Putative Serine-threonin protein phosphatase

Quick info Annotations Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

kinetoplastid-specific phospho-protein phosphatase, putative

Species:

Leishmania major

UniProt:

Q4QBJ8_LEIMA

TriTrypDb:

LmjF.22.1490 , LMJLV39_220020600 , LMJSD75_220021300

Length:

361

Annotations

LeishMANIAdb annotations

A metalloenzyme with the catalytic domain facing outwards. Related to plant Shewanella-like protein phosphatases. Kinetoplastids have multiple copies of these genes but probably from a very ancient gene duplication.. The cluster might merge two separate, very distantly related groups.

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	yes	yes: 12, no: 1
NetGPI	no	yes: 0, no: 13

Cellular components
Term	Name	Level	Count
GO:0016020	membrane	2	2
GO:0110165	cellular anatomical entity	1	2

Expansion

Sequence features

Q4QBJ8

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4QBJ8

Function

Could not find GO biological_process term for this entry.

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	14
GO:0016787	hydrolase activity	2	14

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	237	239	PF00675	0.372
CLV_NRD_NRD_1	31	33	PF00675	0.519
CLV_PCSK_SKI1_1	216	220	PF00082	0.364
CLV_PCSK_SKI1_1	253	257	PF00082	0.399
DEG_APCC_DBOX_1	252	260	PF00400	0.366
DEG_APCC_DBOX_1	92	100	PF00400	0.372
DEG_Nend_UBRbox_1	1	4	PF02207	0.603
DEG_SCF_FBW7_1	266	273	PF00400	0.323
DOC_MAPK_gen_1	29	37	PF00069	0.527
DOC_MAPK_MEF2A_6	10	19	PF00069	0.566
DOC_MAPK_MEF2A_6	298	306	PF00069	0.475
DOC_USP7_MATH_1	270	274	PF00917	0.441
DOC_USP7_UBL2_3	308	312	PF12436	0.294
DOC_WW_Pin1_4	266	271	PF00397	0.323
LIG_14-3-3_CanoR_1	225	229	PF00244	0.443
LIG_14-3-3_CanoR_1	93	97	PF00244	0.267
LIG_Actin_WH2_2	204	222	PF00022	0.366
LIG_BRCT_BRCA1_1	317	321	PF00533	0.503
LIG_eIF4E_1	12	18	PF01652	0.568
LIG_FHA_1	260	266	PF00498	0.416
LIG_FHA_1	92	98	PF00498	0.337
LIG_FHA_2	267	273	PF00498	0.381
LIG_FHA_2	334	340	PF00498	0.642
LIG_IRF3_LxIS_1	18	25	PF10401	0.592
LIG_LIR_Gen_1	356	361	PF02991	0.710
LIG_LIR_Gen_1	51	60	PF02991	0.426
LIG_LIR_Nem_3	172	178	PF02991	0.321
LIG_LIR_Nem_3	303	309	PF02991	0.450
LIG_LIR_Nem_3	356	361	PF02991	0.689
LIG_LIR_Nem_3	51	56	PF02991	0.373
LIG_PDZ_Class_3	356	361	PF00595	0.678
LIG_Pex14_2	224	228	PF04695	0.439
LIG_SH2_CRK	12	16	PF00017	0.613
LIG_SH2_CRK	148	152	PF00017	0.396
LIG_SH2_CRK	296	300	PF00017	0.351
LIG_SH2_GRB2like	150	153	PF00017	0.396
LIG_SH2_NCK_1	296	300	PF00017	0.472
LIG_SH2_PTP2	20	23	PF00017	0.368
LIG_SH2_STAP1	296	300	PF00017	0.499
LIG_SH2_STAT5	150	153	PF00017	0.295
LIG_SH2_STAT5	178	181	PF00017	0.341
LIG_SH2_STAT5	20	23	PF00017	0.506
LIG_SUMO_SIM_anti_2	130	137	PF11976	0.357
LIG_SUMO_SIM_par_1	20	25	PF11976	0.605
LIG_SUMO_SIM_par_1	283	288	PF11976	0.328
LIG_SUMO_SIM_par_1	39	45	PF11976	0.318
LIG_TRAF2_1	118	121	PF00917	0.426
LIG_TRAF2_1	269	272	PF00917	0.426
LIG_TYR_ITIM	18	23	PF00017	0.586
LIG_WW_3	76	80	PF00397	0.443
MOD_CK1_1	84	90	PF00069	0.231
MOD_CK1_1	91	97	PF00069	0.231
MOD_CK2_1	115	121	PF00069	0.396
MOD_CK2_1	125	131	PF00069	0.396
MOD_CK2_1	266	272	PF00069	0.426
MOD_GlcNHglycan	125	128	PF01048	0.429
MOD_GlcNHglycan	231	234	PF01048	0.377
MOD_GlcNHglycan	272	275	PF01048	0.476
MOD_GlcNHglycan	317	320	PF01048	0.509
MOD_GlcNHglycan	330	333	PF01048	0.627
MOD_GlcNHglycan	339	344	PF01048	0.661
MOD_GlcNHglycan	70	73	PF01048	0.446
MOD_GSK3_1	224	231	PF00069	0.420
MOD_GSK3_1	243	250	PF00069	0.438
MOD_GSK3_1	266	273	PF00069	0.333
MOD_GSK3_1	311	318	PF00069	0.518
MOD_GSK3_1	339	346	PF00069	0.652
MOD_GSK3_1	80	87	PF00069	0.386
MOD_GSK3_1	88	95	PF00069	0.317
MOD_N-GLC_1	259	264	PF02516	0.402
MOD_N-GLC_1	87	92	PF02516	0.394
MOD_NEK2_1	169	174	PF00069	0.398
MOD_NEK2_1	22	27	PF00069	0.567
MOD_NEK2_1	224	229	PF00069	0.416
MOD_NEK2_1	259	264	PF00069	0.351
MOD_NEK2_1	304	309	PF00069	0.372
MOD_NEK2_1	333	338	PF00069	0.640
MOD_NEK2_1	344	349	PF00069	0.633
MOD_PIKK_1	125	131	PF00454	0.413
MOD_PKA_2	224	230	PF00069	0.426
MOD_PKA_2	92	98	PF00069	0.345
MOD_Plk_1	199	205	PF00069	0.410
MOD_Plk_1	259	265	PF00069	0.376
MOD_Plk_1	344	350	PF00069	0.655
MOD_Plk_1	63	69	PF00069	0.249
MOD_Plk_2-3	243	249	PF00069	0.288
MOD_Plk_4	224	230	PF00069	0.451
MOD_Plk_4	317	323	PF00069	0.531
MOD_Plk_4	92	98	PF00069	0.324
MOD_ProDKin_1	266	272	PF00069	0.323
MOD_SUMO_rev_2	25	35	PF00179	0.578
TRG_DiLeu_BaEn_1	131	136	PF01217	0.352
TRG_DiLeu_BaEn_2	189	195	PF01217	0.420
TRG_ENDOCYTIC_2	12	15	PF00928	0.553
TRG_ENDOCYTIC_2	148	151	PF00928	0.396
TRG_ENDOCYTIC_2	156	159	PF00928	0.396
TRG_ENDOCYTIC_2	20	23	PF00928	0.554
TRG_ENDOCYTIC_2	296	299	PF00928	0.353
TRG_Pf-PMV_PEXEL_1	32	36	PF00026	0.495
TRG_Pf-PMV_PEXEL_1	324	328	PF00026	0.494

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I3V2	Leptomonas seymouri	29%	97%
A0A0N1P9R1	Leptomonas seymouri	57%	100%
A0A0S4JBT9	Bodo saltans	39%	93%
A0A0S4JMN0	Bodo saltans	33%	89%
A0A0S4KJG1	Bodo saltans	27%	97%
A0A1X0NU01	Trypanosomatidae	45%	97%
A0A1X0NZX7	Trypanosomatidae	31%	81%
A0A1X0P2G6	Trypanosomatidae	28%	97%
A0A3Q8IBB4	Leishmania donovani	94%	100%
A0A3Q8IIK0	Leishmania donovani	28%	95%
A0A3R7NTC0	Trypanosoma rangeli	29%	98%
A0A3S5IRW3	Trypanosoma rangeli	29%	87%
A0A3S7X3U9	Leishmania donovani	27%	97%
A4HCJ2	Leishmania braziliensis	79%	100%
A4HH45	Leishmania braziliensis	28%	95%
A4HIR7	Leishmania braziliensis	28%	86%
A4I008	Leishmania infantum	94%	100%
A4I498	Leishmania infantum	28%	95%
A4I612	Leishmania infantum	27%	97%
C9ZQ86	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	48%	97%
C9ZRD7	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	27%	81%
E9ADP5	Leishmania major	29%	100%
E9AM35	Leishmania mexicana (strain MHOM/GT/2001/U1103)	29%	95%
E9AVY1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	91%	100%
E9B1A3	Leishmania mexicana (strain MHOM/GT/2001/U1103)	27%	97%
O74480	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	30%	100%
Q4Q6W1	Leishmania major	27%	100%
Q8L774	Arabidopsis thaliana	25%	93%
Q944L7	Arabidopsis thaliana	26%	92%
V5ARZ9	Trypanosoma cruzi	46%	96%
V5BPY7	Trypanosoma cruzi	32%	82%
V5BX32	Trypanosoma cruzi	29%	98%