Putative Qb-SNARE protein

Quick info Annotations Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Qb-SNARE protein, putative

Species:

Leishmania major

UniProt:

Q4QAZ8_LEIMA

TriTrypDb:

LmjF.23.1750 , LMJLV39_230025700 , LMJSD75_230026300

Length:

338

Annotations

LeishMANIAdb annotations

Similar to other Eukaryotic vesicle-associated V-SNARE proteins, especially VTI1.. Localization: Vesicular (by homology)

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 9

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 1, no: 18
NetGPI	no	yes: 0, no: 19

Cellular components
Term	Name	Level	Count
GO:0005789	endoplasmic reticulum membrane	4	3
GO:0005794	Golgi apparatus	5	6
GO:0010008	endosome membrane	5	3
GO:0012506	vesicle membrane	4	3
GO:0012507	ER to Golgi transport vesicle membrane	6	3
GO:0016020	membrane	2	20
GO:0030658	transport vesicle membrane	5	3
GO:0030659	cytoplasmic vesicle membrane	5	3
GO:0030662	coated vesicle membrane	5	3
GO:0031090	organelle membrane	3	3
GO:0031201	SNARE complex	3	3
GO:0031902	late endosome membrane	6	3
GO:0032991	protein-containing complex	1	3
GO:0043226	organelle	2	12
GO:0043227	membrane-bounded organelle	3	12
GO:0043229	intracellular organelle	3	12
GO:0043231	intracellular membrane-bounded organelle	4	12
GO:0098588	bounding membrane of organelle	4	3
GO:0098796	membrane protein complex	2	3
GO:0110165	cellular anatomical entity	1	20
GO:0005737	cytoplasm	2	15

Expansion

Sequence features

Q4QAZ8

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4QAZ8

Function

Biological processes
Term	Name	Level	Count
GO:0006810	transport	3	20
GO:0006886	intracellular protein transport	4	15
GO:0006906	vesicle fusion	6	3
GO:0006996	organelle organization	4	3
GO:0008104	protein localization	4	20
GO:0009987	cellular process	1	20
GO:0015031	protein transport	4	20
GO:0016043	cellular component organization	3	3
GO:0016050	vesicle organization	5	3
GO:0033036	macromolecule localization	2	20
GO:0045184	establishment of protein localization	3	20
GO:0046907	intracellular transport	3	15
GO:0048284	organelle fusion	5	3
GO:0051179	localization	1	20
GO:0051234	establishment of localization	2	20
GO:0051641	cellular localization	2	20
GO:0051649	establishment of localization in cell	3	15
GO:0061024	membrane organization	4	3
GO:0061025	membrane fusion	5	3
GO:0070727	cellular macromolecule localization	3	20
GO:0071702	organic substance transport	4	20
GO:0071705	nitrogen compound transport	4	20
GO:0071840	cellular component organization or biogenesis	2	3
GO:0090174	organelle membrane fusion	6	3
GO:0016192	vesicle-mediated transport	4	11
GO:0006890	retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum	6	2
GO:0048193	Golgi vesicle transport	5	2

Molecular functions
Term	Name	Level	Count
GO:0000149	SNARE binding	3	3
GO:0005484	SNAP receptor activity	3	8
GO:0005488	binding	1	3
GO:0005515	protein binding	2	3
GO:0030674	protein-macromolecule adaptor activity	2	8
GO:0060090	molecular adaptor activity	1	8

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	262	266	PF00656	0.603
CLV_C14_Caspase3-7	67	71	PF00656	0.628
CLV_NRD_NRD_1	178	180	PF00675	0.549
CLV_NRD_NRD_1	267	269	PF00675	0.391
CLV_NRD_NRD_1	5	7	PF00675	0.349
CLV_NRD_NRD_1	87	89	PF00675	0.337
CLV_PCSK_KEX2_1	19	21	PF00082	0.359
CLV_PCSK_KEX2_1	267	269	PF00082	0.347
CLV_PCSK_KEX2_1	5	7	PF00082	0.349
CLV_PCSK_PC1ET2_1	19	21	PF00082	0.363
CLV_PCSK_SKI1_1	281	285	PF00082	0.389
DEG_APCC_DBOX_1	147	155	PF00400	0.528
DEG_APCC_DBOX_1	33	41	PF00400	0.517
DOC_CDC14_PxL_1	38	46	PF14671	0.495
DOC_MAPK_gen_1	5	13	PF00069	0.554
DOC_MAPK_JIP1_4	34	40	PF00069	0.517
DOC_PP4_MxPP_1	1	4	PF00568	0.545
DOC_USP7_MATH_1	163	167	PF00917	0.607
DOC_USP7_MATH_1	238	242	PF00917	0.569
DOC_USP7_MATH_1	28	32	PF00917	0.549
DOC_USP7_MATH_1	321	325	PF00917	0.550
DOC_USP7_MATH_1	4	8	PF00917	0.547
DOC_USP7_UBL2_3	275	279	PF12436	0.622
LIG_14-3-3_CanoR_1	137	144	PF00244	0.548
LIG_14-3-3_CanoR_1	243	252	PF00244	0.610
LIG_14-3-3_CanoR_1	5	13	PF00244	0.550
LIG_Actin_WH2_2	68	85	PF00022	0.587
LIG_BIR_III_4	216	220	PF00653	0.517
LIG_FHA_1	110	116	PF00498	0.504
LIG_FHA_1	229	235	PF00498	0.559
LIG_FHA_1	253	259	PF00498	0.667
LIG_FHA_1	283	289	PF00498	0.424
LIG_FHA_2	137	143	PF00498	0.540
LIG_FHA_2	151	157	PF00498	0.436
LIG_FHA_2	311	317	PF00498	0.500
LIG_FHA_2	72	78	PF00498	0.608
LIG_LIR_Nem_3	58	64	PF02991	0.583
LIG_SH2_CRK	93	97	PF00017	0.497
LIG_SH2_PTP2	300	303	PF00017	0.384
LIG_SH2_STAP1	64	68	PF00017	0.549
LIG_SH2_STAT5	300	303	PF00017	0.347
LIG_SH3_3	22	28	PF00018	0.563
LIG_SH3_3	95	101	PF00018	0.450
LIG_SUMO_SIM_anti_2	169	176	PF11976	0.603
LIG_SUMO_SIM_anti_2	285	291	PF11976	0.469
LIG_SUMO_SIM_anti_2	36	42	PF11976	0.628
LIG_SUMO_SIM_par_1	204	209	PF11976	0.669
LIG_SUMO_SIM_par_1	254	262	PF11976	0.661
LIG_TRAF2_1	153	156	PF00917	0.553
LIG_TRAF2_1	63	66	PF00917	0.580
LIG_UBA3_1	173	180	PF00899	0.654
LIG_UBA3_1	269	275	PF00899	0.595
LIG_WW_3	2	6	PF00397	0.408
MOD_CK1_1	23	29	PF00069	0.454
MOD_CK1_1	310	316	PF00069	0.740
MOD_CK1_1	330	336	PF00069	0.623
MOD_CK1_1	36	42	PF00069	0.364
MOD_CK2_1	136	142	PF00069	0.356
MOD_CK2_1	150	156	PF00069	0.253
MOD_CK2_1	310	316	PF00069	0.732
MOD_GlcNHglycan	164	168	PF01048	0.506
MOD_GlcNHglycan	182	185	PF01048	0.508
MOD_GlcNHglycan	270	273	PF01048	0.469
MOD_GSK3_1	146	153	PF00069	0.383
MOD_GSK3_1	178	185	PF00069	0.519
MOD_GSK3_1	307	314	PF00069	0.638
MOD_GSK3_1	321	328	PF00069	0.445
MOD_GSK3_1	96	103	PF00069	0.336
MOD_N-GLC_1	228	233	PF02516	0.459
MOD_N-GLC_1	308	313	PF02516	0.716
MOD_NEK2_1	208	213	PF00069	0.488
MOD_NEK2_1	282	287	PF00069	0.367
MOD_NEK2_2	238	243	PF00069	0.626
MOD_NEK2_2	4	9	PF00069	0.429
MOD_NEK2_2	56	61	PF00069	0.348
MOD_NEK2_2	71	76	PF00069	0.372
MOD_PKA_1	179	185	PF00069	0.596
MOD_PKA_2	136	142	PF00069	0.402
MOD_PKA_2	178	184	PF00069	0.703
MOD_PKA_2	23	29	PF00069	0.445
MOD_PKA_2	307	313	PF00069	0.526
MOD_PKA_2	33	39	PF00069	0.384
MOD_PKA_2	4	10	PF00069	0.420
MOD_PKA_2	82	88	PF00069	0.328
MOD_Plk_1	253	259	PF00069	0.589
MOD_Plk_4	110	116	PF00069	0.403
MOD_Plk_4	253	259	PF00069	0.520
MOD_Plk_4	28	34	PF00069	0.500
MOD_Plk_4	327	333	PF00069	0.661
MOD_Plk_4	36	42	PF00069	0.397
MOD_Plk_4	56	62	PF00069	0.505
MOD_SUMO_rev_2	172	182	PF00179	0.557
MOD_SUMO_rev_2	85	91	PF00179	0.471
TRG_DiLeu_BaLyEn_6	223	228	PF01217	0.494
TRG_DiLeu_BaLyEn_6	39	44	PF01217	0.340
TRG_ENDOCYTIC_2	300	303	PF00928	0.399
TRG_ER_diArg_1	267	269	PF00400	0.540
TRG_ER_diArg_1	4	6	PF00400	0.414
TRG_Pf-PMV_PEXEL_1	152	156	PF00026	0.326
TRG_Pf-PMV_PEXEL_1	226	230	PF00026	0.447
TRG_Pf-PMV_PEXEL_1	73	77	PF00026	0.593

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P6G6	Leptomonas seymouri	72%	100%
A0A0N1HXG1	Leptomonas seymouri	32%	100%
A0A0S4IRD2	Bodo saltans	34%	100%
A0A1X0NY79	Trypanosomatidae	51%	100%
A0A3Q8IEP1	Leishmania donovani	36%	100%
A0A3S5IRB0	Trypanosoma rangeli	50%	100%
A0A3S7WY13	Leishmania donovani	90%	97%
A0A422NJV1	Trypanosoma rangeli	34%	100%
A4HD24	Leishmania braziliensis	36%	100%
A4HD25	Leishmania braziliensis	81%	100%
A4I0L1	Leishmania infantum	36%	100%
A4I0L2	Leishmania infantum	93%	100%
C9ZVI1	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	35%	100%
E9AWH2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	36%	100%
E9AWH3	Leishmania mexicana (strain MHOM/GT/2001/U1103)	89%	100%
Q4QAZ9	Leishmania major	36%	100%
V5ARB2	Trypanosoma cruzi	49%	100%
V5BFR7	Trypanosoma cruzi	34%	100%