ZZ-type domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

E3 ubiquitin-protein ligase KCMF1, putative

Species:

Leishmania major

UniProt:

Q4QD14_LEIMA

TriTrypDb:

LmjF.20.0290 , LMJLV39_200008100 , LMJSD75_200008000

Length:

799

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 9
NetGPI	no	yes: 0, no: 9

Cellular components
Term	Name	Level	Count
GO:0005622	intracellular anatomical structure	2	2
GO:0005737	cytoplasm	2	2
GO:0005773	vacuole	5	2
GO:0005776	autophagosome	6	2
GO:0016234	inclusion body	3	2
GO:0016235	aggresome	4	2
GO:0031974	membrane-enclosed lumen	2	2
GO:0031981	nuclear lumen	5	2
GO:0032838	plasma membrane bounded cell projection cytoplasm	4	2
GO:0043226	organelle	2	2
GO:0043227	membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043231	intracellular membrane-bounded organelle	4	2
GO:0043233	organelle lumen	3	2
GO:0044753	amphisome	7	2
GO:0070013	intracellular organelle lumen	4	2
GO:0097014	ciliary plasm	5	2
GO:0099568	cytoplasmic region	3	2
GO:0110165	cellular anatomical entity	1	2

Expansion

Sequence features

Q4QD14

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4QD14

Function

Biological processes
Term	Name	Level	Count
GO:0000422	autophagy of mitochondrion	4	2
GO:0000423	mitophagy	5	2
GO:0006914	autophagy	3	2
GO:0006996	organelle organization	4	2
GO:0007005	mitochondrion organization	5	2
GO:0007032	endosome organization	6	2
GO:0008152	metabolic process	1	2
GO:0009056	catabolic process	2	2
GO:0009987	cellular process	1	2
GO:0016043	cellular component organization	3	2
GO:0016050	vesicle organization	5	2
GO:0016236	macroautophagy	4	2
GO:0022411	cellular component disassembly	4	2
GO:0035973	aggrephagy	6	2
GO:0044237	cellular metabolic process	2	2
GO:0044248	cellular catabolic process	3	2
GO:0061726	mitochondrion disassembly	6	2
GO:0061912	selective autophagy	5	2
GO:0061919	process utilizing autophagic mechanism	2	2
GO:0071840	cellular component organization or biogenesis	2	2
GO:1903008	organelle disassembly	5	2

Molecular functions
Term	Name	Level	Count
GO:0005080	protein kinase C binding	6	2
GO:0005488	binding	1	10
GO:0005515	protein binding	2	2
GO:0008270	zinc ion binding	6	10
GO:0019899	enzyme binding	3	2
GO:0019900	kinase binding	4	2
GO:0019901	protein kinase binding	5	2
GO:0031593	polyubiquitin modification-dependent protein binding	4	2
GO:0043167	ion binding	2	10
GO:0043169	cation binding	3	10
GO:0046872	metal ion binding	4	10
GO:0046914	transition metal ion binding	5	10
GO:0070530	K63-linked polyubiquitin modification-dependent protein binding	5	2
GO:0140030	modification-dependent protein binding	3	2

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	229	233	PF00656	0.569
CLV_C14_Caspase3-7	472	476	PF00656	0.627
CLV_MEL_PAP_1	75	81	PF00089	0.306
CLV_NRD_NRD_1	155	157	PF00675	0.488
CLV_NRD_NRD_1	460	462	PF00675	0.784
CLV_NRD_NRD_1	500	502	PF00675	0.560
CLV_NRD_NRD_1	556	558	PF00675	0.570
CLV_NRD_NRD_1	71	73	PF00675	0.517
CLV_PCSK_FUR_1	458	462	PF00082	0.789
CLV_PCSK_KEX2_1	341	343	PF00082	0.474
CLV_PCSK_KEX2_1	37	39	PF00082	0.563
CLV_PCSK_KEX2_1	458	460	PF00082	0.782
CLV_PCSK_KEX2_1	500	502	PF00082	0.483
CLV_PCSK_KEX2_1	556	558	PF00082	0.570
CLV_PCSK_KEX2_1	71	73	PF00082	0.575
CLV_PCSK_KEX2_1	721	723	PF00082	0.550
CLV_PCSK_PC1ET2_1	341	343	PF00082	0.440
CLV_PCSK_PC1ET2_1	37	39	PF00082	0.563
CLV_PCSK_PC1ET2_1	721	723	PF00082	0.597
CLV_PCSK_PC7_1	717	723	PF00082	0.557
CLV_PCSK_SKI1_1	156	160	PF00082	0.460
CLV_PCSK_SKI1_1	281	285	PF00082	0.480
CLV_PCSK_SKI1_1	71	75	PF00082	0.494
CLV_PCSK_SKI1_1	84	88	PF00082	0.336
DEG_APCC_DBOX_1	250	258	PF00400	0.514
DEG_Nend_Nbox_1	1	3	PF02207	0.527
DEG_SPOP_SBC_1	170	174	PF00917	0.588
DEG_SPOP_SBC_1	66	70	PF00917	0.479
DOC_CKS1_1	123	128	PF01111	0.580
DOC_CKS1_1	583	588	PF01111	0.496
DOC_CKS1_1	752	757	PF01111	0.672
DOC_CYCLIN_RxL_1	278	287	PF00134	0.526
DOC_CYCLIN_RxL_1	495	507	PF00134	0.520
DOC_CYCLIN_yClb1_LxF_4	204	210	PF00134	0.568
DOC_CYCLIN_yCln2_LP_2	120	126	PF00134	0.489
DOC_CYCLIN_yCln2_LP_2	637	643	PF00134	0.432
DOC_CYCLIN_yCln2_LP_2	646	652	PF00134	0.423
DOC_MAPK_gen_1	341	348	PF00069	0.423
DOC_MAPK_gen_1	436	444	PF00069	0.552
DOC_MAPK_gen_1	459	471	PF00069	0.708
DOC_MAPK_gen_1	500	506	PF00069	0.498
DOC_MAPK_MEF2A_6	341	350	PF00069	0.389
DOC_MAPK_MEF2A_6	464	471	PF00069	0.708
DOC_PP2B_LxvP_1	646	649	PF13499	0.487
DOC_PP2B_LxvP_1	770	773	PF13499	0.506
DOC_PP2B_LxvP_1	784	787	PF13499	0.518
DOC_PP4_FxxP_1	783	786	PF00568	0.580
DOC_USP7_MATH_1	170	174	PF00917	0.588
DOC_USP7_MATH_1	231	235	PF00917	0.693
DOC_USP7_MATH_1	519	523	PF00917	0.566
DOC_USP7_MATH_1	576	580	PF00917	0.539
DOC_USP7_MATH_1	66	70	PF00917	0.482
DOC_USP7_MATH_1	678	682	PF00917	0.608
DOC_USP7_MATH_1	759	763	PF00917	0.706
DOC_USP7_UBL2_3	337	341	PF12436	0.592
DOC_WW_Pin1_4	122	127	PF00397	0.520
DOC_WW_Pin1_4	370	375	PF00397	0.453
DOC_WW_Pin1_4	451	456	PF00397	0.670
DOC_WW_Pin1_4	5	10	PF00397	0.624
DOC_WW_Pin1_4	582	587	PF00397	0.473
DOC_WW_Pin1_4	744	749	PF00397	0.675
DOC_WW_Pin1_4	751	756	PF00397	0.652
LIG_14-3-3_CanoR_1	251	255	PF00244	0.433
LIG_14-3-3_CanoR_1	517	525	PF00244	0.531
LIG_14-3-3_CanoR_1	691	696	PF00244	0.425
LIG_14-3-3_CanoR_1	78	82	PF00244	0.513
LIG_Actin_WH2_2	29	47	PF00022	0.567
LIG_AP2alpha_2	309	311	PF02296	0.424
LIG_AP2alpha_2	523	525	PF02296	0.520
LIG_Clathr_ClatBox_1	347	351	PF01394	0.388
LIG_Clathr_ClatBox_1	395	399	PF01394	0.590
LIG_deltaCOP1_diTrp_1	277	284	PF00928	0.515
LIG_deltaCOP1_diTrp_1	309	314	PF00928	0.408
LIG_DLG_GKlike_1	691	699	PF00625	0.447
LIG_FHA_1	105	111	PF00498	0.530
LIG_FHA_1	123	129	PF00498	0.364
LIG_FHA_1	328	334	PF00498	0.694
LIG_FHA_1	439	445	PF00498	0.642
LIG_FHA_1	466	472	PF00498	0.775
LIG_FHA_1	505	511	PF00498	0.428
LIG_FHA_1	592	598	PF00498	0.402
LIG_FHA_1	68	74	PF00498	0.489
LIG_FHA_1	752	758	PF00498	0.727
LIG_FHA_1	787	793	PF00498	0.534
LIG_FHA_2	184	190	PF00498	0.758
LIG_FHA_2	244	250	PF00498	0.538
LIG_FHA_2	357	363	PF00498	0.545
LIG_FHA_2	435	441	PF00498	0.544
LIG_FHA_2	480	486	PF00498	0.498
LIG_FHA_2	542	548	PF00498	0.570
LIG_IBAR_NPY_1	518	520	PF08397	0.630
LIG_Integrin_RGD_1	735	737	PF01839	0.656
LIG_LIR_Apic_2	782	786	PF02991	0.550
LIG_LIR_Gen_1	19	26	PF02991	0.575
LIG_LIR_Gen_1	212	222	PF02991	0.636
LIG_LIR_Gen_1	299	310	PF02991	0.554
LIG_LIR_Gen_1	30	39	PF02991	0.483
LIG_LIR_Gen_1	359	368	PF02991	0.558
LIG_LIR_Gen_1	475	483	PF02991	0.504
LIG_LIR_Gen_1	533	543	PF02991	0.407
LIG_LIR_Nem_3	19	23	PF02991	0.540
LIG_LIR_Nem_3	212	217	PF02991	0.526
LIG_LIR_Nem_3	25	29	PF02991	0.473
LIG_LIR_Nem_3	299	305	PF02991	0.572
LIG_LIR_Nem_3	30	36	PF02991	0.433
LIG_LIR_Nem_3	309	314	PF02991	0.430
LIG_LIR_Nem_3	359	364	PF02991	0.547
LIG_LIR_Nem_3	475	481	PF02991	0.476
LIG_LIR_Nem_3	522	528	PF02991	0.504
LIG_LIR_Nem_3	533	538	PF02991	0.403
LIG_PCNA_PIPBox_1	389	398	PF02747	0.562
LIG_PCNA_yPIPBox_3	389	397	PF02747	0.488
LIG_Pex14_1	136	140	PF04695	0.443
LIG_Pex14_1	268	272	PF04695	0.499
LIG_PTAP_UEV_1	572	577	PF05743	0.491
LIG_SH2_CRK	302	306	PF00017	0.626
LIG_SH2_CRK	76	80	PF00017	0.444
LIG_SH2_PTP2	535	538	PF00017	0.424
LIG_SH2_STAP1	210	214	PF00017	0.644
LIG_SH2_STAP1	302	306	PF00017	0.626
LIG_SH2_STAP1	719	723	PF00017	0.611
LIG_SH2_STAT5	140	143	PF00017	0.549
LIG_SH2_STAT5	514	517	PF00017	0.593
LIG_SH2_STAT5	520	523	PF00017	0.571
LIG_SH2_STAT5	535	538	PF00017	0.293
LIG_SH2_STAT5	542	545	PF00017	0.488
LIG_SH2_STAT5	688	691	PF00017	0.344
LIG_SH2_STAT5	81	84	PF00017	0.533
LIG_SH3_2	323	328	PF14604	0.677
LIG_SH3_3	120	126	PF00018	0.504
LIG_SH3_3	320	326	PF00018	0.531
LIG_SH3_3	570	576	PF00018	0.469
LIG_SH3_3	649	655	PF00018	0.513
LIG_SH3_3	738	744	PF00018	0.735
LIG_SH3_3	747	753	PF00018	0.692
LIG_SUMO_SIM_anti_2	375	380	PF11976	0.456
LIG_SUMO_SIM_par_1	236	242	PF11976	0.522
LIG_SUMO_SIM_par_1	431	438	PF11976	0.536
LIG_SUMO_SIM_par_1	467	473	PF11976	0.770
LIG_TYR_ITIM	74	79	PF00017	0.456
LIG_UBA3_1	335	341	PF00899	0.594
MOD_CDC14_SPxK_1	8	11	PF00782	0.515
MOD_CDK_SPK_2	451	456	PF00069	0.684
MOD_CDK_SPxK_1	5	11	PF00069	0.663
MOD_CDK_SPxxK_3	451	458	PF00069	0.742
MOD_CDK_SPxxK_3	582	589	PF00069	0.557
MOD_CK1_1	127	133	PF00069	0.542
MOD_CK1_1	169	175	PF00069	0.705
MOD_CK1_1	176	182	PF00069	0.670
MOD_CK1_1	413	419	PF00069	0.754
MOD_CK1_1	622	628	PF00069	0.513
MOD_CK1_1	725	731	PF00069	0.646
MOD_CK1_1	746	752	PF00069	0.700
MOD_CK1_1	779	785	PF00069	0.556
MOD_CK2_1	171	177	PF00069	0.725
MOD_CK2_1	243	249	PF00069	0.534
MOD_CK2_1	356	362	PF00069	0.560
MOD_CK2_1	479	485	PF00069	0.503
MOD_CK2_1	566	572	PF00069	0.562
MOD_GlcNHglycan	168	171	PF01048	0.652
MOD_GlcNHglycan	193	196	PF01048	0.761
MOD_GlcNHglycan	200	203	PF01048	0.588
MOD_GlcNHglycan	227	231	PF01048	0.575
MOD_GlcNHglycan	298	301	PF01048	0.574
MOD_GlcNHglycan	39	42	PF01048	0.580
MOD_GlcNHglycan	572	576	PF01048	0.490
MOD_GlcNHglycan	623	627	PF01048	0.441
MOD_GlcNHglycan	778	781	PF01048	0.607
MOD_GSK3_1	1	8	PF00069	0.701
MOD_GSK3_1	166	173	PF00069	0.628
MOD_GSK3_1	183	190	PF00069	0.777
MOD_GSK3_1	243	250	PF00069	0.516
MOD_GSK3_1	387	394	PF00069	0.526
MOD_GSK3_1	412	419	PF00069	0.707
MOD_GSK3_1	434	441	PF00069	0.595
MOD_GSK3_1	465	472	PF00069	0.775
MOD_GSK3_1	591	598	PF00069	0.511
MOD_GSK3_1	618	625	PF00069	0.544
MOD_GSK3_1	723	730	PF00069	0.546
MOD_GSK3_1	742	749	PF00069	0.684
MOD_GSK3_1	759	766	PF00069	0.421
MOD_N-GLC_1	284	289	PF02516	0.450
MOD_N-GLC_1	426	431	PF02516	0.703
MOD_N-GLC_1	479	484	PF02516	0.503
MOD_N-GLC_1	566	571	PF02516	0.556
MOD_N-GLC_2	690	692	PF02516	0.369
MOD_NEK2_1	198	203	PF00069	0.682
MOD_NEK2_1	243	248	PF00069	0.498
MOD_NEK2_1	284	289	PF00069	0.473
MOD_NEK2_1	293	298	PF00069	0.528
MOD_NEK2_1	335	340	PF00069	0.474
MOD_NEK2_1	426	431	PF00069	0.598
MOD_NEK2_1	504	509	PF00069	0.488
MOD_NEK2_1	566	571	PF00069	0.534
MOD_NEK2_1	577	582	PF00069	0.460
MOD_NEK2_1	711	716	PF00069	0.429
MOD_PIKK_1	417	423	PF00454	0.613
MOD_PKA_1	37	43	PF00069	0.582
MOD_PKA_2	166	172	PF00069	0.601
MOD_PKA_2	250	256	PF00069	0.533
MOD_PKA_2	37	43	PF00069	0.582
MOD_PKA_2	413	419	PF00069	0.687
MOD_PKA_2	435	441	PF00069	0.623
MOD_PKA_2	491	497	PF00069	0.498
MOD_PKA_2	696	702	PF00069	0.487
MOD_PKA_2	77	83	PF00069	0.491
MOD_Plk_1	187	193	PF00069	0.679
MOD_Plk_1	284	290	PF00069	0.519
MOD_Plk_1	474	480	PF00069	0.515
MOD_Plk_1	566	572	PF00069	0.562
MOD_Plk_1	622	628	PF00069	0.516
MOD_Plk_4	260	266	PF00069	0.406
MOD_Plk_4	377	383	PF00069	0.488
MOD_Plk_4	391	397	PF00069	0.531
MOD_Plk_4	531	537	PF00069	0.410
MOD_Plk_4	566	572	PF00069	0.513
MOD_Plk_4	779	785	PF00069	0.589
MOD_ProDKin_1	122	128	PF00069	0.529
MOD_ProDKin_1	370	376	PF00069	0.445
MOD_ProDKin_1	451	457	PF00069	0.678
MOD_ProDKin_1	5	11	PF00069	0.625
MOD_ProDKin_1	582	588	PF00069	0.475
MOD_ProDKin_1	744	750	PF00069	0.673
MOD_ProDKin_1	751	757	PF00069	0.651
TRG_DiLeu_BaEn_1	213	218	PF01217	0.606
TRG_DiLeu_BaEn_1	53	58	PF01217	0.473
TRG_DiLeu_BaEn_2	398	404	PF01217	0.580
TRG_DiLeu_BaLyEn_6	655	660	PF01217	0.503
TRG_DiLeu_BaLyEn_6	69	74	PF01217	0.570
TRG_ENDOCYTIC_2	140	143	PF00928	0.434
TRG_ENDOCYTIC_2	26	29	PF00928	0.523
TRG_ENDOCYTIC_2	302	305	PF00928	0.623
TRG_ENDOCYTIC_2	361	364	PF00928	0.505
TRG_ENDOCYTIC_2	535	538	PF00928	0.424
TRG_ENDOCYTIC_2	76	79	PF00928	0.432
TRG_ER_diArg_1	153	156	PF00400	0.458
TRG_ER_diArg_1	457	460	PF00400	0.789
TRG_ER_diArg_1	500	502	PF00400	0.488
TRG_ER_diArg_1	555	557	PF00400	0.572
TRG_ER_diArg_1	71	73	PF00400	0.555
TRG_NES_CRM1_1	386	399	PF08389	0.568
TRG_NES_CRM1_1	560	572	PF08389	0.572
TRG_NLS_MonoExtN_4	154	160	PF00514	0.411
TRG_Pf-PMV_PEXEL_1	163	168	PF00026	0.570
TRG_Pf-PMV_PEXEL_1	281	286	PF00026	0.492

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1IJU9	Leptomonas seymouri	47%	100%
A0A1X0NY20	Trypanosomatidae	31%	100%
A0A3S7WVR9	Leishmania donovani	93%	100%
A0A422NEI4	Trypanosoma rangeli	30%	100%
A4HYM1	Leishmania infantum	93%	100%
C9ZHT5	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	30%	100%
E9AI85	Leishmania braziliensis	73%	100%
E9AUH0	Leishmania mexicana (strain MHOM/GT/2001/U1103)	89%	100%