Putative calpain-like cysteine peptidase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

calpain-like cysteine peptidase, putative

Species:

Leishmania major

UniProt:

Q4QCS8_LEIMA

TriTrypDb:

LmjF.20.1185 , LMJLV39_200017800 , LMJSD75_040009600

Length:

823

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 1
Forrest at al. (metacyclic)	no	yes: 3
Forrest at al. (procyclic)	no	yes: 4
Silverman et al.	no	yes: 0
Pissara et al.	yes	yes: 36

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	yes	yes: 16

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 20

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 2, no: 103
NetGPI	no	yes: 0, no: 105

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	3
GO:0110165	cellular anatomical entity	1	5
GO:0005815	microtubule organizing center	2	1
GO:0005856	cytoskeleton	5	1
GO:0020016	ciliary pocket	2	1
GO:0020038	subpellicular network	2	1
GO:0030863	cortical cytoskeleton	6	1
GO:0036064	ciliary basal body	3	1
GO:0043226	organelle	2	1
GO:0043228	non-membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043232	intracellular non-membrane-bounded organelle	4	1
GO:0005930	axoneme	2	1
GO:0016020	membrane	2	1

Expansion

Sequence features

Q4QCS8

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4QCS8

Function

Biological processes
Term	Name	Level	Count
GO:0006508	proteolysis	4	106
GO:0006807	nitrogen compound metabolic process	2	106
GO:0008152	metabolic process	1	106
GO:0019538	protein metabolic process	3	106
GO:0043170	macromolecule metabolic process	3	106
GO:0044238	primary metabolic process	2	106
GO:0071704	organic substance metabolic process	2	106
GO:1901564	organonitrogen compound metabolic process	3	106

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	106
GO:0004175	endopeptidase activity	4	106
GO:0004197	cysteine-type endopeptidase activity	5	106
GO:0004198	calcium-dependent cysteine-type endopeptidase activity	6	106
GO:0008233	peptidase activity	3	106
GO:0008234	cysteine-type peptidase activity	4	106
GO:0016787	hydrolase activity	2	106
GO:0140096	catalytic activity, acting on a protein	2	106

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	143	147	PF00656	0.483
CLV_C14_Caspase3-7	305	309	PF00656	0.560
CLV_C14_Caspase3-7	380	384	PF00656	0.521
CLV_C14_Caspase3-7	619	623	PF00656	0.598
CLV_NRD_NRD_1	286	288	PF00675	0.517
CLV_NRD_NRD_1	623	625	PF00675	0.464
CLV_PCSK_KEX2_1	286	288	PF00082	0.482
CLV_PCSK_KEX2_1	676	678	PF00082	0.454
CLV_PCSK_KEX2_1	776	778	PF00082	0.549
CLV_PCSK_KEX2_1	806	808	PF00082	0.546
CLV_PCSK_PC1ET2_1	676	678	PF00082	0.469
CLV_PCSK_PC1ET2_1	776	778	PF00082	0.579
CLV_PCSK_PC1ET2_1	806	808	PF00082	0.593
CLV_PCSK_SKI1_1	129	133	PF00082	0.700
CLV_PCSK_SKI1_1	287	291	PF00082	0.614
CLV_PCSK_SKI1_1	342	346	PF00082	0.330
CLV_PCSK_SKI1_1	433	437	PF00082	0.393
CLV_PCSK_SKI1_1	449	453	PF00082	0.357
CLV_PCSK_SKI1_1	718	722	PF00082	0.525
DEG_SIAH_1	119	127	PF03145	0.441
DOC_PP1_RVXF_1	421	427	PF00149	0.549
DOC_PP1_RVXF_1	579	586	PF00149	0.608
DOC_PP4_FxxP_1	191	194	PF00568	0.368
DOC_PP4_FxxP_1	324	327	PF00568	0.592
DOC_PP4_FxxP_1	404	407	PF00568	0.553
DOC_USP7_MATH_1	338	342	PF00917	0.535
DOC_USP7_MATH_1	391	395	PF00917	0.550
DOC_USP7_MATH_2	357	363	PF00917	0.604
DOC_USP7_UBL2_3	467	471	PF12436	0.546
DOC_USP7_UBL2_3	621	625	PF12436	0.576
DOC_WW_Pin1_4	115	120	PF00397	0.682
DOC_WW_Pin1_4	49	54	PF00397	0.851
DOC_WW_Pin1_4	527	532	PF00397	0.554
DOC_WW_Pin1_4	660	665	PF00397	0.470
LIG_14-3-3_CanoR_1	129	135	PF00244	0.676
LIG_14-3-3_CanoR_1	286	294	PF00244	0.588
LIG_14-3-3_CanoR_1	423	427	PF00244	0.534
LIG_14-3-3_CanoR_1	713	722	PF00244	0.597
LIG_Actin_WH2_2	434	451	PF00022	0.631
LIG_BIR_II_1	1	5	PF00653	0.892
LIG_BIR_III_2	597	601	PF00653	0.450
LIG_BRCT_BRCA1_1	340	344	PF00533	0.583
LIG_BRCT_BRCA1_1	771	775	PF00533	0.542
LIG_Clathr_ClatBox_1	795	799	PF01394	0.632
LIG_CtBP_PxDLS_1	357	361	PF00389	0.453
LIG_deltaCOP1_diTrp_1	584	589	PF00928	0.544
LIG_deltaCOP1_diTrp_1	717	721	PF00928	0.503
LIG_eIF4E_1	546	552	PF01652	0.567
LIG_FHA_1	126	132	PF00498	0.653
LIG_FHA_1	268	274	PF00498	0.406
LIG_FHA_1	288	294	PF00498	0.548
LIG_FHA_1	647	653	PF00498	0.499
LIG_FHA_1	666	672	PF00498	0.515
LIG_FHA_2	254	260	PF00498	0.484
LIG_FHA_2	303	309	PF00498	0.568
LIG_FHA_2	4	10	PF00498	0.890
LIG_FHA_2	556	562	PF00498	0.561
LIG_LIR_Apic_2	189	194	PF02991	0.358
LIG_LIR_Gen_1	466	477	PF02991	0.554
LIG_LIR_Gen_1	492	499	PF02991	0.582
LIG_LIR_Gen_1	510	521	PF02991	0.562
LIG_LIR_Gen_1	544	552	PF02991	0.568
LIG_LIR_Gen_1	665	671	PF02991	0.435
LIG_LIR_Gen_1	716	727	PF02991	0.495
LIG_LIR_Nem_3	207	211	PF02991	0.375
LIG_LIR_Nem_3	341	347	PF02991	0.572
LIG_LIR_Nem_3	438	443	PF02991	0.576
LIG_LIR_Nem_3	459	464	PF02991	0.545
LIG_LIR_Nem_3	466	472	PF02991	0.542
LIG_LIR_Nem_3	475	479	PF02991	0.535
LIG_LIR_Nem_3	510	516	PF02991	0.527
LIG_LIR_Nem_3	544	549	PF02991	0.536
LIG_LIR_Nem_3	665	670	PF02991	0.472
LIG_LIR_Nem_3	684	688	PF02991	0.486
LIG_LIR_Nem_3	698	704	PF02991	0.521
LIG_LIR_Nem_3	716	722	PF02991	0.517
LIG_LIR_Nem_3	772	778	PF02991	0.449
LIG_LIR_Nem_3	786	791	PF02991	0.541
LIG_LIR_Nem_3	9	13	PF02991	0.576
LIG_Pex14_2	616	620	PF04695	0.546
LIG_SH2_CRK	469	473	PF00017	0.543
LIG_SH2_CRK	476	480	PF00017	0.543
LIG_SH2_CRK	524	528	PF00017	0.565
LIG_SH2_CRK	546	550	PF00017	0.548
LIG_SH2_CRK	685	689	PF00017	0.467
LIG_SH2_CRK	701	705	PF00017	0.549
LIG_SH2_CRK	86	90	PF00017	0.859
LIG_SH2_GRB2like	282	285	PF00017	0.596
LIG_SH2_GRB2like	626	629	PF00017	0.512
LIG_SH2_NCK_1	546	550	PF00017	0.563
LIG_SH2_NCK_1	86	90	PF00017	0.592
LIG_SH2_NCK_1	93	97	PF00017	0.585
LIG_SH2_PTP2	738	741	PF00017	0.457
LIG_SH2_SRC	440	443	PF00017	0.560
LIG_SH2_STAP1	217	221	PF00017	0.390
LIG_SH2_STAP1	450	454	PF00017	0.579
LIG_SH2_STAP1	640	644	PF00017	0.415
LIG_SH2_STAP1	70	74	PF00017	0.913
LIG_SH2_STAP1	93	97	PF00017	0.585
LIG_SH2_STAT3	45	48	PF00017	0.573
LIG_SH2_STAT5	247	250	PF00017	0.433
LIG_SH2_STAT5	32	35	PF00017	0.558
LIG_SH2_STAT5	440	443	PF00017	0.551
LIG_SH2_STAT5	45	48	PF00017	0.575
LIG_SH2_STAT5	524	527	PF00017	0.574
LIG_SH2_STAT5	557	560	PF00017	0.526
LIG_SH2_STAT5	56	59	PF00017	0.579
LIG_SH2_STAT5	626	629	PF00017	0.540
LIG_SH2_STAT5	738	741	PF00017	0.406
LIG_SH2_STAT5	756	759	PF00017	0.424
LIG_SH3_3	101	107	PF00018	0.737
LIG_SH3_3	249	255	PF00018	0.391
LIG_SH3_3	324	330	PF00018	0.591
LIG_SH3_3	340	346	PF00018	0.619
LIG_SH3_3	47	53	PF00018	0.783
LIG_SH3_3	658	664	PF00018	0.448
LIG_SH3_3	747	753	PF00018	0.668
LIG_SH3_3	795	801	PF00018	0.530
LIG_SH3_3	92	98	PF00018	0.771
LIG_SH3_5	638	642	PF00018	0.497
LIG_SUMO_SIM_anti_2	726	731	PF11976	0.446
LIG_SUMO_SIM_par_1	654	660	PF11976	0.468
LIG_SUMO_SIM_par_1	686	691	PF11976	0.445
LIG_TRAF2_1	69	72	PF00917	0.901
LIG_TYR_ITIM	196	201	PF00017	0.406
LIG_TYR_ITIM	683	688	PF00017	0.545
LIG_UBA3_1	196	204	PF00899	0.423
LIG_UBA3_1	615	621	PF00899	0.450
LIG_UBA3_1	729	737	PF00899	0.521
LIG_WRPW_2	344	347	PF00400	0.450
MOD_CDK_SPxK_1	527	533	PF00069	0.560
MOD_CK1_1	389	395	PF00069	0.567
MOD_CK2_1	145	151	PF00069	0.558
MOD_CK2_1	253	259	PF00069	0.558
MOD_CK2_1	3	9	PF00069	0.791
MOD_CK2_1	389	395	PF00069	0.539
MOD_CK2_1	555	561	PF00069	0.606
MOD_CK2_1	688	694	PF00069	0.467
MOD_CMANNOS	617	620	PF00535	0.345
MOD_GlcNHglycan	120	123	PF01048	0.635
MOD_GlcNHglycan	319	322	PF01048	0.608
MOD_GlcNHglycan	443	446	PF01048	0.370
MOD_GlcNHglycan	452	455	PF01048	0.412
MOD_GlcNHglycan	480	483	PF01048	0.342
MOD_GlcNHglycan	584	588	PF01048	0.399
MOD_GlcNHglycan	653	656	PF01048	0.564
MOD_GlcNHglycan	707	710	PF01048	0.498
MOD_GSK3_1	489	496	PF00069	0.543
MOD_GSK3_1	522	529	PF00069	0.553
MOD_GSK3_1	665	672	PF00069	0.503
MOD_N-GLC_1	211	216	PF02516	0.392
MOD_N-GLC_1	235	240	PF02516	0.410
MOD_N-GLC_1	3	8	PF02516	0.776
MOD_N-GLC_1	705	710	PF02516	0.467
MOD_NEK2_1	152	157	PF00069	0.572
MOD_NEK2_1	234	239	PF00069	0.349
MOD_NEK2_1	262	267	PF00069	0.464
MOD_NEK2_1	381	386	PF00069	0.566
MOD_NEK2_1	436	441	PF00069	0.579
MOD_NEK2_1	472	477	PF00069	0.552
MOD_NEK2_1	499	504	PF00069	0.610
MOD_NEK2_1	616	621	PF00069	0.545
MOD_NEK2_1	705	710	PF00069	0.487
MOD_NEK2_2	493	498	PF00069	0.633
MOD_NEK2_2	769	774	PF00069	0.629
MOD_OFUCOSY	183	190	PF10250	0.260
MOD_PIKK_1	359	365	PF00454	0.611
MOD_PIKK_1	669	675	PF00454	0.432
MOD_PIKK_1	813	819	PF00454	0.600
MOD_PKA_2	422	428	PF00069	0.530
MOD_PKA_2	712	718	PF00069	0.611
MOD_Plk_1	171	177	PF00069	0.656
MOD_Plk_1	211	217	PF00069	0.400
MOD_Plk_1	234	240	PF00069	0.361
MOD_Plk_1	499	505	PF00069	0.579
MOD_Plk_1	725	731	PF00069	0.442
MOD_Plk_1	769	775	PF00069	0.512
MOD_Plk_2-3	501	507	PF00069	0.594
MOD_Plk_4	171	177	PF00069	0.658
MOD_Plk_4	28	34	PF00069	0.781
MOD_Plk_4	381	387	PF00069	0.549
MOD_Plk_4	436	442	PF00069	0.579
MOD_Plk_4	522	528	PF00069	0.609
MOD_Plk_4	555	561	PF00069	0.566
MOD_Plk_4	725	731	PF00069	0.494
MOD_ProDKin_1	115	121	PF00069	0.682
MOD_ProDKin_1	49	55	PF00069	0.852
MOD_ProDKin_1	527	533	PF00069	0.554
MOD_ProDKin_1	660	666	PF00069	0.472
MOD_SUMO_for_1	789	792	PF00179	0.504
MOD_SUMO_rev_2	500	509	PF00179	0.551
MOD_SUMO_rev_2	561	570	PF00179	0.594
TRG_DiLeu_BaEn_2	256	262	PF01217	0.326
TRG_DiLeu_BaEn_4	15	21	PF01217	0.581
TRG_ENDOCYTIC_2	198	201	PF00928	0.381
TRG_ENDOCYTIC_2	440	443	PF00928	0.572
TRG_ENDOCYTIC_2	469	472	PF00928	0.548
TRG_ENDOCYTIC_2	476	479	PF00928	0.545
TRG_ENDOCYTIC_2	524	527	PF00928	0.547
TRG_ENDOCYTIC_2	546	549	PF00928	0.557
TRG_ENDOCYTIC_2	557	560	PF00928	0.572
TRG_ENDOCYTIC_2	685	688	PF00928	0.445
TRG_ENDOCYTIC_2	701	704	PF00928	0.464
TRG_ENDOCYTIC_2	738	741	PF00928	0.398
TRG_ENDOCYTIC_2	93	96	PF00928	0.881
TRG_ER_diArg_1	126	129	PF00400	0.732
TRG_Pf-PMV_PEXEL_1	11	15	PF00026	0.864
TRG_Pf-PMV_PEXEL_1	287	291	PF00026	0.632

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P8T6	Leptomonas seymouri	34%	100%
A0A0N1I8N2	Leptomonas seymouri	29%	84%
A0A0N1IGQ2	Leptomonas seymouri	32%	100%
A0A0N1ILF1	Leptomonas seymouri	40%	100%
A0A0N1IMH1	Leptomonas seymouri	33%	100%
A0A0N1P9P1	Leptomonas seymouri	40%	96%
A0A0N1PCA9	Leptomonas seymouri	35%	100%
A0A0N1PE91	Leptomonas seymouri	28%	92%
A0A0N1PFI4	Leptomonas seymouri	35%	100%
A0A0S4JLK6	Bodo saltans	27%	100%
A0A0S4JS70	Bodo saltans	25%	100%
A0A0S4KGT2	Bodo saltans	40%	100%
A0A0S4KKP7	Bodo saltans	24%	100%
A0A1X0NJ19	Trypanosomatidae	28%	67%
A0A1X0NJK2	Trypanosomatidae	31%	100%
A0A1X0NJX8	Trypanosomatidae	36%	100%
A0A1X0NKT7	Trypanosomatidae	32%	100%
A0A1X0NKX8	Trypanosomatidae	32%	100%
A0A1X0NMT3	Trypanosomatidae	42%	97%
A0A1X0NW84	Trypanosomatidae	39%	100%
A0A1X0NW85	Trypanosomatidae	39%	100%
A0A1X0NW89	Trypanosomatidae	54%	95%
A0A1X0NWA6	Trypanosomatidae	30%	94%
A0A1X0NWW1	Trypanosomatidae	40%	100%
A0A3Q8IBS3	Leishmania donovani	95%	90%
A0A3Q8IDD4	Leishmania donovani	33%	100%
A0A3Q8IJT4	Leishmania donovani	25%	100%
A0A3S5H5A5	Leishmania donovani	41%	96%
A0A3S5ISG2	Trypanosoma rangeli	31%	100%
A0A3S7WW13	Leishmania donovani	27%	74%
A0A3S7WW18	Leishmania donovani	95%	100%
A0A3S7WW41	Leishmania donovani	35%	100%
A0A3S7WW71	Leishmania donovani	39%	100%
A0A3S7X430	Leishmania donovani	33%	100%
A0A3S7X438	Leishmania donovani	30%	88%
A0A3S7X460	Leishmania donovani	35%	100%
A0A3S7X463	Leishmania donovani	27%	88%
A0A3S7X470	Leishmania donovani	35%	100%
A0A422MYU1	Trypanosoma rangeli	52%	89%
A0A422MYX0	Trypanosoma rangeli	39%	100%
A4H3W4	Leishmania braziliensis	40%	96%
A4HE81	Leishmania braziliensis	34%	100%
A4HJ14	Leishmania braziliensis	34%	100%
A4HJ22	Leishmania braziliensis	32%	87%
A4HJ24	Leishmania braziliensis	34%	100%
A4HS39	Leishmania infantum	41%	96%
A4HYN0	Leishmania infantum	95%	100%
A4HYW1	Leishmania infantum	95%	81%
A4HYW2	Leishmania infantum	39%	100%
A4HYW3	Leishmania infantum	35%	100%
A4HYW4	Leishmania infantum	28%	82%
A4I1J4	Leishmania infantum	33%	100%
A4I6E4	Leishmania infantum	35%	100%
A4I6E6	Leishmania infantum	35%	100%
A4I6F0	Leishmania infantum	30%	88%
A4I6K4	Leishmania infantum	33%	100%
A4I6K5	Leishmania infantum	27%	88%
A4I6K6	Leishmania infantum	25%	100%
C9ZIE7	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	51%	76%
C9ZIE8	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	33%	100%
C9ZIE9	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	38%	100%
C9ZN52	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	34%	100%
C9ZN53	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	31%	96%
C9ZWY4	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	31%	100%
C9ZY36	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	39%	96%
E8NHG6	Leishmania mexicana (strain MHOM/GT/2001/U1103)	35%	100%
E8NHG7	Leishmania mexicana (strain MHOM/GT/2001/U1103)	31%	87%
E8NHG8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	33%	100%
E8NHM2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	33%	100%
E8NHM4	Leishmania mexicana (strain MHOM/GT/2001/U1103)	33%	100%
E8NHM8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	35%	100%
E9AIH1	Leishmania braziliensis	83%	100%
E9AIH3	Leishmania braziliensis	39%	100%
E9AIH4	Leishmania braziliensis	35%	100%
E9AIH6	Leishmania braziliensis	28%	100%
E9AK26	Leishmania mexicana (strain MHOM/GT/2001/U1103)	40%	100%
E9AUQ7	Leishmania mexicana (strain MHOM/GT/2001/U1103)	91%	88%
E9AUQ9	Leishmania mexicana (strain MHOM/GT/2001/U1103)	38%	100%
E9AUR0	Leishmania mexicana (strain MHOM/GT/2001/U1103)	35%	100%
E9AUR1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	28%	100%
E9AXM9	Leishmania mexicana (strain MHOM/GT/2001/U1103)	34%	100%
E9B1J0	Leishmania mexicana (strain MHOM/GT/2001/U1103)	33%	100%
E9B1J1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	26%	88%
E9B1J2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	26%	100%
E9B1J6	Leishmania mexicana (strain MHOM/GT/2001/U1103)	34%	100%
Q4Q6L7	Leishmania major	35%	100%
Q4Q6L9	Leishmania major	35%	100%
Q4Q6M0	Leishmania major	31%	100%
Q4Q6M2	Leishmania major	25%	100%
Q4Q6M3	Leishmania major	27%	100%
Q4Q6M4	Leishmania major	33%	100%
Q4Q9U3	Leishmania major	34%	100%
Q4QCS6	Leishmania major	35%	100%
Q4QCS7	Leishmania major	39%	100%
Q4QCS9	Leishmania major	100%	100%
Q9U0T9	Leishmania major	41%	96%
V5AYJ1	Trypanosoma cruzi	31%	100%
V5B5I4	Trypanosoma cruzi	49%	98%
V5BA05	Trypanosoma cruzi	41%	100%
V5BEL3	Trypanosoma cruzi	36%	100%
V5BII7	Trypanosoma cruzi	29%	67%
V5BN20	Trypanosoma cruzi	35%	100%
V5D9Y2	Trypanosoma cruzi	40%	97%
V5DES7	Trypanosoma cruzi	31%	100%