Glutamate--cysteine ligase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

gamma-glutamylcysteine synthetase, putative

Species:

Leishmania major

UniProt:

Q4QDM2_LEIMA

TriTrypDb:

LmjF.18.1660 , LMJLV39_180022500 , LMJSD75_180022700

Length:

687

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005654	nucleoplasm	2	2
GO:0110165	cellular anatomical entity	1	2

Expansion

Sequence features

Q4QDM2

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4QDM2

Function

Biological processes
Term	Name	Level	Count
GO:0006518	peptide metabolic process	4	12
GO:0006575	cellular modified amino acid metabolic process	3	12
GO:0006749	glutathione metabolic process	4	12
GO:0006750	glutathione biosynthetic process	5	12
GO:0006790	sulfur compound metabolic process	3	12
GO:0006807	nitrogen compound metabolic process	2	12
GO:0008152	metabolic process	1	12
GO:0009058	biosynthetic process	2	12
GO:0009987	cellular process	1	12
GO:0019184	nonribosomal peptide biosynthetic process	6	12
GO:0034641	cellular nitrogen compound metabolic process	3	12
GO:0042398	cellular modified amino acid biosynthetic process	4	12
GO:0043043	peptide biosynthetic process	5	12
GO:0043603	amide metabolic process	3	12
GO:0043604	amide biosynthetic process	4	12
GO:0044237	cellular metabolic process	2	12
GO:0044249	cellular biosynthetic process	3	12
GO:0044271	cellular nitrogen compound biosynthetic process	4	12
GO:0044272	sulfur compound biosynthetic process	4	12
GO:0071704	organic substance metabolic process	2	12
GO:1901564	organonitrogen compound metabolic process	3	12
GO:1901566	organonitrogen compound biosynthetic process	4	12
GO:1901576	organic substance biosynthetic process	3	12

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	12
GO:0003824	catalytic activity	1	12
GO:0004357	glutamate-cysteine ligase activity	5	12
GO:0005488	binding	1	12
GO:0005524	ATP binding	5	12
GO:0016874	ligase activity	2	12
GO:0016879	ligase activity, forming carbon-nitrogen bonds	3	12
GO:0016881	acid-amino acid ligase activity	4	12
GO:0017076	purine nucleotide binding	4	12
GO:0030554	adenyl nucleotide binding	5	12
GO:0032553	ribonucleotide binding	3	12
GO:0032555	purine ribonucleotide binding	4	12
GO:0032559	adenyl ribonucleotide binding	5	12
GO:0035639	purine ribonucleoside triphosphate binding	4	12
GO:0036094	small molecule binding	2	12
GO:0043167	ion binding	2	12
GO:0043168	anion binding	3	12
GO:0097159	organic cyclic compound binding	2	12
GO:0097367	carbohydrate derivative binding	2	12
GO:1901265	nucleoside phosphate binding	3	12
GO:1901363	heterocyclic compound binding	2	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	111	115	PF00656	0.445
CLV_C14_Caspase3-7	413	417	PF00656	0.380
CLV_NRD_NRD_1	122	124	PF00675	0.348
CLV_NRD_NRD_1	189	191	PF00675	0.304
CLV_NRD_NRD_1	230	232	PF00675	0.673
CLV_NRD_NRD_1	384	386	PF00675	0.261
CLV_NRD_NRD_1	556	558	PF00675	0.253
CLV_NRD_NRD_1	631	633	PF00675	0.338
CLV_PCSK_FUR_1	187	191	PF00082	0.304
CLV_PCSK_KEX2_1	122	124	PF00082	0.350
CLV_PCSK_KEX2_1	189	191	PF00082	0.304
CLV_PCSK_KEX2_1	230	232	PF00082	0.611
CLV_PCSK_KEX2_1	384	386	PF00082	0.304
CLV_PCSK_KEX2_1	604	606	PF00082	0.302
CLV_PCSK_PC1ET2_1	604	606	PF00082	0.448
CLV_PCSK_SKI1_1	224	228	PF00082	0.598
CLV_PCSK_SKI1_1	235	239	PF00082	0.565
CLV_PCSK_SKI1_1	271	275	PF00082	0.501
CLV_PCSK_SKI1_1	364	368	PF00082	0.243
CLV_PCSK_SKI1_1	373	377	PF00082	0.243
CLV_PCSK_SKI1_1	558	562	PF00082	0.243
CLV_PCSK_SKI1_1	633	637	PF00082	0.337
DEG_COP1_1	162	170	PF00400	0.323
DEG_SPOP_SBC_1	253	257	PF00917	0.528
DOC_CKS1_1	273	278	PF01111	0.329
DOC_CKS1_1	503	508	PF01111	0.243
DOC_CKS1_1	624	629	PF01111	0.270
DOC_CYCLIN_RxL_1	268	275	PF00134	0.528
DOC_CYCLIN_RxL_1	361	371	PF00134	0.337
DOC_CYCLIN_yCln2_LP_2	273	279	PF00134	0.373
DOC_MAPK_DCC_7	193	202	PF00069	0.370
DOC_MAPK_gen_1	187	196	PF00069	0.302
DOC_MAPK_gen_1	38	48	PF00069	0.470
DOC_MAPK_gen_1	394	402	PF00069	0.277
DOC_MAPK_GRA24_9	186	202	PF00069	0.375
DOC_MAPK_HePTP_8	186	198	PF00069	0.298
DOC_MAPK_MEF2A_6	189	198	PF00069	0.299
DOC_MAPK_MEF2A_6	396	404	PF00069	0.277
DOC_MAPK_MEF2A_6	430	438	PF00069	0.355
DOC_PP1_RVXF_1	492	498	PF00149	0.243
DOC_PP2B_LxvP_1	165	168	PF13499	0.323
DOC_PP2B_LxvP_1	251	254	PF13499	0.506
DOC_PP4_FxxP_1	482	485	PF00568	0.337
DOC_USP7_MATH_1	254	258	PF00917	0.583
DOC_USP7_MATH_1	355	359	PF00917	0.243
DOC_USP7_MATH_1	573	577	PF00917	0.270
DOC_WW_Pin1_4	156	161	PF00397	0.363
DOC_WW_Pin1_4	217	222	PF00397	0.442
DOC_WW_Pin1_4	272	277	PF00397	0.331
DOC_WW_Pin1_4	357	362	PF00397	0.243
DOC_WW_Pin1_4	502	507	PF00397	0.243
DOC_WW_Pin1_4	603	608	PF00397	0.386
DOC_WW_Pin1_4	623	628	PF00397	0.270
DOC_WW_Pin1_4	98	103	PF00397	0.448
LIG_14-3-3_CanoR_1	235	242	PF00244	0.545
LIG_14-3-3_CanoR_1	632	641	PF00244	0.340
LIG_Actin_WH2_2	175	191	PF00022	0.321
LIG_APCC_ABBA_1	198	203	PF00400	0.299
LIG_APCC_ABBA_1	59	64	PF00400	0.279
LIG_deltaCOP1_diTrp_1	490	497	PF00928	0.302
LIG_eIF4E_1	589	595	PF01652	0.258
LIG_FHA_1	105	111	PF00498	0.324
LIG_FHA_1	136	142	PF00498	0.306
LIG_FHA_1	181	187	PF00498	0.294
LIG_FHA_1	236	242	PF00498	0.632
LIG_FHA_1	265	271	PF00498	0.466
LIG_FHA_1	328	334	PF00498	0.258
LIG_FHA_1	406	412	PF00498	0.314
LIG_FHA_1	518	524	PF00498	0.277
LIG_FHA_1	63	69	PF00498	0.476
LIG_FHA_1	646	652	PF00498	0.255
LIG_FHA_2	204	210	PF00498	0.475
LIG_FHA_2	462	468	PF00498	0.264
LIG_FHA_2	503	509	PF00498	0.243
LIG_FHA_2	564	570	PF00498	0.302
LIG_FHA_2	635	641	PF00498	0.424
LIG_FHA_2	679	685	PF00498	0.372
LIG_GBD_Chelix_1	651	659	PF00786	0.270
LIG_HCF-1_HBM_1	103	106	PF13415	0.376
LIG_Integrin_isoDGR_2	233	235	PF01839	0.531
LIG_LIR_Apic_2	479	485	PF02991	0.386
LIG_LIR_Gen_1	138	146	PF02991	0.481
LIG_LIR_Gen_1	371	381	PF02991	0.243
LIG_LIR_Gen_1	395	404	PF02991	0.251
LIG_LIR_Gen_1	424	434	PF02991	0.307
LIG_LIR_Gen_1	474	485	PF02991	0.243
LIG_LIR_Gen_1	611	620	PF02991	0.246
LIG_LIR_Nem_3	138	143	PF02991	0.488
LIG_LIR_Nem_3	371	377	PF02991	0.243
LIG_LIR_Nem_3	395	400	PF02991	0.251
LIG_LIR_Nem_3	424	429	PF02991	0.282
LIG_LIR_Nem_3	466	472	PF02991	0.243
LIG_LIR_Nem_3	474	480	PF02991	0.242
LIG_LIR_Nem_3	585	591	PF02991	0.257
LIG_LIR_Nem_3	611	615	PF02991	0.246
LIG_LIR_Nem_3	669	675	PF02991	0.366
LIG_LIR_Nem_3	92	96	PF02991	0.330
LIG_Pex14_1	493	497	PF04695	0.243
LIG_Pex14_1	89	93	PF04695	0.333
LIG_Pex14_2	33	37	PF04695	0.423
LIG_PTB_Apo_2	506	513	PF02174	0.243
LIG_PTB_Apo_2	87	94	PF02174	0.377
LIG_PTB_Phospho_1	87	93	PF10480	0.379
LIG_SH2_CRK	124	128	PF00017	0.405
LIG_SH2_CRK	343	347	PF00017	0.243
LIG_SH2_CRK	397	401	PF00017	0.277
LIG_SH2_CRK	531	535	PF00017	0.295
LIG_SH2_CRK	589	593	PF00017	0.258
LIG_SH2_NCK_1	397	401	PF00017	0.290
LIG_SH2_NCK_1	531	535	PF00017	0.319
LIG_SH2_SRC	201	204	PF00017	0.259
LIG_SH2_SRC	672	675	PF00017	0.368
LIG_SH2_STAP1	106	110	PF00017	0.313
LIG_SH2_STAP1	161	165	PF00017	0.382
LIG_SH2_STAP1	282	286	PF00017	0.345
LIG_SH2_STAP1	343	347	PF00017	0.302
LIG_SH2_STAP1	454	458	PF00017	0.277
LIG_SH2_STAT3	281	284	PF00017	0.312
LIG_SH2_STAT5	106	109	PF00017	0.295
LIG_SH2_STAT5	201	204	PF00017	0.299
LIG_SH2_STAT5	285	288	PF00017	0.429
LIG_SH2_STAT5	531	534	PF00017	0.245
LIG_SH2_STAT5	554	557	PF00017	0.243
LIG_SH2_STAT5	598	601	PF00017	0.347
LIG_SH2_STAT5	612	615	PF00017	0.146
LIG_SH2_STAT5	672	675	PF00017	0.394
LIG_SH3_3	138	144	PF00018	0.291
LIG_SH3_3	305	311	PF00018	0.334
LIG_SH3_3	511	517	PF00018	0.337
LIG_SH3_3	85	91	PF00018	0.386
LIG_SH3_3	99	105	PF00018	0.281
LIG_SH3_5	260	264	PF00018	0.697
LIG_SUMO_SIM_anti_2	344	350	PF11976	0.337
LIG_SUMO_SIM_anti_2	532	539	PF11976	0.337
LIG_SUMO_SIM_par_1	106	112	PF11976	0.299
LIG_SUMO_SIM_par_1	303	309	PF11976	0.349
LIG_SUMO_SIM_par_1	352	358	PF11976	0.174
LIG_SUMO_SIM_par_1	455	464	PF11976	0.253
LIG_SUMO_SIM_par_1	63	71	PF11976	0.484
LIG_TRAF2_1	568	571	PF00917	0.270
LIG_TRFH_1	589	593	PF08558	0.258
LIG_WRC_WIRS_1	93	98	PF05994	0.324
MOD_CDK_SPxxK_3	217	224	PF00069	0.468
MOD_CDK_SPxxK_3	357	364	PF00069	0.243
MOD_CK1_1	109	115	PF00069	0.355
MOD_CK1_1	155	161	PF00069	0.379
MOD_CK1_1	357	363	PF00069	0.237
MOD_CK1_1	405	411	PF00069	0.314
MOD_CK1_1	645	651	PF00069	0.265
MOD_CK2_1	226	232	PF00069	0.553
MOD_CK2_1	421	427	PF00069	0.314
MOD_CK2_1	461	467	PF00069	0.254
MOD_CK2_1	502	508	PF00069	0.243
MOD_CK2_1	565	571	PF00069	0.300
MOD_CK2_1	573	579	PF00069	0.214
MOD_CK2_1	678	684	PF00069	0.381
MOD_Cter_Amidation	602	605	PF01082	0.270
MOD_GlcNHglycan	246	250	PF01048	0.670
MOD_GlcNHglycan	324	327	PF01048	0.319
MOD_GlcNHglycan	357	360	PF01048	0.316
MOD_GlcNHglycan	431	434	PF01048	0.280
MOD_GlcNHglycan	620	623	PF01048	0.337
MOD_GSK3_1	12	19	PF00069	0.439
MOD_GSK3_1	135	142	PF00069	0.309
MOD_GSK3_1	152	159	PF00069	0.486
MOD_GSK3_1	203	210	PF00069	0.346
MOD_GSK3_1	392	399	PF00069	0.243
MOD_GSK3_1	472	479	PF00069	0.258
MOD_GSK3_1	565	572	PF00069	0.272
MOD_GSK3_1	614	621	PF00069	0.301
MOD_GSK3_1	62	69	PF00069	0.423
MOD_GSK3_1	678	685	PF00069	0.514
MOD_N-GLC_1	172	177	PF02516	0.338
MOD_N-GLC_1	180	185	PF02516	0.283
MOD_N-GLC_1	414	419	PF02516	0.286
MOD_N-GLC_1	421	426	PF02516	0.267
MOD_N-GLC_2	299	301	PF02516	0.442
MOD_NEK2_1	3	8	PF00069	0.429
MOD_NEK2_1	322	327	PF00069	0.258
MOD_NEK2_1	354	359	PF00069	0.243
MOD_NEK2_1	414	419	PF00069	0.302
MOD_NEK2_1	429	434	PF00069	0.194
MOD_NEK2_1	608	613	PF00069	0.342
MOD_NEK2_1	618	623	PF00069	0.282
MOD_NEK2_1	634	639	PF00069	0.230
MOD_NEK2_1	647	652	PF00069	0.280
MOD_NEK2_1	678	683	PF00069	0.491
MOD_NEK2_1	97	102	PF00069	0.329
MOD_NEK2_2	161	166	PF00069	0.354
MOD_NMyristoyl	1	7	PF02799	0.449
MOD_PIKK_1	159	165	PF00454	0.394
MOD_PIKK_1	414	420	PF00454	0.246
MOD_PK_1	396	402	PF00069	0.277
MOD_PKA_2	573	579	PF00069	0.338
MOD_PKA_2	618	624	PF00069	0.302
MOD_PKA_2	645	651	PF00069	0.274
MOD_Plk_1	414	420	PF00069	0.291
MOD_Plk_1	421	427	PF00069	0.263
MOD_Plk_1	62	68	PF00069	0.416
MOD_Plk_2-3	203	209	PF00069	0.369
MOD_Plk_4	136	142	PF00069	0.314
MOD_Plk_4	161	167	PF00069	0.351
MOD_Plk_4	303	309	PF00069	0.505
MOD_Plk_4	608	614	PF00069	0.352
MOD_Plk_4	92	98	PF00069	0.328
MOD_ProDKin_1	156	162	PF00069	0.355
MOD_ProDKin_1	217	223	PF00069	0.439
MOD_ProDKin_1	272	278	PF00069	0.325
MOD_ProDKin_1	357	363	PF00069	0.243
MOD_ProDKin_1	502	508	PF00069	0.243
MOD_ProDKin_1	603	609	PF00069	0.386
MOD_ProDKin_1	623	629	PF00069	0.270
MOD_ProDKin_1	98	104	PF00069	0.458
MOD_SUMO_rev_2	600	606	PF00179	0.306
TRG_DiLeu_BaEn_2	91	97	PF01217	0.330
TRG_DiLeu_BaEn_3	387	393	PF01217	0.222
TRG_DiLeu_BaLyEn_6	514	519	PF01217	0.337
TRG_ENDOCYTIC_2	124	127	PF00928	0.341
TRG_ENDOCYTIC_2	343	346	PF00928	0.243
TRG_ENDOCYTIC_2	397	400	PF00928	0.251
TRG_ENDOCYTIC_2	425	428	PF00928	0.278
TRG_ENDOCYTIC_2	454	457	PF00928	0.277
TRG_ENDOCYTIC_2	531	534	PF00928	0.293
TRG_ENDOCYTIC_2	589	592	PF00928	0.257
TRG_ENDOCYTIC_2	612	615	PF00928	0.243
TRG_ENDOCYTIC_2	672	675	PF00928	0.516
TRG_ENDOCYTIC_2	93	96	PF00928	0.325
TRG_ER_diArg_1	122	124	PF00400	0.353
TRG_ER_diArg_1	186	189	PF00400	0.289
TRG_ER_diArg_1	384	386	PF00400	0.378
TRG_ER_diArg_1	79	82	PF00400	0.367
TRG_Pf-PMV_PEXEL_1	597	601	PF00026	0.302
TRG_Pf-PMV_PEXEL_1	653	657	PF00026	0.319

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1P9N5	Leptomonas seymouri	73%	100%
A0A0S4IM75	Bodo saltans	53%	100%
A0A1X0P757	Trypanosomatidae	58%	100%
A0A3S5IRL4	Trypanosoma rangeli	59%	100%
A0A3S7WV51	Leishmania donovani	95%	100%
A4H9R8	Leishmania braziliensis	77%	100%
A4HY37	Leishmania infantum	94%	100%
D0A548	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	59%	100%
E9ARV1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	89%	100%
P32477	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	31%	100%
Q09768	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	34%	100%
V5BDE4	Trypanosoma cruzi	60%	100%