Putative calpain-like cysteine peptidase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

calpain-like cysteine peptidase, putative

Species:

Leishmania major

UniProt:

Q4QCS9_LEIMA

TriTrypDb:

LmjF.20.1180 , LMJLV39_200017700 , LMJSD75_040009600

Length:

976

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 1
Forrest at al. (metacyclic)	no	yes: 3
Forrest at al. (procyclic)	no	yes: 4
Silverman et al.	no	yes: 0
Pissara et al.	yes	yes: 36

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 16

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 20

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 2, no: 93
NetGPI	no	yes: 0, no: 95

Cellular components
Term	Name	Level	Count
GO:0016020	membrane	2	1
GO:0110165	cellular anatomical entity	1	5
GO:0005737	cytoplasm	2	3
GO:0005930	axoneme	2	1
GO:0005815	microtubule organizing center	2	1
GO:0005856	cytoskeleton	5	1
GO:0020016	ciliary pocket	2	1
GO:0020038	subpellicular network	2	1
GO:0030863	cortical cytoskeleton	6	1
GO:0036064	ciliary basal body	3	1
GO:0043226	organelle	2	1
GO:0043228	non-membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043232	intracellular non-membrane-bounded organelle	4	1

Expansion

Sequence features

Q4QCS9

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4QCS9

Function

Biological processes
Term	Name	Level	Count
GO:0006508	proteolysis	4	96
GO:0006807	nitrogen compound metabolic process	2	96
GO:0008152	metabolic process	1	96
GO:0019538	protein metabolic process	3	96
GO:0043170	macromolecule metabolic process	3	96
GO:0044238	primary metabolic process	2	96
GO:0071704	organic substance metabolic process	2	96
GO:1901564	organonitrogen compound metabolic process	3	96

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	96
GO:0004175	endopeptidase activity	4	96
GO:0004197	cysteine-type endopeptidase activity	5	96
GO:0004198	calcium-dependent cysteine-type endopeptidase activity	6	96
GO:0008233	peptidase activity	3	96
GO:0008234	cysteine-type peptidase activity	4	96
GO:0016787	hydrolase activity	2	96
GO:0140096	catalytic activity, acting on a protein	2	96

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	24	28	PF00656	0.789
CLV_C14_Caspase3-7	297	301	PF00656	0.458
CLV_C14_Caspase3-7	459	463	PF00656	0.574
CLV_C14_Caspase3-7	534	538	PF00656	0.487
CLV_C14_Caspase3-7	773	777	PF00656	0.576
CLV_NRD_NRD_1	440	442	PF00675	0.479
CLV_NRD_NRD_1	777	779	PF00675	0.225
CLV_PCSK_KEX2_1	440	442	PF00082	0.444
CLV_PCSK_KEX2_1	830	832	PF00082	0.455
CLV_PCSK_KEX2_1	930	932	PF00082	0.596
CLV_PCSK_KEX2_1	960	962	PF00082	0.329
CLV_PCSK_PC1ET2_1	830	832	PF00082	0.474
CLV_PCSK_PC1ET2_1	930	932	PF00082	0.600
CLV_PCSK_PC1ET2_1	960	962	PF00082	0.328
CLV_PCSK_SKI1_1	283	287	PF00082	0.639
CLV_PCSK_SKI1_1	441	445	PF00082	0.563
CLV_PCSK_SKI1_1	496	500	PF00082	0.365
CLV_PCSK_SKI1_1	587	591	PF00082	0.366
CLV_PCSK_SKI1_1	603	607	PF00082	0.326
CLV_PCSK_SKI1_1	872	876	PF00082	0.495
DEG_SIAH_1	273	281	PF03145	0.429
DOC_CKS1_1	23	28	PF01111	0.545
DOC_PP1_RVXF_1	575	581	PF00149	0.506
DOC_PP1_RVXF_1	733	740	PF00149	0.565
DOC_PP4_FxxP_1	345	348	PF00568	0.335
DOC_PP4_FxxP_1	478	481	PF00568	0.559
DOC_PP4_FxxP_1	558	561	PF00568	0.512
DOC_USP7_MATH_1	492	496	PF00917	0.527
DOC_USP7_MATH_1	545	549	PF00917	0.502
DOC_USP7_MATH_2	511	517	PF00917	0.563
DOC_USP7_UBL2_3	621	625	PF12436	0.512
DOC_USP7_UBL2_3	775	779	PF12436	0.534
DOC_WW_Pin1_4	182	187	PF00397	0.820
DOC_WW_Pin1_4	203	208	PF00397	0.550
DOC_WW_Pin1_4	22	27	PF00397	0.546
DOC_WW_Pin1_4	269	274	PF00397	0.668
DOC_WW_Pin1_4	681	686	PF00397	0.520
DOC_WW_Pin1_4	814	819	PF00397	0.439
LIG_14-3-3_CanoR_1	283	289	PF00244	0.659
LIG_14-3-3_CanoR_1	440	448	PF00244	0.573
LIG_14-3-3_CanoR_1	577	581	PF00244	0.492
LIG_14-3-3_CanoR_1	867	876	PF00244	0.567
LIG_Actin_WH2_2	588	605	PF00022	0.592
LIG_BIR_II_1	1	5	PF00653	0.551
LIG_BIR_III_2	751	755	PF00653	0.420
LIG_BRCT_BRCA1_1	494	498	PF00533	0.541
LIG_BRCT_BRCA1_1	925	929	PF00533	0.580
LIG_Clathr_ClatBox_1	949	953	PF01394	0.343
LIG_CtBP_PxDLS_1	511	515	PF00389	0.422
LIG_deltaCOP1_diTrp_1	738	743	PF00928	0.502
LIG_deltaCOP1_diTrp_1	871	875	PF00928	0.456
LIG_eIF4E_1	700	706	PF01652	0.524
LIG_FHA_1	280	286	PF00498	0.622
LIG_FHA_1	422	428	PF00498	0.370
LIG_FHA_1	442	448	PF00498	0.505
LIG_FHA_1	801	807	PF00498	0.457
LIG_FHA_1	820	826	PF00498	0.458
LIG_FHA_2	11	17	PF00498	0.807
LIG_FHA_2	408	414	PF00498	0.440
LIG_FHA_2	457	463	PF00498	0.614
LIG_FHA_2	710	716	PF00498	0.518
LIG_LIR_Apic_2	343	348	PF02991	0.341
LIG_LIR_Gen_1	620	631	PF02991	0.517
LIG_LIR_Gen_1	646	653	PF02991	0.522
LIG_LIR_Gen_1	664	675	PF02991	0.519
LIG_LIR_Gen_1	698	706	PF02991	0.556
LIG_LIR_Gen_1	819	825	PF02991	0.387
LIG_LIR_Gen_1	870	881	PF02991	0.453
LIG_LIR_Gen_1	9	20	PF02991	0.559
LIG_LIR_Nem_3	361	365	PF02991	0.345
LIG_LIR_Nem_3	495	501	PF02991	0.554
LIG_LIR_Nem_3	592	597	PF02991	0.540
LIG_LIR_Nem_3	613	618	PF02991	0.511
LIG_LIR_Nem_3	620	626	PF02991	0.508
LIG_LIR_Nem_3	629	633	PF02991	0.497
LIG_LIR_Nem_3	664	670	PF02991	0.496
LIG_LIR_Nem_3	698	703	PF02991	0.516
LIG_LIR_Nem_3	819	824	PF02991	0.426
LIG_LIR_Nem_3	838	842	PF02991	0.440
LIG_LIR_Nem_3	852	858	PF02991	0.461
LIG_LIR_Nem_3	870	876	PF02991	0.478
LIG_LIR_Nem_3	9	15	PF02991	0.561
LIG_LIR_Nem_3	926	932	PF02991	0.525
LIG_LIR_Nem_3	940	945	PF02991	0.604
LIG_PDZ_Class_3	971	976	PF00595	0.336
LIG_Pex14_2	770	774	PF04695	0.512
LIG_SH2_CRK	240	244	PF00017	0.583
LIG_SH2_CRK	623	627	PF00017	0.505
LIG_SH2_CRK	630	634	PF00017	0.503
LIG_SH2_CRK	678	682	PF00017	0.515
LIG_SH2_CRK	700	704	PF00017	0.517
LIG_SH2_CRK	839	843	PF00017	0.428
LIG_SH2_CRK	855	859	PF00017	0.469
LIG_SH2_GRB2like	436	439	PF00017	0.596
LIG_SH2_GRB2like	780	783	PF00017	0.475
LIG_SH2_NCK_1	240	244	PF00017	0.569
LIG_SH2_NCK_1	247	251	PF00017	0.817
LIG_SH2_NCK_1	700	704	PF00017	0.512
LIG_SH2_PTP2	892	895	PF00017	0.426
LIG_SH2_SRC	594	597	PF00017	0.521
LIG_SH2_STAP1	224	228	PF00017	0.571
LIG_SH2_STAP1	247	251	PF00017	0.561
LIG_SH2_STAP1	371	375	PF00017	0.359
LIG_SH2_STAP1	604	608	PF00017	0.554
LIG_SH2_STAP1	794	798	PF00017	0.380
LIG_SH2_STAT3	199	202	PF00017	0.860
LIG_SH2_STAT5	199	202	PF00017	0.860
LIG_SH2_STAT5	210	213	PF00017	0.582
LIG_SH2_STAT5	401	404	PF00017	0.372
LIG_SH2_STAT5	594	597	PF00017	0.516
LIG_SH2_STAT5	678	681	PF00017	0.527
LIG_SH2_STAT5	711	714	PF00017	0.494
LIG_SH2_STAT5	780	783	PF00017	0.509
LIG_SH2_STAT5	892	895	PF00017	0.375
LIG_SH2_STAT5	910	913	PF00017	0.384
LIG_SH3_3	152	158	PF00018	0.800
LIG_SH3_3	201	207	PF00018	0.820
LIG_SH3_3	23	29	PF00018	0.545
LIG_SH3_3	246	252	PF00018	0.734
LIG_SH3_3	255	261	PF00018	0.747
LIG_SH3_3	403	409	PF00018	0.363
LIG_SH3_3	478	484	PF00018	0.574
LIG_SH3_3	494	500	PF00018	0.608
LIG_SH3_3	812	818	PF00018	0.416
LIG_SH3_3	901	907	PF00018	0.629
LIG_SH3_3	949	955	PF00018	0.367
LIG_SH3_5	792	796	PF00018	0.477
LIG_SUMO_SIM_anti_2	880	885	PF11976	0.401
LIG_SUMO_SIM_par_1	808	814	PF11976	0.424
LIG_SUMO_SIM_par_1	840	845	PF11976	0.397
LIG_TRAF2_1	118	121	PF00917	0.339
LIG_TRAF2_1	19	22	PF00917	0.781
LIG_TRAF2_1	223	226	PF00917	0.831
LIG_TRAF2_1	40	43	PF00917	0.208
LIG_TRAF2_1	53	56	PF00917	0.200
LIG_TRAF2_1	66	69	PF00917	0.197
LIG_TRAF2_1	79	82	PF00917	0.203
LIG_TRAF2_2	143	148	PF00917	0.546
LIG_TRAF2_2	29	34	PF00917	0.542
LIG_TYR_ITIM	350	355	PF00017	0.365
LIG_TYR_ITIM	837	842	PF00017	0.499
LIG_UBA3_1	350	358	PF00899	0.381
LIG_UBA3_1	769	775	PF00899	0.420
LIG_UBA3_1	883	891	PF00899	0.478
LIG_WRPW_2	498	501	PF00400	0.420
MOD_CDK_SPxK_1	681	687	PF00069	0.518
MOD_CK1_1	159	165	PF00069	0.732
MOD_CK1_1	543	549	PF00069	0.574
MOD_CK2_1	10	16	PF00069	0.749
MOD_CK2_1	299	305	PF00069	0.507
MOD_CK2_1	407	413	PF00069	0.471
MOD_CK2_1	543	549	PF00069	0.518
MOD_CK2_1	709	715	PF00069	0.565
MOD_CK2_1	842	848	PF00069	0.435
MOD_CMANNOS	771	774	PF00535	0.305
MOD_GlcNHglycan	274	277	PF01048	0.636
MOD_GlcNHglycan	473	476	PF01048	0.616
MOD_GlcNHglycan	597	600	PF01048	0.341
MOD_GlcNHglycan	606	609	PF01048	0.385
MOD_GlcNHglycan	634	637	PF01048	0.308
MOD_GlcNHglycan	738	742	PF01048	0.372
MOD_GlcNHglycan	807	810	PF01048	0.545
MOD_GlcNHglycan	861	864	PF01048	0.455
MOD_GSK3_1	643	650	PF00069	0.504
MOD_GSK3_1	676	683	PF00069	0.573
MOD_GSK3_1	819	826	PF00069	0.460
MOD_N-GLC_1	365	370	PF02516	0.359
MOD_N-GLC_1	389	394	PF02516	0.379
MOD_N-GLC_1	859	864	PF02516	0.444
MOD_NEK2_1	306	311	PF00069	0.463
MOD_NEK2_1	388	393	PF00069	0.314
MOD_NEK2_1	416	421	PF00069	0.469
MOD_NEK2_1	535	540	PF00069	0.539
MOD_NEK2_1	590	595	PF00069	0.549
MOD_NEK2_1	626	631	PF00069	0.521
MOD_NEK2_1	653	658	PF00069	0.576
MOD_NEK2_1	770	775	PF00069	0.508
MOD_NEK2_1	859	864	PF00069	0.493
MOD_NEK2_2	647	652	PF00069	0.594
MOD_NEK2_2	923	928	PF00069	0.534
MOD_OFUCOSY	337	344	PF10250	0.229
MOD_PIKK_1	159	165	PF00454	0.768
MOD_PIKK_1	177	183	PF00454	0.643
MOD_PIKK_1	513	519	PF00454	0.578
MOD_PIKK_1	823	829	PF00454	0.391
MOD_PIKK_1	967	973	PF00454	0.354
MOD_PKA_2	10	16	PF00069	0.549
MOD_PKA_2	576	582	PF00069	0.491
MOD_PKA_2	866	872	PF00069	0.561
MOD_Plk_1	325	331	PF00069	0.621
MOD_Plk_1	365	371	PF00069	0.377
MOD_Plk_1	388	394	PF00069	0.345
MOD_Plk_1	653	659	PF00069	0.550
MOD_Plk_1	879	885	PF00069	0.404
MOD_Plk_1	923	929	PF00069	0.545
MOD_Plk_2-3	655	661	PF00069	0.604
MOD_Plk_4	325	331	PF00069	0.613
MOD_Plk_4	535	541	PF00069	0.519
MOD_Plk_4	590	596	PF00069	0.544
MOD_Plk_4	676	682	PF00069	0.619
MOD_Plk_4	709	715	PF00069	0.526
MOD_Plk_4	879	885	PF00069	0.459
MOD_ProDKin_1	182	188	PF00069	0.819
MOD_ProDKin_1	203	209	PF00069	0.552
MOD_ProDKin_1	22	28	PF00069	0.546
MOD_ProDKin_1	269	275	PF00069	0.659
MOD_ProDKin_1	681	687	PF00069	0.520
MOD_ProDKin_1	814	820	PF00069	0.443
MOD_SUMO_for_1	943	946	PF00179	0.334
MOD_SUMO_rev_2	654	663	PF00179	0.571
MOD_SUMO_rev_2	715	724	PF00179	0.553
TRG_DiLeu_BaEn_2	410	416	PF01217	0.298
TRG_ENDOCYTIC_2	247	250	PF00928	0.826
TRG_ENDOCYTIC_2	352	355	PF00928	0.351
TRG_ENDOCYTIC_2	594	597	PF00928	0.534
TRG_ENDOCYTIC_2	623	626	PF00928	0.511
TRG_ENDOCYTIC_2	630	633	PF00928	0.508
TRG_ENDOCYTIC_2	678	681	PF00928	0.506
TRG_ENDOCYTIC_2	700	703	PF00928	0.520
TRG_ENDOCYTIC_2	711	714	PF00928	0.543
TRG_ENDOCYTIC_2	839	842	PF00928	0.403
TRG_ENDOCYTIC_2	855	858	PF00928	0.423
TRG_ENDOCYTIC_2	892	895	PF00928	0.363
TRG_ER_diArg_1	280	283	PF00400	0.637
TRG_Pf-PMV_PEXEL_1	441	445	PF00026	0.614

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P8T6	Leptomonas seymouri	34%	100%
A0A0N1I8N2	Leptomonas seymouri	29%	99%
A0A0N1IGQ2	Leptomonas seymouri	32%	100%
A0A0N1ILF1	Leptomonas seymouri	40%	100%
A0A0N1IMH1	Leptomonas seymouri	34%	100%
A0A0N1P9P1	Leptomonas seymouri	39%	100%
A0A0N1PCA9	Leptomonas seymouri	35%	100%
A0A0N1PE91	Leptomonas seymouri	27%	100%
A0A0S4JLK6	Bodo saltans	27%	100%
A0A1X0NJ19	Trypanosomatidae	28%	80%
A0A1X0NJK2	Trypanosomatidae	31%	100%
A0A1X0NJX8	Trypanosomatidae	36%	100%
A0A1X0NKT7	Trypanosomatidae	32%	100%
A0A1X0NKX8	Trypanosomatidae	32%	100%
A0A1X0NMT3	Trypanosomatidae	42%	100%
A0A1X0NW84	Trypanosomatidae	38%	100%
A0A1X0NW89	Trypanosomatidae	54%	100%
A0A1X0NWA6	Trypanosomatidae	30%	100%
A0A1X0NWW1	Trypanosomatidae	40%	100%
A0A3Q8IBS3	Leishmania donovani	92%	100%
A0A3Q8IDD4	Leishmania donovani	33%	100%
A0A3Q8IJT4	Leishmania donovani	26%	100%
A0A3S5H5A5	Leishmania donovani	41%	100%
A0A3S5ISG2	Trypanosoma rangeli	31%	100%
A0A3S7WW13	Leishmania donovani	27%	88%
A0A3S7WW18	Leishmania donovani	95%	100%
A0A3S7WW41	Leishmania donovani	35%	100%
A0A3S7WW71	Leishmania donovani	39%	100%
A0A3S7X430	Leishmania donovani	34%	100%
A0A3S7X438	Leishmania donovani	30%	100%
A0A3S7X460	Leishmania donovani	35%	100%
A0A3S7X463	Leishmania donovani	27%	100%
A0A3S7X470	Leishmania donovani	35%	100%
A0A422MYU1	Trypanosoma rangeli	52%	100%
A0A422MYX0	Trypanosoma rangeli	39%	100%
A4H3W4	Leishmania braziliensis	41%	100%
A4HE81	Leishmania braziliensis	33%	100%
A4HJ14	Leishmania braziliensis	34%	100%
A4HJ22	Leishmania braziliensis	32%	100%
A4HJ24	Leishmania braziliensis	34%	100%
A4HS39	Leishmania infantum	41%	100%
A4HYN0	Leishmania infantum	95%	100%
A4HYW1	Leishmania infantum	90%	96%
A4HYW2	Leishmania infantum	39%	100%
A4HYW3	Leishmania infantum	35%	100%
A4HYW4	Leishmania infantum	28%	98%
A4I1J4	Leishmania infantum	33%	100%
A4I6E4	Leishmania infantum	35%	100%
A4I6E6	Leishmania infantum	35%	100%
A4I6F0	Leishmania infantum	30%	100%
A4I6K4	Leishmania infantum	34%	100%
A4I6K5	Leishmania infantum	27%	100%
A4I6K6	Leishmania infantum	26%	100%
C9ZIE7	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	44%	91%
C9ZIE8	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	33%	100%
C9ZIE9	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	38%	100%
C9ZN52	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	34%	100%
C9ZY36	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	40%	100%
E8NHG6	Leishmania mexicana (strain MHOM/GT/2001/U1103)	35%	100%
E8NHG7	Leishmania mexicana (strain MHOM/GT/2001/U1103)	31%	100%
E8NHG8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	34%	100%
E8NHM2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	34%	100%
E8NHM4	Leishmania mexicana (strain MHOM/GT/2001/U1103)	34%	100%
E9AIH1	Leishmania braziliensis	84%	100%
E9AIH3	Leishmania braziliensis	39%	100%
E9AIH4	Leishmania braziliensis	35%	100%
E9AK26	Leishmania mexicana (strain MHOM/GT/2001/U1103)	40%	100%
E9AUQ7	Leishmania mexicana (strain MHOM/GT/2001/U1103)	85%	100%
E9AUQ9	Leishmania mexicana (strain MHOM/GT/2001/U1103)	38%	100%
E9AUR0	Leishmania mexicana (strain MHOM/GT/2001/U1103)	35%	100%
E9AXM9	Leishmania mexicana (strain MHOM/GT/2001/U1103)	34%	100%
E9B1J0	Leishmania mexicana (strain MHOM/GT/2001/U1103)	33%	100%
E9B1J1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	26%	100%
E9B1J2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	27%	100%
E9B1J6	Leishmania mexicana (strain MHOM/GT/2001/U1103)	35%	100%
Q4Q6L7	Leishmania major	35%	100%
Q4Q6L9	Leishmania major	35%	100%
Q4Q6M0	Leishmania major	31%	100%
Q4Q6M2	Leishmania major	25%	100%
Q4Q6M3	Leishmania major	27%	100%
Q4Q6M4	Leishmania major	33%	100%
Q4Q9U3	Leishmania major	34%	100%
Q4QCS6	Leishmania major	35%	100%
Q4QCS7	Leishmania major	39%	100%
Q4QCS8	Leishmania major	100%	100%
Q9U0T9	Leishmania major	40%	100%
V5AYJ1	Trypanosoma cruzi	31%	100%
V5B5I4	Trypanosoma cruzi	50%	100%
V5BA05	Trypanosoma cruzi	41%	100%
V5BEL3	Trypanosoma cruzi	36%	100%
V5BII7	Trypanosoma cruzi	28%	79%
V5BN20	Trypanosoma cruzi	35%	100%
V5D9Y2	Trypanosoma cruzi	40%	100%
V5DES7	Trypanosoma cruzi	31%	100%