tRNA N(3)-methylcytidine methyltransferase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

methyltransferase domain containing protein, putative

Species:

Leishmania major

UniProt:

Q4QCL7_LEIMA

TriTrypDb:

LmjF.21.0020 , LMJLV39_210005000 , LMJSD75_210005000

Length:

346

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Could not find GO cellular_component term for this entry.

Expansion

Sequence features

Q4QCL7

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4QCL7

Function

Biological processes
Term	Name	Level	Count
GO:0001510	RNA methylation	4	12
GO:0006139	nucleobase-containing compound metabolic process	3	12
GO:0006396	RNA processing	6	5
GO:0006399	tRNA metabolic process	7	5
GO:0006400	tRNA modification	6	5
GO:0006725	cellular aromatic compound metabolic process	3	12
GO:0006807	nitrogen compound metabolic process	2	12
GO:0008033	tRNA processing	8	5
GO:0008152	metabolic process	1	12
GO:0009451	RNA modification	5	12
GO:0009987	cellular process	1	12
GO:0016070	RNA metabolic process	5	12
GO:0030488	tRNA methylation	5	5
GO:0032259	methylation	2	12
GO:0034470	ncRNA processing	7	5
GO:0034641	cellular nitrogen compound metabolic process	3	12
GO:0034660	ncRNA metabolic process	6	5
GO:0043170	macromolecule metabolic process	3	12
GO:0043412	macromolecule modification	4	12
GO:0043414	macromolecule methylation	3	12
GO:0044237	cellular metabolic process	2	12
GO:0044238	primary metabolic process	2	12
GO:0044260	obsolete cellular macromolecule metabolic process	3	12
GO:0046483	heterocycle metabolic process	3	12
GO:0071704	organic substance metabolic process	2	12
GO:0090304	nucleic acid metabolic process	4	12
GO:1901360	organic cyclic compound metabolic process	3	12

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	12
GO:0008168	methyltransferase activity	4	12
GO:0016740	transferase activity	2	12
GO:0016741	transferase activity, transferring one-carbon groups	3	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	106	108	PF00675	0.357
CLV_NRD_NRD_1	324	326	PF00675	0.370
CLV_NRD_NRD_1	44	46	PF00675	0.276
CLV_PCSK_KEX2_1	106	108	PF00082	0.357
CLV_PCSK_KEX2_1	190	192	PF00082	0.267
CLV_PCSK_KEX2_1	2	4	PF00082	0.531
CLV_PCSK_PC1ET2_1	190	192	PF00082	0.352
CLV_PCSK_PC1ET2_1	2	4	PF00082	0.601
CLV_PCSK_SKI1_1	196	200	PF00082	0.239
CLV_PCSK_SKI1_1	240	244	PF00082	0.249
DEG_APCC_DBOX_1	162	170	PF00400	0.421
DEG_Nend_UBRbox_1	1	4	PF02207	0.443
DOC_MAPK_DCC_7	163	173	PF00069	0.497
DOC_MAPK_gen_1	193	202	PF00069	0.375
DOC_MAPK_gen_1	2	9	PF00069	0.441
DOC_MAPK_gen_1	325	336	PF00069	0.321
DOC_MAPK_MEF2A_6	129	138	PF00069	0.405
DOC_MAPK_MEF2A_6	2	9	PF00069	0.441
DOC_MAPK_MEF2A_6	216	225	PF00069	0.510
DOC_USP7_MATH_1	172	176	PF00917	0.442
DOC_USP7_MATH_1	208	212	PF00917	0.515
DOC_USP7_UBL2_3	243	247	PF12436	0.507
DOC_USP7_UBL2_3	270	274	PF12436	0.482
DOC_USP7_UBL2_3	326	330	PF12436	0.356
DOC_WW_Pin1_4	106	111	PF00397	0.473
DOC_WW_Pin1_4	130	135	PF00397	0.425
DOC_WW_Pin1_4	141	146	PF00397	0.401
LIG_14-3-3_CanoR_1	163	169	PF00244	0.434
LIG_Actin_WH2_2	228	245	PF00022	0.477
LIG_Actin_WH2_2	86	103	PF00022	0.397
LIG_APCC_ABBA_1	251	256	PF00400	0.437
LIG_Clathr_ClatBox_1	305	309	PF01394	0.316
LIG_deltaCOP1_diTrp_1	73	80	PF00928	0.392
LIG_FHA_1	183	189	PF00498	0.500
LIG_FHA_1	197	203	PF00498	0.410
LIG_FHA_1	284	290	PF00498	0.426
LIG_FHA_1	337	343	PF00498	0.347
LIG_FHA_2	130	136	PF00498	0.534
LIG_FHA_2	178	184	PF00498	0.559
LIG_FHA_2	204	210	PF00498	0.483
LIG_FHA_2	39	45	PF00498	0.401
LIG_LIR_Apic_2	256	260	PF02991	0.262
LIG_LIR_LC3C_4	199	202	PF02991	0.422
LIG_LIR_Nem_3	135	141	PF02991	0.422
LIG_LIR_Nem_3	261	267	PF02991	0.378
LIG_Pex14_1	76	80	PF04695	0.353
LIG_Pex14_2	291	295	PF04695	0.291
LIG_PTAP_UEV_1	183	188	PF05743	0.405
LIG_PTB_Apo_2	334	341	PF02174	0.270
LIG_REV1ctd_RIR_1	262	270	PF16727	0.368
LIG_REV1ctd_RIR_1	288	297	PF16727	0.369
LIG_SH2_NCK_1	257	261	PF00017	0.267
LIG_SH2_SRC	92	95	PF00017	0.385
LIG_SH2_STAT5	160	163	PF00017	0.508
LIG_SH2_STAT5	92	95	PF00017	0.332
LIG_SH3_3	181	187	PF00018	0.505
LIG_SH3_3	4	10	PF00018	0.437
LIG_SH3_3	69	75	PF00018	0.473
LIG_SUMO_SIM_anti_2	218	224	PF11976	0.433
LIG_SUMO_SIM_par_1	169	177	PF11976	0.466
LIG_SUMO_SIM_par_1	198	203	PF11976	0.422
LIG_SUMO_SIM_par_1	304	310	PF11976	0.319
LIG_TRAF2_1	67	70	PF00917	0.566
LIG_TRFH_1	340	344	PF08558	0.368
LIG_UBA3_1	235	243	PF00899	0.415
MOD_CK1_1	146	152	PF00069	0.448
MOD_CK1_1	313	319	PF00069	0.318
MOD_CK2_1	38	44	PF00069	0.397
MOD_CK2_1	64	70	PF00069	0.562
MOD_Cter_Amidation	104	107	PF01082	0.324
MOD_Cter_Amidation	327	330	PF01082	0.380
MOD_GlcNHglycan	145	148	PF01048	0.236
MOD_GlcNHglycan	226	229	PF01048	0.265
MOD_GlcNHglycan	236	239	PF01048	0.271
MOD_GlcNHglycan	301	304	PF01048	0.416
MOD_GSK3_1	146	153	PF00069	0.459
MOD_GSK3_1	173	180	PF00069	0.448
MOD_GSK3_1	224	231	PF00069	0.411
MOD_GSK3_1	294	301	PF00069	0.204
MOD_N-GLC_1	196	201	PF02516	0.222
MOD_N-GLC_1	336	341	PF02516	0.301
MOD_NEK2_1	224	229	PF00069	0.404
MOD_NEK2_1	299	304	PF00069	0.347
MOD_NEK2_1	80	85	PF00069	0.531
MOD_NEK2_1	99	104	PF00069	0.353
MOD_PIKK_1	284	290	PF00454	0.434
MOD_Plk_1	196	202	PF00069	0.512
MOD_Plk_1	208	214	PF00069	0.385
MOD_Plk_1	38	44	PF00069	0.410
MOD_Plk_4	120	126	PF00069	0.415
MOD_Plk_4	156	162	PF00069	0.400
MOD_Plk_4	164	170	PF00069	0.431
MOD_Plk_4	196	202	PF00069	0.419
MOD_ProDKin_1	106	112	PF00069	0.466
MOD_ProDKin_1	130	136	PF00069	0.425
MOD_ProDKin_1	141	147	PF00069	0.401
MOD_SUMO_rev_2	30	38	PF00179	0.360
MOD_SUMO_rev_2	39	48	PF00179	0.318
TRG_DiLeu_BaLyEn_6	95	100	PF01217	0.300
TRG_ER_diArg_1	10	13	PF00400	0.578
TRG_ER_diArg_1	191	194	PF00400	0.454
TRG_NLS_MonoExtC_3	189	195	PF00514	0.431
TRG_NLS_MonoExtN_4	187	194	PF00514	0.431
TRG_Pf-PMV_PEXEL_1	66	70	PF00026	0.581

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P4J3	Leptomonas seymouri	24%	97%
A0A0N1I1R8	Leptomonas seymouri	67%	99%
A0A0S4ISJ6	Bodo saltans	32%	78%
A0A1X0NXB7	Trypanosomatidae	46%	99%
A0A3Q8IDT0	Leishmania donovani	90%	100%
A0A3R7M2M3	Trypanosoma rangeli	25%	100%
A0A3S7X9Q3	Leishmania donovani	24%	89%
A0A422MZQ7	Trypanosoma rangeli	44%	100%
A4HBJ6	Leishmania braziliensis	72%	100%
A4HN30	Leishmania braziliensis	24%	92%
A4HZL8	Leishmania infantum	90%	100%
A4IBP9	Leishmania infantum	24%	89%
A8KBL7	Danio rerio	28%	98%
C9ZYX5	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	26%	100%
D0A1J3	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	45%	100%
E9AFI4	Leishmania major	24%	100%
E9AUW5	Leishmania mexicana (strain MHOM/GT/2001/U1103)	88%	100%
E9B6N9	Leishmania mexicana (strain MHOM/GT/2001/U1103)	25%	93%
O74386	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	28%	100%
Q0P5B2	Bos taurus	28%	92%
Q5RDV8	Pongo abelii	26%	100%
Q5ZHP8	Gallus gallus	27%	94%
Q6AXU8	Rattus norvegicus	28%	100%
Q8BVH9	Mus musculus	27%	100%
Q8TCB7	Homo sapiens	26%	100%
V5BCV4	Trypanosoma cruzi	27%	100%
V5DGW7	Trypanosoma cruzi	44%	100%