Vacuolar protein-sorting-associated protein 36

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Vacuolar protein sorting protein 36 Vps36/EAP30/Vps36 family, putative

Species:

Leishmania major

UniProt:

Q4QDE7_LEIMA

TriTrypDb:

LmjF.19.0740 , LMJLV39_190013000 , LMJSD75_190012700

Length:

446

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 3
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0000814	ESCRT II complex	3	11
GO:0005737	cytoplasm	2	2
GO:0010008	endosome membrane	5	11
GO:0012506	vesicle membrane	4	11
GO:0016020	membrane	2	11
GO:0030659	cytoplasmic vesicle membrane	5	11
GO:0031090	organelle membrane	3	11
GO:0031902	late endosome membrane	6	11
GO:0032991	protein-containing complex	1	11
GO:0036452	ESCRT complex	2	11
GO:0098588	bounding membrane of organelle	4	11
GO:0098796	membrane protein complex	2	11
GO:0110165	cellular anatomical entity	1	11

Expansion

Sequence features

Q4QDE7

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4QDE7

Function

Biological processes
Term	Name	Level	Count
GO:0006810	transport	3	11
GO:0006886	intracellular protein transport	4	11
GO:0007034	vacuolar transport	4	11
GO:0008104	protein localization	4	11
GO:0009987	cellular process	1	11
GO:0015031	protein transport	4	11
GO:0016192	vesicle-mediated transport	4	11
GO:0016197	endosomal transport	4	11
GO:0032509	endosome transport via multivesicular body sorting pathway	5	11
GO:0032511	late endosome to vacuole transport via multivesicular body sorting pathway	6	11
GO:0033036	macromolecule localization	2	11
GO:0033365	protein localization to organelle	5	11
GO:0043328	protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway	5	11
GO:0045184	establishment of protein localization	3	11
GO:0045324	late endosome to vacuole transport	5	11
GO:0046907	intracellular transport	3	11
GO:0051179	localization	1	11
GO:0051234	establishment of localization	2	11
GO:0051641	cellular localization	2	11
GO:0051649	establishment of localization in cell	3	11
GO:0070727	cellular macromolecule localization	3	11
GO:0071702	organic substance transport	4	11
GO:0071705	nitrogen compound transport	4	11
GO:0071985	multivesicular body sorting pathway	5	11
GO:0072594	establishment of protein localization to organelle	4	11
GO:0072665	protein localization to vacuole	6	11
GO:0072666	establishment of protein localization to vacuole	5	11

Molecular functions
Term	Name	Level	Count
GO:0005488	binding	1	11
GO:0005515	protein binding	2	11
GO:0005543	phospholipid binding	3	11
GO:0008289	lipid binding	2	11
GO:0032182	ubiquitin-like protein binding	3	11
GO:0032266	phosphatidylinositol-3-phosphate binding	6	11
GO:0035091	phosphatidylinositol binding	4	11
GO:0043130	ubiquitin binding	4	11
GO:1901981	phosphatidylinositol phosphate binding	5	11

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	200	202	PF00675	0.361
CLV_NRD_NRD_1	326	328	PF00675	0.293
CLV_NRD_NRD_1	69	71	PF00675	0.337
CLV_PCSK_KEX2_1	200	202	PF00082	0.361
CLV_PCSK_KEX2_1	69	71	PF00082	0.337
CLV_PCSK_SKI1_1	327	331	PF00082	0.255
CLV_PCSK_SKI1_1	393	397	PF00082	0.344
CLV_Separin_Metazoa	66	70	PF03568	0.537
DEG_Nend_UBRbox_2	1	3	PF02207	0.545
DEG_SPOP_SBC_1	144	148	PF00917	0.625
DEG_SPOP_SBC_1	382	386	PF00917	0.497
DOC_CYCLIN_yCln2_LP_2	296	302	PF00134	0.537
DOC_CYCLIN_yCln2_LP_2	61	67	PF00134	0.561
DOC_MAPK_DCC_7	292	302	PF00069	0.537
DOC_MAPK_gen_1	34	44	PF00069	0.544
DOC_MAPK_gen_1	393	403	PF00069	0.533
DOC_MAPK_MEF2A_6	251	260	PF00069	0.537
DOC_MAPK_MEF2A_6	95	103	PF00069	0.469
DOC_PP1_RVXF_1	98	104	PF00149	0.482
DOC_PP2B_LxvP_1	61	64	PF13499	0.472
DOC_USP7_MATH_1	131	135	PF00917	0.679
DOC_USP7_MATH_1	176	180	PF00917	0.532
DOC_USP7_MATH_1	211	215	PF00917	0.561
DOC_USP7_MATH_1	311	315	PF00917	0.506
DOC_USP7_MATH_1	389	393	PF00917	0.489
DOC_USP7_MATH_1	83	87	PF00917	0.478
DOC_WW_Pin1_4	140	145	PF00397	0.750
DOC_WW_Pin1_4	295	300	PF00397	0.488
DOC_WW_Pin1_4	353	358	PF00397	0.502
LIG_14-3-3_CanoR_1	136	144	PF00244	0.537
LIG_14-3-3_CanoR_1	381	389	PF00244	0.546
LIG_BRCT_BRCA1_1	269	273	PF00533	0.561
LIG_BRCT_BRCA1_1	77	81	PF00533	0.561
LIG_CtBP_PxDLS_1	64	68	PF00389	0.561
LIG_deltaCOP1_diTrp_1	2	6	PF00928	0.519
LIG_FHA_1	145	151	PF00498	0.718
LIG_FHA_1	231	237	PF00498	0.452
LIG_FHA_1	341	347	PF00498	0.537
LIG_FHA_1	438	444	PF00498	0.490
LIG_FHA_1	44	50	PF00498	0.458
LIG_FHA_2	125	131	PF00498	0.632
LIG_FHA_2	179	185	PF00498	0.475
LIG_FHA_2	218	224	PF00498	0.487
LIG_FHA_2	25	31	PF00498	0.526
LIG_FHA_2	382	388	PF00498	0.561
LIG_FHA_2	430	436	PF00498	0.515
LIG_GBD_Chelix_1	413	421	PF00786	0.271
LIG_LIR_Gen_1	109	119	PF02991	0.459
LIG_LIR_Gen_1	270	281	PF02991	0.561
LIG_LIR_Gen_1	315	324	PF02991	0.538
LIG_LIR_Gen_1	440	446	PF02991	0.443
LIG_LIR_Nem_3	109	115	PF02991	0.464
LIG_LIR_Nem_3	250	256	PF02991	0.394
LIG_LIR_Nem_3	283	288	PF02991	0.458
LIG_LIR_Nem_3	315	321	PF02991	0.475
LIG_LIR_Nem_3	431	437	PF02991	0.441
LIG_LIR_Nem_3	440	444	PF02991	0.382
LIG_LIR_Nem_3	78	84	PF02991	0.531
LIG_PDZ_Class_2	441	446	PF00595	0.385
LIG_RPA_C_Fungi	328	340	PF08784	0.439
LIG_SH2_CRK	112	116	PF00017	0.404
LIG_SH2_CRK	318	322	PF00017	0.422
LIG_SH2_NCK_1	318	322	PF00017	0.422
LIG_SH2_PTP2	98	101	PF00017	0.472
LIG_SH2_STAP1	112	116	PF00017	0.520
LIG_SH2_STAP1	439	443	PF00017	0.494
LIG_SH2_STAT5	426	429	PF00017	0.340
LIG_SH2_STAT5	437	440	PF00017	0.423
LIG_SH2_STAT5	98	101	PF00017	0.399
LIG_SH3_1	154	160	PF00018	0.657
LIG_SH3_3	152	158	PF00018	0.676
LIG_SH3_3	274	280	PF00018	0.311
LIG_SH3_3	318	324	PF00018	0.300
LIG_SH3_3	342	348	PF00018	0.432
LIG_SH3_3	88	94	PF00018	0.330
LIG_SH3_CIN85_PxpxPR_1	156	161	PF14604	0.610
LIG_SUMO_SIM_anti_2	298	304	PF11976	0.455
LIG_SUMO_SIM_anti_2	416	422	PF11976	0.455
LIG_SUMO_SIM_par_1	319	325	PF11976	0.418
LIG_UBA3_1	284	290	PF00899	0.300
LIG_UBA3_1	417	424	PF00899	0.422
LIG_UBA3_1	89	95	PF00899	0.222
MOD_CK1_1	102	108	PF00069	0.483
MOD_CK1_1	140	146	PF00069	0.780
MOD_CK1_1	172	178	PF00069	0.422
MOD_CK1_1	229	235	PF00069	0.380
MOD_CK1_1	245	251	PF00069	0.204
MOD_CK1_1	353	359	PF00069	0.403
MOD_CK1_1	383	389	PF00069	0.284
MOD_CK1_1	399	405	PF00069	0.397
MOD_CK1_1	43	49	PF00069	0.445
MOD_CK1_1	76	82	PF00069	0.368
MOD_CK2_1	124	130	PF00069	0.416
MOD_CK2_1	217	223	PF00069	0.368
MOD_CK2_1	399	405	PF00069	0.398
MOD_Cter_Amidation	325	328	PF01082	0.422
MOD_GlcNHglycan	104	107	PF01048	0.463
MOD_GlcNHglycan	133	136	PF01048	0.696
MOD_GlcNHglycan	139	142	PF01048	0.663
MOD_GlcNHglycan	174	177	PF01048	0.329
MOD_GlcNHglycan	324	327	PF01048	0.268
MOD_GlcNHglycan	372	375	PF01048	0.372
MOD_GlcNHglycan	70	73	PF01048	0.335
MOD_GlcNHglycan	78	81	PF01048	0.299
MOD_GlcNHglycan	85	88	PF01048	0.284
MOD_GSK3_1	120	127	PF00069	0.405
MOD_GSK3_1	131	138	PF00069	0.720
MOD_GSK3_1	140	147	PF00069	0.760
MOD_GSK3_1	172	179	PF00069	0.358
MOD_GSK3_1	211	218	PF00069	0.375
MOD_GSK3_1	222	229	PF00069	0.270
MOD_GSK3_1	238	245	PF00069	0.256
MOD_GSK3_1	360	367	PF00069	0.383
MOD_GSK3_1	370	377	PF00069	0.375
MOD_N-GLC_1	242	247	PF02516	0.472
MOD_N-GLC_2	304	306	PF02516	0.400
MOD_NEK2_1	178	183	PF00069	0.350
MOD_NEK2_1	313	318	PF00069	0.435
MOD_NEK2_1	350	355	PF00069	0.366
MOD_NEK2_1	42	47	PF00069	0.330
MOD_PIKK_1	32	38	PF00454	0.396
MOD_PIKK_1	364	370	PF00454	0.292
MOD_PKA_1	120	126	PF00069	0.343
MOD_PKA_2	135	141	PF00069	0.530
MOD_PKA_2	223	229	PF00069	0.440
MOD_PKA_2	380	386	PF00069	0.377
MOD_PKA_2	68	74	PF00069	0.455
MOD_PKB_1	201	209	PF00069	0.378
MOD_Plk_1	222	228	PF00069	0.321
MOD_Plk_1	230	236	PF00069	0.300
MOD_Plk_1	333	339	PF00069	0.384
MOD_Plk_1	374	380	PF00069	0.394
MOD_Plk_1	399	405	PF00069	0.446
MOD_Plk_1	55	61	PF00069	0.455
MOD_Plk_2-3	223	229	PF00069	0.340
MOD_Plk_2-3	394	400	PF00069	0.378
MOD_Plk_4	120	126	PF00069	0.343
MOD_Plk_4	24	30	PF00069	0.455
MOD_Plk_4	259	265	PF00069	0.307
MOD_Plk_4	304	310	PF00069	0.413
MOD_Plk_4	333	339	PF00069	0.314
MOD_Plk_4	341	347	PF00069	0.330
MOD_Plk_4	375	381	PF00069	0.376
MOD_Plk_4	416	422	PF00069	0.409
MOD_Plk_4	56	62	PF00069	0.355
MOD_ProDKin_1	140	146	PF00069	0.753
MOD_ProDKin_1	295	301	PF00069	0.354
MOD_ProDKin_1	353	359	PF00069	0.373
MOD_SUMO_rev_2	2	8	PF00179	0.378
MOD_SUMO_rev_2	392	398	PF00179	0.455
TRG_DiLeu_BaLyEn_6	277	282	PF01217	0.313
TRG_ENDOCYTIC_2	112	115	PF00928	0.439
TRG_ENDOCYTIC_2	253	256	PF00928	0.222
TRG_ENDOCYTIC_2	318	321	PF00928	0.422
TRG_ENDOCYTIC_2	98	101	PF00928	0.402
TRG_ER_diArg_1	199	201	PF00400	0.455
TRG_ER_diArg_1	68	70	PF00400	0.422
TRG_NES_CRM1_1	394	405	PF08389	0.455
TRG_NES_CRM1_1	56	66	PF08389	0.378
TRG_Pf-PMV_PEXEL_1	200	204	PF00026	0.369
TRG_Pf-PMV_PEXEL_1	254	259	PF00026	0.378

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P3P0	Leptomonas seymouri	64%	98%
A0A1X0P698	Trypanosomatidae	44%	99%
A0A3R7M0G6	Trypanosoma rangeli	40%	96%
A0A3S7WVL4	Leishmania donovani	94%	100%
A4HA31	Leishmania braziliensis	75%	96%
A4HY94	Leishmania infantum	94%	100%
A5PK00	Bos taurus	29%	100%
D0A0M8	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	36%	99%
E9AS26	Leishmania mexicana (strain MHOM/GT/2001/U1103)	90%	100%
P0C0A2	Rattus norvegicus	29%	100%
Q6DDF4	Xenopus laevis	28%	100%
Q7ZVK4	Danio rerio	28%	100%
Q86VN1	Homo sapiens	29%	100%
Q91XD6	Mus musculus	29%	100%
Q9VU87	Drosophila melanogaster	24%	100%
V5AMS4	Trypanosoma cruzi	42%	98%