Checkpoint protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Checkpoint protein

Gene product:

Checkpoint protein HUS1

Species:

Leishmania major

UniProt:

Q4QBE5_LEIMA

TriTrypDb:

LmjF.23.0290 , LMJLV39_230008200 , LMJSD75_230008400

Length:

331

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 8
NetGPI	no	yes: 0, no: 8

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	2
GO:0005730	nucleolus	5	9
GO:0016020	membrane	2	5
GO:0030896	checkpoint clamp complex	3	9
GO:0032991	protein-containing complex	1	9
GO:0035861	site of double-strand break	3	2
GO:0043226	organelle	2	9
GO:0043227	membrane-bounded organelle	3	2
GO:0043228	non-membrane-bounded organelle	3	9
GO:0043229	intracellular organelle	3	9
GO:0043231	intracellular membrane-bounded organelle	4	2
GO:0043232	intracellular non-membrane-bounded organelle	4	9
GO:0090734	site of DNA damage	2	2
GO:0110165	cellular anatomical entity	1	9
GO:0140513	nuclear protein-containing complex	2	9

Expansion

Sequence features

Q4QBE5

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4QBE5

Function

Biological processes
Term	Name	Level	Count
GO:0000075	cell cycle checkpoint signaling	4	9
GO:0000076	DNA replication checkpoint signaling	6	2
GO:0000077	DNA damage checkpoint signaling	5	9
GO:0000723	telomere maintenance	5	2
GO:0000724	double-strand break repair via homologous recombination	7	2
GO:0000725	recombinational repair	6	2
GO:0006139	nucleobase-containing compound metabolic process	3	2
GO:0006259	DNA metabolic process	4	2
GO:0006281	DNA repair	5	2
GO:0006289	nucleotide-excision repair	6	2
GO:0006302	double-strand break repair	6	2
GO:0006310	DNA recombination	5	2
GO:0006725	cellular aromatic compound metabolic process	3	2
GO:0006807	nitrogen compound metabolic process	2	2
GO:0006950	response to stress	2	9
GO:0006974	DNA damage response	4	9
GO:0006996	organelle organization	4	2
GO:0007093	mitotic cell cycle checkpoint signaling	4	2
GO:0007165	signal transduction	2	9
GO:0007346	regulation of mitotic cell cycle	5	2
GO:0008152	metabolic process	1	2
GO:0009987	cellular process	1	9
GO:0010389	regulation of G2/M transition of mitotic cell cycle	7	2
GO:0010564	regulation of cell cycle process	5	9
GO:0010948	negative regulation of cell cycle process	6	9
GO:0010972	negative regulation of G2/M transition of mitotic cell cycle	8	2
GO:0016043	cellular component organization	3	2
GO:0022402	cell cycle process	2	2
GO:0022414	reproductive process	1	2
GO:0031570	DNA integrity checkpoint signaling	5	9
GO:0031573	mitotic intra-S DNA damage checkpoint signaling	7	2
GO:0032200	telomere organization	6	2
GO:0033313	meiotic cell cycle checkpoint signaling	3	2
GO:0033314	mitotic DNA replication checkpoint signaling	6	2
GO:0033554	cellular response to stress	3	9
GO:0034641	cellular nitrogen compound metabolic process	3	2
GO:0035556	intracellular signal transduction	3	9
GO:0042770	signal transduction in response to DNA damage	4	9
GO:0043170	macromolecule metabolic process	3	2
GO:0044237	cellular metabolic process	2	2
GO:0044238	primary metabolic process	2	2
GO:0044260	obsolete cellular macromolecule metabolic process	3	2
GO:0044773	mitotic DNA damage checkpoint signaling	6	2
GO:0044774	mitotic DNA integrity checkpoint signaling	5	2
GO:0044778	meiotic DNA integrity checkpoint signaling	4	2
GO:0044818	mitotic G2/M transition checkpoint	5	2
GO:0045786	negative regulation of cell cycle	5	9
GO:0045930	negative regulation of mitotic cell cycle	6	2
GO:0046483	heterocycle metabolic process	3	2
GO:0048519	negative regulation of biological process	3	9
GO:0048523	negative regulation of cellular process	4	9
GO:0050789	regulation of biological process	2	9
GO:0050794	regulation of cellular process	3	9
GO:0050896	response to stimulus	1	9
GO:0051276	chromosome organization	5	2
GO:0051716	cellular response to stimulus	2	9
GO:0051726	regulation of cell cycle	4	9
GO:0065007	biological regulation	1	9
GO:0071704	organic substance metabolic process	2	2
GO:0071840	cellular component organization or biogenesis	2	2
GO:0090304	nucleic acid metabolic process	4	2
GO:1901360	organic cyclic compound metabolic process	3	2
GO:1901987	regulation of cell cycle phase transition	6	9
GO:1901988	negative regulation of cell cycle phase transition	7	9
GO:1901990	regulation of mitotic cell cycle phase transition	6	2
GO:1901991	negative regulation of mitotic cell cycle phase transition	7	2
GO:1902749	regulation of cell cycle G2/M phase transition	7	2
GO:1902750	negative regulation of cell cycle G2/M phase transition	8	2
GO:1903046	meiotic cell cycle process	2	2
GO:1903047	mitotic cell cycle process	3	2

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	2
GO:0003677	DNA binding	4	2
GO:0003684	damaged DNA binding	5	2
GO:0005488	binding	1	2
GO:0097159	organic cyclic compound binding	2	2
GO:1901363	heterocyclic compound binding	2	2

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	178	182	PF00656	0.560
CLV_C14_Caspase3-7	205	209	PF00656	0.515
CLV_C14_Caspase3-7	6	10	PF00656	0.598
CLV_NRD_NRD_1	196	198	PF00675	0.315
CLV_NRD_NRD_1	214	216	PF00675	0.348
CLV_NRD_NRD_1	283	285	PF00675	0.360
CLV_NRD_NRD_1	308	310	PF00675	0.339
CLV_PCSK_KEX2_1	196	198	PF00082	0.315
CLV_PCSK_KEX2_1	214	216	PF00082	0.358
CLV_PCSK_KEX2_1	218	220	PF00082	0.362
CLV_PCSK_KEX2_1	308	310	PF00082	0.339
CLV_PCSK_PC1ET2_1	218	220	PF00082	0.360
CLV_PCSK_PC7_1	214	220	PF00082	0.348
CLV_PCSK_SKI1_1	215	219	PF00082	0.365
CLV_PCSK_SKI1_1	223	227	PF00082	0.379
CLV_PCSK_SKI1_1	275	279	PF00082	0.342
CLV_PCSK_SKI1_1	308	312	PF00082	0.313
CLV_PCSK_SKI1_1	8	12	PF00082	0.364
DEG_APCC_DBOX_1	113	121	PF00400	0.598
DEG_APCC_DBOX_1	154	162	PF00400	0.515
DEG_Nend_UBRbox_1	1	4	PF02207	0.501
DOC_CKS1_1	192	197	PF01111	0.567
DOC_CYCLIN_yCln2_LP_2	190	193	PF00134	0.542
DOC_MAPK_gen_1	218	228	PF00069	0.598
DOC_MAPK_gen_1	308	315	PF00069	0.539
DOC_MAPK_gen_1	8	16	PF00069	0.594
DOC_MAPK_MEF2A_6	155	162	PF00069	0.560
DOC_MAPK_MEF2A_6	308	315	PF00069	0.519
DOC_MAPK_NFAT4_5	308	316	PF00069	0.539
DOC_PP1_RVXF_1	33	39	PF00149	0.560
DOC_PP2B_LxvP_1	148	151	PF13499	0.598
DOC_PP2B_LxvP_1	190	193	PF13499	0.555
DOC_PP4_FxxP_1	133	136	PF00568	0.536
DOC_USP7_MATH_1	129	133	PF00917	0.570
DOC_USP7_MATH_1	179	183	PF00917	0.591
DOC_USP7_MATH_1	227	231	PF00917	0.503
DOC_USP7_UBL2_3	4	8	PF12436	0.554
DOC_WW_Pin1_4	146	151	PF00397	0.598
DOC_WW_Pin1_4	191	196	PF00397	0.567
LIG_14-3-3_CanoR_1	2	8	PF00244	0.598
LIG_14-3-3_CanoR_1	241	245	PF00244	0.560
LIG_14-3-3_CanoR_1	275	280	PF00244	0.583
LIG_14-3-3_CanoR_1	288	296	PF00244	0.468
LIG_14-3-3_CanoR_1	30	39	PF00244	0.572
LIG_14-3-3_CanoR_1	53	59	PF00244	0.599
LIG_Actin_WH2_2	161	178	PF00022	0.560
LIG_APCC_ABBA_1	231	236	PF00400	0.560
LIG_BIR_III_2	224	228	PF00653	0.431
LIG_BIR_III_4	206	210	PF00653	0.515
LIG_BIR_III_4	9	13	PF00653	0.560
LIG_BRCT_BRCA1_1	181	185	PF00533	0.598
LIG_BRCT_BRCA1_1	56	60	PF00533	0.558
LIG_eIF4E_1	246	252	PF01652	0.560
LIG_FHA_1	100	106	PF00498	0.558
LIG_FHA_1	167	173	PF00498	0.560
LIG_FHA_1	276	282	PF00498	0.525
LIG_FHA_1	319	325	PF00498	0.358
LIG_FHA_1	73	79	PF00498	0.560
LIG_FHA_2	168	174	PF00498	0.520
LIG_FHA_2	176	182	PF00498	0.478
LIG_FHA_2	4	10	PF00498	0.525
LIG_Integrin_isoDGR_2	112	114	PF01839	0.398
LIG_LIR_Apic_2	130	136	PF02991	0.509
LIG_LIR_Gen_1	243	252	PF02991	0.560
LIG_LIR_Gen_1	283	293	PF02991	0.560
LIG_LIR_Nem_3	243	249	PF02991	0.525
LIG_LIR_Nem_3	283	289	PF02991	0.560
LIG_LIR_Nem_3	56	61	PF02991	0.454
LIG_PCNA_yPIPBox_3	320	330	PF02747	0.442
LIG_Pex14_2	238	242	PF04695	0.523
LIG_Pex14_2	286	290	PF04695	0.560
LIG_SH2_NCK_1	246	250	PF00017	0.560
LIG_SH2_SRC	234	237	PF00017	0.530
LIG_SH2_SRC	326	329	PF00017	0.438
LIG_SH2_SRC	69	72	PF00017	0.598
LIG_SH2_STAP1	234	238	PF00017	0.563
LIG_SH2_STAT5	304	307	PF00017	0.560
LIG_SH2_STAT5	326	329	PF00017	0.438
LIG_SH3_2	192	197	PF14604	0.515
LIG_SH3_3	186	192	PF00018	0.560
LIG_SH3_3	214	220	PF00018	0.598
LIG_SUMO_SIM_anti_2	247	253	PF11976	0.602
LIG_SUMO_SIM_par_1	164	170	PF11976	0.560
LIG_SUMO_SIM_par_1	277	283	PF11976	0.526
LIG_TYR_ITIM	244	249	PF00017	0.523
LIG_TYR_ITIM	59	64	PF00017	0.431
LIG_UBA3_1	84	89	PF00899	0.560
LIG_WRC_WIRS_1	168	173	PF05994	0.560
MOD_CDK_SPK_2	191	196	PF00069	0.587
MOD_CDK_SPxK_1	191	197	PF00069	0.515
MOD_GlcNHglycan	125	128	PF01048	0.392
MOD_GlcNHglycan	185	188	PF01048	0.339
MOD_GlcNHglycan	290	293	PF01048	0.398
MOD_GlcNHglycan	66	69	PF01048	0.398
MOD_GSK3_1	175	182	PF00069	0.583
MOD_GSK3_1	94	101	PF00069	0.523
MOD_NEK2_1	166	171	PF00069	0.548
MOD_NEK2_1	175	180	PF00069	0.498
MOD_NEK2_1	280	285	PF00069	0.573
MOD_NEK2_1	299	304	PF00069	0.478
MOD_NEK2_1	3	8	PF00069	0.525
MOD_NEK2_1	311	316	PF00069	0.295
MOD_NEK2_1	39	44	PF00069	0.562
MOD_NEK2_2	240	245	PF00069	0.552
MOD_PKA_2	240	246	PF00069	0.598
MOD_PKB_1	35	43	PF00069	0.525
MOD_Plk_1	129	135	PF00069	0.560
MOD_Plk_1	166	172	PF00069	0.525
MOD_Plk_1	70	76	PF00069	0.523
MOD_Plk_2-3	167	173	PF00069	0.560
MOD_Plk_4	167	173	PF00069	0.527
MOD_Plk_4	227	233	PF00069	0.510
MOD_Plk_4	275	281	PF00069	0.598
MOD_Plk_4	299	305	PF00069	0.582
MOD_Plk_4	311	317	PF00069	0.341
MOD_Plk_4	54	60	PF00069	0.503
MOD_ProDKin_1	146	152	PF00069	0.598
MOD_ProDKin_1	191	197	PF00069	0.567
MOD_SUMO_for_1	244	247	PF00179	0.560
TRG_DiLeu_BaEn_1	247	252	PF01217	0.560
TRG_DiLeu_BaEn_2	166	172	PF01217	0.501
TRG_ENDOCYTIC_2	246	249	PF00928	0.501
TRG_ENDOCYTIC_2	61	64	PF00928	0.601
TRG_ER_diArg_1	195	197	PF00400	0.515
TRG_ER_diArg_1	213	215	PF00400	0.558
TRG_ER_diArg_1	35	38	PF00400	0.501
TRG_NES_CRM1_1	295	307	PF08389	0.560
TRG_Pf-PMV_PEXEL_1	138	142	PF00026	0.301

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I495	Leptomonas seymouri	63%	99%
A0A1X0NG57	Trypanosomatidae	35%	100%
A0A3S5H7B6	Leishmania donovani	95%	100%
A0A422NW79	Trypanosoma rangeli	40%	100%
A4HCP3	Leishmania braziliensis	84%	100%
A4I069	Leishmania infantum	95%	100%
E9AW32	Leishmania mexicana (strain MHOM/GT/2001/U1103)	95%	100%