EF-hand domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

hypothetical protein, conserved

Species:

Leishmania major

UniProt:

Q4QBQ5_LEIMA

TriTrypDb:

LmjF.22.0920 , LMJLV39_220014400 , LMJSD75_220014900

Length:

924

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 1, no: 5
NetGPI	no	yes: 0, no: 6

Cellular components
Term	Name	Level	Count
GO:0032991	protein-containing complex	1	2
GO:0034702	monoatomic ion channel complex	4	2
GO:0034703	cation channel complex	5	2
GO:0034704	calcium channel complex	6	2
GO:0098796	membrane protein complex	2	2
GO:0098798	mitochondrial protein-containing complex	2	2
GO:0098800	inner mitochondrial membrane protein complex	3	2
GO:1902495	transmembrane transporter complex	3	2
GO:1990246	uniplex complex	4	2
GO:1990351	transporter complex	2	2

Expansion

Sequence features

Q4QBQ5

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4QBQ5

Function

Biological processes
Term	Name	Level	Count
GO:0006810	transport	3	7
GO:0006811	monoatomic ion transport	4	7
GO:0006812	monoatomic cation transport	5	7
GO:0006816	calcium ion transport	7	7
GO:0006851	mitochondrial calcium ion transmembrane transport	4	7
GO:0006873	intracellular monoatomic ion homeostasis	4	2
GO:0006874	intracellular calcium ion homeostasis	7	2
GO:0006875	obsolete intracellular metal ion homeostasis	6	2
GO:0009987	cellular process	1	7
GO:0019725	cellular homeostasis	2	2
GO:0030001	metal ion transport	6	7
GO:0030003	intracellular monoatomic cation homeostasis	5	2
GO:0034220	monoatomic ion transmembrane transport	3	7
GO:0036444	calcium import into the mitochondrion	5	2
GO:0042592	homeostatic process	3	2
GO:0048878	chemical homeostasis	4	2
GO:0050801	monoatomic ion homeostasis	5	2
GO:0051179	localization	1	7
GO:0051234	establishment of localization	2	7
GO:0051560	mitochondrial calcium ion homeostasis	8	2
GO:0055065	obsolete metal ion homeostasis	7	2
GO:0055074	calcium ion homeostasis	8	2
GO:0055080	monoatomic cation homeostasis	6	2
GO:0055082	intracellular chemical homeostasis	3	2
GO:0055085	transmembrane transport	2	7
GO:0065007	biological regulation	1	2
GO:0065008	regulation of biological quality	2	2
GO:0070588	calcium ion transmembrane transport	6	7
GO:0072503	obsolete cellular divalent inorganic cation homeostasis	6	2
GO:0072507	obsolete divalent inorganic cation homeostasis	7	2
GO:0098655	monoatomic cation transmembrane transport	4	7
GO:0098660	inorganic ion transmembrane transport	4	7
GO:0098662	inorganic cation transmembrane transport	5	7
GO:0098771	inorganic ion homeostasis	6	2
GO:1990542	mitochondrial transmembrane transport	3	7

Molecular functions
Term	Name	Level	Count
GO:0005488	binding	1	7
GO:0005509	calcium ion binding	5	7
GO:0043167	ion binding	2	7
GO:0043169	cation binding	3	7
GO:0046872	metal ion binding	4	7

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	102	106	PF00656	0.624
CLV_C14_Caspase3-7	129	133	PF00656	0.418
CLV_C14_Caspase3-7	290	294	PF00656	0.623
CLV_C14_Caspase3-7	814	818	PF00656	0.718
CLV_C14_Caspase3-7	877	881	PF00656	0.629
CLV_NRD_NRD_1	269	271	PF00675	0.651
CLV_NRD_NRD_1	466	468	PF00675	0.814
CLV_NRD_NRD_1	568	570	PF00675	0.588
CLV_NRD_NRD_1	576	578	PF00675	0.518
CLV_NRD_NRD_1	675	677	PF00675	0.577
CLV_NRD_NRD_1	683	685	PF00675	0.541
CLV_NRD_NRD_1	87	89	PF00675	0.675
CLV_PCSK_KEX2_1	20	22	PF00082	0.496
CLV_PCSK_KEX2_1	269	271	PF00082	0.651
CLV_PCSK_KEX2_1	366	368	PF00082	0.488
CLV_PCSK_KEX2_1	466	468	PF00082	0.814
CLV_PCSK_KEX2_1	576	578	PF00082	0.558
CLV_PCSK_KEX2_1	675	677	PF00082	0.577
CLV_PCSK_KEX2_1	683	685	PF00082	0.541
CLV_PCSK_KEX2_1	87	89	PF00082	0.675
CLV_PCSK_KEX2_1	909	911	PF00082	0.671
CLV_PCSK_PC1ET2_1	20	22	PF00082	0.496
CLV_PCSK_PC1ET2_1	366	368	PF00082	0.485
CLV_PCSK_PC1ET2_1	909	911	PF00082	0.671
CLV_PCSK_PC7_1	679	685	PF00082	0.555
CLV_PCSK_SKI1_1	253	257	PF00082	0.449
CLV_PCSK_SKI1_1	366	370	PF00082	0.483
CLV_PCSK_SKI1_1	48	52	PF00082	0.769
CLV_PCSK_SKI1_1	495	499	PF00082	0.437
CLV_PCSK_SKI1_1	576	580	PF00082	0.731
CLV_PCSK_SKI1_1	581	585	PF00082	0.601
CLV_PCSK_SKI1_1	675	679	PF00082	0.509
CLV_PCSK_SKI1_1	684	688	PF00082	0.601
CLV_PCSK_SKI1_1	728	732	PF00082	0.514
CLV_PCSK_SKI1_1	832	836	PF00082	0.591
CLV_PCSK_SKI1_1	867	871	PF00082	0.609
CLV_PCSK_SKI1_1	894	898	PF00082	0.542
CLV_Separin_Metazoa	492	496	PF03568	0.555
CLV_Separin_Metazoa	761	765	PF03568	0.539
DEG_APCC_DBOX_1	575	583	PF00400	0.608
DEG_APCC_DBOX_1	649	657	PF00400	0.465
DEG_APCC_DBOX_1	727	735	PF00400	0.548
DEG_Nend_UBRbox_4	1	3	PF02207	0.534
DEG_SCF_FBW7_1	582	588	PF00400	0.713
DOC_CDC14_PxL_1	636	644	PF14671	0.471
DOC_CDC14_PxL_1	856	864	PF14671	0.411
DOC_CKS1_1	358	363	PF01111	0.590
DOC_CKS1_1	582	587	PF01111	0.711
DOC_CYCLIN_RxL_1	570	580	PF00134	0.638
DOC_CYCLIN_RxL_1	867	877	PF00134	0.603
DOC_CYCLIN_yCln2_LP_2	493	499	PF00134	0.584
DOC_MAPK_gen_1	19	28	PF00069	0.492
DOC_MAPK_gen_1	479	489	PF00069	0.507
DOC_MAPK_MEF2A_6	482	489	PF00069	0.437
DOC_MAPK_MEF2A_6	722	731	PF00069	0.597
DOC_MAPK_NFAT4_5	482	490	PF00069	0.429
DOC_MAPK_RevD_3	255	270	PF00069	0.557
DOC_PP1_RVXF_1	493	500	PF00149	0.446
DOC_PP1_RVXF_1	652	659	PF00149	0.468
DOC_PP2B_LxvP_1	256	259	PF13499	0.475
DOC_PP4_FxxP_1	905	908	PF00568	0.538
DOC_USP7_MATH_1	169	173	PF00917	0.723
DOC_USP7_MATH_1	276	280	PF00917	0.714
DOC_USP7_MATH_1	404	408	PF00917	0.567
DOC_USP7_MATH_1	409	413	PF00917	0.581
DOC_USP7_MATH_1	42	46	PF00917	0.562
DOC_USP7_MATH_1	424	428	PF00917	0.761
DOC_USP7_MATH_1	455	459	PF00917	0.826
DOC_USP7_MATH_1	460	464	PF00917	0.710
DOC_USP7_MATH_1	560	564	PF00917	0.637
DOC_USP7_MATH_1	585	589	PF00917	0.773
DOC_USP7_MATH_1	59	63	PF00917	0.679
DOC_USP7_MATH_1	596	600	PF00917	0.595
DOC_USP7_MATH_1	602	606	PF00917	0.477
DOC_USP7_MATH_1	616	620	PF00917	0.665
DOC_USP7_MATH_1	818	822	PF00917	0.545
DOC_USP7_MATH_1	865	869	PF00917	0.571
DOC_WW_Pin1_4	165	170	PF00397	0.741
DOC_WW_Pin1_4	283	288	PF00397	0.683
DOC_WW_Pin1_4	297	302	PF00397	0.461
DOC_WW_Pin1_4	357	362	PF00397	0.577
DOC_WW_Pin1_4	4	9	PF00397	0.508
DOC_WW_Pin1_4	417	422	PF00397	0.761
DOC_WW_Pin1_4	426	431	PF00397	0.650
DOC_WW_Pin1_4	443	448	PF00397	0.454
DOC_WW_Pin1_4	542	547	PF00397	0.602
DOC_WW_Pin1_4	581	586	PF00397	0.748
DOC_WW_Pin1_4	70	75	PF00397	0.741
DOC_WW_Pin1_4	803	808	PF00397	0.795
DOC_WW_Pin1_4	880	885	PF00397	0.460
DOC_WW_Pin1_4	94	99	PF00397	0.647
LIG_14-3-3_CanoR_1	109	114	PF00244	0.624
LIG_14-3-3_CanoR_1	314	320	PF00244	0.592
LIG_14-3-3_CanoR_1	367	372	PF00244	0.496
LIG_14-3-3_CanoR_1	379	385	PF00244	0.287
LIG_14-3-3_CanoR_1	467	473	PF00244	0.632
LIG_14-3-3_CanoR_1	554	562	PF00244	0.704
LIG_14-3-3_CanoR_1	620	626	PF00244	0.553
LIG_14-3-3_CanoR_1	635	640	PF00244	0.329
LIG_14-3-3_CanoR_1	683	687	PF00244	0.614
LIG_14-3-3_CanoR_1	764	768	PF00244	0.599
LIG_14-3-3_CanoR_1	87	93	PF00244	0.679
LIG_14-3-3_CanoR_1	889	894	PF00244	0.454
LIG_Actin_WH2_2	351	368	PF00022	0.453
LIG_Actin_WH2_2	639	656	PF00022	0.455
LIG_Actin_WH2_2	727	745	PF00022	0.644
LIG_Actin_WH2_2	756	771	PF00022	0.536
LIG_BIR_III_2	732	736	PF00653	0.629
LIG_BRCT_BRCA1_1	287	291	PF00533	0.800
LIG_deltaCOP1_diTrp_1	382	385	PF00928	0.562
LIG_FHA_1	115	121	PF00498	0.656
LIG_FHA_1	133	139	PF00498	0.369
LIG_FHA_1	159	165	PF00498	0.662
LIG_FHA_1	207	213	PF00498	0.661
LIG_FHA_1	215	221	PF00498	0.591
LIG_FHA_1	389	395	PF00498	0.462
LIG_FHA_1	451	457	PF00498	0.752
LIG_FHA_1	598	604	PF00498	0.720
LIG_FHA_1	605	611	PF00498	0.602
LIG_FHA_1	664	670	PF00498	0.609
LIG_FHA_1	754	760	PF00498	0.556
LIG_FHA_1	787	793	PF00498	0.702
LIG_FHA_2	158	164	PF00498	0.657
LIG_FHA_2	197	203	PF00498	0.731
LIG_FHA_2	288	294	PF00498	0.653
LIG_FHA_2	511	517	PF00498	0.746
LIG_FHA_2	812	818	PF00498	0.719
LIG_Integrin_RGD_1	679	681	PF01839	0.583
LIG_IRF3_LxIS_1	485	490	PF10401	0.399
LIG_LIR_Gen_1	350	358	PF02991	0.413
LIG_LIR_Gen_1	382	393	PF02991	0.457
LIG_LIR_Gen_1	63	74	PF02991	0.738
LIG_LIR_Gen_1	845	854	PF02991	0.582
LIG_LIR_Gen_1	886	893	PF02991	0.502
LIG_LIR_Nem_3	229	234	PF02991	0.491
LIG_LIR_Nem_3	350	354	PF02991	0.408
LIG_LIR_Nem_3	382	388	PF02991	0.570
LIG_LIR_Nem_3	573	578	PF02991	0.685
LIG_LIR_Nem_3	63	69	PF02991	0.734
LIG_LIR_Nem_3	638	642	PF02991	0.408
LIG_LIR_Nem_3	706	710	PF02991	0.493
LIG_LIR_Nem_3	845	849	PF02991	0.469
LIG_LIR_Nem_3	868	873	PF02991	0.598
LIG_LIR_Nem_3	886	890	PF02991	0.366
LIG_LYPXL_yS_3	639	642	PF13949	0.475
LIG_MYND_1	439	443	PF01753	0.673
LIG_Pex14_2	331	335	PF04695	0.385
LIG_Pex14_2	351	355	PF04695	0.384
LIG_SH2_STAP1	348	352	PF00017	0.433
LIG_SH2_STAT3	529	532	PF00017	0.502
LIG_SH2_STAT3	918	921	PF00017	0.494
LIG_SH2_STAT5	648	651	PF00017	0.457
LIG_SH2_STAT5	66	69	PF00017	0.682
LIG_SH2_STAT5	918	921	PF00017	0.494
LIG_SH3_3	3	9	PF00018	0.510
LIG_SH3_3	36	42	PF00018	0.489
LIG_SH3_3	579	585	PF00018	0.699
LIG_SH3_3	634	640	PF00018	0.445
LIG_SH3_3	71	77	PF00018	0.661
LIG_SH3_3	732	738	PF00018	0.516
LIG_SH3_3	804	810	PF00018	0.724
LIG_SUMO_SIM_par_1	243	249	PF11976	0.466
LIG_SUMO_SIM_par_1	254	261	PF11976	0.489
LIG_SUMO_SIM_par_1	390	395	PF11976	0.479
LIG_SUMO_SIM_par_1	599	605	PF11976	0.640
LIG_SUMO_SIM_par_1	607	613	PF11976	0.535
LIG_SUMO_SIM_par_1	666	673	PF11976	0.485
LIG_TRAF2_1	627	630	PF00917	0.471
LIG_TRAF2_1	774	777	PF00917	0.521
LIG_TYR_ITIM	637	642	PF00017	0.468
LIG_WRC_WIRS_1	354	359	PF05994	0.451
MOD_CK1_1	112	118	PF00069	0.678
MOD_CK1_1	165	171	PF00069	0.715
MOD_CK1_1	206	212	PF00069	0.654
MOD_CK1_1	4	10	PF00069	0.507
MOD_CK1_1	420	426	PF00069	0.691
MOD_CK1_1	471	477	PF00069	0.737
MOD_CK1_1	524	530	PF00069	0.699
MOD_CK1_1	619	625	PF00069	0.511
MOD_CK1_1	70	76	PF00069	0.699
MOD_CK1_1	806	812	PF00069	0.717
MOD_CK2_1	112	118	PF00069	0.659
MOD_CK2_1	157	163	PF00069	0.625
MOD_CK2_1	196	202	PF00069	0.739
MOD_CK2_1	378	384	PF00069	0.441
MOD_CK2_1	471	477	PF00069	0.680
MOD_CK2_1	820	826	PF00069	0.641
MOD_CK2_1	860	866	PF00069	0.507
MOD_CK2_1	880	886	PF00069	0.283
MOD_CMANNOS	568	571	PF00535	0.738
MOD_GlcNHglycan	184	187	PF01048	0.629
MOD_GlcNHglycan	205	208	PF01048	0.645
MOD_GlcNHglycan	272	275	PF01048	0.762
MOD_GlcNHglycan	278	281	PF01048	0.706
MOD_GlcNHglycan	406	409	PF01048	0.725
MOD_GlcNHglycan	411	414	PF01048	0.674
MOD_GlcNHglycan	422	425	PF01048	0.562
MOD_GlcNHglycan	426	429	PF01048	0.673
MOD_GlcNHglycan	457	460	PF01048	0.817
MOD_GlcNHglycan	53	56	PF01048	0.664
MOD_GlcNHglycan	539	542	PF01048	0.664
MOD_GlcNHglycan	564	567	PF01048	0.649
MOD_GlcNHglycan	88	91	PF01048	0.799
MOD_GlcNHglycan	94	97	PF01048	0.696
MOD_GSK3_1	112	119	PF00069	0.547
MOD_GSK3_1	158	165	PF00069	0.682
MOD_GSK3_1	270	277	PF00069	0.770
MOD_GSK3_1	283	290	PF00069	0.586
MOD_GSK3_1	310	317	PF00069	0.553
MOD_GSK3_1	353	360	PF00069	0.450
MOD_GSK3_1	4	11	PF00069	0.508
MOD_GSK3_1	420	427	PF00069	0.787
MOD_GSK3_1	461	468	PF00069	0.713
MOD_GSK3_1	521	528	PF00069	0.547
MOD_GSK3_1	577	584	PF00069	0.689
MOD_GSK3_1	612	619	PF00069	0.623
MOD_GSK3_1	663	670	PF00069	0.544
MOD_GSK3_1	786	793	PF00069	0.758
MOD_GSK3_1	808	815	PF00069	0.563
MOD_GSK3_1	876	883	PF00069	0.521
MOD_GSK3_1	88	95	PF00069	0.765
MOD_N-GLC_1	157	162	PF02516	0.700
MOD_N-GLC_1	542	547	PF02516	0.668
MOD_NEK2_1	1	6	PF00069	0.523
MOD_NEK2_1	131	136	PF00069	0.581
MOD_NEK2_1	139	144	PF00069	0.501
MOD_NEK2_1	205	210	PF00069	0.687
MOD_NEK2_1	388	393	PF00069	0.508
MOD_NEK2_1	51	56	PF00069	0.754
MOD_NEK2_1	521	526	PF00069	0.589
MOD_NEK2_1	768	773	PF00069	0.582
MOD_NEK2_1	83	88	PF00069	0.748
MOD_NEK2_1	834	839	PF00069	0.537
MOD_NEK2_2	738	743	PF00069	0.555
MOD_NEK2_2	865	870	PF00069	0.518
MOD_OFUCOSY	589	594	PF10250	0.760
MOD_PIKK_1	206	212	PF00454	0.678
MOD_PIKK_1	554	560	PF00454	0.706
MOD_PIKK_1	619	625	PF00454	0.545
MOD_PIKK_1	648	654	PF00454	0.444
MOD_PIKK_1	753	759	PF00454	0.659
MOD_PIKK_1	792	798	PF00454	0.806
MOD_PK_1	109	115	PF00069	0.618
MOD_PK_1	367	373	PF00069	0.599
MOD_PKA_1	20	26	PF00069	0.490
MOD_PKA_2	20	26	PF00069	0.490
MOD_PKA_2	378	384	PF00069	0.465
MOD_PKA_2	465	471	PF00069	0.664
MOD_PKA_2	619	625	PF00069	0.555
MOD_PKA_2	682	688	PF00069	0.630
MOD_PKA_2	763	769	PF00069	0.611
MOD_PKA_2	839	845	PF00069	0.492
MOD_PKA_2	86	92	PF00069	0.756
MOD_Plk_1	214	220	PF00069	0.590
MOD_Plk_1	629	635	PF00069	0.499
MOD_Plk_1	700	706	PF00069	0.581
MOD_Plk_1	738	744	PF00069	0.559
MOD_Plk_1	865	871	PF00069	0.569
MOD_Plk_2-3	196	202	PF00069	0.723
MOD_Plk_2-3	820	826	PF00069	0.643
MOD_Plk_4	119	125	PF00069	0.640
MOD_Plk_4	208	214	PF00069	0.674
MOD_Plk_4	315	321	PF00069	0.487
MOD_Plk_4	388	394	PF00069	0.431
MOD_Plk_4	521	527	PF00069	0.606
MOD_Plk_4	629	635	PF00069	0.440
MOD_Plk_4	763	769	PF00069	0.523
MOD_Plk_4	834	840	PF00069	0.534
MOD_ProDKin_1	165	171	PF00069	0.742
MOD_ProDKin_1	283	289	PF00069	0.683
MOD_ProDKin_1	297	303	PF00069	0.449
MOD_ProDKin_1	357	363	PF00069	0.584
MOD_ProDKin_1	4	10	PF00069	0.507
MOD_ProDKin_1	417	423	PF00069	0.762
MOD_ProDKin_1	426	432	PF00069	0.650
MOD_ProDKin_1	443	449	PF00069	0.453
MOD_ProDKin_1	542	548	PF00069	0.604
MOD_ProDKin_1	581	587	PF00069	0.749
MOD_ProDKin_1	70	76	PF00069	0.738
MOD_ProDKin_1	803	809	PF00069	0.790
MOD_ProDKin_1	880	886	PF00069	0.453
MOD_ProDKin_1	94	100	PF00069	0.644
MOD_SUMO_for_1	686	689	PF00179	0.673
MOD_SUMO_rev_2	817	824	PF00179	0.738
TRG_DiLeu_BaEn_1	384	389	PF01217	0.451
TRG_DiLeu_BaLyEn_6	493	498	PF01217	0.460
TRG_DiLeu_BaLyEn_6	574	579	PF01217	0.484
TRG_DiLeu_BaLyEn_6	673	678	PF01217	0.595
TRG_ENDOCYTIC_2	348	351	PF00928	0.406
TRG_ENDOCYTIC_2	639	642	PF00928	0.404
TRG_ENDOCYTIC_2	66	69	PF00928	0.783
TRG_ENDOCYTIC_2	710	713	PF00928	0.469
TRG_ER_diArg_1	268	270	PF00400	0.649
TRG_ER_diArg_1	575	577	PF00400	0.486
TRG_ER_diArg_1	675	677	PF00400	0.530
TRG_ER_diArg_1	682	684	PF00400	0.407
TRG_NES_CRM1_1	363	376	PF08389	0.499
TRG_Pf-PMV_PEXEL_1	136	140	PF00026	0.671
TRG_Pf-PMV_PEXEL_1	676	681	PF00026	0.628
TRG_Pf-PMV_PEXEL_1	871	875	PF00026	0.498

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P6M5	Leptomonas seymouri	38%	100%
A0A3S7WX62	Leishmania donovani	88%	100%
A4HCE3	Leishmania braziliensis	62%	100%
A4HZW6	Leishmania infantum	89%	100%
E9AVS4	Leishmania mexicana (strain MHOM/GT/2001/U1103)	80%	100%