Tryptophan--tRNA ligase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

tryptophanyl-tRNA synthetase, putative

Species:

Leishmania major

UniProt:

Q4QBE4_LEIMA

TriTrypDb:

LmjF.23.0300 , LMJLV39_230008300 , LMJSD75_230008500

Length:

412

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	2
GO:0110165	cellular anatomical entity	1	2

Expansion

Sequence features

Q4QBE4

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4QBE4

Function

Biological processes
Term	Name	Level	Count
GO:0006082	organic acid metabolic process	3	11
GO:0006139	nucleobase-containing compound metabolic process	3	11
GO:0006399	tRNA metabolic process	7	11
GO:0006418	tRNA aminoacylation for protein translation	6	11
GO:0006436	tryptophanyl-tRNA aminoacylation	7	11
GO:0006520	amino acid metabolic process	3	11
GO:0006725	cellular aromatic compound metabolic process	3	11
GO:0006807	nitrogen compound metabolic process	2	11
GO:0008152	metabolic process	1	11
GO:0009987	cellular process	1	11
GO:0016070	RNA metabolic process	5	11
GO:0019752	carboxylic acid metabolic process	5	11
GO:0034641	cellular nitrogen compound metabolic process	3	11
GO:0034660	ncRNA metabolic process	6	11
GO:0043038	amino acid activation	4	11
GO:0043039	tRNA aminoacylation	5	11
GO:0043170	macromolecule metabolic process	3	11
GO:0043436	oxoacid metabolic process	4	11
GO:0044237	cellular metabolic process	2	11
GO:0044238	primary metabolic process	2	11
GO:0044281	small molecule metabolic process	2	11
GO:0046483	heterocycle metabolic process	3	11
GO:0071704	organic substance metabolic process	2	11
GO:0090304	nucleic acid metabolic process	4	11
GO:1901360	organic cyclic compound metabolic process	3	11
GO:1901564	organonitrogen compound metabolic process	3	11

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	11
GO:0003824	catalytic activity	1	11
GO:0004812	aminoacyl-tRNA ligase activity	4	11
GO:0004830	tryptophan-tRNA ligase activity	5	11
GO:0005488	binding	1	11
GO:0005524	ATP binding	5	11
GO:0016874	ligase activity	2	11
GO:0016875	ligase activity, forming carbon-oxygen bonds	3	11
GO:0017076	purine nucleotide binding	4	11
GO:0030554	adenyl nucleotide binding	5	11
GO:0032553	ribonucleotide binding	3	11
GO:0032555	purine ribonucleotide binding	4	11
GO:0032559	adenyl ribonucleotide binding	5	11
GO:0035639	purine ribonucleoside triphosphate binding	4	11
GO:0036094	small molecule binding	2	11
GO:0043167	ion binding	2	11
GO:0043168	anion binding	3	11
GO:0097159	organic cyclic compound binding	2	11
GO:0097367	carbohydrate derivative binding	2	11
GO:0140098	catalytic activity, acting on RNA	3	11
GO:0140101	catalytic activity, acting on a tRNA	4	11
GO:0140640	catalytic activity, acting on a nucleic acid	2	11
GO:1901265	nucleoside phosphate binding	3	11
GO:1901363	heterocyclic compound binding	2	11

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	212	216	PF00656	0.456
CLV_NRD_NRD_1	16	18	PF00675	0.534
CLV_NRD_NRD_1	236	238	PF00675	0.272
CLV_NRD_NRD_1	312	314	PF00675	0.260
CLV_NRD_NRD_1	317	319	PF00675	0.244
CLV_NRD_NRD_1	392	394	PF00675	0.301
CLV_PCSK_FUR_1	390	394	PF00082	0.439
CLV_PCSK_KEX2_1	18	20	PF00082	0.562
CLV_PCSK_KEX2_1	236	238	PF00082	0.272
CLV_PCSK_KEX2_1	317	319	PF00082	0.270
CLV_PCSK_KEX2_1	392	394	PF00082	0.307
CLV_PCSK_PC1ET2_1	18	20	PF00082	0.456
CLV_PCSK_PC7_1	313	319	PF00082	0.256
CLV_PCSK_SKI1_1	143	147	PF00082	0.223
CLV_PCSK_SKI1_1	19	23	PF00082	0.467
CLV_PCSK_SKI1_1	198	202	PF00082	0.209
CLV_PCSK_SKI1_1	219	223	PF00082	0.237
CLV_PCSK_SKI1_1	236	240	PF00082	0.176
CLV_PCSK_SKI1_1	317	321	PF00082	0.248
CLV_PCSK_SKI1_1	383	387	PF00082	0.384
CLV_PCSK_SKI1_1	392	396	PF00082	0.353
CLV_PCSK_SKI1_1	408	412	PF00082	0.305
CLV_PCSK_SKI1_1	97	101	PF00082	0.185
DEG_Nend_UBRbox_2	1	3	PF02207	0.399
DOC_CKS1_1	32	37	PF01111	0.592
DOC_MAPK_DCC_7	58	66	PF00069	0.480
DOC_MAPK_MEF2A_6	58	66	PF00069	0.480
DOC_PP1_RVXF_1	217	223	PF00149	0.488
DOC_PP1_RVXF_1	70	77	PF00149	0.312
DOC_PP4_FxxP_1	130	133	PF00568	0.465
DOC_PP4_FxxP_1	348	351	PF00568	0.446
DOC_USP7_UBL2_3	310	314	PF12436	0.501
DOC_WW_Pin1_4	21	26	PF00397	0.629
DOC_WW_Pin1_4	31	36	PF00397	0.679
DOC_WW_Pin1_4	49	54	PF00397	0.446
LIG_14-3-3_CanoR_1	17	22	PF00244	0.585
LIG_14-3-3_CanoR_1	175	179	PF00244	0.427
LIG_14-3-3_CanoR_1	181	189	PF00244	0.414
LIG_14-3-3_CanoR_1	195	201	PF00244	0.322
LIG_14-3-3_CanoR_1	392	397	PF00244	0.342
LIG_AP2alpha_2	257	259	PF02296	0.423
LIG_BIR_III_2	38	42	PF00653	0.512
LIG_BRCT_BRCA1_1	231	235	PF00533	0.423
LIG_Clathr_ClatBox_1	85	89	PF01394	0.329
LIG_FHA_1	109	115	PF00498	0.444
LIG_FHA_1	121	127	PF00498	0.364
LIG_FHA_1	206	212	PF00498	0.443
LIG_FHA_1	278	284	PF00498	0.518
LIG_FHA_1	32	38	PF00498	0.561
LIG_FHA_2	137	143	PF00498	0.423
LIG_FHA_2	210	216	PF00498	0.456
LIG_FHA_2	302	308	PF00498	0.424
LIG_FHA_2	393	399	PF00498	0.319
LIG_LIR_Apic_2	128	133	PF02991	0.465
LIG_LIR_Gen_1	340	351	PF02991	0.423
LIG_LIR_Gen_1	353	363	PF02991	0.423
LIG_LIR_Gen_1	403	412	PF02991	0.356
LIG_LIR_Nem_3	188	193	PF02991	0.408
LIG_LIR_Nem_3	232	238	PF02991	0.410
LIG_LIR_Nem_3	257	262	PF02991	0.409
LIG_LIR_Nem_3	340	346	PF02991	0.436
LIG_LIR_Nem_3	353	359	PF02991	0.491
LIG_LIR_Nem_3	403	407	PF02991	0.317
LIG_LYPXL_S_1	189	193	PF13949	0.265
LIG_LYPXL_yS_3	190	193	PF13949	0.465
LIG_MAD2	237	245	PF02301	0.504
LIG_PDZ_Class_2	407	412	PF00595	0.320
LIG_Pex14_2	231	235	PF04695	0.409
LIG_PTB_Apo_2	179	186	PF02174	0.430
LIG_PTB_Phospho_1	179	185	PF10480	0.430
LIG_REV1ctd_RIR_1	67	76	PF16727	0.318
LIG_SH2_CRK	343	347	PF00017	0.423
LIG_SH2_STAT5	102	105	PF00017	0.414
LIG_SH2_STAT5	125	128	PF00017	0.439
LIG_SH2_STAT5	345	348	PF00017	0.506
LIG_SH3_3	29	35	PF00018	0.553
LIG_SUMO_SIM_par_1	301	309	PF11976	0.423
LIG_SUMO_SIM_par_1	84	89	PF11976	0.422
LIG_TRAF2_1	296	299	PF00917	0.547
LIG_TYR_ITIM	341	346	PF00017	0.423
MOD_CK1_1	108	114	PF00069	0.379
MOD_CK1_1	91	97	PF00069	0.386
MOD_CK2_1	136	142	PF00069	0.423
MOD_CK2_1	292	298	PF00069	0.417
MOD_GlcNHglycan	107	110	PF01048	0.198
MOD_GlcNHglycan	25	28	PF01048	0.648
MOD_GlcNHglycan	295	298	PF01048	0.215
MOD_GlcNHglycan	320	323	PF01048	0.209
MOD_GlcNHglycan	364	367	PF01048	0.267
MOD_GlcNHglycan	369	372	PF01048	0.275
MOD_GSK3_1	17	24	PF00069	0.516
MOD_GSK3_1	205	212	PF00069	0.542
MOD_GSK3_1	301	308	PF00069	0.437
MOD_GSK3_1	91	98	PF00069	0.359
MOD_NEK2_1	100	105	PF00069	0.483
MOD_NEK2_1	120	125	PF00069	0.322
MOD_NEK2_1	159	164	PF00069	0.420
MOD_NEK2_1	180	185	PF00069	0.415
MOD_NEK2_1	196	201	PF00069	0.390
MOD_NEK2_1	260	265	PF00069	0.423
MOD_NEK2_1	277	282	PF00069	0.406
MOD_NEK2_1	330	335	PF00069	0.544
MOD_NEK2_2	301	306	PF00069	0.440
MOD_NEK2_2	351	356	PF00069	0.420
MOD_NEK2_2	95	100	PF00069	0.259
MOD_PIKK_1	222	228	PF00454	0.465
MOD_PIKK_1	357	363	PF00454	0.456
MOD_PKA_1	17	23	PF00069	0.419
MOD_PKA_1	392	398	PF00069	0.441
MOD_PKA_2	174	180	PF00069	0.427
MOD_PKA_2	392	398	PF00069	0.443
MOD_PKB_1	17	25	PF00069	0.474
MOD_PKB_1	362	370	PF00069	0.385
MOD_PKB_1	390	398	PF00069	0.342
MOD_Plk_1	351	357	PF00069	0.431
MOD_Plk_1	88	94	PF00069	0.393
MOD_Plk_1	95	101	PF00069	0.244
MOD_Plk_4	174	180	PF00069	0.419
MOD_Plk_4	189	195	PF00069	0.385
MOD_Plk_4	351	357	PF00069	0.510
MOD_ProDKin_1	21	27	PF00069	0.633
MOD_ProDKin_1	31	37	PF00069	0.678
MOD_ProDKin_1	49	55	PF00069	0.444
MOD_SUMO_rev_2	140	145	PF00179	0.409
MOD_SUMO_rev_2	294	302	PF00179	0.435
MOD_SUMO_rev_2	370	376	PF00179	0.502
TRG_DiLeu_BaEn_2	95	101	PF01217	0.297
TRG_ENDOCYTIC_2	190	193	PF00928	0.418
TRG_ENDOCYTIC_2	343	346	PF00928	0.423
TRG_ER_diArg_1	235	237	PF00400	0.472
TRG_ER_diArg_1	316	318	PF00400	0.465
TRG_NLS_MonoExtC_3	312	317	PF00514	0.425
TRG_NLS_MonoExtN_4	15	21	PF00514	0.442
TRG_NLS_MonoExtN_4	310	317	PF00514	0.504

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P3G3	Leptomonas seymouri	43%	100%
A0A0N1HXL2	Leptomonas seymouri	71%	88%
A0A0S4IN16	Bodo saltans	46%	100%
A0A0S4JC70	Bodo saltans	54%	91%
A0A1X0NGJ6	Trypanosomatidae	62%	89%
A0A1X0NZB4	Trypanosomatidae	46%	100%
A0A3R7MPN7	Trypanosoma rangeli	46%	100%
A0A3S7WXL2	Leishmania donovani	94%	100%
A0A3S7X238	Leishmania donovani	42%	100%
A0A422NW64	Trypanosoma rangeli	62%	92%
A2BLD4	Hyperthermus butylicus (strain DSM 5456 / JCM 9403 / PLM1-5)	28%	100%
A3MX72	Pyrobaculum calidifontis (strain DSM 21063 / JCM 11548 / VA1)	36%	100%
A4HCP4	Leishmania braziliensis	82%	84%
A4HH14	Leishmania braziliensis	43%	87%
A4I070	Leishmania infantum	94%	100%
A4I443	Leishmania infantum	42%	100%
A4WL99	Pyrobaculum arsenaticum (strain DSM 13514 / JCM 11321 / PZ6)	37%	100%
B6YUH1	Thermococcus onnurineus (strain NA1)	40%	100%
C6A032	Thermococcus sibiricus (strain DSM 12597 / MM 739)	37%	100%
C9ZKR9	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	48%	100%
E9ADK8	Leishmania major	44%	100%
E9AM73	Leishmania mexicana (strain MHOM/GT/2001/U1103)	41%	100%
E9AW33	Leishmania mexicana (strain MHOM/GT/2001/U1103)	94%	100%
O26352	Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H)	28%	100%
O59584	Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)	40%	100%
O96771	Encephalitozoon cuniculi (strain GB-M1)	39%	100%
P17248	Bos taurus	45%	87%
P23381	Homo sapiens	45%	87%
P23612	Oryctolagus cuniculus	44%	87%
P32921	Mus musculus	45%	86%
Q09692	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	46%	100%
Q12109	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	45%	95%
Q4JBG7	Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)	42%	100%
Q55DZ8	Dictyostelium discoideum	44%	100%
Q58810	Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)	31%	100%
Q5JEP3	Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1)	40%	100%
Q5R4J1	Pongo abelii	44%	87%
Q6P7B0	Rattus norvegicus	45%	86%
Q8TYF7	Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938)	27%	100%
Q8U453	Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)	40%	100%
Q8ZTU5	Pyrobaculum aerophilum (strain ATCC 51768 / DSM 7523 / JCM 9630 / CIP 104966 / NBRC 100827 / IM2)	38%	100%
Q976M1	Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7)	41%	100%
Q97ZX0	Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)	41%	100%
Q9HN66	Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1)	38%	100%
Q9SR15	Arabidopsis thaliana	41%	100%
Q9UY11	Pyrococcus abyssi (strain GE5 / Orsay)	40%	100%
Q9Y924	Aeropyrum pernix (strain ATCC 700893 / DSM 11879 / JCM 9820 / NBRC 100138 / K1)	31%	100%
V5B4S5	Trypanosoma cruzi	45%	100%