Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

poly A polymerase regulatory subunit, putative

Species:

Leishmania major

UniProt:

Q4QAW9_LEIMA

TriTrypDb:

LmjF.24.0110 , LMJLV39_240006400 , LMJSD75_240006100

Length:

438

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 12
NetGPI	no	yes: 0, no: 12

Cellular components
Term	Name	Level	Count
GO:0016020	membrane	2	4
GO:0031974	membrane-enclosed lumen	2	2
GO:0031981	nuclear lumen	5	2
GO:0043233	organelle lumen	3	2
GO:0070013	intracellular organelle lumen	4	2
GO:0110165	cellular anatomical entity	1	4

Expansion

Sequence features

Q4QAW9

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4QAW9

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	13
GO:0006370	7-methylguanosine mRNA capping	8	13
GO:0006396	RNA processing	6	13
GO:0006397	mRNA processing	7	13
GO:0006725	cellular aromatic compound metabolic process	3	13
GO:0006807	nitrogen compound metabolic process	2	13
GO:0008152	metabolic process	1	13
GO:0009452	7-methylguanosine RNA capping	8	13
GO:0009987	cellular process	1	13
GO:0016070	RNA metabolic process	5	13
GO:0016071	mRNA metabolic process	6	13
GO:0034641	cellular nitrogen compound metabolic process	3	13
GO:0036260	RNA capping	7	13
GO:0043170	macromolecule metabolic process	3	13
GO:0044237	cellular metabolic process	2	13
GO:0044238	primary metabolic process	2	13
GO:0046483	heterocycle metabolic process	3	13
GO:0071704	organic substance metabolic process	2	13
GO:0090304	nucleic acid metabolic process	4	13
GO:1901360	organic cyclic compound metabolic process	3	13

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	13
GO:0003746	translation elongation factor activity	4	13
GO:0003824	catalytic activity	1	13
GO:0004483	mRNA (nucleoside-2'-O-)-methyltransferase activity	6	13
GO:0005488	binding	1	13
GO:0008135	translation factor activity, RNA binding	3	13
GO:0008168	methyltransferase activity	4	13
GO:0008171	O-methyltransferase activity	5	13
GO:0008173	RNA methyltransferase activity	4	13
GO:0008174	mRNA methyltransferase activity	5	13
GO:0008757	S-adenosylmethionine-dependent methyltransferase activity	5	13
GO:0016740	transferase activity	2	13
GO:0016741	transferase activity, transferring one-carbon groups	3	13
GO:0045182	translation regulator activity	1	13
GO:0090079	translation regulator activity, nucleic acid binding	2	13
GO:0097159	organic cyclic compound binding	2	13
GO:0140098	catalytic activity, acting on RNA	3	13
GO:0140640	catalytic activity, acting on a nucleic acid	2	13
GO:1901363	heterocyclic compound binding	2	13

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	216	220	PF00656	0.395
CLV_NRD_NRD_1	134	136	PF00675	0.450
CLV_NRD_NRD_1	15	17	PF00675	0.430
CLV_NRD_NRD_1	252	254	PF00675	0.456
CLV_NRD_NRD_1	334	336	PF00675	0.513
CLV_NRD_NRD_1	39	41	PF00675	0.323
CLV_NRD_NRD_1	411	413	PF00675	0.592
CLV_NRD_NRD_1	417	419	PF00675	0.632
CLV_NRD_NRD_1	421	423	PF00675	0.625
CLV_NRD_NRD_1	426	428	PF00675	0.707
CLV_NRD_NRD_1	59	61	PF00675	0.141
CLV_NRD_NRD_1	6	8	PF00675	0.458
CLV_PCSK_FUR_1	132	136	PF00082	0.418
CLV_PCSK_KEX2_1	134	136	PF00082	0.418
CLV_PCSK_KEX2_1	15	17	PF00082	0.427
CLV_PCSK_KEX2_1	244	246	PF00082	0.557
CLV_PCSK_KEX2_1	252	254	PF00082	0.458
CLV_PCSK_KEX2_1	406	408	PF00082	0.458
CLV_PCSK_KEX2_1	411	413	PF00082	0.575
CLV_PCSK_KEX2_1	416	418	PF00082	0.570
CLV_PCSK_KEX2_1	426	428	PF00082	0.751
CLV_PCSK_KEX2_1	432	434	PF00082	0.677
CLV_PCSK_KEX2_1	58	60	PF00082	0.389
CLV_PCSK_KEX2_1	6	8	PF00082	0.450
CLV_PCSK_KEX2_1	67	69	PF00082	0.371
CLV_PCSK_PC1ET2_1	244	246	PF00082	0.512
CLV_PCSK_PC1ET2_1	406	408	PF00082	0.365
CLV_PCSK_PC1ET2_1	416	418	PF00082	0.487
CLV_PCSK_PC1ET2_1	432	434	PF00082	0.696
CLV_PCSK_PC1ET2_1	58	60	PF00082	0.430
CLV_PCSK_PC1ET2_1	67	69	PF00082	0.437
CLV_PCSK_PC7_1	402	408	PF00082	0.375
CLV_PCSK_PC7_1	412	418	PF00082	0.616
CLV_PCSK_PC7_1	422	428	PF00082	0.662
CLV_PCSK_SKI1_1	280	284	PF00082	0.390
CLV_PCSK_SKI1_1	326	330	PF00082	0.401
CLV_PCSK_SKI1_1	41	45	PF00082	0.324
CLV_PCSK_SKI1_1	6	10	PF00082	0.459
CLV_PCSK_SKI1_1	67	71	PF00082	0.331
DEG_Nend_Nbox_1	1	3	PF02207	0.539
DEG_SPOP_SBC_1	229	233	PF00917	0.336
DOC_CYCLIN_yCln2_LP_2	312	318	PF00134	0.447
DOC_CYCLIN_yCln2_LP_2	80	86	PF00134	0.425
DOC_CYCLIN_yCln2_LP_2	96	102	PF00134	0.204
DOC_MAPK_MEF2A_6	230	239	PF00069	0.330
DOC_PP2B_LxvP_1	80	83	PF13499	0.450
DOC_PP4_FxxP_1	190	193	PF00568	0.323
DOC_PP4_FxxP_1	27	30	PF00568	0.560
DOC_USP7_MATH_1	197	201	PF00917	0.471
DOC_USP7_MATH_1	229	233	PF00917	0.336
DOC_USP7_MATH_1	293	297	PF00917	0.557
DOC_WD40_RPTOR_TOS_1	179	184	PF00400	0.439
DOC_WW_Pin1_4	377	382	PF00397	0.502
LIG_14-3-3_CanoR_1	132	138	PF00244	0.370
LIG_14-3-3_CanoR_1	253	259	PF00244	0.446
LIG_14-3-3_CanoR_1	326	331	PF00244	0.376
LIG_14-3-3_CanoR_1	6	14	PF00244	0.432
LIG_14-3-3_CterR_2	433	438	PF00244	0.514
LIG_CaM_NSCaTE_8	327	334	PF13499	0.386
LIG_deltaCOP1_diTrp_1	176	179	PF00928	0.427
LIG_eIF4E_1	37	43	PF01652	0.348
LIG_FHA_1	124	130	PF00498	0.348
LIG_FHA_1	138	144	PF00498	0.292
LIG_FHA_1	162	168	PF00498	0.336
LIG_FHA_1	219	225	PF00498	0.418
LIG_FHA_2	134	140	PF00498	0.468
LIG_FHA_2	209	215	PF00498	0.452
LIG_FHA_2	255	261	PF00498	0.396
LIG_KLC1_Yacidic_2	214	219	PF13176	0.472
LIG_LIR_Apic_2	24	30	PF02991	0.548
LIG_LIR_Gen_1	213	223	PF02991	0.431
LIG_LIR_Gen_1	391	400	PF02991	0.521
LIG_LIR_Gen_1	93	102	PF02991	0.402
LIG_LIR_Nem_3	213	218	PF02991	0.431
LIG_LIR_Nem_3	309	313	PF02991	0.393
LIG_LIR_Nem_3	380	386	PF02991	0.390
LIG_LIR_Nem_3	391	395	PF02991	0.426
LIG_LIR_Nem_3	87	92	PF02991	0.411
LIG_LIR_Nem_3	93	97	PF02991	0.367
LIG_PCNA_TLS_4	280	287	PF02747	0.488
LIG_Pex14_2	383	387	PF04695	0.493
LIG_SH2_CRK	247	251	PF00017	0.519
LIG_SH2_CRK	255	259	PF00017	0.492
LIG_SH2_CRK	396	400	PF00017	0.404
LIG_SH2_GRB2like	18	21	PF00017	0.576
LIG_SH2_NCK_1	247	251	PF00017	0.546
LIG_SH2_SRC	217	220	PF00017	0.446
LIG_SH2_STAT3	205	208	PF00017	0.394
LIG_SH2_STAT5	215	218	PF00017	0.343
LIG_SH2_STAT5	255	258	PF00017	0.397
LIG_SH2_STAT5	286	289	PF00017	0.354
LIG_SH2_STAT5	342	345	PF00017	0.541
LIG_SH2_STAT5	396	399	PF00017	0.487
LIG_SH3_1	189	195	PF00018	0.336
LIG_SH3_3	11	17	PF00018	0.584
LIG_SH3_3	189	195	PF00018	0.336
LIG_SH3_3	223	229	PF00018	0.346
LIG_SH3_3	236	242	PF00018	0.313
LIG_SH3_3	312	318	PF00018	0.416
LIG_SH3_3	98	104	PF00018	0.277
LIG_SUMO_SIM_par_1	115	123	PF11976	0.355
LIG_TRAF2_1	211	214	PF00917	0.472
LIG_TRFH_1	86	90	PF08558	0.418
LIG_TYR_ITIM	340	345	PF00017	0.538
MOD_CK1_1	23	29	PF00069	0.509
MOD_CK2_1	133	139	PF00069	0.450
MOD_CK2_1	208	214	PF00069	0.445
MOD_CK2_1	228	234	PF00069	0.214
MOD_CK2_1	254	260	PF00069	0.394
MOD_CK2_1	42	48	PF00069	0.336
MOD_Cter_Amidation	420	423	PF01082	0.734
MOD_Cter_Amidation	429	432	PF01082	0.776
MOD_GlcNHglycan	150	153	PF01048	0.323
MOD_GlcNHglycan	287	290	PF01048	0.473
MOD_GlcNHglycan	61	64	PF01048	0.415
MOD_GSK3_1	108	115	PF00069	0.464
MOD_GSK3_1	133	140	PF00069	0.371
MOD_GSK3_1	144	151	PF00069	0.262
MOD_GSK3_1	298	305	PF00069	0.377
MOD_GSK3_1	326	333	PF00069	0.432
MOD_LATS_1	324	330	PF00433	0.474
MOD_N-GLC_1	20	25	PF02516	0.643
MOD_NEK2_1	218	223	PF00069	0.438
MOD_NEK2_1	330	335	PF00069	0.396
MOD_NEK2_1	84	89	PF00069	0.448
MOD_NEK2_2	254	259	PF00069	0.417
MOD_PIKK_1	218	224	PF00454	0.472
MOD_PKA_1	431	437	PF00069	0.719
MOD_PKA_1	59	65	PF00069	0.316
MOD_PKA_2	133	139	PF00069	0.376
MOD_PKA_2	59	65	PF00069	0.434
MOD_Plk_1	208	214	PF00069	0.472
MOD_Plk_1	348	354	PF00069	0.485
MOD_Plk_2-3	213	219	PF00069	0.472
MOD_Plk_4	213	219	PF00069	0.450
MOD_Plk_4	326	332	PF00069	0.394
MOD_ProDKin_1	377	383	PF00069	0.495
TRG_DiLeu_BaEn_1	46	51	PF01217	0.394
TRG_DiLeu_BaEn_4	213	219	PF01217	0.472
TRG_DiLeu_BaLyEn_6	233	238	PF01217	0.432
TRG_DiLeu_BaLyEn_6	27	32	PF01217	0.555
TRG_DiLeu_BaLyEn_6	277	282	PF01217	0.510
TRG_ENDOCYTIC_2	215	218	PF00928	0.450
TRG_ENDOCYTIC_2	255	258	PF00928	0.494
TRG_ENDOCYTIC_2	294	297	PF00928	0.432
TRG_ENDOCYTIC_2	310	313	PF00928	0.504
TRG_ENDOCYTIC_2	342	345	PF00928	0.537
TRG_ER_diArg_1	131	134	PF00400	0.450
TRG_ER_diArg_1	14	16	PF00400	0.440
TRG_ER_diArg_1	252	254	PF00400	0.484
TRG_ER_diArg_1	426	428	PF00400	0.771
TRG_ER_diArg_1	6	8	PF00400	0.453
TRG_NLS_Bipartite_1	40	62	PF00514	0.421
TRG_NLS_Bipartite_1	416	435	PF00514	0.678
TRG_Pf-PMV_PEXEL_1	280	284	PF00026	0.489
TRG_Pf-PMV_PEXEL_1	302	306	PF00026	0.463
TRG_Pf-PMV_PEXEL_1	7	11	PF00026	0.398

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1HV56	Leptomonas seymouri	68%	100%
A0A0S4J219	Bodo saltans	49%	100%
A0A1X0NIX1	Trypanosomatidae	50%	100%
A0A3S7WY48	Leishmania donovani	91%	100%
A0A422P442	Trypanosoma rangeli	52%	100%
A4HD69	Leishmania braziliensis	82%	100%
C9ZZ04	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	26%	86%
D0A6Y2	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	51%	100%
E9AH23	Leishmania infantum	92%	100%
E9AWK1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	91%	100%
V5BRC4	Trypanosoma cruzi	51%	100%