tRNA pseudouridine synthase A-like protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

tRNA pseudouridine synthase A-like protein

Species:

Leishmania major

UniProt:

Q4QDB3_LEIMA

TriTrypDb:

LmjF.19.1020 , LMJLV39_190015800 , LMJSD75_190015700

Length:

525

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 9

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Could not find GO cellular_component term for this entry.

Expansion

Sequence features

Q4QDB3

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4QDB3

Function

Biological processes
Term	Name	Level	Count
GO:0001522	pseudouridine synthesis	6	7
GO:0006139	nucleobase-containing compound metabolic process	3	11
GO:0006396	RNA processing	6	11
GO:0006399	tRNA metabolic process	7	11
GO:0006400	tRNA modification	6	2
GO:0006725	cellular aromatic compound metabolic process	3	11
GO:0006807	nitrogen compound metabolic process	2	11
GO:0008033	tRNA processing	8	11
GO:0008152	metabolic process	1	11
GO:0009451	RNA modification	5	7
GO:0009987	cellular process	1	11
GO:0016070	RNA metabolic process	5	11
GO:0031119	tRNA pseudouridine synthesis	7	2
GO:0034470	ncRNA processing	7	11
GO:0034641	cellular nitrogen compound metabolic process	3	11
GO:0034660	ncRNA metabolic process	6	11
GO:0043170	macromolecule metabolic process	3	11
GO:0043412	macromolecule modification	4	7
GO:0044237	cellular metabolic process	2	11
GO:0044238	primary metabolic process	2	11
GO:0046483	heterocycle metabolic process	3	11
GO:0071704	organic substance metabolic process	2	11
GO:0090304	nucleic acid metabolic process	4	11
GO:1901360	organic cyclic compound metabolic process	3	11

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	11
GO:0003723	RNA binding	4	11
GO:0003824	catalytic activity	1	11
GO:0004730	pseudouridylate synthase activity	5	6
GO:0005488	binding	1	11
GO:0009982	pseudouridine synthase activity	4	11
GO:0016829	lyase activity	2	6
GO:0016835	carbon-oxygen lyase activity	3	6
GO:0016836	hydro-lyase activity	4	6
GO:0016853	isomerase activity	2	11
GO:0016866	intramolecular transferase activity	3	11
GO:0097159	organic cyclic compound binding	2	11
GO:1901363	heterocyclic compound binding	2	11
GO:0106029	tRNA pseudouridine synthase activity	5	1
GO:0140098	catalytic activity, acting on RNA	3	1
GO:0140101	catalytic activity, acting on a tRNA	4	1
GO:0140640	catalytic activity, acting on a nucleic acid	2	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	62	66	PF00656	0.578
CLV_NRD_NRD_1	19	21	PF00675	0.656
CLV_NRD_NRD_1	213	215	PF00675	0.338
CLV_NRD_NRD_1	243	245	PF00675	0.540
CLV_NRD_NRD_1	268	270	PF00675	0.328
CLV_NRD_NRD_1	327	329	PF00675	0.383
CLV_NRD_NRD_1	360	362	PF00675	0.570
CLV_NRD_NRD_1	475	477	PF00675	0.318
CLV_PCSK_KEX2_1	213	215	PF00082	0.327
CLV_PCSK_KEX2_1	243	245	PF00082	0.379
CLV_PCSK_KEX2_1	268	270	PF00082	0.328
CLV_PCSK_KEX2_1	327	329	PF00082	0.383
CLV_PCSK_KEX2_1	360	362	PF00082	0.570
CLV_PCSK_KEX2_1	480	482	PF00082	0.296
CLV_PCSK_PC1ET2_1	480	482	PF00082	0.287
CLV_PCSK_PC7_1	476	482	PF00082	0.287
CLV_PCSK_SKI1_1	182	186	PF00082	0.318
CLV_PCSK_SKI1_1	213	217	PF00082	0.456
CLV_PCSK_SKI1_1	230	234	PF00082	0.504
CLV_PCSK_SKI1_1	33	37	PF00082	0.564
CLV_PCSK_SKI1_1	394	398	PF00082	0.484
CLV_PCSK_SKI1_1	477	481	PF00082	0.347
CLV_Separin_Metazoa	422	426	PF03568	0.347
DEG_APCC_DBOX_1	243	251	PF00400	0.405
DEG_APCC_DBOX_1	408	416	PF00400	0.402
DEG_Nend_Nbox_1	1	3	PF02207	0.594
DEG_SPOP_SBC_1	330	334	PF00917	0.508
DOC_CDC14_PxL_1	90	98	PF14671	0.544
DOC_MAPK_gen_1	213	221	PF00069	0.343
DOC_MAPK_gen_1	243	249	PF00069	0.413
DOC_MAPK_MEF2A_6	425	434	PF00069	0.269
DOC_PP2B_LxvP_1	193	196	PF13499	0.410
DOC_PP2B_LxvP_1	296	299	PF13499	0.411
DOC_USP7_MATH_1	196	200	PF00917	0.382
DOC_USP7_MATH_1	262	266	PF00917	0.340
DOC_USP7_MATH_1	329	333	PF00917	0.492
DOC_USP7_MATH_1	341	345	PF00917	0.456
DOC_USP7_MATH_1	353	357	PF00917	0.692
DOC_USP7_MATH_1	467	471	PF00917	0.269
DOC_USP7_MATH_1	6	10	PF00917	0.663
DOC_WW_Pin1_4	482	487	PF00397	0.290
LIG_14-3-3_CanoR_1	213	222	PF00244	0.461
LIG_14-3-3_CanoR_1	235	239	PF00244	0.368
LIG_14-3-3_CanoR_1	33	42	PF00244	0.469
LIG_14-3-3_CanoR_1	394	399	PF00244	0.485
LIG_14-3-3_CanoR_1	445	451	PF00244	0.197
LIG_Actin_WH2_2	437	454	PF00022	0.287
LIG_APCC_ABBA_1	507	512	PF00400	0.522
LIG_BIR_III_2	159	163	PF00653	0.591
LIG_BIR_III_2	512	516	PF00653	0.536
LIG_BRCT_BRCA1_1	176	180	PF00533	0.479
LIG_BRCT_BRCA1_2	176	182	PF00533	0.407
LIG_EH1_1	441	449	PF00400	0.287
LIG_FHA_1	106	112	PF00498	0.747
LIG_FHA_1	195	201	PF00498	0.442
LIG_FHA_1	214	220	PF00498	0.425
LIG_FHA_1	235	241	PF00498	0.391
LIG_FHA_2	385	391	PF00498	0.397
LIG_FHA_2	491	497	PF00498	0.269
LIG_FHA_2	74	80	PF00498	0.526
LIG_LIR_Gen_1	177	188	PF02991	0.405
LIG_LIR_Gen_1	293	301	PF02991	0.481
LIG_LIR_Nem_3	145	151	PF02991	0.524
LIG_LIR_Nem_3	177	183	PF02991	0.428
LIG_LIR_Nem_3	293	298	PF02991	0.457
LIG_LIR_Nem_3	311	317	PF02991	0.236
LIG_LIR_Nem_3	387	391	PF02991	0.381
LIG_LIR_Nem_3	505	510	PF02991	0.379
LIG_LIR_Nem_3	87	93	PF02991	0.439
LIG_LYPXL_yS_3	93	96	PF13949	0.556
LIG_MAD2	274	282	PF02301	0.321
LIG_Pex14_1	495	499	PF04695	0.269
LIG_Pex14_2	468	472	PF04695	0.383
LIG_SH2_CRK	148	152	PF00017	0.568
LIG_SH2_CRK	273	277	PF00017	0.297
LIG_SH2_NCK_1	516	520	PF00017	0.481
LIG_SH2_SRC	119	122	PF00017	0.472
LIG_SH2_SRC	295	298	PF00017	0.469
LIG_SH2_SRC	510	513	PF00017	0.443
LIG_SH2_SRC	516	519	PF00017	0.467
LIG_SH2_STAT3	499	502	PF00017	0.361
LIG_SH2_STAT5	119	122	PF00017	0.472
LIG_SH2_STAT5	25	28	PF00017	0.605
LIG_SH2_STAT5	271	274	PF00017	0.295
LIG_SH2_STAT5	295	298	PF00017	0.469
LIG_SH2_STAT5	510	513	PF00017	0.420
LIG_SH3_3	122	128	PF00018	0.624
LIG_SH3_3	216	222	PF00018	0.372
LIG_SH3_3	362	368	PF00018	0.617
LIG_SH3_3	494	500	PF00018	0.334
LIG_SH3_3	512	518	PF00018	0.412
LIG_SUMO_SIM_anti_2	197	202	PF11976	0.462
LIG_SUMO_SIM_anti_2	419	425	PF11976	0.269
LIG_SUMO_SIM_par_1	245	251	PF11976	0.332
LIG_SUMO_SIM_par_1	517	523	PF11976	0.515
LIG_TYR_ITIM	91	96	PF00017	0.533
LIG_WRC_WIRS_1	385	390	PF05994	0.377
MOD_CK1_1	23	29	PF00069	0.634
MOD_CK1_1	333	339	PF00069	0.615
MOD_CK1_1	345	351	PF00069	0.523
MOD_CK1_1	37	43	PF00069	0.655
MOD_CK1_1	58	64	PF00069	0.724
MOD_CK2_1	109	115	PF00069	0.630
MOD_CK2_1	220	226	PF00069	0.416
MOD_CK2_1	374	380	PF00069	0.427
MOD_CK2_1	384	390	PF00069	0.355
MOD_CK2_1	467	473	PF00069	0.269
MOD_GlcNHglycan	112	115	PF01048	0.751
MOD_GlcNHglycan	264	267	PF01048	0.330
MOD_GlcNHglycan	335	338	PF01048	0.610
MOD_GlcNHglycan	344	347	PF01048	0.568
MOD_GlcNHglycan	39	42	PF01048	0.639
MOD_GlcNHglycan	4	7	PF01048	0.624
MOD_GlcNHglycan	469	472	PF01048	0.283
MOD_GlcNHglycan	57	60	PF01048	0.621
MOD_GSK3_1	105	112	PF00069	0.603
MOD_GSK3_1	147	154	PF00069	0.572
MOD_GSK3_1	16	23	PF00069	0.543
MOD_GSK3_1	2	9	PF00069	0.629
MOD_GSK3_1	329	336	PF00069	0.532
MOD_GSK3_1	341	348	PF00069	0.474
MOD_GSK3_1	407	414	PF00069	0.380
MOD_N-GLC_1	55	60	PF02516	0.657
MOD_NEK2_1	16	21	PF00069	0.615
MOD_NEK2_1	2	7	PF00069	0.577
MOD_NEK2_1	317	322	PF00069	0.397
MOD_NEK2_1	34	39	PF00069	0.563
MOD_NEK2_1	472	477	PF00069	0.402
MOD_NEK2_1	72	77	PF00069	0.576
MOD_NEK2_2	248	253	PF00069	0.464
MOD_PIKK_1	374	380	PF00454	0.520
MOD_PKA_1	20	26	PF00069	0.552
MOD_PKA_1	213	219	PF00069	0.454
MOD_PKA_2	213	219	PF00069	0.454
MOD_PKA_2	234	240	PF00069	0.365
MOD_PKA_2	262	268	PF00069	0.328
MOD_PKA_2	6	12	PF00069	0.596
MOD_Plk_2-3	220	226	PF00069	0.506
MOD_Plk_4	196	202	PF00069	0.421
MOD_Plk_4	234	240	PF00069	0.365
MOD_Plk_4	435	441	PF00069	0.278
MOD_Plk_4	446	452	PF00069	0.252
MOD_Plk_4	490	496	PF00069	0.269
MOD_ProDKin_1	482	488	PF00069	0.290
MOD_SUMO_for_1	479	482	PF00179	0.287
MOD_SUMO_rev_2	313	323	PF00179	0.330
TRG_DiLeu_BaEn_1	427	432	PF01217	0.402
TRG_ENDOCYTIC_2	148	151	PF00928	0.555
TRG_ENDOCYTIC_2	273	276	PF00928	0.297
TRG_ENDOCYTIC_2	295	298	PF00928	0.469
TRG_ENDOCYTIC_2	314	317	PF00928	0.448
TRG_ENDOCYTIC_2	516	519	PF00928	0.476
TRG_ENDOCYTIC_2	93	96	PF00928	0.556
TRG_ER_diArg_1	212	214	PF00400	0.324
TRG_ER_diArg_1	267	269	PF00400	0.351
TRG_ER_diArg_1	326	328	PF00400	0.368
TRG_ER_diArg_1	359	361	PF00400	0.555
TRG_NLS_MonoExtC_3	475	480	PF00514	0.331
TRG_NLS_MonoExtN_4	476	481	PF00514	0.331
TRG_Pf-PMV_PEXEL_1	20	24	PF00026	0.520
TRG_Pf-PMV_PEXEL_1	230	234	PF00026	0.362
TRG_Pf-PMV_PEXEL_1	374	378	PF00026	0.469

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I702	Leptomonas seymouri	72%	79%
A0A1X0P663	Trypanosomatidae	61%	84%
A0A3S7WVG1	Leishmania donovani	96%	100%
A0A422NHE5	Trypanosoma rangeli	62%	86%
A4HA54	Leishmania braziliensis	86%	100%
A4HYB9	Leishmania infantum	95%	100%
D0A0P7	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	59%	89%
E9AS53	Leishmania mexicana (strain MHOM/GT/2001/U1103)	94%	100%
V5BNI6	Trypanosoma cruzi	64%	86%