Calpain-like cysteine peptidase, Clan CA, family C2

Quick info Annotations Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

calpain-like cysteine peptidase, Clan CA, family C2

Species:

Leishmania major

UniProt:

Q4QCS4_LEIMA

TriTrypDb:

LmjF.20.1220 , LMJLV39_200018400 , LMJSD75_200018400

Length:

146

Annotations

LeishMANIAdb interaction annotations

by homology
Contact email: avilan@ula.ve
Publication title: Plasminogen binding proteins in secreted membrane vesicles of Leishmania mexicana
Publication 1st author(s): Figuera
Publication Identifier(s): 23178693
Host organism: -1
Interaction detection method(s): enzymatic linked imunosorbent assay
Interaction type: direct interaction
Identification method participant A: identification by antibody
Identification method participant B: identification by antibody
ID(s) interactor A: E9AUS1
ID(s) interactor B: P00747
Taxid interactor A: Leishmania mexicana
Taxid interactor B: Homo sapiens
Biological role(s) interactor A: unspecified role
Biological role(s) interactor B: unspecified role
Experimental role(s) interactor A: bait

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 2
Forrest at al. (metacyclic)	no	yes: 4
Forrest at al. (procyclic)	no	yes: 4
Silverman et al.	no	yes: 1
Pissara et al.	yes	yes: 23

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	yes	yes: 9

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 18

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 44
NetGPI	no	yes: 0, no: 44

Cellular components
Term	Name	Level	Count
GO:0005654	nucleoplasm	2	1
GO:0005737	cytoplasm	2	1
GO:0031974	membrane-enclosed lumen	2	1
GO:0031981	nuclear lumen	5	1
GO:0032838	plasma membrane bounded cell projection cytoplasm	4	1
GO:0043233	organelle lumen	3	1
GO:0070013	intracellular organelle lumen	4	1
GO:0097014	ciliary plasm	5	1
GO:0097542	ciliary tip	2	1
GO:0099568	cytoplasmic region	3	1
GO:0110165	cellular anatomical entity	1	2
GO:0016020	membrane	2	1
GO:0031090	organelle membrane	3	1
GO:0031253	cell projection membrane	4	1
GO:0060170	ciliary membrane	5	1
GO:0098588	bounding membrane of organelle	4	1
GO:0098590	plasma membrane region	3	1

Expansion

Sequence features

Q4QCS4

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4QCS4

PDB structure(s): 1r75_A , 2fe0_A

Function

Biological processes
Term	Name	Level	Count
GO:0006996	organelle organization	4	1
GO:0009987	cellular process	1	1
GO:0016043	cellular component organization	3	1
GO:0022607	cellular component assembly	4	1
GO:0030030	cell projection organization	4	1
GO:0030031	cell projection assembly	5	1
GO:0044000	movement in host	4	1
GO:0044403	biological process involved in symbiotic interaction	2	1
GO:0044409	entry into host	5	1
GO:0044419	biological process involved in interspecies interaction between organisms	1	1
GO:0044782	cilium organization	5	1
GO:0051701	biological process involved in interaction with host	3	1
GO:0060271	cilium assembly	6	1
GO:0070925	organelle assembly	5	1
GO:0071840	cellular component organization or biogenesis	2	1
GO:0120031	plasma membrane bounded cell projection assembly	6	1
GO:0120036	plasma membrane bounded cell projection organization	5	1

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	1
GO:0016787	hydrolase activity	2	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	136	138	PF00675	0.590
CLV_PCSK_KEX2_1	136	138	PF00082	0.594
CLV_PCSK_SKI1_1	30	34	PF00082	0.421
CLV_PCSK_SKI1_1	63	67	PF00082	0.461
DOC_CYCLIN_RxL_1	60	70	PF00134	0.488
DOC_PP2B_LxvP_1	138	141	PF13499	0.615
DOC_USP7_MATH_1	141	145	PF00917	0.681
DOC_USP7_UBL2_3	23	27	PF12436	0.654
LIG_APCC_ABBA_1	123	128	PF00400	0.437
LIG_BRCT_BRCA1_1	142	146	PF00533	0.494
LIG_FHA_1	5	11	PF00498	0.651
LIG_FHA_2	109	115	PF00498	0.448
LIG_FHA_2	40	46	PF00498	0.448
LIG_FHA_2	76	82	PF00498	0.329
LIG_LIR_Nem_3	119	125	PF02991	0.608
LIG_LIR_Nem_3	31	37	PF02991	0.416
LIG_PDZ_Class_1	141	146	PF00595	0.689
LIG_SH2_CRK	21	25	PF00017	0.725
LIG_SH2_NCK_1	21	25	PF00017	0.763
LIG_SH2_STAP1	109	113	PF00017	0.403
LIG_SH2_STAP1	58	62	PF00017	0.342
LIG_SH2_STAT5	122	125	PF00017	0.607
LIG_SH2_STAT5	21	24	PF00017	0.690
LIG_SH2_STAT5	64	67	PF00017	0.371
LIG_SH3_3	89	95	PF00018	0.328
LIG_SH3_4	23	30	PF00018	0.627
LIG_SH3_4	99	106	PF00018	0.235
LIG_TRAF2_1	42	45	PF00917	0.470
LIG_TRFH_1	123	127	PF08558	0.438
MOD_CK2_1	108	114	PF00069	0.430
MOD_CK2_1	39	45	PF00069	0.398
MOD_CK2_1	75	81	PF00069	0.391
MOD_GlcNHglycan	16	19	PF01048	0.754
MOD_N-GLC_1	4	9	PF02516	0.810
MOD_N-GLC_1	52	57	PF02516	0.394
MOD_NMyristoyl	1	7	PF02799	0.617
MOD_PKA_2	39	45	PF00069	0.379
MOD_SUMO_rev_2	107	113	PF00179	0.383
MOD_SUMO_rev_2	71	80	PF00179	0.421
TRG_DiLeu_BaEn_2	96	102	PF01217	0.386
TRG_DiLeu_BaEn_4	120	126	PF01217	0.439
TRG_DiLeu_BaEn_4	44	50	PF01217	0.261
TRG_ENDOCYTIC_2	122	125	PF00928	0.630
TRG_ENDOCYTIC_2	135	138	PF00928	0.621
TRG_ER_diArg_1	135	137	PF00400	0.610
TRG_PTS1	143	146	PF00515	0.454

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P6Y3	Leptomonas seymouri	40%	100%
A0A0N0P7C3	Leptomonas seymouri	75%	100%
A0A0N1HYV4	Leptomonas seymouri	31%	100%
A0A0N1I5S6	Leptomonas seymouri	43%	95%
A0A0N1I8U4	Leptomonas seymouri	38%	100%
A0A0N1P9P7	Leptomonas seymouri	33%	75%
A0A1X0NW95	Trypanosomatidae	42%	100%
A0A1X0NWH2	Trypanosomatidae	58%	100%
A0A1X0NWV3	Trypanosomatidae	44%	98%
A0A1X0NXS9	Trypanosomatidae	30%	100%
A0A1X0NXT9	Trypanosomatidae	35%	100%
A0A1X0NYV3	Trypanosomatidae	40%	100%
A0A3Q8I9V9	Leishmania donovani	33%	100%
A0A3Q8IBM6	Leishmania donovani	40%	100%
A0A3Q8IBP3	Leishmania donovani	95%	100%
A0A3Q8IKN9	Leishmania donovani	38%	100%
A0A3R7MWC6	Trypanosoma rangeli	39%	100%
A0A422MSY8	Trypanosoma rangeli	41%	87%
A0A422MSZ3	Trypanosoma rangeli	38%	100%
A0A422MUY0	Trypanosoma rangeli	33%	97%
A0A422N035	Trypanosoma rangeli	50%	100%
A0A422N058	Trypanosoma rangeli	29%	95%
A4H7N9	Leishmania braziliensis	33%	100%
A4H7P0	Leishmania braziliensis	33%	100%
A4HW29	Leishmania infantum	33%	100%
A4HYW5	Leishmania infantum	94%	100%
A4HYW6	Leishmania infantum	40%	100%
A4HYX4	Leishmania infantum	38%	100%
C9ZIF2	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	35%	100%
C9ZIF5	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	35%	100%
C9ZIF6	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	36%	100%
E8NHG3	Leishmania mexicana (strain MHOM/GT/2001/U1103)	36%	86%
E9AIH7	Leishmania braziliensis	81%	100%
E9AIH8	Leishmania braziliensis	39%	100%
E9AII3	Leishmania braziliensis	40%	100%
E9AII6	Leishmania braziliensis	44%	100%
E9APT0	Leishmania mexicana (strain MHOM/GT/2001/U1103)	33%	100%
E9AUR2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	88%	100%
E9AUR3	Leishmania mexicana (strain MHOM/GT/2001/U1103)	41%	100%
E9AUS1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	38%	100%
P84150	Leishmania major	41%	100%
Q4QFP8	Leishmania major	33%	100%
Q5SDH5	Leishmania major	37%	100%