tRNA:m(4)X modification enzyme TRM13

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Methyltransferase TRM13, putative

Species:

Leishmania major

UniProt:

Q4QAS6_LEIMA

TriTrypDb:

LmjF.24.0550 , LMJLV39_240011000 , LMJSD75_240010600

Length:

513

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 12
NetGPI	no	yes: 0, no: 12

Could not find GO cellular_component term for this entry.

Expansion

Sequence features

Q4QAS6

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4QAS6

Function

Biological processes
Term	Name	Level	Count
GO:0001510	RNA methylation	4	3
GO:0006139	nucleobase-containing compound metabolic process	3	3
GO:0006396	RNA processing	6	3
GO:0006399	tRNA metabolic process	7	3
GO:0006400	tRNA modification	6	3
GO:0006725	cellular aromatic compound metabolic process	3	3
GO:0006807	nitrogen compound metabolic process	2	3
GO:0008033	tRNA processing	8	3
GO:0008152	metabolic process	1	3
GO:0009451	RNA modification	5	3
GO:0009987	cellular process	1	3
GO:0016070	RNA metabolic process	5	3
GO:0030488	tRNA methylation	5	3
GO:0032259	methylation	2	3
GO:0034470	ncRNA processing	7	3
GO:0034641	cellular nitrogen compound metabolic process	3	3
GO:0034660	ncRNA metabolic process	6	3
GO:0043170	macromolecule metabolic process	3	3
GO:0043412	macromolecule modification	4	3
GO:0043414	macromolecule methylation	3	3
GO:0044237	cellular metabolic process	2	3
GO:0044238	primary metabolic process	2	3
GO:0044260	obsolete cellular macromolecule metabolic process	3	3
GO:0046483	heterocycle metabolic process	3	3
GO:0071704	organic substance metabolic process	2	3
GO:0090304	nucleic acid metabolic process	4	3
GO:1901360	organic cyclic compound metabolic process	3	3

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	13
GO:0005488	binding	1	13
GO:0008168	methyltransferase activity	4	13
GO:0008171	O-methyltransferase activity	5	13
GO:0008173	RNA methyltransferase activity	4	13
GO:0008175	tRNA methyltransferase activity	5	13
GO:0016740	transferase activity	2	13
GO:0016741	transferase activity, transferring one-carbon groups	3	13
GO:0043167	ion binding	2	13
GO:0043169	cation binding	3	13
GO:0046872	metal ion binding	4	13
GO:0062105	RNA 2'-O-methyltransferase activity	5	13
GO:0106050	tRNA 2'-O-methyltransferase activity	6	13
GO:0140098	catalytic activity, acting on RNA	3	13
GO:0140101	catalytic activity, acting on a tRNA	4	13
GO:0140640	catalytic activity, acting on a nucleic acid	2	13

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	41	45	PF00656	0.483
CLV_C14_Caspase3-7	55	59	PF00656	0.482
CLV_C14_Caspase3-7	98	102	PF00656	0.415
CLV_NRD_NRD_1	216	218	PF00675	0.506
CLV_NRD_NRD_1	263	265	PF00675	0.599
CLV_NRD_NRD_1	28	30	PF00675	0.494
CLV_NRD_NRD_1	332	334	PF00675	0.321
CLV_NRD_NRD_1	8	10	PF00675	0.401
CLV_PCSK_FUR_1	261	265	PF00082	0.603
CLV_PCSK_FUR_1	5	9	PF00082	0.484
CLV_PCSK_KEX2_1	216	218	PF00082	0.506
CLV_PCSK_KEX2_1	263	265	PF00082	0.599
CLV_PCSK_KEX2_1	332	334	PF00082	0.322
CLV_PCSK_KEX2_1	7	9	PF00082	0.425
CLV_PCSK_PC1ET2_1	7	9	PF00082	0.395
CLV_PCSK_PC7_1	328	334	PF00082	0.349
CLV_PCSK_SKI1_1	125	129	PF00082	0.462
CLV_PCSK_SKI1_1	132	136	PF00082	0.447
CLV_PCSK_SKI1_1	193	197	PF00082	0.550
CLV_PCSK_SKI1_1	328	332	PF00082	0.404
CLV_PCSK_SKI1_1	359	363	PF00082	0.371
CLV_Separin_Metazoa	253	257	PF03568	0.505
CLV_Separin_Metazoa	374	378	PF03568	0.604
DEG_Nend_Nbox_1	1	3	PF02207	0.489
DOC_ANK_TNKS_1	39	46	PF00023	0.455
DOC_CYCLIN_RxL_1	129	138	PF00134	0.415
DOC_PP1_RVXF_1	343	350	PF00149	0.604
DOC_PP1_RVXF_1	478	484	PF00149	0.464
DOC_PP2B_LxvP_1	179	182	PF13499	0.519
DOC_PP2B_PxIxI_1	385	391	PF00149	0.571
DOC_PP4_FxxP_1	141	144	PF00568	0.424
DOC_USP7_MATH_1	153	157	PF00917	0.387
DOC_USP7_MATH_1	211	215	PF00917	0.477
DOC_USP7_MATH_1	234	238	PF00917	0.551
DOC_USP7_MATH_1	309	313	PF00917	0.541
DOC_USP7_MATH_1	474	478	PF00917	0.479
DOC_USP7_MATH_1	77	81	PF00917	0.671
DOC_WW_Pin1_4	125	130	PF00397	0.489
DOC_WW_Pin1_4	142	147	PF00397	0.480
DOC_WW_Pin1_4	499	504	PF00397	0.506
LIG_14-3-3_CanoR_1	113	123	PF00244	0.564
LIG_14-3-3_CanoR_1	132	141	PF00244	0.397
LIG_14-3-3_CanoR_1	158	164	PF00244	0.499
LIG_14-3-3_CanoR_1	207	211	PF00244	0.553
LIG_14-3-3_CanoR_1	332	338	PF00244	0.543
LIG_14-3-3_CanoR_1	348	354	PF00244	0.565
LIG_14-3-3_CanoR_1	427	435	PF00244	0.581
LIG_14-3-3_CanoR_1	447	452	PF00244	0.375
LIG_14-3-3_CanoR_1	459	465	PF00244	0.479
LIG_14-3-3_CanoR_1	9	15	PF00244	0.548
LIG_Actin_WH2_2	242	258	PF00022	0.518
LIG_AP2alpha_2	305	307	PF02296	0.506
LIG_APCC_ABBA_1	341	346	PF00400	0.583
LIG_BIR_III_2	351	355	PF00653	0.604
LIG_BRCT_BRCA1_1	16	20	PF00533	0.424
LIG_CtBP_PxDLS_1	441	447	PF00389	0.416
LIG_FHA_1	143	149	PF00498	0.520
LIG_FHA_1	152	158	PF00498	0.526
LIG_FHA_1	160	166	PF00498	0.531
LIG_FHA_1	228	234	PF00498	0.447
LIG_FHA_1	338	344	PF00498	0.583
LIG_FHA_1	358	364	PF00498	0.395
LIG_FHA_2	401	407	PF00498	0.479
LIG_FHA_2	53	59	PF00498	0.567
LIG_FHA_2	60	66	PF00498	0.562
LIG_FHA_2	98	104	PF00498	0.567
LIG_Integrin_RGD_1	42	44	PF01839	0.482
LIG_KLC1_Yacidic_2	402	406	PF13176	0.479
LIG_LIR_Apic_2	138	144	PF02991	0.445
LIG_LIR_Apic_2	402	407	PF02991	0.478
LIG_LIR_Gen_1	227	234	PF02991	0.379
LIG_LIR_Gen_1	421	428	PF02991	0.612
LIG_LIR_Gen_1	434	444	PF02991	0.395
LIG_LIR_Nem_3	227	232	PF02991	0.428
LIG_LIR_Nem_3	421	425	PF02991	0.612
LIG_LIR_Nem_3	434	439	PF02991	0.395
LIG_PCNA_TLS_4	398	405	PF02747	0.479
LIG_Pex14_1	16	20	PF04695	0.395
LIG_RPA_C_Fungi	167	179	PF08784	0.367
LIG_SH2_CRK	11	15	PF00017	0.495
LIG_SH2_CRK	436	440	PF00017	0.262
LIG_SH2_PTP2	404	407	PF00017	0.291
LIG_SH2_SRC	404	407	PF00017	0.416
LIG_SH2_STAP1	161	165	PF00017	0.419
LIG_SH2_STAP1	229	233	PF00017	0.400
LIG_SH2_STAT5	161	164	PF00017	0.422
LIG_SH2_STAT5	184	187	PF00017	0.422
LIG_SH2_STAT5	229	232	PF00017	0.434
LIG_SH2_STAT5	404	407	PF00017	0.359
LIG_SH2_STAT5	489	492	PF00017	0.371
LIG_SH2_STAT5	496	499	PF00017	0.348
LIG_SH3_2	354	359	PF14604	0.495
LIG_SH3_3	127	133	PF00018	0.626
LIG_SH3_3	147	153	PF00018	0.386
LIG_SH3_3	300	306	PF00018	0.367
LIG_SH3_3	351	357	PF00018	0.495
LIG_SH3_3	387	393	PF00018	0.367
LIG_SH3_3	459	465	PF00018	0.320
LIG_SUMO_SIM_par_1	224	231	PF11976	0.459
LIG_WRC_WIRS_1	229	234	PF05994	0.531
LIG_WRC_WIRS_1	78	83	PF05994	0.549
MOD_CDK_SPK_2	499	504	PF00069	0.359
MOD_CDK_SPxxK_3	125	132	PF00069	0.436
MOD_CDK_SPxxK_3	499	506	PF00069	0.393
MOD_CK1_1	400	406	PF00069	0.311
MOD_CK1_1	418	424	PF00069	0.183
MOD_CK1_1	454	460	PF00069	0.346
MOD_CK1_1	54	60	PF00069	0.609
MOD_CK2_1	460	466	PF00069	0.426
MOD_GlcNHglycan	135	138	PF01048	0.485
MOD_GlcNHglycan	199	202	PF01048	0.544
MOD_GlcNHglycan	369	372	PF01048	0.495
MOD_GlcNHglycan	410	413	PF01048	0.397
MOD_GlcNHglycan	417	420	PF01048	0.387
MOD_GlcNHglycan	443	447	PF01048	0.361
MOD_GlcNHglycan	449	452	PF01048	0.376
MOD_GlcNHglycan	87	90	PF01048	0.661
MOD_GSK3_1	10	17	PF00069	0.566
MOD_GSK3_1	333	340	PF00069	0.438
MOD_GSK3_1	447	454	PF00069	0.359
MOD_GSK3_1	59	66	PF00069	0.479
MOD_GSK3_1	87	94	PF00069	0.601
MOD_N-GLC_1	431	436	PF02516	0.387
MOD_N-GLC_2	50	52	PF02516	0.568
MOD_N-GLC_2	505	507	PF02516	0.466
MOD_NEK2_1	228	233	PF00069	0.531
MOD_NEK2_1	337	342	PF00069	0.495
MOD_NEK2_1	367	372	PF00069	0.367
MOD_NEK2_1	87	92	PF00069	0.488
MOD_NEK2_1	95	100	PF00069	0.463
MOD_NEK2_2	153	158	PF00069	0.387
MOD_NEK2_2	431	436	PF00069	0.444
MOD_PIKK_1	114	120	PF00454	0.424
MOD_PIKK_1	474	480	PF00454	0.320
MOD_PIKK_1	87	93	PF00454	0.493
MOD_PKA_2	206	212	PF00069	0.627
MOD_PKA_2	255	261	PF00069	0.564
MOD_PKA_2	320	326	PF00069	0.493
MOD_PKA_2	347	353	PF00069	0.433
MOD_PKA_2	426	432	PF00069	0.400
MOD_PKB_1	170	178	PF00069	0.387
MOD_PKB_1	326	334	PF00069	0.449
MOD_Plk_1	205	211	PF00069	0.499
MOD_Plk_1	234	240	PF00069	0.490
MOD_Plk_1	431	437	PF00069	0.457
MOD_Plk_4	10	16	PF00069	0.481
MOD_Plk_4	228	234	PF00069	0.414
MOD_Plk_4	298	304	PF00069	0.367
MOD_Plk_4	400	406	PF00069	0.320
MOD_Plk_4	431	437	PF00069	0.262
MOD_Plk_4	63	69	PF00069	0.591
MOD_ProDKin_1	125	131	PF00069	0.481
MOD_ProDKin_1	142	148	PF00069	0.475
MOD_ProDKin_1	499	505	PF00069	0.359
TRG_DiLeu_BaEn_1	63	68	PF01217	0.490
TRG_DiLeu_BaLyEn_6	143	148	PF01217	0.395
TRG_DiLeu_BaLyEn_6	221	226	PF01217	0.351
TRG_ENDOCYTIC_2	11	14	PF00928	0.457
TRG_ENDOCYTIC_2	229	232	PF00928	0.375
TRG_ENDOCYTIC_2	436	439	PF00928	0.262
TRG_ER_diArg_1	170	173	PF00400	0.628
TRG_ER_diArg_1	261	264	PF00400	0.571
TRG_ER_diArg_1	326	329	PF00400	0.370
TRG_ER_diArg_1	332	335	PF00400	0.367
TRG_NLS_MonoExtN_4	5	11	PF00514	0.386
TRG_Pf-PMV_PEXEL_1	105	110	PF00026	0.483
TRG_Pf-PMV_PEXEL_1	377	382	PF00026	0.449

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A1X0NJE0	Trypanosomatidae	33%	100%
A0A3Q8IG18	Leishmania donovani	52%	100%
A0A3S7WY71	Leishmania donovani	93%	100%
A4I0P4	Leishmania infantum	52%	100%
A4I0P5	Leishmania infantum	93%	100%
D0A723	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	34%	100%
E9AIR1	Leishmania braziliensis	78%	100%
E9AWP4	Leishmania mexicana (strain MHOM/GT/2001/U1103)	52%	100%
E9AWP5	Leishmania mexicana (strain MHOM/GT/2001/U1103)	89%	100%
Q4QAS7	Leishmania major	52%	100%
V5BR90	Trypanosoma cruzi	34%	97%