Zinc finger, C3HC4 type (RING finger)/EF-hand domain pair

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Zinc finger, C3HC4 type (RING finger)/EF-hand domain pair, putative

Species:

Leishmania major

UniProt:

Q4QAJ3_LEIMA

TriTrypDb:

LmjF.24.1380 , LMJLV39_240020300 , LMJSD75_240019700

Length:

394

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 26
NetGPI	no	yes: 0, no: 26

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	3
GO:0031974	membrane-enclosed lumen	2	3
GO:0031981	nuclear lumen	5	3
GO:0032838	plasma membrane bounded cell projection cytoplasm	4	3
GO:0043233	organelle lumen	3	3
GO:0070013	intracellular organelle lumen	4	3
GO:0097014	ciliary plasm	5	3
GO:0099568	cytoplasmic region	3	3
GO:0110165	cellular anatomical entity	1	3

Expansion

Sequence features

Q4QAJ3

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4QAJ3

Function

Biological processes
Term	Name	Level	Count
GO:0006807	nitrogen compound metabolic process	2	3
GO:0008152	metabolic process	1	3
GO:0016567	protein ubiquitination	7	3
GO:0019538	protein metabolic process	3	3
GO:0032446	protein modification by small protein conjugation	6	3
GO:0036211	protein modification process	4	3
GO:0043170	macromolecule metabolic process	3	3
GO:0043412	macromolecule modification	4	3
GO:0044238	primary metabolic process	2	3
GO:0070647	protein modification by small protein conjugation or removal	5	3
GO:0071704	organic substance metabolic process	2	3
GO:1901564	organonitrogen compound metabolic process	3	3

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	3
GO:0004842	ubiquitin-protein transferase activity	4	3
GO:0005488	binding	1	26
GO:0005509	calcium ion binding	5	21
GO:0016740	transferase activity	2	3
GO:0019787	ubiquitin-like protein transferase activity	3	3
GO:0043167	ion binding	2	26
GO:0043169	cation binding	3	26
GO:0046872	metal ion binding	4	26
GO:0061630	ubiquitin protein ligase activity	5	3
GO:0061659	ubiquitin-like protein ligase activity	4	3
GO:0140096	catalytic activity, acting on a protein	2	3

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	339	343	PF00656	0.449
CLV_C14_Caspase3-7	359	363	PF00656	0.310
CLV_NRD_NRD_1	76	78	PF00675	0.581
CLV_PCSK_KEX2_1	286	288	PF00082	0.445
CLV_PCSK_KEX2_1	76	78	PF00082	0.573
CLV_PCSK_PC1ET2_1	286	288	PF00082	0.445
CLV_PCSK_SKI1_1	286	290	PF00082	0.560
DOC_CYCLIN_RxL_1	271	281	PF00134	0.422
DOC_MAPK_gen_1	286	294	PF00069	0.586
DOC_MAPK_MEF2A_6	62	71	PF00069	0.454
DOC_PP1_RVXF_1	272	279	PF00149	0.443
DOC_PP4_FxxP_1	123	126	PF00568	0.574
DOC_USP7_MATH_1	128	132	PF00917	0.697
DOC_USP7_MATH_1	146	150	PF00917	0.666
DOC_USP7_MATH_1	53	57	PF00917	0.396
DOC_USP7_MATH_1	95	99	PF00917	0.660
DOC_WW_Pin1_4	103	108	PF00397	0.708
DOC_WW_Pin1_4	122	127	PF00397	0.586
DOC_WW_Pin1_4	136	141	PF00397	0.730
DOC_WW_Pin1_4	278	283	PF00397	0.562
LIG_APCC_ABBA_1	252	257	PF00400	0.448
LIG_BIR_II_1	1	5	PF00653	0.606
LIG_CaM_IQ_9	68	83	PF13499	0.326
LIG_FHA_1	13	19	PF00498	0.465
LIG_FHA_1	216	222	PF00498	0.501
LIG_FHA_2	279	285	PF00498	0.473
LIG_FHA_2	337	343	PF00498	0.509
LIG_LIR_Apic_2	21	26	PF02991	0.325
LIG_LIR_Gen_1	244	254	PF02991	0.449
LIG_LIR_Gen_1	325	334	PF02991	0.377
LIG_LIR_Gen_1	349	357	PF02991	0.323
LIG_LIR_Gen_1	385	391	PF02991	0.422
LIG_LIR_Nem_3	147	153	PF02991	0.516
LIG_LIR_Nem_3	223	228	PF02991	0.554
LIG_LIR_Nem_3	244	249	PF02991	0.482
LIG_LIR_Nem_3	251	256	PF02991	0.498
LIG_LIR_Nem_3	307	311	PF02991	0.544
LIG_LIR_Nem_3	325	330	PF02991	0.417
LIG_LIR_Nem_3	349	353	PF02991	0.328
LIG_LIR_Nem_3	385	389	PF02991	0.371
LIG_LYPXL_yS_3	150	153	PF13949	0.505
LIG_SH2_CRK	246	250	PF00017	0.437
LIG_SH2_CRK	311	315	PF00017	0.550
LIG_SH2_CRK	327	331	PF00017	0.258
LIG_SH2_GRB2like	200	203	PF00017	0.547
LIG_SH2_NCK_1	311	315	PF00017	0.432
LIG_SH2_STAP1	246	250	PF00017	0.437
LIG_SH2_STAT3	127	130	PF00017	0.697
LIG_SH2_STAT3	200	203	PF00017	0.775
LIG_SH2_STAT3	211	214	PF00017	0.663
LIG_SH3_3	224	230	PF00018	0.574
LIG_SH3_4	313	320	PF00018	0.577
LIG_SUMO_SIM_anti_2	25	31	PF11976	0.417
LIG_SUMO_SIM_par_1	217	223	PF11976	0.472
LIG_SUMO_SIM_par_1	378	385	PF11976	0.362
MOD_CK1_1	131	137	PF00069	0.818
MOD_CK1_1	191	197	PF00069	0.708
MOD_CK1_1	201	207	PF00069	0.789
MOD_CK1_1	220	226	PF00069	0.567
MOD_CK1_1	96	102	PF00069	0.575
MOD_CK2_1	298	304	PF00069	0.410
MOD_CK2_1	379	385	PF00069	0.452
MOD_GlcNHglycan	133	136	PF01048	0.764
MOD_GlcNHglycan	165	168	PF01048	0.710
MOD_GlcNHglycan	177	180	PF01048	0.649
MOD_GlcNHglycan	184	187	PF01048	0.593
MOD_GlcNHglycan	203	206	PF01048	0.523
MOD_GlcNHglycan	208	211	PF01048	0.808
MOD_GlcNHglycan	260	263	PF01048	0.495
MOD_GlcNHglycan	295	299	PF01048	0.468
MOD_GSK3_1	103	110	PF00069	0.599
MOD_GSK3_1	131	138	PF00069	0.768
MOD_GSK3_1	140	147	PF00069	0.709
MOD_GSK3_1	163	170	PF00069	0.756
MOD_GSK3_1	191	198	PF00069	0.747
MOD_GSK3_1	294	301	PF00069	0.428
MOD_GSK3_1	322	329	PF00069	0.479
MOD_GSK3_1	8	15	PF00069	0.416
MOD_N-GLC_1	198	203	PF02516	0.722
MOD_N-GLC_1	258	263	PF02516	0.436
MOD_N-GLC_1	60	65	PF02516	0.490
MOD_N-GLC_2	3	5	PF02516	0.383
MOD_NEK2_1	322	327	PF00069	0.580
MOD_NEK2_2	379	384	PF00069	0.417
MOD_OFUCOSY	92	97	PF10250	0.422
MOD_PIKK_1	109	115	PF00454	0.776
MOD_PIKK_1	140	146	PF00454	0.811
MOD_PIKK_1	157	163	PF00454	0.657
MOD_PIKK_1	188	194	PF00454	0.606
MOD_PIKK_1	320	326	PF00454	0.460
MOD_PKA_2	54	60	PF00069	0.467
MOD_PKA_2	80	86	PF00069	0.445
MOD_Plk_1	267	273	PF00069	0.412
MOD_Plk_1	348	354	PF00069	0.420
MOD_Plk_1	60	66	PF00069	0.519
MOD_Plk_2-3	8	14	PF00069	0.410
MOD_Plk_4	248	254	PF00069	0.447
MOD_Plk_4	304	310	PF00069	0.393
MOD_Plk_4	326	332	PF00069	0.324
MOD_ProDKin_1	103	109	PF00069	0.709
MOD_ProDKin_1	122	128	PF00069	0.595
MOD_ProDKin_1	136	142	PF00069	0.729
MOD_ProDKin_1	278	284	PF00069	0.558
TRG_DiLeu_BaEn_1	349	354	PF01217	0.449
TRG_ENDOCYTIC_2	150	153	PF00928	0.505
TRG_ENDOCYTIC_2	225	228	PF00928	0.475
TRG_ENDOCYTIC_2	246	249	PF00928	0.457
TRG_ENDOCYTIC_2	327	330	PF00928	0.436

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P229	Leptomonas seymouri	59%	100%
A0A0N1ILD7	Leptomonas seymouri	22%	87%
A0A0S4JBP2	Bodo saltans	24%	88%
A0A1X0NCU6	Trypanosomatidae	33%	100%
A0A3Q8IMS6	Leishmania donovani	95%	100%
A0A3R7KFH5	Trypanosoma rangeli	28%	86%
A0A3S5H5B1	Leishmania donovani	29%	67%
A0A3S7WWY0	Leishmania donovani	24%	88%
A0A3S7WYG1	Leishmania donovani	68%	100%
A4HDK3	Leishmania braziliensis	73%	100%
A4HDK4	Leishmania braziliensis	73%	100%
A4HS49	Leishmania infantum	29%	67%
A4HZP7	Leishmania infantum	24%	88%
A4I0X7	Leishmania infantum	94%	100%
A4I0X8	Leishmania infantum	67%	100%
C9ZWH1	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	29%	100%
E9AK36	Leishmania mexicana (strain MHOM/GT/2001/U1103)	29%	67%
E9AVK3	Leishmania mexicana (strain MHOM/GT/2001/U1103)	25%	88%
E9AWY1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	89%	100%
E9AWY2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	66%	100%
O97201	Leishmania major	32%	67%
Q4QAJ2	Leishmania major	71%	85%
Q4QBX6	Leishmania major	25%	88%
V5B9E6	Trypanosoma cruzi	24%	100%
V5BK04	Trypanosoma cruzi	34%	100%