Checkpoint protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Checkpoint protein

Gene product:

Cell cycle checkpoint protein RAD1-like, putative

Species:

Leishmania major

UniProt:

Q4QD01_LEIMA

TriTrypDb:

LmjF.20.0390 , LMJLV39_200009600 , LMJSD75_200009400

Length:

362

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	12
GO:0030896	checkpoint clamp complex	3	2
GO:0032991	protein-containing complex	1	2
GO:0043226	organelle	2	12
GO:0043227	membrane-bounded organelle	3	12
GO:0043229	intracellular organelle	3	12
GO:0043231	intracellular membrane-bounded organelle	4	12
GO:0110165	cellular anatomical entity	1	12
GO:0140513	nuclear protein-containing complex	2	2

Expansion

Sequence features

Q4QD01

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4QD01

Function

Biological processes
Term	Name	Level	Count
GO:0000075	cell cycle checkpoint signaling	4	12
GO:0000077	DNA damage checkpoint signaling	5	12
GO:0006139	nucleobase-containing compound metabolic process	3	12
GO:0006259	DNA metabolic process	4	12
GO:0006281	DNA repair	5	12
GO:0006725	cellular aromatic compound metabolic process	3	12
GO:0006807	nitrogen compound metabolic process	2	12
GO:0006950	response to stress	2	12
GO:0006974	DNA damage response	4	12
GO:0007165	signal transduction	2	12
GO:0008152	metabolic process	1	12
GO:0009987	cellular process	1	12
GO:0010564	regulation of cell cycle process	5	12
GO:0010948	negative regulation of cell cycle process	6	12
GO:0031570	DNA integrity checkpoint signaling	5	12
GO:0033554	cellular response to stress	3	12
GO:0034641	cellular nitrogen compound metabolic process	3	12
GO:0035556	intracellular signal transduction	3	12
GO:0042770	signal transduction in response to DNA damage	4	12
GO:0043170	macromolecule metabolic process	3	12
GO:0044237	cellular metabolic process	2	12
GO:0044238	primary metabolic process	2	12
GO:0044260	obsolete cellular macromolecule metabolic process	3	12
GO:0045786	negative regulation of cell cycle	5	12
GO:0046483	heterocycle metabolic process	3	12
GO:0048519	negative regulation of biological process	3	12
GO:0048523	negative regulation of cellular process	4	12
GO:0050789	regulation of biological process	2	12
GO:0050794	regulation of cellular process	3	12
GO:0050896	response to stimulus	1	12
GO:0051716	cellular response to stimulus	2	12
GO:0051726	regulation of cell cycle	4	12
GO:0065007	biological regulation	1	12
GO:0071704	organic substance metabolic process	2	12
GO:0090304	nucleic acid metabolic process	4	12
GO:1901360	organic cyclic compound metabolic process	3	12
GO:1901987	regulation of cell cycle phase transition	6	12
GO:1901988	negative regulation of cell cycle phase transition	7	12

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	1
GO:0004518	nuclease activity	4	1
GO:0004527	exonuclease activity	5	1
GO:0004529	DNA exonuclease activity	5	1
GO:0004536	DNA nuclease activity	4	1
GO:0008853	obsolete exodeoxyribonuclease III activity	7	1
GO:0016787	hydrolase activity	2	1
GO:0016788	hydrolase activity, acting on ester bonds	3	1
GO:0016796	exonuclease activity, active with either ribo- or deoxyribonucleic acids and producing 5'-phosphomonoesters	6	1
GO:0016895	DNA exonuclease activity, producing 5'-phosphomonoesters	6	1
GO:0140097	catalytic activity, acting on DNA	3	1
GO:0140640	catalytic activity, acting on a nucleic acid	2	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	279	283	PF00656	0.727
CLV_C14_Caspase3-7	75	79	PF00656	0.410
CLV_MEL_PAP_1	157	163	PF00089	0.493
CLV_PCSK_SKI1_1	56	60	PF00082	0.437
CLV_PCSK_SKI1_1	68	72	PF00082	0.350
CLV_PCSK_SKI1_1	93	97	PF00082	0.502
DEG_APCC_DBOX_1	152	160	PF00400	0.594
DOC_CYCLIN_RxL_1	53	61	PF00134	0.486
DOC_MAPK_gen_1	150	159	PF00069	0.467
DOC_MAPK_HePTP_8	192	204	PF00069	0.554
DOC_MAPK_MEF2A_6	153	161	PF00069	0.482
DOC_MAPK_MEF2A_6	195	204	PF00069	0.560
DOC_MAPK_MEF2A_6	340	348	PF00069	0.506
DOC_MAPK_MEF2A_6	6	14	PF00069	0.414
DOC_PP2B_LxvP_1	71	74	PF13499	0.470
DOC_USP7_MATH_1	186	190	PF00917	0.523
DOC_USP7_MATH_1	302	306	PF00917	0.733
DOC_USP7_MATH_1	354	358	PF00917	0.574
DOC_USP7_MATH_1	88	92	PF00917	0.612
DOC_WW_Pin1_4	107	112	PF00397	0.545
DOC_WW_Pin1_4	6	11	PF00397	0.506
LIG_14-3-3_CanoR_1	141	147	PF00244	0.558
LIG_14-3-3_CanoR_1	15	21	PF00244	0.473
LIG_14-3-3_CanoR_1	160	166	PF00244	0.337
LIG_14-3-3_CanoR_1	237	245	PF00244	0.488
LIG_14-3-3_CanoR_1	322	326	PF00244	0.496
LIG_14-3-3_CanoR_1	331	336	PF00244	0.436
LIG_Actin_WH2_2	145	162	PF00022	0.556
LIG_APCC_ABBA_1	37	42	PF00400	0.423
LIG_BIR_II_1	1	5	PF00653	0.388
LIG_deltaCOP1_diTrp_1	25	32	PF00928	0.385
LIG_FHA_1	110	116	PF00498	0.544
LIG_FHA_1	147	153	PF00498	0.441
LIG_FHA_1	17	23	PF00498	0.456
LIG_FHA_1	238	244	PF00498	0.437
LIG_FHA_1	322	328	PF00498	0.457
LIG_FHA_1	347	353	PF00498	0.409
LIG_FHA_1	49	55	PF00498	0.462
LIG_FHA_1	90	96	PF00498	0.484
LIG_FHA_2	108	114	PF00498	0.498
LIG_FHA_2	129	135	PF00498	0.715
LIG_FHA_2	174	180	PF00498	0.486
LIG_FHA_2	336	342	PF00498	0.516
LIG_FHA_2	73	79	PF00498	0.426
LIG_LIR_Gen_1	2	12	PF02991	0.410
LIG_LIR_Gen_1	25	32	PF02991	0.400
LIG_LIR_Gen_1	289	297	PF02991	0.668
LIG_LIR_Gen_1	61	70	PF02991	0.516
LIG_LIR_Nem_3	2	7	PF02991	0.399
LIG_LIR_Nem_3	25	30	PF02991	0.403
LIG_LIR_Nem_3	315	320	PF02991	0.495
LIG_LIR_Nem_3	61	65	PF02991	0.475
LIG_NRBOX	142	148	PF00104	0.550
LIG_PDZ_Class_3	357	362	PF00595	0.618
LIG_PTB_Apo_2	284	291	PF02174	0.684
LIG_PTB_Phospho_1	284	290	PF10480	0.687
LIG_SH2_CRK	4	8	PF00017	0.412
LIG_SH2_NCK_1	62	66	PF00017	0.527
LIG_SH2_STAP1	239	243	PF00017	0.533
LIG_SH2_STAT5	239	242	PF00017	0.540
LIG_SH2_STAT5	290	293	PF00017	0.673
LIG_SH2_STAT5	4	7	PF00017	0.389
LIG_SH3_3	348	354	PF00018	0.434
LIG_SUMO_SIM_anti_2	349	354	PF11976	0.473
LIG_SUMO_SIM_par_1	105	110	PF11976	0.398
LIG_SUMO_SIM_par_1	18	23	PF11976	0.429
LIG_SUMO_SIM_par_1	221	229	PF11976	0.573
MOD_CDK_SPxxK_3	107	114	PF00069	0.600
MOD_CK1_1	233	239	PF00069	0.524
MOD_CK1_1	258	264	PF00069	0.768
MOD_CK1_1	86	92	PF00069	0.557
MOD_GlcNHglycan	124	127	PF01048	0.736
MOD_GlcNHglycan	166	169	PF01048	0.420
MOD_GlcNHglycan	231	235	PF01048	0.532
MOD_GlcNHglycan	258	261	PF01048	0.691
MOD_GlcNHglycan	297	300	PF01048	0.660
MOD_GlcNHglycan	304	307	PF01048	0.609
MOD_GSK3_1	142	149	PF00069	0.472
MOD_GSK3_1	16	23	PF00069	0.432
MOD_GSK3_1	164	171	PF00069	0.318
MOD_GSK3_1	182	189	PF00069	0.479
MOD_GSK3_1	226	233	PF00069	0.512
MOD_GSK3_1	255	262	PF00069	0.761
MOD_GSK3_1	26	33	PF00069	0.332
MOD_GSK3_1	331	338	PF00069	0.433
MOD_GSK3_1	46	53	PF00069	0.420
MOD_N-GLC_1	46	51	PF02516	0.537
MOD_NEK2_1	142	147	PF00069	0.466
MOD_NEK2_1	273	278	PF00069	0.762
MOD_NEK2_1	295	300	PF00069	0.620
MOD_NEK2_1	32	37	PF00069	0.455
MOD_NEK2_1	48	53	PF00069	0.455
MOD_NEK2_2	312	317	PF00069	0.643
MOD_PIKK_1	128	134	PF00454	0.762
MOD_PIKK_1	335	341	PF00454	0.540
MOD_PK_1	331	337	PF00069	0.433
MOD_PKA_2	159	165	PF00069	0.552
MOD_PKA_2	273	279	PF00069	0.786
MOD_PKA_2	283	289	PF00069	0.594
MOD_PKA_2	321	327	PF00069	0.464
MOD_Plk_1	190	196	PF00069	0.481
MOD_Plk_4	142	148	PF00069	0.507
MOD_Plk_4	26	32	PF00069	0.428
MOD_Plk_4	312	318	PF00069	0.613
MOD_Plk_4	331	337	PF00069	0.423
MOD_Plk_4	346	352	PF00069	0.360
MOD_Plk_4	72	78	PF00069	0.476
MOD_ProDKin_1	107	113	PF00069	0.559
MOD_ProDKin_1	6	12	PF00069	0.504
MOD_SUMO_rev_2	101	106	PF00179	0.573
MOD_SUMO_rev_2	191	200	PF00179	0.578
MOD_SUMO_rev_2	341	347	PF00179	0.600
TRG_DiLeu_BaEn_2	342	348	PF01217	0.511
TRG_ENDOCYTIC_2	211	214	PF00928	0.533
TRG_ENDOCYTIC_2	290	293	PF00928	0.673
TRG_ENDOCYTIC_2	4	7	PF00928	0.389
TRG_ENDOCYTIC_2	62	65	PF00928	0.518
TRG_Pf-PMV_PEXEL_1	104	108	PF00026	0.400
TRG_Pf-PMV_PEXEL_1	178	183	PF00026	0.553

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1HUB9	Leptomonas seymouri	68%	100%
A0A0S4J1V0	Bodo saltans	33%	100%
A0A1X0NWX4	Trypanosomatidae	45%	100%
A0A3S7WVU5	Leishmania donovani	98%	100%
A0A422NCM6	Trypanosoma rangeli	42%	100%
A4HYN1	Leishmania infantum	97%	100%
C9ZI59	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	36%	100%
E9AI98	Leishmania braziliensis	87%	100%
E9AUI3	Leishmania mexicana (strain MHOM/GT/2001/U1103)	93%	99%
V5BVI1	Trypanosoma cruzi	43%	100%