GAF domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

GAF domain/TIP41-like family, putative

Species:

Leishmania major

UniProt:

Q4QAZ2_LEIMA

TriTrypDb:

LmjF.23.1460 , LMJLV39_230026300 , LMJSD75_230026900

Length:

523

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	2
GO:0005829	cytosol	2	2
GO:0110165	cellular anatomical entity	1	2

Expansion

Sequence features

Q4QAZ2

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4QAZ2

Function

Biological processes
Term	Name	Level	Count
GO:0010921	regulation of phosphatase activity	5	11
GO:0019220	regulation of phosphate metabolic process	6	11
GO:0019222	regulation of metabolic process	3	11
GO:0031323	regulation of cellular metabolic process	4	11
GO:0031399	regulation of protein modification process	6	11
GO:0035303	regulation of dephosphorylation	7	11
GO:0035304	regulation of protein dephosphorylation	7	11
GO:0043666	regulation of phosphoprotein phosphatase activity	6	11
GO:0050789	regulation of biological process	2	11
GO:0050790	regulation of catalytic activity	3	11
GO:0050794	regulation of cellular process	3	11
GO:0051171	regulation of nitrogen compound metabolic process	4	11
GO:0051174	regulation of phosphorus metabolic process	5	11
GO:0051246	regulation of protein metabolic process	5	11
GO:0051336	regulation of hydrolase activity	4	11
GO:0060255	regulation of macromolecule metabolic process	4	11
GO:0065007	biological regulation	1	11
GO:0065009	regulation of molecular function	2	11
GO:0080090	regulation of primary metabolic process	4	11

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	2
GO:0016491	oxidoreductase activity	2	2
GO:0016667	oxidoreductase activity, acting on a sulfur group of donors	3	2
GO:0016671	oxidoreductase activity, acting on a sulfur group of donors, disulfide as acceptor	4	2
GO:0070191	methionine-R-sulfoxide reductase activity	5	2

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	386	390	PF00656	0.478
CLV_PCSK_KEX2_1	34	36	PF00082	0.539
CLV_PCSK_PC1ET2_1	34	36	PF00082	0.539
CLV_PCSK_SKI1_1	272	276	PF00082	0.405
CLV_PCSK_SKI1_1	34	38	PF00082	0.485
CLV_PCSK_SKI1_1	519	523	PF00082	0.637
DEG_APCC_DBOX_1	168	176	PF00400	0.314
DEG_APCC_DBOX_1	266	274	PF00400	0.428
DEG_SCF_FBW7_1	222	229	PF00400	0.525
DEG_SCF_FBW7_1	3	10	PF00400	0.486
DEG_SPOP_SBC_1	226	230	PF00917	0.711
DOC_CDC14_PxL_1	79	87	PF14671	0.411
DOC_CKS1_1	223	228	PF01111	0.529
DOC_CYCLIN_yCln2_LP_2	342	348	PF00134	0.478
DOC_MAPK_DCC_7	467	477	PF00069	0.417
DOC_MAPK_MEF2A_6	136	145	PF00069	0.411
DOC_MAPK_MEF2A_6	309	316	PF00069	0.438
DOC_MAPK_MEF2A_6	335	344	PF00069	0.462
DOC_MAPK_MEF2A_6	399	407	PF00069	0.456
DOC_PP1_RVXF_1	359	365	PF00149	0.474
DOC_PP1_RVXF_1	69	75	PF00149	0.456
DOC_PP2B_LxvP_1	197	200	PF13499	0.532
DOC_PP2B_LxvP_1	342	345	PF13499	0.445
DOC_PP4_FxxP_1	60	63	PF00568	0.446
DOC_PP4_FxxP_1	80	83	PF00568	0.145
DOC_USP7_MATH_1	130	134	PF00917	0.281
DOC_USP7_UBL2_3	247	251	PF12436	0.549
DOC_USP7_UBL2_3	357	361	PF12436	0.478
DOC_WW_Pin1_4	222	227	PF00397	0.719
DOC_WW_Pin1_4	230	235	PF00397	0.532
DOC_WW_Pin1_4	3	8	PF00397	0.600
DOC_WW_Pin1_4	301	306	PF00397	0.477
DOC_WW_Pin1_4	34	39	PF00397	0.429
DOC_WW_Pin1_4	465	470	PF00397	0.537
DOC_WW_Pin1_4	496	501	PF00397	0.439
LIG_14-3-3_CanoR_1	343	353	PF00244	0.562
LIG_14-3-3_CanoR_1	431	441	PF00244	0.522
LIG_14-3-3_CanoR_1	94	102	PF00244	0.372
LIG_Actin_WH2_2	469	484	PF00022	0.396
LIG_APCC_ABBA_1	212	217	PF00400	0.745
LIG_APCC_ABBA_1	457	462	PF00400	0.456
LIG_BIR_III_4	389	393	PF00653	0.478
LIG_BRCT_BRCA1_1	32	36	PF00533	0.530
LIG_BRCT_BRCA1_1	351	355	PF00533	0.478
LIG_BRCT_BRCA1_1	433	437	PF00533	0.552
LIG_BRCT_BRCA1_2	351	357	PF00533	0.478
LIG_deltaCOP1_diTrp_1	310	316	PF00928	0.492
LIG_deltaCOP1_diTrp_1	363	370	PF00928	0.431
LIG_deltaCOP1_diTrp_1	451	460	PF00928	0.446
LIG_EH1_1	315	323	PF00400	0.455
LIG_FHA_1	255	261	PF00498	0.420
LIG_FHA_1	277	283	PF00498	0.362
LIG_FHA_1	298	304	PF00498	0.410
LIG_FHA_1	399	405	PF00498	0.380
LIG_FHA_1	423	429	PF00498	0.522
LIG_FHA_1	47	53	PF00498	0.454
LIG_FHA_1	506	512	PF00498	0.407
LIG_FHA_2	107	113	PF00498	0.331
LIG_FHA_2	266	272	PF00498	0.419
LIG_FHA_2	273	279	PF00498	0.390
LIG_FXI_DFP_1	182	186	PF00024	0.393
LIG_FXI_DFP_1	408	412	PF00024	0.278
LIG_IRF3_LxIS_1	282	289	PF10401	0.441
LIG_LIR_Gen_1	16	25	PF02991	0.304
LIG_LIR_Gen_1	310	321	PF02991	0.438
LIG_LIR_Gen_1	347	358	PF02991	0.464
LIG_LIR_Gen_1	410	418	PF02991	0.478
LIG_LIR_Gen_1	435	445	PF02991	0.465
LIG_LIR_Nem_3	16	21	PF02991	0.300
LIG_LIR_Nem_3	310	316	PF02991	0.446
LIG_LIR_Nem_3	331	337	PF02991	0.470
LIG_LIR_Nem_3	347	353	PF02991	0.396
LIG_LIR_Nem_3	434	440	PF02991	0.472
LIG_MLH1_MIPbox_1	434	438	PF16413	0.446
LIG_Pex14_1	366	370	PF04695	0.431
LIG_Pex14_1	74	78	PF04695	0.266
LIG_Pex14_2	407	411	PF04695	0.456
LIG_Pex14_2	437	441	PF04695	0.491
LIG_REV1ctd_RIR_1	243	251	PF16727	0.557
LIG_SH2_CRK	202	206	PF00017	0.668
LIG_SH2_STAP1	18	22	PF00017	0.395
LIG_SH2_STAP1	202	206	PF00017	0.668
LIG_SH2_STAT3	78	81	PF00017	0.300
LIG_SH2_STAT5	78	81	PF00017	0.279
LIG_SH3_3	247	253	PF00018	0.461
LIG_SH3_3	378	384	PF00018	0.479
LIG_SUMO_SIM_anti_2	149	155	PF11976	0.331
LIG_SUMO_SIM_anti_2	400	407	PF11976	0.415
LIG_SUMO_SIM_par_1	141	146	PF11976	0.337
LIG_SUMO_SIM_par_1	149	155	PF11976	0.252
LIG_SUMO_SIM_par_1	299	304	PF11976	0.459
LIG_SUMO_SIM_par_1	507	512	PF11976	0.374
LIG_SxIP_EBH_1	236	247	PF03271	0.589
MOD_CK1_1	113	119	PF00069	0.381
MOD_CK1_1	304	310	PF00069	0.573
MOD_CK2_1	10	16	PF00069	0.393
MOD_CK2_1	106	112	PF00069	0.411
MOD_CK2_1	118	124	PF00069	0.281
MOD_CK2_1	132	138	PF00069	0.203
MOD_CK2_1	272	278	PF00069	0.434
MOD_CK2_1	304	310	PF00069	0.509
MOD_CK2_1	321	327	PF00069	0.278
MOD_GlcNHglycan	112	115	PF01048	0.364
MOD_GlcNHglycan	132	135	PF01048	0.230
MOD_GlcNHglycan	202	205	PF01048	0.595
MOD_GlcNHglycan	288	291	PF01048	0.443
MOD_GlcNHglycan	323	326	PF01048	0.343
MOD_GlcNHglycan	346	349	PF01048	0.381
MOD_GlcNHglycan	434	437	PF01048	0.430
MOD_GlcNHglycan	469	472	PF01048	0.456
MOD_GlcNHglycan	503	506	PF01048	0.517
MOD_GSK3_1	106	113	PF00069	0.435
MOD_GSK3_1	130	137	PF00069	0.297
MOD_GSK3_1	222	229	PF00069	0.691
MOD_GSK3_1	272	279	PF00069	0.409
MOD_GSK3_1	282	289	PF00069	0.480
MOD_GSK3_1	297	304	PF00069	0.390
MOD_GSK3_1	3	10	PF00069	0.605
MOD_GSK3_1	30	37	PF00069	0.484
MOD_GSK3_1	463	470	PF00069	0.468
MOD_GSK3_1	501	508	PF00069	0.539
MOD_N-GLC_1	146	151	PF02516	0.411
MOD_N-GLC_2	369	371	PF02516	0.287
MOD_NEK2_1	265	270	PF00069	0.429
MOD_NEK2_1	282	287	PF00069	0.364
MOD_NEK2_1	321	326	PF00069	0.412
MOD_NEK2_1	398	403	PF00069	0.430
MOD_NEK2_1	423	428	PF00069	0.338
MOD_NEK2_1	46	51	PF00069	0.404
MOD_NEK2_1	463	468	PF00069	0.309
MOD_NEK2_2	349	354	PF00069	0.287
MOD_PIKK_1	272	278	PF00454	0.404
MOD_PK_1	299	305	PF00069	0.359
MOD_Plk_1	399	405	PF00069	0.309
MOD_Plk_4	113	119	PF00069	0.264
MOD_Plk_4	146	152	PF00069	0.411
MOD_Plk_4	333	339	PF00069	0.480
MOD_Plk_4	399	405	PF00069	0.386
MOD_Plk_4	505	511	PF00069	0.435
MOD_ProDKin_1	222	228	PF00069	0.718
MOD_ProDKin_1	230	236	PF00069	0.529
MOD_ProDKin_1	3	9	PF00069	0.585
MOD_ProDKin_1	301	307	PF00069	0.488
MOD_ProDKin_1	34	40	PF00069	0.424
MOD_ProDKin_1	465	471	PF00069	0.415
MOD_ProDKin_1	496	502	PF00069	0.435
MOD_SUMO_for_1	246	249	PF00179	0.567
MOD_SUMO_rev_2	277	282	PF00179	0.517
MOD_SUMO_rev_2	327	337	PF00179	0.385
MOD_SUMO_rev_2	356	362	PF00179	0.421
MOD_SUMO_rev_2	488	498	PF00179	0.544
TRG_DiLeu_BaEn_1	333	338	PF01217	0.520
TRG_ENDOCYTIC_2	18	21	PF00928	0.318
TRG_ENDOCYTIC_2	202	205	PF00928	0.662
TRG_ENDOCYTIC_2	408	411	PF00928	0.411
TRG_ENDOCYTIC_2	430	433	PF00928	0.331
TRG_ER_diArg_1	377	380	PF00400	0.350

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I5N3	Leptomonas seymouri	68%	100%
A0A0S4JL71	Bodo saltans	35%	100%
A0A1X0NWX3	Trypanosomatidae	44%	97%
A0A3S7WY26	Leishmania donovani	95%	100%
A0A422NJH6	Trypanosoma rangeli	43%	94%
A4HD30	Leishmania braziliensis	83%	100%
A4I0L7	Leishmania infantum	95%	100%
C9ZNQ7	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	41%	91%
E9AWH8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	94%	100%
V5BB88	Trypanosoma cruzi	45%	95%