Putative mitochondrial DNA primase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

mitochondrial DNA primase, putative

Species:

Leishmania major

UniProt:

Q4QB95_LEIMA

TriTrypDb:

LmjF.23.0680 , LMJLV39_230015300 , LMJSD75_230015600

Length:

532

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005654	nucleoplasm	2	2
GO:0005730	nucleolus	5	2
GO:0005759	mitochondrial matrix	5	2
GO:0020023	kinetoplast	2	2
GO:0031974	membrane-enclosed lumen	2	2
GO:0043226	organelle	2	2
GO:0043228	non-membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043232	intracellular non-membrane-bounded organelle	4	2
GO:0043233	organelle lumen	3	2
GO:0070013	intracellular organelle lumen	4	2
GO:0110165	cellular anatomical entity	1	2

Expansion

Sequence features

Q4QB95

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4QB95

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	2
GO:0006259	DNA metabolic process	4	2
GO:0006260	DNA replication	5	2
GO:0006261	DNA-templated DNA replication	6	2
GO:0006264	mitochondrial DNA replication	6	2
GO:0006725	cellular aromatic compound metabolic process	3	2
GO:0006807	nitrogen compound metabolic process	2	2
GO:0008152	metabolic process	1	2
GO:0009987	cellular process	1	2
GO:0032042	mitochondrial DNA metabolic process	5	2
GO:0034641	cellular nitrogen compound metabolic process	3	2
GO:0043170	macromolecule metabolic process	3	2
GO:0044237	cellular metabolic process	2	2
GO:0044238	primary metabolic process	2	2
GO:0044260	obsolete cellular macromolecule metabolic process	3	2
GO:0046483	heterocycle metabolic process	3	2
GO:0071704	organic substance metabolic process	2	2
GO:0090304	nucleic acid metabolic process	4	2
GO:1901360	organic cyclic compound metabolic process	3	2

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	11
GO:0003896	DNA primase activity	7	2
GO:0003899	DNA-directed 5'-3' RNA polymerase activity	6	5
GO:0016740	transferase activity	2	5
GO:0016772	transferase activity, transferring phosphorus-containing groups	3	5
GO:0016779	nucleotidyltransferase activity	4	5
GO:0016787	hydrolase activity	2	11
GO:0016817	hydrolase activity, acting on acid anhydrides	3	11
GO:0034062	5'-3' RNA polymerase activity	5	5
GO:0097747	RNA polymerase activity	4	5
GO:0140098	catalytic activity, acting on RNA	3	5
GO:0140640	catalytic activity, acting on a nucleic acid	2	5

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	119	123	PF00656	0.349
CLV_C14_Caspase3-7	420	424	PF00656	0.295
CLV_NRD_NRD_1	100	102	PF00675	0.451
CLV_NRD_NRD_1	127	129	PF00675	0.317
CLV_NRD_NRD_1	196	198	PF00675	0.420
CLV_NRD_NRD_1	429	431	PF00675	0.393
CLV_NRD_NRD_1	5	7	PF00675	0.668
CLV_NRD_NRD_1	519	521	PF00675	0.669
CLV_NRD_NRD_1	530	532	PF00675	0.690
CLV_PCSK_KEX2_1	127	129	PF00082	0.336
CLV_PCSK_KEX2_1	464	466	PF00082	0.498
CLV_PCSK_KEX2_1	530	532	PF00082	0.704
CLV_PCSK_PC1ET2_1	464	466	PF00082	0.439
CLV_PCSK_SKI1_1	197	201	PF00082	0.290
CLV_PCSK_SKI1_1	327	331	PF00082	0.483
CLV_PCSK_SKI1_1	380	384	PF00082	0.349
CLV_PCSK_SKI1_1	520	524	PF00082	0.673
CLV_PCSK_SKI1_1	526	530	PF00082	0.687
DEG_APCC_DBOX_1	196	204	PF00400	0.409
DEG_APCC_KENBOX_2	282	286	PF00400	0.392
DEG_Nend_Nbox_1	1	3	PF02207	0.607
DEG_SCF_FBW7_2	203	209	PF00400	0.324
DOC_ANK_TNKS_1	5	12	PF00023	0.737
DOC_CKS1_1	140	145	PF01111	0.295
DOC_CKS1_1	203	208	PF01111	0.324
DOC_CYCLIN_RxL_1	324	332	PF00134	0.389
DOC_CYCLIN_yCln2_LP_2	413	419	PF00134	0.324
DOC_MAPK_DCC_7	257	267	PF00069	0.372
DOC_MAPK_gen_1	283	293	PF00069	0.558
DOC_MAPK_gen_1	377	387	PF00069	0.405
DOC_MAPK_gen_1	430	436	PF00069	0.411
DOC_MAPK_gen_1	464	471	PF00069	0.453
DOC_MAPK_HePTP_8	164	176	PF00069	0.295
DOC_MAPK_MEF2A_6	167	176	PF00069	0.295
DOC_MAPK_MEF2A_6	261	269	PF00069	0.371
DOC_MAPK_MEF2A_6	358	366	PF00069	0.440
DOC_MAPK_MEF2A_6	391	398	PF00069	0.277
DOC_MAPK_MEF2A_6	464	473	PF00069	0.467
DOC_MAPK_NFAT4_5	391	399	PF00069	0.295
DOC_PP1_RVXF_1	325	332	PF00149	0.391
DOC_PP2B_LxvP_1	357	360	PF13499	0.637
DOC_PP2B_LxvP_1	469	472	PF13499	0.481
DOC_PP4_FxxP_1	137	140	PF00568	0.277
DOC_PP4_FxxP_1	331	334	PF00568	0.398
DOC_USP7_MATH_1	116	120	PF00917	0.423
DOC_USP7_MATH_1	150	154	PF00917	0.371
DOC_USP7_MATH_1	313	317	PF00917	0.370
DOC_USP7_MATH_1	334	338	PF00917	0.513
DOC_USP7_MATH_1	371	375	PF00917	0.433
DOC_USP7_MATH_1	511	515	PF00917	0.609
DOC_USP7_MATH_1	9	13	PF00917	0.741
DOC_USP7_UBL2_3	431	435	PF12436	0.277
DOC_USP7_UBL2_3	523	527	PF12436	0.705
DOC_WW_Pin1_4	139	144	PF00397	0.303
DOC_WW_Pin1_4	167	172	PF00397	0.316
DOC_WW_Pin1_4	202	207	PF00397	0.324
DOC_WW_Pin1_4	223	228	PF00397	0.277
LIG_14-3-3_CanoR_1	101	109	PF00244	0.442
LIG_14-3-3_CanoR_1	286	293	PF00244	0.453
LIG_14-3-3_CanoR_1	327	332	PF00244	0.510
LIG_14-3-3_CanoR_1	380	387	PF00244	0.449
LIG_Actin_WH2_2	215	232	PF00022	0.295
LIG_APCC_ABBA_1	290	295	PF00400	0.340
LIG_BIR_III_4	81	85	PF00653	0.247
LIG_deltaCOP1_diTrp_1	294	302	PF00928	0.371
LIG_deltaCOP1_diTrp_1	389	393	PF00928	0.296
LIG_deltaCOP1_diTrp_1	442	448	PF00928	0.277
LIG_FHA_1	101	107	PF00498	0.295
LIG_FHA_1	218	224	PF00498	0.419
LIG_FHA_1	285	291	PF00498	0.393
LIG_FHA_2	168	174	PF00498	0.390
LIG_FHA_2	28	34	PF00498	0.616
LIG_FHA_2	365	371	PF00498	0.388
LIG_FHA_2	381	387	PF00498	0.236
LIG_HCF-1_HBM_1	54	57	PF13415	0.545
LIG_LIR_Apic_2	330	334	PF02991	0.523
LIG_LIR_Gen_1	242	251	PF02991	0.313
LIG_LIR_Gen_1	323	333	PF02991	0.418
LIG_LIR_Gen_1	442	453	PF02991	0.277
LIG_LIR_Nem_3	242	248	PF02991	0.302
LIG_LIR_Nem_3	323	328	PF02991	0.414
LIG_LIR_Nem_3	392	396	PF02991	0.310
LIG_LIR_Nem_3	442	448	PF02991	0.317
LIG_LIR_Nem_3	457	463	PF02991	0.278
LIG_MAD2	198	206	PF02301	0.349
LIG_OCRL_FandH_1	247	259	PF00620	0.432
LIG_Pex14_1	298	302	PF04695	0.355
LIG_Pex14_1	459	463	PF04695	0.277
LIG_Pex14_2	254	258	PF04695	0.337
LIG_SH2_CRK	463	467	PF00017	0.378
LIG_SH2_NCK_1	71	75	PF00017	0.212
LIG_SH3_3	137	143	PF00018	0.295
LIG_SH3_3	149	155	PF00018	0.295
LIG_SH3_3	200	206	PF00018	0.349
LIG_SH3_3	316	322	PF00018	0.435
LIG_SH3_3	359	365	PF00018	0.492
LIG_SH3_3	497	503	PF00018	0.599
LIG_SUMO_SIM_anti_2	170	177	PF11976	0.320
LIG_SUMO_SIM_par_1	142	149	PF11976	0.269
LIG_TRAF2_1	12	15	PF00917	0.821
LIG_TRAF2_1	30	33	PF00917	0.621
LIG_TRAF2_1	45	48	PF00917	0.599
LIG_UBA3_1	1	7	PF00899	0.738
LIG_UBA3_1	275	283	PF00899	0.333
LIG_UBA3_1	363	369	PF00899	0.376
LIG_WRC_WIRS_1	328	333	PF05994	0.450
MOD_CDK_SPxK_1	202	208	PF00069	0.324
MOD_CDK_SPxxK_3	223	230	PF00069	0.277
MOD_CK1_1	145	151	PF00069	0.314
MOD_CK1_1	323	329	PF00069	0.575
MOD_CK1_1	514	520	PF00069	0.627
MOD_CK1_1	94	100	PF00069	0.382
MOD_CK2_1	27	33	PF00069	0.739
MOD_CK2_1	364	370	PF00069	0.406
MOD_CK2_1	380	386	PF00069	0.277
MOD_CK2_1	9	15	PF00069	0.726
MOD_CMANNOS	390	393	PF00535	0.277
MOD_DYRK1A_RPxSP_1	167	171	PF00069	0.349
MOD_GlcNHglycan	130	133	PF01048	0.378
MOD_GlcNHglycan	148	151	PF01048	0.222
MOD_GlcNHglycan	287	290	PF01048	0.375
MOD_GlcNHglycan	373	376	PF01048	0.406
MOD_GlcNHglycan	437	440	PF01048	0.312
MOD_GlcNHglycan	460	463	PF01048	0.387
MOD_GlcNHglycan	507	510	PF01048	0.733
MOD_GlcNHglycan	516	519	PF01048	0.672
MOD_GlcNHglycan	74	77	PF01048	0.477
MOD_GSK3_1	142	149	PF00069	0.347
MOD_GSK3_1	150	157	PF00069	0.294
MOD_GSK3_1	18	25	PF00069	0.699
MOD_GSK3_1	323	330	PF00069	0.461
MOD_GSK3_1	454	461	PF00069	0.439
MOD_GSK3_1	60	67	PF00069	0.788
MOD_GSK3_1	90	97	PF00069	0.362
MOD_N-GLC_1	284	289	PF02516	0.439
MOD_N-GLC_1	94	99	PF02516	0.423
MOD_NEK2_1	293	298	PF00069	0.497
MOD_PIKK_1	217	223	PF00454	0.277
MOD_PIKK_1	452	458	PF00454	0.212
MOD_PKA_1	230	236	PF00069	0.277
MOD_PKA_2	100	106	PF00069	0.390
MOD_PKA_2	285	291	PF00069	0.448
MOD_PKA_2	334	340	PF00069	0.535
MOD_Plk_2-3	22	28	PF00069	0.801
MOD_ProDKin_1	139	145	PF00069	0.303
MOD_ProDKin_1	167	173	PF00069	0.316
MOD_ProDKin_1	202	208	PF00069	0.324
MOD_ProDKin_1	223	229	PF00069	0.277
MOD_SUMO_for_1	207	210	PF00179	0.355
TRG_ENDOCYTIC_2	186	189	PF00928	0.413
TRG_ENDOCYTIC_2	463	466	PF00928	0.357
TRG_ER_diArg_1	127	129	PF00400	0.295
TRG_ER_diArg_1	529	531	PF00400	0.626
TRG_NES_CRM1_1	212	225	PF08389	0.295
TRG_Pf-PMV_PEXEL_1	327	332	PF00026	0.421
TRG_Pf-PMV_PEXEL_1	377	381	PF00026	0.409

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I0R5	Leptomonas seymouri	66%	77%
A0A0S4IY41	Bodo saltans	45%	100%
A0A1X0NXL3	Trypanosomatidae	53%	85%
A0A3S7WXR7	Leishmania donovani	93%	100%
A4HCT9	Leishmania braziliensis	81%	100%
A4I0B9	Leishmania infantum	93%	78%
C9ZV17	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	62%	85%
E9AW83	Leishmania mexicana (strain MHOM/GT/2001/U1103)	90%	99%
V5D725	Trypanosoma cruzi	62%	85%