5-formyltetrahydrofolate cyclo-ligase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

5-formyltetrahydrofolate cyclo-ligase family, putative

Species:

Leishmania major

UniProt:

Q4QB50_LEIMA

TriTrypDb:

LmjF.23.1045 , LMJLV39_230019900 , LMJSD75_230020200

Length:

449

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005739	mitochondrion	5	2
GO:0043226	organelle	2	2
GO:0043227	membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043231	intracellular membrane-bounded organelle	4	2
GO:0110165	cellular anatomical entity	1	2

Expansion

Sequence features

Q4QB50

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4QB50

Function

Biological processes
Term	Name	Level	Count
GO:0006575	cellular modified amino acid metabolic process	3	2
GO:0006725	cellular aromatic compound metabolic process	3	2
GO:0006730	one-carbon metabolic process	3	2
GO:0006760	folic acid-containing compound metabolic process	4	2
GO:0006807	nitrogen compound metabolic process	2	2
GO:0008152	metabolic process	1	2
GO:0009058	biosynthetic process	2	2
GO:0009396	folic acid-containing compound biosynthetic process	5	2
GO:0009987	cellular process	1	2
GO:0018130	heterocycle biosynthetic process	4	2
GO:0019438	aromatic compound biosynthetic process	4	2
GO:0034641	cellular nitrogen compound metabolic process	3	2
GO:0035999	tetrahydrofolate interconversion	4	2
GO:0042398	cellular modified amino acid biosynthetic process	4	2
GO:0042558	pteridine-containing compound metabolic process	4	2
GO:0042559	pteridine-containing compound biosynthetic process	5	2
GO:0044237	cellular metabolic process	2	2
GO:0044249	cellular biosynthetic process	3	2
GO:0044271	cellular nitrogen compound biosynthetic process	4	2
GO:0044281	small molecule metabolic process	2	2
GO:0046483	heterocycle metabolic process	3	2
GO:0046653	tetrahydrofolate metabolic process	5	2
GO:0071704	organic substance metabolic process	2	2
GO:1901360	organic cyclic compound metabolic process	3	2
GO:1901362	organic cyclic compound biosynthetic process	4	2
GO:1901564	organonitrogen compound metabolic process	3	2
GO:1901566	organonitrogen compound biosynthetic process	4	2
GO:1901576	organic substance biosynthetic process	3	2

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	3
GO:0016874	ligase activity	2	3
GO:0016879	ligase activity, forming carbon-nitrogen bonds	3	2
GO:0016882	cyclo-ligase activity	4	2
GO:0030272	5-formyltetrahydrofolate cyclo-ligase activity	5	2

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_MEL_PAP_1	353	359	PF00089	0.550
CLV_NRD_NRD_1	13	15	PF00675	0.342
CLV_NRD_NRD_1	22	24	PF00675	0.348
CLV_PCSK_FUR_1	20	24	PF00082	0.415
CLV_PCSK_KEX2_1	22	24	PF00082	0.406
CLV_PCSK_SKI1_1	10	14	PF00082	0.367
CLV_PCSK_SKI1_1	166	170	PF00082	0.381
CLV_PCSK_SKI1_1	196	200	PF00082	0.407
CLV_PCSK_SKI1_1	23	27	PF00082	0.414
DEG_Nend_UBRbox_3	1	3	PF02207	0.551
DEG_SPOP_SBC_1	148	152	PF00917	0.612
DOC_ANK_TNKS_1	211	218	PF00023	0.421
DOC_CKS1_1	118	123	PF01111	0.454
DOC_CYCLIN_yCln2_LP_2	157	160	PF00134	0.523
DOC_CYCLIN_yCln2_LP_2	202	208	PF00134	0.477
DOC_MAPK_DCC_7	80	89	PF00069	0.273
DOC_MAPK_gen_1	10	21	PF00069	0.499
DOC_MAPK_gen_1	166	175	PF00069	0.425
DOC_MAPK_MEF2A_6	169	177	PF00069	0.470
DOC_MAPK_MEF2A_6	80	89	PF00069	0.273
DOC_MAPK_RevD_3	7	23	PF00069	0.411
DOC_PP2B_LxvP_1	157	160	PF13499	0.519
DOC_PP2B_LxvP_1	81	84	PF13499	0.429
DOC_PP4_FxxP_1	262	265	PF00568	0.346
DOC_USP7_MATH_1	148	152	PF00917	0.645
DOC_USP7_MATH_1	300	304	PF00917	0.484
DOC_USP7_MATH_1	349	353	PF00917	0.550
DOC_USP7_MATH_1	365	369	PF00917	0.652
DOC_USP7_UBL2_3	11	15	PF12436	0.495
DOC_WW_Pin1_4	117	122	PF00397	0.394
DOC_WW_Pin1_4	142	147	PF00397	0.733
DOC_WW_Pin1_4	221	226	PF00397	0.595
DOC_WW_Pin1_4	287	292	PF00397	0.606
DOC_WW_Pin1_4	434	439	PF00397	0.419
DOC_WW_Pin1_4	79	84	PF00397	0.556
LIG_14-3-3_CanoR_1	166	175	PF00244	0.356
LIG_14-3-3_CanoR_1	23	29	PF00244	0.447
LIG_14-3-3_CanoR_1	60	69	PF00244	0.618
LIG_Actin_WH2_2	386	403	PF00022	0.338
LIG_AP2alpha_1	332	336	PF02296	0.483
LIG_APCC_ABBA_1	92	97	PF00400	0.322
LIG_BIR_III_2	374	378	PF00653	0.483
LIG_eIF4E_1	270	276	PF01652	0.511
LIG_FHA_1	118	124	PF00498	0.498
LIG_HCF-1_HBM_1	41	44	PF13415	0.445
LIG_LIR_Apic_2	426	431	PF02991	0.313
LIG_LIR_Gen_1	256	265	PF02991	0.355
LIG_LIR_Gen_1	41	51	PF02991	0.303
LIG_LIR_Nem_3	256	261	PF02991	0.353
LIG_LIR_Nem_3	334	339	PF02991	0.464
LIG_LIR_Nem_3	41	47	PF02991	0.327
LIG_Pex14_2	332	336	PF04695	0.483
LIG_SH2_CRK	258	262	PF00017	0.410
LIG_SH2_CRK	428	432	PF00017	0.293
LIG_SH2_SRC	44	47	PF00017	0.427
LIG_SH2_STAP1	258	262	PF00017	0.410
LIG_SH2_STAP1	333	337	PF00017	0.436
LIG_SH2_STAP1	44	48	PF00017	0.440
LIG_SH2_STAT5	46	49	PF00017	0.400
LIG_SH2_STAT5	91	94	PF00017	0.341
LIG_SH2_STAT5	95	98	PF00017	0.341
LIG_SH3_3	119	125	PF00018	0.364
LIG_SH3_3	157	163	PF00018	0.711
LIG_SH3_3	207	213	PF00018	0.348
LIG_SH3_3	308	314	PF00018	0.498
LIG_SH3_3	370	376	PF00018	0.536
LIG_SH3_3	380	386	PF00018	0.515
LIG_SH3_3	432	438	PF00018	0.382
LIG_SUMO_SIM_par_1	120	127	PF11976	0.361
LIG_SUMO_SIM_par_1	138	143	PF11976	0.507
LIG_TYR_ITIM	89	94	PF00017	0.357
LIG_UBA3_1	18	26	PF00899	0.489
MOD_CK1_1	152	158	PF00069	0.740
MOD_CK1_1	211	217	PF00069	0.567
MOD_CK1_1	224	230	PF00069	0.629
MOD_CK1_1	352	358	PF00069	0.561
MOD_CK1_1	402	408	PF00069	0.613
MOD_CK1_1	415	421	PF00069	0.600
MOD_CK1_1	54	60	PF00069	0.593
MOD_CK1_1	63	69	PF00069	0.641
MOD_CK2_1	198	204	PF00069	0.543
MOD_CK2_1	223	229	PF00069	0.662
MOD_GlcNHglycan	169	172	PF01048	0.359
MOD_GlcNHglycan	213	216	PF01048	0.503
MOD_GlcNHglycan	218	221	PF01048	0.503
MOD_GlcNHglycan	272	275	PF01048	0.482
MOD_GlcNHglycan	380	383	PF01048	0.489
MOD_GlcNHglycan	407	410	PF01048	0.517
MOD_GlcNHglycan	438	441	PF01048	0.482
MOD_GlcNHglycan	53	56	PF01048	0.573
MOD_GSK3_1	126	133	PF00069	0.489
MOD_GSK3_1	138	145	PF00069	0.605
MOD_GSK3_1	148	155	PF00069	0.705
MOD_GSK3_1	279	286	PF00069	0.526
MOD_GSK3_1	400	407	PF00069	0.588
MOD_GSK3_1	414	421	PF00069	0.621
MOD_GSK3_1	59	66	PF00069	0.542
MOD_NEK2_1	198	203	PF00069	0.474
MOD_NEK2_1	241	246	PF00069	0.613
MOD_NEK2_1	378	383	PF00069	0.487
MOD_NEK2_1	400	405	PF00069	0.450
MOD_NEK2_2	339	344	PF00069	0.522
MOD_PIKK_1	33	39	PF00454	0.497
MOD_PIKK_1	69	75	PF00454	0.627
MOD_PKA_2	211	217	PF00069	0.508
MOD_PKA_2	270	276	PF00069	0.560
MOD_PKA_2	400	406	PF00069	0.657
MOD_PKA_2	51	57	PF00069	0.452
MOD_PKA_2	59	65	PF00069	0.536
MOD_Plk_1	305	311	PF00069	0.519
MOD_Plk_1	415	421	PF00069	0.508
MOD_Plk_4	149	155	PF00069	0.527
MOD_Plk_4	198	204	PF00069	0.507
MOD_Plk_4	305	311	PF00069	0.531
MOD_ProDKin_1	117	123	PF00069	0.403
MOD_ProDKin_1	142	148	PF00069	0.553
MOD_ProDKin_1	221	227	PF00069	0.596
MOD_ProDKin_1	287	293	PF00069	0.351
MOD_ProDKin_1	434	440	PF00069	0.422
MOD_ProDKin_1	79	85	PF00069	0.542
MOD_SUMO_for_1	25	28	PF00179	0.434
TRG_DiLeu_BaLyEn_6	82	87	PF01217	0.460
TRG_ENDOCYTIC_2	258	261	PF00928	0.416
TRG_ENDOCYTIC_2	333	336	PF00928	0.436
TRG_ENDOCYTIC_2	44	47	PF00928	0.324
TRG_ENDOCYTIC_2	91	94	PF00928	0.301
TRG_ENDOCYTIC_2	95	98	PF00928	0.294
TRG_ER_diArg_1	19	22	PF00400	0.411
TRG_ER_diArg_1	345	348	PF00400	0.505

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I1K4	Leptomonas seymouri	42%	100%
A0A1X0NWU1	Trypanosomatidae	28%	100%
A0A3Q8IEK9	Leishmania donovani	90%	100%
A0A3R7KPU1	Trypanosoma rangeli	27%	100%
A4HCX6	Leishmania braziliensis	71%	100%
A4I0F9	Leishmania infantum	90%	100%
C9ZV81	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	34%	100%
E9AWC2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	85%	100%
V5DFL5	Trypanosoma cruzi	29%	100%