Putative ubiquitin-activating enzyme

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

ubiquitin-activating enzyme, putative

Species:

Leishmania major

UniProt:

Q4QAT5_LEIMA

TriTrypDb:

LmjF.24.0460 , LMJLV39_240010100 , LMJSD75_240009700

Length:

276

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 10
NetGPI	no	yes: 0, no: 10

Cellular components
Term	Name	Level	Count
GO:0005634	nucleus	5	2
GO:0043226	organelle	2	2
GO:0043227	membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043231	intracellular membrane-bounded organelle	4	2
GO:0110165	cellular anatomical entity	1	2

Expansion

Sequence features

Q4QAT5

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4QAT5

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	2
GO:0006396	RNA processing	6	2
GO:0006399	tRNA metabolic process	7	2
GO:0006400	tRNA modification	6	2
GO:0006725	cellular aromatic compound metabolic process	3	2
GO:0006807	nitrogen compound metabolic process	2	2
GO:0008033	tRNA processing	8	2
GO:0008152	metabolic process	1	2
GO:0009451	RNA modification	5	2
GO:0009987	cellular process	1	2
GO:0016070	RNA metabolic process	5	2
GO:0034470	ncRNA processing	7	2
GO:0034641	cellular nitrogen compound metabolic process	3	2
GO:0034660	ncRNA metabolic process	6	2
GO:0043170	macromolecule metabolic process	3	2
GO:0043412	macromolecule modification	4	2
GO:0044237	cellular metabolic process	2	2
GO:0044238	primary metabolic process	2	2
GO:0046483	heterocycle metabolic process	3	2
GO:0061504	cyclic threonylcarbamoyladenosine biosynthetic process	7	2
GO:0071704	organic substance metabolic process	2	2
GO:0090304	nucleic acid metabolic process	4	2
GO:1901360	organic cyclic compound metabolic process	3	2

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	11
GO:0008641	ubiquitin-like modifier activating enzyme activity	2	11
GO:0016829	lyase activity	2	2
GO:0016835	carbon-oxygen lyase activity	3	2
GO:0016836	hydro-lyase activity	4	2
GO:0016874	ligase activity	2	11
GO:0016877	ligase activity, forming carbon-sulfur bonds	3	11
GO:0061503	tRNA threonylcarbamoyladenosine dehydratase	5	2
GO:0140096	catalytic activity, acting on a protein	2	11
GO:0140098	catalytic activity, acting on RNA	3	2
GO:0140101	catalytic activity, acting on a tRNA	4	2
GO:0140640	catalytic activity, acting on a nucleic acid	2	2
GO:0140657	ATP-dependent activity	1	11

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	122	126	PF00656	0.266
CLV_C14_Caspase3-7	167	171	PF00656	0.299
CLV_NRD_NRD_1	180	182	PF00675	0.325
CLV_NRD_NRD_1	253	255	PF00675	0.504
CLV_NRD_NRD_1	257	259	PF00675	0.506
CLV_NRD_NRD_1	268	270	PF00675	0.543
CLV_NRD_NRD_1	85	87	PF00675	0.280
CLV_PCSK_FUR_1	253	257	PF00082	0.549
CLV_PCSK_KEX2_1	253	255	PF00082	0.454
CLV_PCSK_KEX2_1	262	264	PF00082	0.509
CLV_PCSK_KEX2_1	267	269	PF00082	0.539
CLV_PCSK_KEX2_1	85	87	PF00082	0.255
CLV_PCSK_PC1ET2_1	255	257	PF00082	0.473
CLV_PCSK_PC1ET2_1	262	264	PF00082	0.482
CLV_PCSK_PC1ET2_1	267	269	PF00082	0.459
CLV_PCSK_PC7_1	258	264	PF00082	0.486
CLV_PCSK_SKI1_1	130	134	PF00082	0.236
CLV_PCSK_SKI1_1	177	181	PF00082	0.454
CLV_PCSK_SKI1_1	262	266	PF00082	0.511
DEG_Nend_Nbox_1	1	3	PF02207	0.445
DEG_SPOP_SBC_1	58	62	PF00917	0.299
DOC_MAPK_gen_1	64	71	PF00069	0.288
DOC_MAPK_MEF2A_6	92	99	PF00069	0.222
DOC_USP7_MATH_1	144	148	PF00917	0.251
DOC_USP7_MATH_1	70	74	PF00917	0.222
DOC_USP7_UBL2_3	255	259	PF12436	0.426
DOC_USP7_UBL2_3	262	266	PF12436	0.454
DOC_WW_Pin1_4	269	274	PF00397	0.631
LIG_Actin_WH2_2	223	240	PF00022	0.373
LIG_Clathr_ClatBox_1	68	72	PF01394	0.236
LIG_FHA_1	126	132	PF00498	0.280
LIG_FHA_1	30	36	PF00498	0.355
LIG_FHA_1	8	14	PF00498	0.327
LIG_FHA_2	102	108	PF00498	0.368
LIG_FHA_2	165	171	PF00498	0.306
LIG_FHA_2	50	56	PF00498	0.222
LIG_LIR_Nem_3	55	59	PF02991	0.370
LIG_MYND_1	201	205	PF01753	0.465
LIG_SH2_CRK	56	60	PF00017	0.368
LIG_SH2_NCK_1	56	60	PF00017	0.368
LIG_SH2_STAP1	117	121	PF00017	0.222
LIG_SH3_2	202	207	PF14604	0.447
LIG_SH3_3	199	205	PF00018	0.416
LIG_SH3_4	204	211	PF00018	0.437
LIG_Sin3_3	233	240	PF02671	0.333
LIG_SUMO_SIM_anti_2	189	197	PF11976	0.368
LIG_SUMO_SIM_anti_2	50	55	PF11976	0.388
LIG_SUMO_SIM_par_1	157	163	PF11976	0.386
LIG_SUMO_SIM_par_1	67	74	PF11976	0.260
LIG_SUMO_SIM_par_1	95	100	PF11976	0.326
LIG_TYR_ITIM	54	59	PF00017	0.280
MOD_CK1_1	157	163	PF00069	0.384
MOD_CK2_1	75	81	PF00069	0.284
MOD_GlcNHglycan	233	236	PF01048	0.302
MOD_GlcNHglycan	3	6	PF01048	0.464
MOD_GSK3_1	18	25	PF00069	0.411
MOD_GSK3_1	188	195	PF00069	0.365
MOD_GSK3_1	213	220	PF00069	0.582
MOD_GSK3_1	269	276	PF00069	0.658
MOD_GSK3_1	71	78	PF00069	0.236
MOD_N-GLC_1	217	222	PF02516	0.376
MOD_NEK2_1	1	6	PF00069	0.480
MOD_NEK2_1	217	222	PF00069	0.502
MOD_NEK2_1	22	27	PF00069	0.368
MOD_NEK2_1	231	236	PF00069	0.291
MOD_NEK2_1	29	34	PF00069	0.367
MOD_NEK2_1	71	76	PF00069	0.242
MOD_OFUCOSY	123	129	PF10250	0.280
MOD_PIKK_1	7	13	PF00454	0.321
MOD_Plk_1	188	194	PF00069	0.456
MOD_Plk_1	213	219	PF00069	0.499
MOD_Plk_1	7	13	PF00069	0.307
MOD_Plk_1	71	77	PF00069	0.222
MOD_Plk_4	154	160	PF00069	0.341
MOD_Plk_4	188	194	PF00069	0.397
MOD_ProDKin_1	269	275	PF00069	0.636
MOD_SUMO_rev_2	257	264	PF00179	0.480
TRG_ENDOCYTIC_2	117	120	PF00928	0.257
TRG_ENDOCYTIC_2	56	59	PF00928	0.368
TRG_ER_diArg_1	114	117	PF00400	0.222
TRG_ER_diArg_1	253	256	PF00400	0.461
TRG_ER_diArg_1	85	88	PF00400	0.280
TRG_NLS_Bipartite_1	253	270	PF00514	0.526
TRG_NLS_MonoCore_2	265	270	PF00514	0.509
TRG_NLS_MonoExtC_3	265	270	PF00514	0.515
TRG_NLS_MonoExtN_4	263	270	PF00514	0.512
TRG_Pf-PMV_PEXEL_1	85	89	PF00026	0.280

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I6Q3	Leptomonas seymouri	85%	100%
A0A0S4KJM6	Bodo saltans	68%	84%
A0A1X0NJ23	Trypanosomatidae	66%	95%
A0A3Q8ICX5	Leishmania donovani	96%	100%
A0A422P452	Trypanosoma rangeli	68%	100%
A4I0N6	Leishmania infantum	91%	100%
D0A715	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	68%	100%
E9AWN6	Leishmania mexicana (strain MHOM/GT/2001/U1103)	90%	92%
O32037	Bacillus subtilis (strain 168)	37%	100%
Q46927	Escherichia coli (strain K12)	37%	100%
Q57097	Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)	34%	100%
V5BVX4	Trypanosoma cruzi	68%	84%