Putative clathrin coat assembly protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

clathrin coat assembly protein, putative

Species:

Leishmania major

UniProt:

Q4QAX8_LEIMA

TriTrypDb:

LmjF.24.0020 , LMJLV39_240005500 , LMJSD75_240005200

Length:

483

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0000331	contractile vacuole	6	2
GO:0005737	cytoplasm	2	2
GO:0005773	vacuole	5	2
GO:0005905	clathrin-coated pit	4	2
GO:0016020	membrane	2	2
GO:0030135	coated vesicle	7	12
GO:0030136	clathrin-coated vesicle	8	12
GO:0031410	cytoplasmic vesicle	6	12
GO:0031982	vesicle	4	12
GO:0043226	organelle	2	12
GO:0043227	membrane-bounded organelle	3	12
GO:0043229	intracellular organelle	3	12
GO:0043231	intracellular membrane-bounded organelle	4	12
GO:0097708	intracellular vesicle	5	12
GO:0098590	plasma membrane region	3	2
GO:0110165	cellular anatomical entity	1	12

Expansion

Sequence features

Q4QAX8

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4QAX8

Function

Biological processes
Term	Name	Level	Count
GO:0006810	transport	3	12
GO:0006897	endocytosis	5	12
GO:0006898	receptor-mediated endocytosis	6	12
GO:0006900	vesicle budding from membrane	5	2
GO:0006996	organelle organization	4	2
GO:0009987	cellular process	1	12
GO:0016043	cellular component organization	3	12
GO:0016050	vesicle organization	5	2
GO:0016192	vesicle-mediated transport	4	12
GO:0022607	cellular component assembly	4	12
GO:0043933	protein-containing complex organization	4	12
GO:0048268	clathrin coat assembly	6	12
GO:0051179	localization	1	12
GO:0051234	establishment of localization	2	12
GO:0061024	membrane organization	4	2
GO:0065003	protein-containing complex assembly	5	12
GO:0071840	cellular component organization or biogenesis	2	12
GO:0072583	clathrin-dependent endocytosis	7	12

Molecular functions
Term	Name	Level	Count
GO:0000149	SNARE binding	3	2
GO:0005488	binding	1	12
GO:0005515	protein binding	2	12
GO:0005543	phospholipid binding	3	12
GO:0005545	1-phosphatidylinositol binding	5	12
GO:0005546	phosphatidylinositol-4,5-bisphosphate binding	4	2
GO:0008289	lipid binding	2	12
GO:0030276	clathrin binding	3	12
GO:0032050	clathrin heavy chain binding	4	2
GO:0035091	phosphatidylinositol binding	4	12
GO:0043167	ion binding	2	2
GO:0043168	anion binding	3	2
GO:1901981	phosphatidylinositol phosphate binding	5	2
GO:1902936	phosphatidylinositol bisphosphate binding	6	2

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	103	107	PF00656	0.402
CLV_C14_Caspase3-7	129	133	PF00656	0.343
CLV_C14_Caspase3-7	323	327	PF00656	0.620
CLV_NRD_NRD_1	161	163	PF00675	0.373
CLV_NRD_NRD_1	247	249	PF00675	0.352
CLV_NRD_NRD_1	254	256	PF00675	0.322
CLV_PCSK_KEX2_1	161	163	PF00082	0.337
CLV_PCSK_KEX2_1	229	231	PF00082	0.369
CLV_PCSK_KEX2_1	246	248	PF00082	0.243
CLV_PCSK_KEX2_1	254	256	PF00082	0.318
CLV_PCSK_KEX2_1	80	82	PF00082	0.243
CLV_PCSK_PC1ET2_1	229	231	PF00082	0.369
CLV_PCSK_PC1ET2_1	246	248	PF00082	0.388
CLV_PCSK_PC1ET2_1	80	82	PF00082	0.243
CLV_PCSK_PC7_1	76	82	PF00082	0.388
CLV_PCSK_SKI1_1	116	120	PF00082	0.311
CLV_PCSK_SKI1_1	221	225	PF00082	0.297
CLV_PCSK_SKI1_1	230	234	PF00082	0.218
CLV_PCSK_SKI1_1	275	279	PF00082	0.323
CLV_PCSK_SKI1_1	31	35	PF00082	0.388
DEG_APCC_DBOX_1	274	282	PF00400	0.264
DEG_Nend_UBRbox_3	1	3	PF02207	0.527
DOC_CDC14_PxL_1	278	286	PF14671	0.264
DOC_CKS1_1	422	427	PF01111	0.482
DOC_MAPK_DCC_7	456	464	PF00069	0.543
DOC_MAPK_gen_1	14	21	PF00069	0.382
DOC_MAPK_MEF2A_6	456	464	PF00069	0.543
DOC_PP1_RVXF_1	227	234	PF00149	0.280
DOC_PP2B_LxvP_1	364	367	PF13499	0.588
DOC_PP2B_LxvP_1	41	44	PF13499	0.258
DOC_PP4_FxxP_1	241	244	PF00568	0.380
DOC_PP4_FxxP_1	277	280	PF00568	0.277
DOC_PP4_FxxP_1	464	467	PF00568	0.553
DOC_USP7_MATH_1	104	108	PF00917	0.242
DOC_USP7_MATH_1	259	263	PF00917	0.450
DOC_USP7_MATH_1	357	361	PF00917	0.589
DOC_USP7_MATH_1	390	394	PF00917	0.697
DOC_USP7_UBL2_3	31	35	PF12436	0.388
DOC_USP7_UBL2_3	47	51	PF12436	0.388
DOC_WW_Pin1_4	350	355	PF00397	0.689
DOC_WW_Pin1_4	382	387	PF00397	0.641
DOC_WW_Pin1_4	421	426	PF00397	0.608
LIG_14-3-3_CanoR_1	254	263	PF00244	0.367
LIG_14-3-3_CanoR_1	81	90	PF00244	0.260
LIG_ActinCP_TwfCPI_2	464	472	PF01115	0.521
LIG_BRCT_BRCA1_1	273	277	PF00533	0.337
LIG_BRCT_BRCA1_1	397	401	PF00533	0.613
LIG_EVH1_2	460	464	PF00568	0.550
LIG_FHA_1	16	22	PF00498	0.543
LIG_FHA_1	35	41	PF00498	0.131
LIG_FHA_1	356	362	PF00498	0.448
LIG_FHA_1	370	376	PF00498	0.620
LIG_FHA_1	422	428	PF00498	0.612
LIG_FHA_2	143	149	PF00498	0.388
LIG_FHA_2	291	297	PF00498	0.466
LIG_FHA_2	302	308	PF00498	0.495
LIG_LIR_Apic_2	239	244	PF02991	0.380
LIG_LIR_Apic_2	274	280	PF02991	0.337
LIG_LIR_Gen_1	211	217	PF02991	0.313
LIG_LIR_Gen_1	236	244	PF02991	0.276
LIG_LIR_Gen_1	258	267	PF02991	0.299
LIG_LIR_Gen_1	84	95	PF02991	0.243
LIG_LIR_Gen_1	96	104	PF02991	0.243
LIG_LIR_Nem_3	167	171	PF02991	0.327
LIG_LIR_Nem_3	211	216	PF02991	0.282
LIG_LIR_Nem_3	236	241	PF02991	0.273
LIG_LIR_Nem_3	251	256	PF02991	0.374
LIG_LIR_Nem_3	258	263	PF02991	0.302
LIG_LIR_Nem_3	56	60	PF02991	0.260
LIG_LIR_Nem_3	84	90	PF02991	0.243
LIG_LIR_Nem_3	96	100	PF02991	0.243
LIG_LYPXL_S_1	447	451	PF13949	0.546
LIG_LYPXL_yS_3	448	451	PF13949	0.565
LIG_MYND_1	282	286	PF01753	0.277
LIG_Pex14_1	249	253	PF04695	0.243
LIG_SH2_CRK	213	217	PF00017	0.251
LIG_SH2_NCK_1	256	260	PF00017	0.302
LIG_SH2_STAP1	256	260	PF00017	0.302
LIG_SH2_STAT5	12	15	PF00017	0.392
LIG_SH2_STAT5	138	141	PF00017	0.257
LIG_SH2_STAT5	146	149	PF00017	0.229
LIG_SH2_STAT5	171	174	PF00017	0.279
LIG_SH2_STAT5	266	269	PF00017	0.433
LIG_SH3_2	467	472	PF14604	0.537
LIG_SH3_3	332	338	PF00018	0.551
LIG_SH3_3	351	357	PF00018	0.581
LIG_SH3_3	368	374	PF00018	0.569
LIG_SH3_3	422	428	PF00018	0.605
LIG_SH3_3	455	461	PF00018	0.686
LIG_SH3_3	464	470	PF00018	0.585
LIG_SUMO_SIM_par_1	357	363	PF11976	0.425
LIG_SUMO_SIM_par_1	87	93	PF11976	0.258
LIG_TRAF2_1	327	330	PF00917	0.538
LIG_TRAF2_1	367	370	PF00917	0.593
LIG_UBA3_1	223	229	PF00899	0.388
LIG_WRC_WIRS_1	21	26	PF05994	0.445
LIG_WRC_WIRS_1	94	99	PF05994	0.306
LIG_WW_3	333	337	PF00397	0.726
MOD_CK1_1	22	28	PF00069	0.508
MOD_CK1_1	290	296	PF00069	0.282
MOD_CK1_1	3	9	PF00069	0.560
MOD_CK2_1	104	110	PF00069	0.464
MOD_CK2_1	142	148	PF00069	0.274
MOD_CK2_1	22	28	PF00069	0.357
MOD_CK2_1	239	245	PF00069	0.393
MOD_CK2_1	290	296	PF00069	0.338
MOD_CK2_1	301	307	PF00069	0.528
MOD_CK2_1	324	330	PF00069	0.718
MOD_Cter_Amidation	159	162	PF01082	0.388
MOD_GlcNHglycan	154	157	PF01048	0.461
MOD_GlcNHglycan	24	27	PF01048	0.528
MOD_GlcNHglycan	257	260	PF01048	0.335
MOD_GlcNHglycan	412	415	PF01048	0.623
MOD_GlcNHglycan	434	437	PF01048	0.702
MOD_GlcNHglycan	452	455	PF01048	0.483
MOD_GlcNHglycan	61	64	PF01048	0.370
MOD_GlcNHglycan	8	11	PF01048	0.564
MOD_GSK3_1	104	111	PF00069	0.347
MOD_GSK3_1	15	22	PF00069	0.422
MOD_GSK3_1	255	262	PF00069	0.297
MOD_GSK3_1	266	273	PF00069	0.219
MOD_GSK3_1	316	323	PF00069	0.553
MOD_GSK3_1	343	350	PF00069	0.587
MOD_GSK3_1	365	372	PF00069	0.660
MOD_GSK3_1	376	383	PF00069	0.636
MOD_N-GLC_1	1	6	PF02516	0.610
MOD_N-GLC_1	343	348	PF02516	0.660
MOD_N-GLC_2	206	208	PF02516	0.258
MOD_N-GLC_2	476	478	PF02516	0.411
MOD_NEK2_1	108	113	PF00069	0.393
MOD_NEK2_1	119	124	PF00069	0.330
MOD_NEK2_1	270	275	PF00069	0.283
MOD_NEK2_1	34	39	PF00069	0.275
MOD_NEK2_1	389	394	PF00069	0.581
MOD_NEK2_1	450	455	PF00069	0.581
MOD_NEK2_1	474	479	PF00069	0.590
MOD_NEK2_2	259	264	PF00069	0.389
MOD_PIKK_1	365	371	PF00454	0.526
MOD_PKA_2	119	125	PF00069	0.380
MOD_PKA_2	299	305	PF00069	0.390
MOD_Plk_1	239	245	PF00069	0.284
MOD_Plk_1	320	326	PF00069	0.669
MOD_Plk_2-3	239	245	PF00069	0.286
MOD_Plk_4	104	110	PF00069	0.258
MOD_Plk_4	142	148	PF00069	0.269
MOD_Plk_4	266	272	PF00069	0.290
MOD_Plk_4	287	293	PF00069	0.277
MOD_Plk_4	90	96	PF00069	0.258
MOD_ProDKin_1	350	356	PF00069	0.685
MOD_ProDKin_1	382	388	PF00069	0.638
MOD_ProDKin_1	421	427	PF00069	0.607
MOD_SUMO_rev_2	3	9	PF00179	0.449
MOD_SUMO_rev_2	323	332	PF00179	0.702
TRG_DiLeu_BaEn_1	211	216	PF01217	0.388
TRG_ENDOCYTIC_2	115	118	PF00928	0.388
TRG_ENDOCYTIC_2	213	216	PF00928	0.285
TRG_ENDOCYTIC_2	448	451	PF00928	0.565
TRG_ER_diArg_1	161	163	PF00400	0.387
TRG_ER_diArg_1	247	249	PF00400	0.325
TRG_ER_diArg_1	253	255	PF00400	0.285
TRG_NES_CRM1_1	307	321	PF08389	0.506
TRG_Pf-PMV_PEXEL_1	285	289	PF00026	0.364

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1II26	Leptomonas seymouri	58%	100%
A0A0S4J0L1	Bodo saltans	38%	100%
A0A1X0NIY0	Trypanosomatidae	42%	97%
A0A3S5H7C6	Leishmania donovani	89%	100%
A0A422P449	Trypanosoma rangeli	45%	96%
A4HD60	Leishmania braziliensis	70%	99%
A4I0M5	Leishmania infantum	88%	100%
D0A6X3	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	41%	98%
E9AWJ2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	84%	100%
Q8VYT2	Arabidopsis thaliana	23%	80%
Q9LVD8	Arabidopsis thaliana	25%	82%
V5DSQ8	Trypanosoma cruzi	45%	97%