-tRNA editing activity	5	15
GO:0003824	catalytic activity	1	16
GO:0004812	aminoacyl-tRNA ligase activity	4	16
GO:0004827	proline-tRNA ligase activity	5	16
GO:0005488	binding	1	16
GO:0005524	ATP binding	5	16
GO:0016787	hydrolase activity	2	15
GO:0016788	hydrolase activity, acting on ester bonds	3	15
GO:0016874	ligase activity	2	16
GO:0016875	ligase activity, forming carbon-oxygen bonds	3	16
GO:0017076	purine nucleotide binding	4	16
GO:0030554	adenyl nucleotide binding	5	16
GO:0032553	ribonucleotide binding	3	16
GO:0032555	purine ribonucleotide binding	4	16
GO:0032559	adenyl ribonucleotide binding	5	16
GO:0035639	purine ribonucleoside triphosphate binding	4	16
GO:0036094	small molecule binding	2	16
GO:0043167	ion binding	2	16
GO:0043168	anion binding	3	16
GO:0052689	carboxylic ester hydrolase activity	4	15
GO:0097159	organic cyclic compound binding	2	16
GO:0097367	carbohydrate derivative binding	2	16
GO:0140098	catalytic activity, acting on RNA	3	16
GO:0140101	catalytic activity, acting on a tRNA	4	16
GO:0140640	catalytic activity, acting on a nucleic acid	2	16
GO:1901265	nucleoside phosphate binding	3	16
GO:1901363	heterocyclic compound binding	2	16

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	552	556	PF00656	0.500
CLV_NRD_NRD_1	416	418	PF00675	0.375
CLV_NRD_NRD_1	647	649	PF00675	0.341
CLV_PCSK_KEX2_1	201	203	PF00082	0.766
CLV_PCSK_KEX2_1	227	229	PF00082	0.441
CLV_PCSK_PC1ET2_1	201	203	PF00082	0.766
CLV_PCSK_PC1ET2_1	227	229	PF00082	0.441
CLV_PCSK_SKI1_1	127	131	PF00082	0.318
CLV_PCSK_SKI1_1	373	377	PF00082	0.288
CLV_PCSK_SKI1_1	392	396	PF00082	0.336
CLV_PCSK_SKI1_1	52	56	PF00082	0.300
CLV_PCSK_SKI1_1	719	723	PF00082	0.319
DEG_COP1_1	637	646	PF00400	0.401
DOC_CKS1_1	44	49	PF01111	0.513
DOC_CYCLIN_yCln2_LP_2	320	326	PF00134	0.690
DOC_MAPK_gen_1	127	136	PF00069	0.571
DOC_MAPK_gen_1	142	151	PF00069	0.494
DOC_MAPK_gen_1	340	350	PF00069	0.488
DOC_MAPK_gen_1	57	66	PF00069	0.457
DOC_MAPK_MEF2A_6	118	125	PF00069	0.571
DOC_MAPK_MEF2A_6	343	352	PF00069	0.488
DOC_MAPK_MEF2A_6	486	494	PF00069	0.513
DOC_MAPK_MEF2A_6	569	577	PF00069	0.488
DOC_PP1_RVXF_1	164	171	PF00149	0.478
DOC_PP1_RVXF_1	84	91	PF00149	0.513
DOC_PP2B_LxvP_1	409	412	PF13499	0.488
DOC_PP2B_LxvP_1	696	699	PF13499	0.488
DOC_PP4_FxxP_1	363	366	PF00568	0.488
DOC_PP4_FxxP_1	44	47	PF00568	0.470
DOC_PP4_FxxP_1	464	467	PF00568	0.488
DOC_PP4_FxxP_1	700	703	PF00568	0.488
DOC_USP7_MATH_1	583	587	PF00917	0.592
DOC_USP7_MATH_1	596	600	PF00917	0.527
DOC_USP7_MATH_1	671	675	PF00917	0.488
DOC_USP7_MATH_2	92	98	PF00917	0.456
DOC_USP7_UBL2_3	715	719	PF12436	0.541
DOC_WW_Pin1_4	105	110	PF00397	0.500
DOC_WW_Pin1_4	129	134	PF00397	0.542
DOC_WW_Pin1_4	364	369	PF00397	0.500
DOC_WW_Pin1_4	43	48	PF00397	0.476
DOC_WW_Pin1_4	555	560	PF00397	0.479
DOC_WW_Pin1_4	603	608	PF00397	0.528
LIG_14-3-3_CanoR_1	327	336	PF00244	0.488
LIG_14-3-3_CanoR_1	415	424	PF00244	0.539
LIG_14-3-3_CanoR_1	506	516	PF00244	0.375
LIG_14-3-3_CanoR_1	597	601	PF00244	0.492
LIG_14-3-3_CanoR_1	633	643	PF00244	0.532
LIG_Actin_WH2_2	117	132	PF00022	0.549
LIG_APCC_ABBA_1	492	497	PF00400	0.421
LIG_BIR_II_1	1	5	PF00653	0.645
LIG_Clathr_ClatBox_1	148	152	PF01394	0.474
LIG_deltaCOP1_diTrp_1	374	384	PF00928	0.500
LIG_deltaCOP1_diTrp_1	638	642	PF00928	0.373
LIG_EH1_1	118	126	PF00400	0.403
LIG_FHA_1	120	126	PF00498	0.554
LIG_FHA_1	365	371	PF00498	0.500
LIG_FHA_1	428	434	PF00498	0.488
LIG_FHA_1	44	50	PF00498	0.510
LIG_FHA_1	483	489	PF00498	0.481
LIG_FHA_1	508	514	PF00498	0.462
LIG_FHA_1	629	635	PF00498	0.456
LIG_FHA_1	638	644	PF00498	0.323
LIG_FHA_1	693	699	PF00498	0.529
LIG_FHA_1	720	726	PF00498	0.575
LIG_FHA_1	83	89	PF00498	0.546
LIG_FHA_2	239	245	PF00498	0.368
LIG_FHA_2	31	37	PF00498	0.500
LIG_FHA_2	316	322	PF00498	0.725
LIG_FHA_2	417	423	PF00498	0.485
LIG_FHA_2	429	435	PF00498	0.495
LIG_FHA_2	521	527	PF00498	0.489
LIG_FHA_2	680	686	PF00498	0.770
LIG_FHA_2	70	76	PF00498	0.549
LIG_LIR_Apic_2	362	366	PF02991	0.488
LIG_LIR_Apic_2	461	467	PF02991	0.518
LIG_LIR_Gen_1	108	116	PF02991	0.534
LIG_LIR_Gen_1	233	243	PF02991	0.399
LIG_LIR_Gen_1	399	409	PF02991	0.500
LIG_LIR_Gen_1	637	646	PF02991	0.340
LIG_LIR_Gen_1	722	731	PF02991	0.551
LIG_LIR_LC3C_4	146	150	PF02991	0.456
LIG_LIR_Nem_3	108	113	PF02991	0.546
LIG_LIR_Nem_3	233	239	PF02991	0.396
LIG_LIR_Nem_3	247	251	PF02991	0.365
LIG_LIR_Nem_3	283	289	PF02991	0.404
LIG_LIR_Nem_3	332	338	PF02991	0.488
LIG_LIR_Nem_3	399	404	PF02991	0.489
LIG_LIR_Nem_3	637	642	PF02991	0.340
LIG_LIR_Nem_3	722	727	PF02991	0.551
LIG_NRBOX	11	17	PF00104	0.358
LIG_Pex14_2	456	460	PF04695	0.488
LIG_SH2_CRK	401	405	PF00017	0.488
LIG_SH2_NCK_1	401	405	PF00017	0.500
LIG_SH2_PTP2	236	239	PF00017	0.438
LIG_SH2_SRC	236	239	PF00017	0.438
LIG_SH2_STAP1	163	167	PF00017	0.508
LIG_SH2_STAP1	338	342	PF00017	0.488
LIG_SH2_STAP1	401	405	PF00017	0.484
LIG_SH2_STAP1	429	433	PF00017	0.488
LIG_SH2_STAT5	236	239	PF00017	0.380
LIG_SH2_STAT5	255	258	PF00017	0.275
LIG_SH2_STAT5	263	266	PF00017	0.354
LIG_SH2_STAT5	285	288	PF00017	0.400
LIG_SH2_STAT5	429	432	PF00017	0.488
LIG_SH3_3	134	140	PF00018	0.470
LIG_SH3_3	330	336	PF00018	0.500
LIG_SH3_3	499	505	PF00018	0.399
LIG_SH3_3	700	706	PF00018	0.510
LIG_SUMO_SIM_anti_2	11	18	PF11976	0.452
LIG_SUMO_SIM_anti_2	393	399	PF11976	0.549
LIG_SUMO_SIM_anti_2	509	516	PF11976	0.460
LIG_SUMO_SIM_anti_2	62	67	PF11976	0.458
LIG_SUMO_SIM_anti_2	97	104	PF11976	0.563
LIG_SUMO_SIM_par_1	121	126	PF11976	0.570
LIG_SUMO_SIM_par_1	393	399	PF11976	0.549
LIG_SUMO_SIM_par_1	62	67	PF11976	0.472
LIG_TRAF2_1	33	36	PF00917	0.528
LIG_TRAF2_1	523	526	PF00917	0.531
LIG_TRAF2_1	527	530	PF00917	0.521
LIG_TRAF2_1	670	673	PF00917	0.493
LIG_TRFH_1	136	140	PF08558	0.474
LIG_TYR_ITIM	246	251	PF00017	0.411
LIG_UBA3_1	112	118	PF00899	0.538
LIG_UBA3_1	12	17	PF00899	0.519
LIG_WRC_WIRS_1	433	438	PF05994	0.590
LIG_WW_3	503	507	PF00397	0.398
MOD_CDK_SPxxK_3	364	371	PF00069	0.500
MOD_CDK_SPxxK_3	555	562	PF00069	0.488
MOD_CK1_1	214	220	PF00069	0.678
MOD_CK1_1	364	370	PF00069	0.500
MOD_CK2_1	238	244	PF00069	0.374
MOD_CK2_1	24	30	PF00069	0.482
MOD_CK2_1	290	296	PF00069	0.667
MOD_CK2_1	315	321	PF00069	0.706
MOD_CK2_1	400	406	PF00069	0.563
MOD_CK2_1	416	422	PF00069	0.428
MOD_CK2_1	428	434	PF00069	0.495
MOD_CK2_1	520	526	PF00069	0.489
MOD_CK2_1	667	673	PF00069	0.488
MOD_Cter_Amidation	415	418	PF01082	0.300
MOD_GlcNHglycan	1	4	PF01048	0.509
MOD_GlcNHglycan	173	176	PF01048	0.587
MOD_GlcNHglycan	213	216	PF01048	0.704
MOD_GlcNHglycan	232	235	PF01048	0.276
MOD_GlcNHglycan	250	254	PF01048	0.261
MOD_GlcNHglycan	593	596	PF01048	0.367
MOD_GlcNHglycan	669	672	PF01048	0.288
MOD_GSK3_1	428	435	PF00069	0.511
MOD_GSK3_1	477	484	PF00069	0.479
MOD_GSK3_1	517	524	PF00069	0.512
MOD_GSK3_1	64	71	PF00069	0.503
MOD_GSK3_1	667	674	PF00069	0.488
MOD_GSK3_1	715	722	PF00069	0.575
MOD_N-GLC_1	119	124	PF02516	0.332
MOD_N-GLC_1	230	235	PF02516	0.484
MOD_N-GLC_1	555	560	PF02516	0.282
MOD_NEK2_1	288	293	PF00069	0.677
MOD_NEK2_1	531	536	PF00069	0.592
MOD_NEK2_1	55	60	PF00069	0.505
MOD_NEK2_2	400	405	PF00069	0.546
MOD_PIKK_1	507	513	PF00454	0.504
MOD_PKA_1	667	673	PF00069	0.488
MOD_PKA_2	326	332	PF00069	0.488
MOD_PKA_2	416	422	PF00069	0.552
MOD_PKA_2	591	597	PF00069	0.503
MOD_PKA_2	634	640	PF00069	0.511
MOD_Plk_1	119	125	PF00069	0.555
MOD_Plk_1	143	149	PF00069	0.537
MOD_Plk_1	230	236	PF00069	0.461
MOD_Plk_1	470	476	PF00069	0.529
MOD_Plk_1	583	589	PF00069	0.590
MOD_Plk_2-3	30	36	PF00069	0.513
MOD_Plk_4	119	125	PF00069	0.556
MOD_Plk_4	144	150	PF00069	0.503
MOD_Plk_4	329	335	PF00069	0.494
MOD_Plk_4	400	406	PF00069	0.546
MOD_Plk_4	432	438	PF00069	0.500
MOD_Plk_4	470	476	PF00069	0.505
MOD_Plk_4	720	726	PF00069	0.575
MOD_ProDKin_1	105	111	PF00069	0.500
MOD_ProDKin_1	129	135	PF00069	0.542
MOD_ProDKin_1	364	370	PF00069	0.500
MOD_ProDKin_1	43	49	PF00069	0.476
MOD_ProDKin_1	555	561	PF00069	0.479
MOD_ProDKin_1	603	609	PF00069	0.528
MOD_SUMO_for_1	226	229	PF00179	0.428
MOD_SUMO_for_1	385	388	PF00179	0.575
MOD_SUMO_rev_2	214	222	PF00179	0.597
MOD_SUMO_rev_2	669	677	PF00179	0.475
TRG_DiLeu_BaEn_1	11	16	PF01217	0.505
TRG_DiLeu_BaEn_2	637	643	PF01217	0.462
TRG_ENDOCYTIC_2	165	168	PF00928	0.420
TRG_ENDOCYTIC_2	236	239	PF00928	0.367
TRG_ENDOCYTIC_2	248	251	PF00928	0.413
TRG_ENDOCYTIC_2	285	288	PF00928	0.401
TRG_ENDOCYTIC_2	401	404	PF00928	0.479
TRG_ENDOCYTIC_2	405	408	PF00928	0.479
TRG_ER_diArg_1	370	373	PF00400	0.488
TRG_ER_diArg_1	414	417	PF00400	0.581
TRG_ER_diArg_1	547	550	PF00400	0.579
TRG_ER_diArg_1	633	636	PF00400	0.574
TRG_NES_CRM1_1	11	22	PF08389	0.434
TRG_Pf-PMV_PEXEL_1	39	43	PF00026	0.203
TRG_Pf-PMV_PEXEL_1	580	584	PF00026	0.369

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1HY85	Leptomonas seymouri	82%	100%
A0A0S4JN58	Bodo saltans	59%	89%
A0A1X0P793	Trypanosomatidae	67%	90%
A0A3R7KPX0	Trypanosoma rangeli	68%	85%
A0A3S7WV15	Leishmania donovani	96%	100%
A0A3S7WV32	Leishmania donovani	87%	100%
A0M4Z3	Gramella forsetii (strain KT0803)	35%	100%
A4H9N1	Leishmania braziliensis	80%	100%
A4HXZ5	Leishmania infantum	87%	90%
A4HY00	Leishmania infantum	96%	100%
A5FA31	Flavobacterium johnsoniae (strain ATCC 17061 / DSM 2064 / JCM 8514 / NBRC 14942 / NCIMB 11054 / UW101)	35%	100%
A6L5I2	Phocaeicola vulgatus (strain ATCC 8482 / DSM 1447 / JCM 5826 / CCUG 4940 / NBRC 14291 / NCTC 11154)	37%	100%
A6LAP3	Parabacteroides distasonis (strain ATCC 8503 / DSM 20701 / CIP 104284 / JCM 5825 / NCTC 11152)	35%	100%
B0R3A3	Halobacterium salinarum (strain ATCC 29341 / DSM 671 / R1)	39%	100%
B2RJG1	Porphyromonas gingivalis (strain ATCC 33277 / DSM 20709 / CIP 103683 / JCM 12257 / NCTC 11834 / 2561)	37%	100%
B3ER64	Amoebophilus asiaticus (strain 5a2)	37%	100%
B7J1X2	Borreliella burgdorferi (strain ZS7)	37%	100%
B9LUM0	Halorubrum lacusprofundi (strain ATCC 49239 / DSM 5036 / JCM 8891 / ACAM 34)	41%	100%
D0A5B2	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	65%	90%
E9ARQ8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	84%	100%
E9ARQ9	Leishmania mexicana (strain MHOM/GT/2001/U1103)	94%	100%
O51363	Borreliella burgdorferi (strain ATCC 35210 / DSM 4680 / CIP 102532 / B31)	37%	100%
O60155	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	45%	100%
P38708	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	47%	100%
Q0SNA5	Borreliella afzelii (strain PKo)	36%	100%
Q11Q45	Cytophaga hutchinsonii (strain ATCC 33406 / DSM 1761 / CIP 103989 / NBRC 15051 / NCIMB 9469 / D465)	34%	100%
Q18JG3	Haloquadratum walsbyi (strain DSM 16790 / HBSQ001)	40%	100%
Q1GTS7	Sphingopyxis alaskensis (strain DSM 13593 / LMG 18877 / RB2256)	36%	100%
Q1J0W2	Deinococcus geothermalis (strain DSM 11300 / AG-3a)	35%	100%
Q2ILX2	Anaeromyxobacter dehalogenans (strain 2CP-C)	39%	100%
Q2RZJ2	Salinibacter ruber (strain DSM 13855 / M31)	35%	100%
Q2W136	Magnetospirillum magneticum (strain AMB-1 / ATCC 700264)	36%	100%
Q4QDS1	Leishmania major	89%	100%
Q5LCF0	Bacteroides fragilis (strain ATCC 25285 / DSM 2151 / CCUG 4856 / JCM 11019 / NCTC 9343 / Onslow)	37%	100%
Q64TJ6	Bacteroides fragilis (strain YCH46)	37%	100%
Q661L6	Borrelia garinii subsp. bavariensis (strain ATCC BAA-2496 / DSM 23469 / PBi)	37%	100%
Q67PA6	Symbiobacterium thermophilum (strain T / IAM 14863)	41%	100%
Q6MIW2	Bdellovibrio bacteriovorus (strain ATCC 15356 / DSM 50701 / NCIMB 9529 / HD100)	36%	100%
Q7MVS7	Porphyromonas gingivalis (strain ATCC BAA-308 / W83)	37%	100%
Q7UGJ7	Rhodopirellula baltica (strain DSM 10527 / NCIMB 13988 / SH1)	35%	100%
Q7X2P1	Sphingomonas elodea	35%	100%
Q81Z76	Bacillus anthracis	41%	100%
Q8A988	Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / JCM 5827 / CCUG 10774 / NCTC 10582 / VPI-5482 / E50)	37%	100%
Q8I5R7	Plasmodium falciparum (isolate 3D7)	40%	98%
Q8SSD7	Encephalitozoon cuniculi (strain GB-M1)	49%	100%
Q9FYR6	Arabidopsis thaliana	37%	100%
Q9HS52	Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1)	39%	100%
Q9M1R2	Arabidopsis thaliana	54%	100%
Q9RUW4	Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422)	36%	100%
V5AZ66	Trypanosoma cruzi	67%	90%