Zinc finger, C3HC4 type (RING finger)/EF-hand domain pair

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Zinc finger, C3HC4 type (RING finger)/EF-hand domain pair, putative

Species:

Leishmania major

UniProt:

Q4QAJ2_LEIMA

TriTrypDb:

LmjF.24.1390 , LMJLV39_240020400 , LMJSD75_240019800

Length:

334

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 24
NetGPI	no	yes: 0, no: 24

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	3
GO:0031974	membrane-enclosed lumen	2	3
GO:0031981	nuclear lumen	5	3
GO:0032838	plasma membrane bounded cell projection cytoplasm	4	3
GO:0043233	organelle lumen	3	3
GO:0070013	intracellular organelle lumen	4	3
GO:0097014	ciliary plasm	5	3
GO:0099568	cytoplasmic region	3	3
GO:0110165	cellular anatomical entity	1	3

Expansion

Sequence features

Q4QAJ2

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4QAJ2

Function

Biological processes
Term	Name	Level	Count
GO:0006807	nitrogen compound metabolic process	2	3
GO:0008152	metabolic process	1	3
GO:0016567	protein ubiquitination	7	3
GO:0019538	protein metabolic process	3	3
GO:0032446	protein modification by small protein conjugation	6	3
GO:0036211	protein modification process	4	3
GO:0043170	macromolecule metabolic process	3	3
GO:0043412	macromolecule modification	4	3
GO:0044238	primary metabolic process	2	3
GO:0070647	protein modification by small protein conjugation or removal	5	3
GO:0071704	organic substance metabolic process	2	3
GO:1901564	organonitrogen compound metabolic process	3	3

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	3
GO:0004842	ubiquitin-protein transferase activity	4	3
GO:0005488	binding	1	24
GO:0005509	calcium ion binding	5	18
GO:0016740	transferase activity	2	3
GO:0019787	ubiquitin-like protein transferase activity	3	3
GO:0043167	ion binding	2	24
GO:0043169	cation binding	3	24
GO:0046872	metal ion binding	4	24
GO:0061630	ubiquitin protein ligase activity	5	3
GO:0061659	ubiquitin-like protein ligase activity	4	3
GO:0140096	catalytic activity, acting on a protein	2	3

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	279	283	PF00656	0.459
CLV_C14_Caspase3-7	299	303	PF00656	0.334
CLV_NRD_NRD_1	76	78	PF00675	0.594
CLV_PCSK_KEX2_1	226	228	PF00082	0.486
CLV_PCSK_KEX2_1	76	78	PF00082	0.589
CLV_PCSK_PC1ET2_1	226	228	PF00082	0.486
CLV_PCSK_SKI1_1	226	230	PF00082	0.579
DEG_SPOP_SBC_1	102	106	PF00917	0.716
DEG_SPOP_SBC_1	116	120	PF00917	0.739
DOC_CYCLIN_RxL_1	211	221	PF00134	0.476
DOC_MAPK_gen_1	226	234	PF00069	0.617
DOC_MAPK_MEF2A_6	62	71	PF00069	0.491
DOC_PP1_RVXF_1	212	219	PF00149	0.468
DOC_PP4_MxPP_1	110	113	PF00568	0.568
DOC_USP7_MATH_1	116	120	PF00917	0.705
DOC_USP7_MATH_1	129	133	PF00917	0.673
DOC_USP7_MATH_1	53	57	PF00917	0.426
DOC_USP7_MATH_1	94	98	PF00917	0.672
DOC_WW_Pin1_4	103	108	PF00397	0.739
DOC_WW_Pin1_4	158	163	PF00397	0.631
DOC_WW_Pin1_4	218	223	PF00397	0.577
LIG_14-3-3_CanoR_1	127	137	PF00244	0.453
LIG_APCC_ABBA_1	192	197	PF00400	0.463
LIG_BIR_II_1	1	5	PF00653	0.572
LIG_CaM_IQ_9	68	83	PF13499	0.340
LIG_FHA_1	13	19	PF00498	0.495
LIG_FHA_2	219	225	PF00498	0.521
LIG_FHA_2	277	283	PF00498	0.355
LIG_LIR_Apic_2	125	129	PF02991	0.513
LIG_LIR_Apic_2	21	26	PF02991	0.334
LIG_LIR_Gen_1	184	194	PF02991	0.434
LIG_LIR_Gen_1	265	274	PF02991	0.395
LIG_LIR_Gen_1	289	297	PF02991	0.353
LIG_LIR_Nem_3	136	140	PF02991	0.659
LIG_LIR_Nem_3	184	189	PF02991	0.478
LIG_LIR_Nem_3	191	196	PF02991	0.500
LIG_LIR_Nem_3	247	251	PF02991	0.522
LIG_LIR_Nem_3	265	270	PF02991	0.435
LIG_LIR_Nem_3	289	293	PF02991	0.353
LIG_LIR_Nem_3	325	329	PF02991	0.367
LIG_SH2_CRK	126	130	PF00017	0.511
LIG_SH2_CRK	140	144	PF00017	0.491
LIG_SH2_CRK	186	190	PF00017	0.433
LIG_SH2_CRK	251	255	PF00017	0.569
LIG_SH2_CRK	267	271	PF00017	0.247
LIG_SH2_NCK_1	140	144	PF00017	0.496
LIG_SH2_NCK_1	251	255	PF00017	0.475
LIG_SH2_STAP1	124	128	PF00017	0.711
LIG_SH2_STAP1	186	190	PF00017	0.426
LIG_SH2_STAT5	140	143	PF00017	0.635
LIG_SH3_3	110	116	PF00018	0.685
LIG_SH3_3	152	158	PF00018	0.761
LIG_SH3_3	159	165	PF00018	0.737
LIG_SH3_3	167	173	PF00018	0.597
LIG_SH3_3	96	102	PF00018	0.579
LIG_SH3_4	253	260	PF00018	0.602
LIG_SUMO_SIM_anti_2	25	31	PF11976	0.387
LIG_SUMO_SIM_par_1	318	325	PF11976	0.418
MOD_CK1_1	118	124	PF00069	0.728
MOD_CK2_1	238	244	PF00069	0.456
MOD_CK2_1	319	325	PF00069	0.431
MOD_GlcNHglycan	120	123	PF01048	0.717
MOD_GlcNHglycan	131	134	PF01048	0.744
MOD_GlcNHglycan	151	154	PF01048	0.756
MOD_GlcNHglycan	200	203	PF01048	0.531
MOD_GlcNHglycan	235	239	PF01048	0.465
MOD_GlcNHglycan	330	333	PF01048	0.584
MOD_GlcNHglycan	94	97	PF01048	0.581
MOD_GSK3_1	101	108	PF00069	0.624
MOD_GSK3_1	129	136	PF00069	0.719
MOD_GSK3_1	234	241	PF00069	0.472
MOD_GSK3_1	262	269	PF00069	0.512
MOD_GSK3_1	8	15	PF00069	0.429
MOD_N-GLC_1	198	203	PF02516	0.469
MOD_N-GLC_1	60	65	PF02516	0.510
MOD_NEK2_1	149	154	PF00069	0.698
MOD_NEK2_1	262	267	PF00069	0.573
MOD_NEK2_2	319	324	PF00069	0.384
MOD_PIKK_1	260	266	PF00454	0.490
MOD_PKA_2	54	60	PF00069	0.502
MOD_PKA_2	80	86	PF00069	0.385
MOD_Plk_1	207	213	PF00069	0.450
MOD_Plk_1	288	294	PF00069	0.418
MOD_Plk_1	60	66	PF00069	0.530
MOD_Plk_2-3	8	14	PF00069	0.434
MOD_Plk_4	188	194	PF00069	0.462
MOD_Plk_4	244	250	PF00069	0.427
MOD_Plk_4	266	272	PF00069	0.345
MOD_ProDKin_1	103	109	PF00069	0.741
MOD_ProDKin_1	158	164	PF00069	0.629
MOD_ProDKin_1	218	224	PF00069	0.574
TRG_DiLeu_BaEn_1	289	294	PF01217	0.464
TRG_ENDOCYTIC_2	186	189	PF00928	0.458
TRG_ENDOCYTIC_2	267	270	PF00928	0.442

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P229	Leptomonas seymouri	53%	100%
A0A0N1ILD7	Leptomonas seymouri	23%	74%
A0A0S4JBP2	Bodo saltans	25%	74%
A0A1X0NCU6	Trypanosomatidae	49%	100%
A0A1X0NKY5	Trypanosomatidae	32%	76%
A0A1X0NTS2	Trypanosomatidae	26%	75%
A0A3Q8IMS6	Leishmania donovani	70%	85%
A0A3R7KFH5	Trypanosoma rangeli	45%	73%
A0A3S7WWY0	Leishmania donovani	27%	75%
A0A3S7WYG1	Leishmania donovani	95%	100%
A4HDK3	Leishmania braziliensis	59%	85%
A4HDK4	Leishmania braziliensis	59%	85%
A4HZP7	Leishmania infantum	27%	75%
A4I0X7	Leishmania infantum	70%	85%
A4I0X8	Leishmania infantum	94%	100%
C9ZWH1	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	42%	100%
E9AVK3	Leishmania mexicana (strain MHOM/GT/2001/U1103)	26%	75%
E9AWY1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	67%	85%
E9AWY2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	91%	100%
Q4QAJ3	Leishmania major	71%	85%
Q4QBX6	Leishmania major	27%	75%
V5B9E6	Trypanosoma cruzi	33%	87%
V5BK04	Trypanosoma cruzi	48%	100%