Putative tubulin-tyrosine ligase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

tubulin-tyrosine ligase, putative

Species:

Leishmania major

UniProt:

Q4QCW7_LEIMA

TriTrypDb:

LmjF.20.0790 , LMJLV39_200013300 , LMJSD75_200013100

Length:

725

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 18

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 30
NetGPI	no	yes: 0, no: 30

Cellular components
Term	Name	Level	Count
GO:0005929	cilium	4	9
GO:0042995	cell projection	2	9
GO:0043226	organelle	2	9
GO:0043227	membrane-bounded organelle	3	9
GO:0110165	cellular anatomical entity	1	9
GO:0120025	plasma membrane bounded cell projection	3	9
GO:0005874	microtubule	6	5
GO:0099080	supramolecular complex	2	5
GO:0099081	supramolecular polymer	3	5
GO:0099512	supramolecular fiber	4	5
GO:0099513	polymeric cytoskeletal fiber	5	5
GO:0005737	cytoplasm	2	1

Expansion

Sequence features

Q4QCW7

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4QCW7

Function

Biological processes
Term	Name	Level	Count
GO:0000226	microtubule cytoskeleton organization	3	5
GO:0006807	nitrogen compound metabolic process	2	31
GO:0006996	organelle organization	4	5
GO:0007010	cytoskeleton organization	5	5
GO:0007017	microtubule-based process	2	5
GO:0008152	metabolic process	1	31
GO:0009987	cellular process	1	5
GO:0016043	cellular component organization	3	5
GO:0018095	protein polyglutamylation	7	9
GO:0018193	peptidyl-amino acid modification	5	9
GO:0018200	peptidyl-glutamic acid modification	6	9
GO:0019538	protein metabolic process	3	31
GO:0036211	protein modification process	4	31
GO:0043170	macromolecule metabolic process	3	31
GO:0043412	macromolecule modification	4	31
GO:0044238	primary metabolic process	2	31
GO:0071704	organic substance metabolic process	2	31
GO:0071840	cellular component organization or biogenesis	2	5
GO:1901564	organonitrogen compound metabolic process	3	31

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	29
GO:0004835	tubulin-tyrosine ligase activity	3	12
GO:0005488	binding	1	14
GO:0005515	protein binding	2	5
GO:0008092	cytoskeletal protein binding	3	5
GO:0015631	tubulin binding	4	5
GO:0016874	ligase activity	2	29
GO:0016879	ligase activity, forming carbon-nitrogen bonds	3	13
GO:0016881	acid-amino acid ligase activity	4	13
GO:0070739	protein-glutamic acid ligase activity	3	5
GO:0070740	tubulin-glutamic acid ligase activity	4	5
GO:0140096	catalytic activity, acting on a protein	2	13
GO:0000166	nucleotide binding	3	11
GO:0005524	ATP binding	5	11
GO:0017076	purine nucleotide binding	4	11
GO:0030554	adenyl nucleotide binding	5	11
GO:0032553	ribonucleotide binding	3	11
GO:0032555	purine ribonucleotide binding	4	11
GO:0032559	adenyl ribonucleotide binding	5	11
GO:0035639	purine ribonucleoside triphosphate binding	4	11
GO:0036094	small molecule binding	2	11
GO:0043167	ion binding	2	11
GO:0043168	anion binding	3	11
GO:0043169	cation binding	3	6
GO:0046872	metal ion binding	4	6
GO:0097159	organic cyclic compound binding	2	11
GO:0097367	carbohydrate derivative binding	2	11
GO:1901265	nucleoside phosphate binding	3	11
GO:1901363	heterocyclic compound binding	2	11

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	700	704	PF00656	0.494
CLV_C14_Caspase3-7	711	715	PF00656	0.318
CLV_NRD_NRD_1	109	111	PF00675	0.650
CLV_NRD_NRD_1	197	199	PF00675	0.355
CLV_NRD_NRD_1	207	209	PF00675	0.370
CLV_NRD_NRD_1	211	213	PF00675	0.381
CLV_NRD_NRD_1	337	339	PF00675	0.333
CLV_NRD_NRD_1	37	39	PF00675	0.431
CLV_NRD_NRD_1	382	384	PF00675	0.317
CLV_NRD_NRD_1	524	526	PF00675	0.355
CLV_NRD_NRD_1	675	677	PF00675	0.362
CLV_PCSK_KEX2_1	109	111	PF00082	0.647
CLV_PCSK_KEX2_1	207	209	PF00082	0.337
CLV_PCSK_KEX2_1	211	213	PF00082	0.332
CLV_PCSK_KEX2_1	35	37	PF00082	0.561
CLV_PCSK_KEX2_1	524	526	PF00082	0.324
CLV_PCSK_KEX2_1	646	648	PF00082	0.510
CLV_PCSK_KEX2_1	674	676	PF00082	0.383
CLV_PCSK_PC1ET2_1	35	37	PF00082	0.436
CLV_PCSK_PC1ET2_1	646	648	PF00082	0.614
CLV_PCSK_PC7_1	207	213	PF00082	0.291
CLV_PCSK_SKI1_1	152	156	PF00082	0.492
CLV_PCSK_SKI1_1	166	170	PF00082	0.427
CLV_PCSK_SKI1_1	339	343	PF00082	0.205
CLV_PCSK_SKI1_1	503	507	PF00082	0.260
CLV_PCSK_SKI1_1	514	518	PF00082	0.255
CLV_PCSK_SKI1_1	588	592	PF00082	0.334
CLV_Separin_Metazoa	500	504	PF03568	0.534
DEG_APCC_DBOX_1	404	412	PF00400	0.534
DEG_APCC_DBOX_1	587	595	PF00400	0.531
DEG_SCF_FBW7_2	355	362	PF00400	0.381
DEG_SPOP_SBC_1	420	424	PF00917	0.412
DEG_SPOP_SBC_1	9	13	PF00917	0.578
DOC_CKS1_1	356	361	PF01111	0.381
DOC_CYCLIN_yCln2_LP_2	625	628	PF00134	0.417
DOC_MAPK_gen_1	198	206	PF00069	0.454
DOC_MAPK_gen_1	35	43	PF00069	0.451
DOC_MAPK_gen_1	383	391	PF00069	0.534
DOC_MAPK_gen_1	692	699	PF00069	0.607
DOC_MAPK_JIP1_4	588	594	PF00069	0.534
DOC_MAPK_MEF2A_6	35	43	PF00069	0.462
DOC_MAPK_MEF2A_6	383	391	PF00069	0.473
DOC_MAPK_MEF2A_6	497	506	PF00069	0.459
DOC_MAPK_MEF2A_6	588	596	PF00069	0.478
DOC_MAPK_NFAT4_5	384	392	PF00069	0.475
DOC_PP1_RVXF_1	501	507	PF00149	0.453
DOC_PP2B_LxvP_1	625	628	PF13499	0.417
DOC_PP4_MxPP_1	626	629	PF00568	0.495
DOC_USP7_MATH_1	113	117	PF00917	0.677
DOC_USP7_MATH_1	238	242	PF00917	0.535
DOC_USP7_MATH_1	263	267	PF00917	0.535
DOC_USP7_MATH_1	431	435	PF00917	0.426
DOC_USP7_MATH_1	461	465	PF00917	0.529
DOC_USP7_MATH_1	466	470	PF00917	0.507
DOC_USP7_MATH_1	532	536	PF00917	0.531
DOC_USP7_MATH_1	540	544	PF00917	0.541
DOC_USP7_MATH_1	574	578	PF00917	0.501
DOC_USP7_MATH_1	580	584	PF00917	0.538
DOC_USP7_MATH_1	708	712	PF00917	0.526
DOC_USP7_MATH_1	97	101	PF00917	0.728
DOC_WW_Pin1_4	129	134	PF00397	0.454
DOC_WW_Pin1_4	240	245	PF00397	0.492
DOC_WW_Pin1_4	29	34	PF00397	0.392
DOC_WW_Pin1_4	328	333	PF00397	0.417
DOC_WW_Pin1_4	355	360	PF00397	0.445
DOC_WW_Pin1_4	411	416	PF00397	0.471
DOC_WW_Pin1_4	507	512	PF00397	0.514
DOC_WW_Pin1_4	536	541	PF00397	0.405
DOC_WW_Pin1_4	549	554	PF00397	0.395
DOC_WW_Pin1_4	570	575	PF00397	0.469
DOC_WW_Pin1_4	704	709	PF00397	0.568
LIG_14-3-3_CanoR_1	114	120	PF00244	0.615
LIG_14-3-3_CanoR_1	122	126	PF00244	0.365
LIG_14-3-3_CanoR_1	338	342	PF00244	0.403
LIG_14-3-3_CanoR_1	410	415	PF00244	0.430
LIG_14-3-3_CanoR_1	518	523	PF00244	0.586
LIG_14-3-3_CanoR_1	657	664	PF00244	0.528
LIG_Actin_WH2_2	502	520	PF00022	0.440
LIG_APCC_ABBA_1	401	406	PF00400	0.467
LIG_APCC_ABBAyCdc20_2	400	406	PF00400	0.458
LIG_BRCT_BRCA1_1	551	555	PF00533	0.534
LIG_EH_1	375	379	PF12763	0.534
LIG_EH1_1	557	565	PF00400	0.439
LIG_FHA_1	133	139	PF00498	0.368
LIG_FHA_1	157	163	PF00498	0.391
LIG_FHA_1	191	197	PF00498	0.457
LIG_FHA_1	415	421	PF00498	0.539
LIG_FHA_1	508	514	PF00498	0.534
LIG_FHA_1	679	685	PF00498	0.449
LIG_FHA_2	12	18	PF00498	0.700
LIG_FHA_2	21	27	PF00498	0.559
LIG_FHA_2	312	318	PF00498	0.405
LIG_FHA_2	338	344	PF00498	0.405
LIG_FHA_2	392	398	PF00498	0.485
LIG_FHA_2	668	674	PF00498	0.384
LIG_LIR_Apic_2	245	250	PF02991	0.484
LIG_LIR_Gen_1	132	141	PF02991	0.468
LIG_LIR_Gen_1	181	191	PF02991	0.443
LIG_LIR_Gen_1	552	563	PF02991	0.534
LIG_LIR_Gen_1	601	611	PF02991	0.439
LIG_LIR_Gen_1	678	685	PF02991	0.427
LIG_LIR_Nem_3	132	137	PF02991	0.406
LIG_LIR_Nem_3	181	187	PF02991	0.445
LIG_LIR_Nem_3	394	399	PF02991	0.492
LIG_LIR_Nem_3	40	46	PF02991	0.399
LIG_LIR_Nem_3	551	557	PF02991	0.460
LIG_LIR_Nem_3	601	607	PF02991	0.439
LIG_LIR_Nem_3	678	683	PF02991	0.403
LIG_LIR_Nem_3	691	696	PF02991	0.440
LIG_MLH1_MIPbox_1	551	555	PF16413	0.417
LIG_NRBOX	201	207	PF00104	0.417
LIG_PCNA_PIPBox_1	597	606	PF02747	0.451
LIG_PCNA_yPIPBox_3	365	374	PF02747	0.399
LIG_Pex14_2	554	558	PF04695	0.503
LIG_SH2_CRK	120	124	PF00017	0.393
LIG_SH2_CRK	184	188	PF00017	0.521
LIG_SH2_CRK	396	400	PF00017	0.534
LIG_SH2_CRK	46	50	PF00017	0.468
LIG_SH2_CRK	693	697	PF00017	0.407
LIG_SH2_NCK_1	46	50	PF00017	0.468
LIG_SH2_NCK_1	650	654	PF00017	0.365
LIG_SH2_SRC	396	399	PF00017	0.496
LIG_SH2_STAP1	416	420	PF00017	0.405
LIG_SH2_STAT3	368	371	PF00017	0.510
LIG_SH2_STAT5	125	128	PF00017	0.421
LIG_SH2_STAT5	28	31	PF00017	0.501
LIG_SH2_STAT5	368	371	PF00017	0.492
LIG_SH2_STAT5	373	376	PF00017	0.478
LIG_SH2_STAT5	390	393	PF00017	0.482
LIG_SH2_STAT5	402	405	PF00017	0.498
LIG_SH2_STAT5	416	419	PF00017	0.476
LIG_SH2_STAT5	483	486	PF00017	0.534
LIG_SH3_1	534	540	PF00018	0.399
LIG_SH3_3	27	33	PF00018	0.535
LIG_SH3_3	3	9	PF00018	0.675
LIG_SH3_3	534	540	PF00018	0.508
LIG_SH3_4	276	283	PF00018	0.405
LIG_SUMO_SIM_anti_2	561	567	PF11976	0.534
LIG_SUMO_SIM_anti_2	618	624	PF11976	0.430
LIG_SUMO_SIM_par_1	561	567	PF11976	0.534
LIG_SUMO_SIM_par_1	588	593	PF11976	0.489
LIG_TRAF2_1	315	318	PF00917	0.405
LIG_TRAF2_1	58	61	PF00917	0.684
LIG_TRAF2_1	669	672	PF00917	0.334
LIG_TYR_ITSM	392	399	PF00017	0.417
MOD_CDC14_SPxK_1	32	35	PF00782	0.456
MOD_CDK_SPxK_1	29	35	PF00069	0.452
MOD_CDK_SPxK_1	706	712	PF00069	0.613
MOD_CDK_SPxxK_3	29	36	PF00069	0.531
MOD_CDK_SPxxK_3	411	418	PF00069	0.412
MOD_CDK_SPxxK_3	507	514	PF00069	0.534
MOD_CK1_1	10	16	PF00069	0.760
MOD_CK1_1	121	127	PF00069	0.552
MOD_CK1_1	328	334	PF00069	0.564
MOD_CK1_1	414	420	PF00069	0.543
MOD_CK1_1	476	482	PF00069	0.453
MOD_CK1_1	535	541	PF00069	0.477
MOD_CK1_1	544	550	PF00069	0.491
MOD_CK1_1	569	575	PF00069	0.409
MOD_CK1_1	578	584	PF00069	0.397
MOD_CK1_1	599	605	PF00069	0.476
MOD_CK1_1	706	712	PF00069	0.559
MOD_CK1_1	92	98	PF00069	0.760
MOD_CK2_1	12	18	PF00069	0.703
MOD_CK2_1	311	317	PF00069	0.417
MOD_CK2_1	337	343	PF00069	0.410
MOD_CK2_1	475	481	PF00069	0.483
MOD_CK2_1	55	61	PF00069	0.678
MOD_CK2_1	656	662	PF00069	0.333
MOD_CK2_1	667	673	PF00069	0.399
MOD_Cter_Amidation	196	199	PF01082	0.258
MOD_Cter_Amidation	209	212	PF01082	0.258
MOD_Cter_Amidation	381	384	PF01082	0.334
MOD_Cter_Amidation	644	647	PF01082	0.699
MOD_GlcNHglycan	115	118	PF01048	0.722
MOD_GlcNHglycan	14	17	PF01048	0.637
MOD_GlcNHglycan	149	152	PF01048	0.524
MOD_GlcNHglycan	180	183	PF01048	0.190
MOD_GlcNHglycan	215	218	PF01048	0.312
MOD_GlcNHglycan	223	226	PF01048	0.313
MOD_GlcNHglycan	265	268	PF01048	0.384
MOD_GlcNHglycan	322	325	PF01048	0.239
MOD_GlcNHglycan	348	351	PF01048	0.335
MOD_GlcNHglycan	423	426	PF01048	0.366
MOD_GlcNHglycan	463	466	PF01048	0.247
MOD_GlcNHglycan	468	471	PF01048	0.224
MOD_GlcNHglycan	490	494	PF01048	0.316
MOD_GlcNHglycan	526	529	PF01048	0.293
MOD_GlcNHglycan	57	60	PF01048	0.778
MOD_GlcNHglycan	576	580	PF01048	0.365
MOD_GlcNHglycan	582	585	PF01048	0.396
MOD_GlcNHglycan	634	637	PF01048	0.669
MOD_GlcNHglycan	647	650	PF01048	0.542
MOD_GlcNHglycan	688	691	PF01048	0.501
MOD_GlcNHglycan	698	702	PF01048	0.606
MOD_GlcNHglycan	91	94	PF01048	0.755
MOD_GSK3_1	213	220	PF00069	0.508
MOD_GSK3_1	221	228	PF00069	0.522
MOD_GSK3_1	337	344	PF00069	0.546
MOD_GSK3_1	410	417	PF00069	0.505
MOD_GSK3_1	421	428	PF00069	0.499
MOD_GSK3_1	461	468	PF00069	0.473
MOD_GSK3_1	532	539	PF00069	0.529
MOD_GSK3_1	540	547	PF00069	0.542
MOD_GSK3_1	55	62	PF00069	0.683
MOD_GSK3_1	566	573	PF00069	0.481
MOD_GSK3_1	574	581	PF00069	0.469
MOD_GSK3_1	598	605	PF00069	0.477
MOD_GSK3_1	627	634	PF00069	0.541
MOD_GSK3_1	7	14	PF00069	0.655
MOD_GSK3_1	702	709	PF00069	0.634
MOD_GSK3_1	716	723	PF00069	0.423
MOD_N-GLC_1	441	446	PF02516	0.273
MOD_N-GLC_1	548	553	PF02516	0.200
MOD_N-GLC_1	678	683	PF02516	0.348
MOD_N-GLC_2	665	667	PF02516	0.322
MOD_NEK2_1	127	132	PF00069	0.384
MOD_NEK2_1	137	142	PF00069	0.377
MOD_NEK2_1	156	161	PF00069	0.280
MOD_NEK2_1	327	332	PF00069	0.415
MOD_NEK2_1	341	346	PF00069	0.575
MOD_NEK2_1	517	522	PF00069	0.540
MOD_NEK2_1	697	702	PF00069	0.503
MOD_NEK2_1	89	94	PF00069	0.575
MOD_NEK2_2	296	301	PF00069	0.381
MOD_PIKK_1	104	110	PF00454	0.537
MOD_PIKK_1	363	369	PF00454	0.397
MOD_PK_1	518	524	PF00069	0.497
MOD_PKA_1	37	43	PF00069	0.463
MOD_PKA_1	524	530	PF00069	0.399
MOD_PKA_2	113	119	PF00069	0.675
MOD_PKA_2	121	127	PF00069	0.374
MOD_PKA_2	147	153	PF00069	0.555
MOD_PKA_2	337	343	PF00069	0.405
MOD_PKA_2	37	43	PF00069	0.464
MOD_PKA_2	517	523	PF00069	0.589
MOD_PKA_2	524	530	PF00069	0.399
MOD_PKA_2	639	645	PF00069	0.681
MOD_PKA_2	656	662	PF00069	0.458
MOD_PKA_2	686	692	PF00069	0.527
MOD_Plk_1	441	447	PF00069	0.473
MOD_Plk_1	678	684	PF00069	0.497
MOD_Plk_2-3	656	662	PF00069	0.333
MOD_Plk_4	1	7	PF00069	0.714
MOD_Plk_4	121	127	PF00069	0.445
MOD_Plk_4	158	164	PF00069	0.422
MOD_Plk_4	168	174	PF00069	0.535
MOD_Plk_4	243	249	PF00069	0.523
MOD_Plk_4	602	608	PF00069	0.444
MOD_Plk_4	618	624	PF00069	0.479
MOD_Plk_4	64	70	PF00069	0.715
MOD_Plk_4	716	722	PF00069	0.373
MOD_Plk_4	97	103	PF00069	0.752
MOD_ProDKin_1	129	135	PF00069	0.450
MOD_ProDKin_1	240	246	PF00069	0.492
MOD_ProDKin_1	29	35	PF00069	0.400
MOD_ProDKin_1	328	334	PF00069	0.417
MOD_ProDKin_1	355	361	PF00069	0.439
MOD_ProDKin_1	411	417	PF00069	0.471
MOD_ProDKin_1	507	513	PF00069	0.514
MOD_ProDKin_1	536	542	PF00069	0.405
MOD_ProDKin_1	549	555	PF00069	0.395
MOD_ProDKin_1	570	576	PF00069	0.469
MOD_ProDKin_1	704	710	PF00069	0.554
MOD_SUMO_rev_2	432	438	PF00179	0.601
MOD_SUMO_rev_2	469	476	PF00179	0.419
TRG_DiLeu_BaEn_1	500	505	PF01217	0.461
TRG_DiLeu_BaLyEn_6	679	684	PF01217	0.447
TRG_ENDOCYTIC_2	120	123	PF00928	0.427
TRG_ENDOCYTIC_2	184	187	PF00928	0.503
TRG_ENDOCYTIC_2	396	399	PF00928	0.490
TRG_ENDOCYTIC_2	604	607	PF00928	0.439
TRG_ENDOCYTIC_2	693	696	PF00928	0.338
TRG_ER_diArg_1	109	111	PF00400	0.643
TRG_ER_diArg_1	206	208	PF00400	0.544
TRG_ER_diArg_1	211	213	PF00400	0.536
TRG_ER_diArg_1	36	38	PF00400	0.422
TRG_ER_diArg_1	674	676	PF00400	0.389
TRG_NES_CRM1_1	500	512	PF08389	0.417
TRG_NES_CRM1_1	556	567	PF08389	0.417
TRG_NLS_MonoCore_2	34	39	PF00514	0.348
TRG_NLS_MonoExtC_3	34	39	PF00514	0.357
TRG_NLS_MonoExtN_4	33	39	PF00514	0.359
TRG_Pf-PMV_PEXEL_1	258	262	PF00026	0.217
TRG_Pf-PMV_PEXEL_1	339	343	PF00026	0.205
TRG_Pf-PMV_PEXEL_1	694	698	PF00026	0.492

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P7G7	Leptomonas seymouri	43%	89%
A0A0N1I1Q6	Leptomonas seymouri	27%	100%
A0A0N1IAP5	Leptomonas seymouri	27%	98%
A0A0S4ISF0	Bodo saltans	24%	100%
A0A0S4KQK8	Bodo saltans	28%	100%
A0A1X0NXX8	Trypanosomatidae	32%	100%
A0A3Q8IDL7	Leishmania donovani	92%	100%
A0A3Q8IEC1	Leishmania donovani	27%	100%
A0A3Q8III2	Leishmania donovani	27%	100%
A0A3S5H6X0	Leishmania donovani	23%	89%
A0A422NPL9	Trypanosoma rangeli	33%	100%
A4H8G3	Leishmania braziliensis	24%	99%
A4HIG0	Leishmania braziliensis	27%	100%
A4HNF6	Leishmania braziliensis	27%	100%
A4HWT7	Leishmania infantum	24%	89%
A4HYN9	Leishmania infantum	93%	100%
A4I5Q6	Leishmania infantum	26%	100%
A4IC28	Leishmania infantum	27%	100%
C9ZI96	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	34%	100%
C9ZR25	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	26%	100%
E9AFW7	Leishmania major	27%	100%
E9AID0	Leishmania braziliensis	76%	97%
E9AQJ5	Leishmania mexicana (strain MHOM/GT/2001/U1103)	25%	89%
E9AUL7	Leishmania mexicana (strain MHOM/GT/2001/U1103)	90%	100%
E9B101	Leishmania mexicana (strain MHOM/GT/2001/U1103)	25%	100%
E9B720	Leishmania mexicana (strain MHOM/GT/2001/U1103)	26%	100%
Q4Q765	Leishmania major	25%	100%
Q4QEY2	Leishmania major	24%	90%
V5BVF5	Trypanosoma cruzi	34%	100%