ATP-dependent DNA helicase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

DNA repair and recombination helicase protein PIF6, putative

Species:

Leishmania major

UniProt:

Q4QC77_LEIMA

TriTrypDb:

LmjF.21.1190 , LMJLV39_210020000 , LMJSD75_210020200

Length:

786

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	yes	yes: 15

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 14
NetGPI	no	yes: 0, no: 14

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	2
GO:0005657	replication fork	2	2
GO:0005737	cytoplasm	2	15
GO:0043226	organelle	2	2
GO:0043227	membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043231	intracellular membrane-bounded organelle	4	2
GO:0110165	cellular anatomical entity	1	15

Expansion

Sequence features

Q4QC77

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4QC77

Function

Biological processes
Term	Name	Level	Count
GO:0000723	telomere maintenance	5	15
GO:0006139	nucleobase-containing compound metabolic process	3	15
GO:0006259	DNA metabolic process	4	15
GO:0006260	DNA replication	5	2
GO:0006281	DNA repair	5	15
GO:0006310	DNA recombination	5	15
GO:0006725	cellular aromatic compound metabolic process	3	15
GO:0006807	nitrogen compound metabolic process	2	15
GO:0006950	response to stress	2	15
GO:0006974	DNA damage response	4	15
GO:0006996	organelle organization	4	15
GO:0008152	metabolic process	1	15
GO:0009987	cellular process	1	15
GO:0016043	cellular component organization	3	15
GO:0032200	telomere organization	6	15
GO:0032392	DNA geometric change	7	2
GO:0032508	DNA duplex unwinding	8	2
GO:0033554	cellular response to stress	3	15
GO:0034641	cellular nitrogen compound metabolic process	3	15
GO:0043170	macromolecule metabolic process	3	15
GO:0044237	cellular metabolic process	2	15
GO:0044238	primary metabolic process	2	15
GO:0044260	obsolete cellular macromolecule metabolic process	3	15
GO:0046483	heterocycle metabolic process	3	15
GO:0050896	response to stimulus	1	15
GO:0051276	chromosome organization	5	15
GO:0051716	cellular response to stimulus	2	15
GO:0071103	DNA conformation change	6	2
GO:0071704	organic substance metabolic process	2	15
GO:0071840	cellular component organization or biogenesis	2	15
GO:0090304	nucleic acid metabolic process	4	15
GO:1901360	organic cyclic compound metabolic process	3	15

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	15
GO:0000287	magnesium ion binding	5	2
GO:0003678	DNA helicase activity	3	15
GO:0003824	catalytic activity	1	15
GO:0004386	helicase activity	2	15
GO:0005488	binding	1	15
GO:0005524	ATP binding	5	15
GO:0008094	ATP-dependent activity, acting on DNA	2	15
GO:0016462	pyrophosphatase activity	5	15
GO:0016787	hydrolase activity	2	15
GO:0016817	hydrolase activity, acting on acid anhydrides	3	15
GO:0016818	hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides	4	15
GO:0016887	ATP hydrolysis activity	7	15
GO:0017076	purine nucleotide binding	4	15
GO:0017111	ribonucleoside triphosphate phosphatase activity	6	15
GO:0030554	adenyl nucleotide binding	5	15
GO:0032553	ribonucleotide binding	3	15
GO:0032555	purine ribonucleotide binding	4	15
GO:0032559	adenyl ribonucleotide binding	5	15
GO:0035639	purine ribonucleoside triphosphate binding	4	15
GO:0036094	small molecule binding	2	15
GO:0043167	ion binding	2	15
GO:0043168	anion binding	3	15
GO:0043169	cation binding	3	2
GO:0046872	metal ion binding	4	2
GO:0097159	organic cyclic compound binding	2	15
GO:0097367	carbohydrate derivative binding	2	15
GO:0140097	catalytic activity, acting on DNA	3	15
GO:0140640	catalytic activity, acting on a nucleic acid	2	15
GO:0140657	ATP-dependent activity	1	15
GO:1901265	nucleoside phosphate binding	3	15
GO:1901363	heterocyclic compound binding	2	15

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	164	168	PF00656	0.695
CLV_MEL_PAP_1	505	511	PF00089	0.544
CLV_NRD_NRD_1	134	136	PF00675	0.788
CLV_NRD_NRD_1	179	181	PF00675	0.594
CLV_NRD_NRD_1	410	412	PF00675	0.434
CLV_NRD_NRD_1	485	487	PF00675	0.431
CLV_NRD_NRD_1	502	504	PF00675	0.400
CLV_NRD_NRD_1	519	521	PF00675	0.373
CLV_NRD_NRD_1	636	638	PF00675	0.460
CLV_NRD_NRD_1	681	683	PF00675	0.451
CLV_NRD_NRD_1	777	779	PF00675	0.718
CLV_PCSK_FUR_1	132	136	PF00082	0.510
CLV_PCSK_KEX2_1	134	136	PF00082	0.617
CLV_PCSK_KEX2_1	410	412	PF00082	0.437
CLV_PCSK_KEX2_1	485	487	PF00082	0.393
CLV_PCSK_KEX2_1	519	521	PF00082	0.461
CLV_PCSK_KEX2_1	636	638	PF00082	0.405
CLV_PCSK_KEX2_1	779	781	PF00082	0.800
CLV_PCSK_KEX2_1	93	95	PF00082	0.584
CLV_PCSK_PC1ET2_1	779	781	PF00082	0.802
CLV_PCSK_PC1ET2_1	93	95	PF00082	0.621
CLV_PCSK_SKI1_1	296	300	PF00082	0.766
CLV_PCSK_SKI1_1	494	498	PF00082	0.528
CLV_PCSK_SKI1_1	661	665	PF00082	0.390
CLV_PCSK_SKI1_1	666	670	PF00082	0.391
CLV_PCSK_SKI1_1	779	783	PF00082	0.797
DEG_SPOP_SBC_1	404	408	PF00917	0.282
DOC_CYCLIN_yCln2_LP_2	397	403	PF00134	0.282
DOC_MAPK_gen_1	636	643	PF00069	0.371
DOC_MAPK_gen_1	680	689	PF00069	0.436
DOC_MAPK_gen_1	93	100	PF00069	0.542
DOC_MAPK_MEF2A_6	636	643	PF00069	0.340
DOC_MAPK_MEF2A_6	651	659	PF00069	0.365
DOC_PP1_RVXF_1	245	251	PF00149	0.542
DOC_PP2B_LxvP_1	397	400	PF13499	0.282
DOC_PP2B_LxvP_1	401	404	PF13499	0.253
DOC_PP2B_LxvP_1	433	436	PF13499	0.270
DOC_SPAK_OSR1_1	508	512	PF12202	0.534
DOC_USP7_MATH_1	130	134	PF00917	0.761
DOC_USP7_MATH_1	200	204	PF00917	0.700
DOC_USP7_MATH_1	219	223	PF00917	0.710
DOC_USP7_MATH_1	236	240	PF00917	0.654
DOC_USP7_MATH_1	404	408	PF00917	0.323
DOC_USP7_MATH_1	557	561	PF00917	0.481
DOC_USP7_MATH_1	699	703	PF00917	0.418
DOC_USP7_UBL2_3	119	123	PF12436	0.526
DOC_WW_Pin1_4	487	492	PF00397	0.331
DOC_WW_Pin1_4	727	732	PF00397	0.739
LIG_14-3-3_CanoR_1	221	225	PF00244	0.618
LIG_14-3-3_CanoR_1	319	328	PF00244	0.441
LIG_14-3-3_CanoR_1	410	420	PF00244	0.299
LIG_14-3-3_CanoR_1	541	550	PF00244	0.492
LIG_14-3-3_CanoR_1	609	617	PF00244	0.492
LIG_14-3-3_CanoR_1	631	635	PF00244	0.584
LIG_14-3-3_CanoR_1	84	88	PF00244	0.489
LIG_14-3-3_CanoR_1	94	99	PF00244	0.387
LIG_Actin_WH2_2	552	570	PF00022	0.445
LIG_APCC_ABBA_1	22	27	PF00400	0.414
LIG_APCC_ABBAyCdc20_2	21	27	PF00400	0.422
LIG_BIR_II_1	1	5	PF00653	0.624
LIG_BIR_III_4	173	177	PF00653	0.556
LIG_BRCT_BRCA1_1	383	387	PF00533	0.325
LIG_BRCT_BRCA1_1	535	539	PF00533	0.559
LIG_CaM_IQ_9	113	129	PF13499	0.567
LIG_CtBP_PxDLS_1	761	765	PF00389	0.628
LIG_FHA_1	101	107	PF00498	0.388
LIG_FHA_1	186	192	PF00498	0.590
LIG_FHA_1	235	241	PF00498	0.637
LIG_FHA_1	276	282	PF00498	0.407
LIG_FHA_1	315	321	PF00498	0.568
LIG_FHA_1	384	390	PF00498	0.414
LIG_FHA_1	488	494	PF00498	0.381
LIG_FHA_1	512	518	PF00498	0.397
LIG_FHA_1	564	570	PF00498	0.443
LIG_FHA_1	620	626	PF00498	0.578
LIG_FHA_1	750	756	PF00498	0.690
LIG_FHA_1	84	90	PF00498	0.418
LIG_FHA_2	100	106	PF00498	0.443
LIG_FHA_2	180	186	PF00498	0.752
LIG_FHA_2	610	616	PF00498	0.401
LIG_FHA_2	688	694	PF00498	0.415
LIG_LIR_Gen_1	384	394	PF02991	0.390
LIG_LIR_Gen_1	431	438	PF02991	0.271
LIG_LIR_Gen_1	544	555	PF02991	0.484
LIG_LIR_Gen_1	65	75	PF02991	0.545
LIG_LIR_Nem_3	384	390	PF02991	0.334
LIG_LIR_Nem_3	431	435	PF02991	0.342
LIG_LIR_Nem_3	536	542	PF02991	0.389
LIG_LIR_Nem_3	544	550	PF02991	0.364
LIG_LIR_Nem_3	63	69	PF02991	0.421
LIG_LIR_Nem_3	707	711	PF02991	0.430
LIG_MAD2	395	403	PF02301	0.325
LIG_MYND_1	731	735	PF01753	0.499
LIG_SH2_CRK	547	551	PF00017	0.496
LIG_SH2_NCK_1	547	551	PF00017	0.496
LIG_SH2_PTP2	675	678	PF00017	0.365
LIG_SH2_STAT5	675	678	PF00017	0.365
LIG_SH3_3	397	403	PF00018	0.311
LIG_SH3_3	5	11	PF00018	0.521
LIG_SH3_3	556	562	PF00018	0.432
LIG_SH3_3	577	583	PF00018	0.429
LIG_SH3_3	600	606	PF00018	0.474
LIG_SH3_3	729	735	PF00018	0.571
LIG_SH3_3	84	90	PF00018	0.436
LIG_SUMO_SIM_anti_2	372	378	PF11976	0.325
LIG_SUMO_SIM_anti_2	693	698	PF11976	0.396
LIG_SUMO_SIM_par_1	187	192	PF11976	0.510
LIG_SUMO_SIM_par_1	372	378	PF11976	0.261
LIG_SUMO_SIM_par_1	425	431	PF11976	0.310
LIG_SUMO_SIM_par_1	572	578	PF11976	0.389
LIG_SUMO_SIM_par_1	760	766	PF11976	0.536
LIG_TRAF2_1	442	445	PF00917	0.392
MOD_CDK_SPxxK_3	487	494	PF00069	0.328
MOD_CK1_1	213	219	PF00069	0.685
MOD_CK1_1	230	236	PF00069	0.688
MOD_CK1_1	380	386	PF00069	0.434
MOD_CK1_1	560	566	PF00069	0.466
MOD_CK1_1	716	722	PF00069	0.759
MOD_CK1_1	749	755	PF00069	0.710
MOD_CK1_1	772	778	PF00069	0.743
MOD_CK2_1	200	206	PF00069	0.757
MOD_CK2_1	609	615	PF00069	0.444
MOD_CK2_1	664	670	PF00069	0.467
MOD_Cter_Amidation	501	504	PF01082	0.516
MOD_GlcNHglycan	149	152	PF01048	0.672
MOD_GlcNHglycan	15	18	PF01048	0.475
MOD_GlcNHglycan	161	164	PF01048	0.718
MOD_GlcNHglycan	173	177	PF01048	0.718
MOD_GlcNHglycan	191	194	PF01048	0.732
MOD_GlcNHglycan	233	236	PF01048	0.687
MOD_GlcNHglycan	269	272	PF01048	0.394
MOD_GlcNHglycan	341	344	PF01048	0.310
MOD_GlcNHglycan	715	718	PF01048	0.724
MOD_GlcNHglycan	755	758	PF01048	0.734
MOD_GSK3_1	161	168	PF00069	0.652
MOD_GSK3_1	172	179	PF00069	0.730
MOD_GSK3_1	185	192	PF00069	0.739
MOD_GSK3_1	200	207	PF00069	0.580
MOD_GSK3_1	209	216	PF00069	0.626
MOD_GSK3_1	227	234	PF00069	0.733
MOD_GSK3_1	377	384	PF00069	0.456
MOD_GSK3_1	712	719	PF00069	0.655
MOD_GSK3_1	722	729	PF00069	0.460
MOD_GSK3_1	749	756	PF00069	0.677
MOD_N-GLC_1	185	190	PF02516	0.767
MOD_NEK2_1	13	18	PF00069	0.491
MOD_NEK2_1	161	166	PF00069	0.718
MOD_NEK2_1	204	209	PF00069	0.565
MOD_NEK2_1	211	216	PF00069	0.565
MOD_NEK2_1	381	386	PF00069	0.455
MOD_NEK2_1	575	580	PF00069	0.428
MOD_NEK2_1	632	637	PF00069	0.504
MOD_NEK2_1	664	669	PF00069	0.465
MOD_NEK2_1	687	692	PF00069	0.486
MOD_NEK2_2	20	25	PF00069	0.415
MOD_NEK2_2	334	339	PF00069	0.270
MOD_NEK2_2	645	650	PF00069	0.324
MOD_NEK2_2	746	751	PF00069	0.515
MOD_PIKK_1	211	217	PF00454	0.718
MOD_PIKK_1	405	411	PF00454	0.323
MOD_PIKK_1	717	723	PF00454	0.746
MOD_PK_1	94	100	PF00069	0.538
MOD_PKA_2	147	153	PF00069	0.789
MOD_PKA_2	179	185	PF00069	0.630
MOD_PKA_2	220	226	PF00069	0.813
MOD_PKA_2	412	418	PF00069	0.477
MOD_PKA_2	54	60	PF00069	0.461
MOD_PKA_2	608	614	PF00069	0.543
MOD_PKA_2	630	636	PF00069	0.583
MOD_PKA_2	740	746	PF00069	0.723
MOD_PKA_2	772	778	PF00069	0.587
MOD_PKA_2	83	89	PF00069	0.425
MOD_PKB_1	778	786	PF00069	0.771
MOD_Plk_1	185	191	PF00069	0.513
MOD_Plk_1	377	383	PF00069	0.310
MOD_Plk_1	511	517	PF00069	0.501
MOD_Plk_1	557	563	PF00069	0.436
MOD_Plk_1	664	670	PF00069	0.503
MOD_Plk_1	685	691	PF00069	0.384
MOD_Plk_4	200	206	PF00069	0.653
MOD_Plk_4	220	226	PF00069	0.778
MOD_Plk_4	512	518	PF00069	0.415
MOD_Plk_4	560	566	PF00069	0.477
MOD_Plk_4	62	68	PF00069	0.425
MOD_Plk_4	664	670	PF00069	0.510
MOD_Plk_4	83	89	PF00069	0.410
MOD_Plk_4	94	100	PF00069	0.468
MOD_ProDKin_1	487	493	PF00069	0.325
MOD_ProDKin_1	727	733	PF00069	0.743
MOD_SUMO_for_1	781	784	PF00179	0.629
TRG_DiLeu_BaEn_1	512	517	PF01217	0.382
TRG_DiLeu_BaLyEn_6	328	333	PF01217	0.429
TRG_ENDOCYTIC_2	547	550	PF00928	0.448
TRG_ENDOCYTIC_2	675	678	PF00928	0.365
TRG_ER_diArg_1	132	135	PF00400	0.508
TRG_ER_diArg_1	290	293	PF00400	0.603
TRG_ER_diArg_1	410	413	PF00400	0.433
TRG_ER_diArg_1	484	486	PF00400	0.393
TRG_NES_CRM1_1	653	665	PF08389	0.534
TRG_NES_CRM1_1	693	707	PF08389	0.276
TRG_NLS_MonoExtC_3	777	782	PF00514	0.771
TRG_NLS_MonoExtN_4	778	783	PF00514	0.792
TRG_Pf-PMV_PEXEL_1	503	507	PF00026	0.443

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0S4IK87	Bodo saltans	30%	85%
A0A0S4JCS0	Bodo saltans	40%	100%
A0A1X0NZA2	Trypanosomatidae	39%	92%
A0A3Q8IBY0	Leishmania donovani	94%	100%
A0A3R7MKN4	Trypanosoma rangeli	29%	82%
A0A422NAK1	Trypanosoma rangeli	49%	100%
A4HBX5	Leishmania braziliensis	81%	100%
A4HZ95	Leishmania infantum	93%	100%
C9ZUJ0	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	30%	79%
D0A177	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	44%	99%
E9AVA3	Leishmania mexicana (strain MHOM/GT/2001/U1103)	90%	100%
Q38CE9	Trypanosoma brucei brucei (strain 927/4 GUTat10.1)	44%	99%
Q57YG0	Trypanosoma brucei brucei (strain 927/4 GUTat10.1)	30%	79%
Q9UUA2	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	29%	98%
V5B8C2	Trypanosoma cruzi	29%	81%
V5BE68	Trypanosoma cruzi	49%	100%