Protein kinase domain-containing protein

Quick info Annotations Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

NPK1-related protein kinase-like

Species:

Leishmania major

UniProt:

Q4QAI6_LEIMA

TriTrypDb:

LmjF.24.1450 , LMJLV39_240021400 , LMJSD75_240020800

Length:

601

Annotations

LeishMANIAdb annotations

A large and apprently artificial collection of diverse kinetoplastid protein kinases. A subfamily has 2TM regions, but the majority is cytoplasmic.

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 2
Forrest at al. (procyclic)	no	yes: 2
Silverman et al.	no	yes: 2
Pissara et al.	no	yes: 10

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 24
NetGPI	no	yes: 0, no: 24

Cellular components
Term	Name	Level	Count
GO:0016020	membrane	2	1
GO:0110165	cellular anatomical entity	1	3
GO:0005634	nucleus	5	2
GO:0005654	nucleoplasm	2	1
GO:0005737	cytoplasm	2	1
GO:0031974	membrane-enclosed lumen	2	1
GO:0031981	nuclear lumen	5	1
GO:0032838	plasma membrane bounded cell projection cytoplasm	4	1
GO:0043226	organelle	2	2
GO:0043227	membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043231	intracellular membrane-bounded organelle	4	2
GO:0043233	organelle lumen	3	1
GO:0070013	intracellular organelle lumen	4	1
GO:0097014	ciliary plasm	5	1
GO:0099568	cytoplasmic region	3	1
GO:0016592	mediator complex	3	1
GO:0032991	protein-containing complex	1	1
GO:0140513	nuclear protein-containing complex	2	1

Expansion

Sequence features

Q4QAI6

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4QAI6

Function

Biological processes
Term	Name	Level	Count
GO:0006468	protein phosphorylation	5	25
GO:0006793	phosphorus metabolic process	3	25
GO:0006796	phosphate-containing compound metabolic process	4	25
GO:0006807	nitrogen compound metabolic process	2	25
GO:0007165	signal transduction	2	3
GO:0008152	metabolic process	1	25
GO:0009987	cellular process	1	25
GO:0016310	phosphorylation	5	25
GO:0019538	protein metabolic process	3	25
GO:0036211	protein modification process	4	25
GO:0043170	macromolecule metabolic process	3	25
GO:0043412	macromolecule modification	4	25
GO:0044237	cellular metabolic process	2	25
GO:0044238	primary metabolic process	2	25
GO:0050789	regulation of biological process	2	3
GO:0050794	regulation of cellular process	3	3
GO:0065007	biological regulation	1	3
GO:0071704	organic substance metabolic process	2	25
GO:1901564	organonitrogen compound metabolic process	3	25
GO:0035556	intracellular signal transduction	3	1

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	25
GO:0003824	catalytic activity	1	25
GO:0004672	protein kinase activity	3	25
GO:0005488	binding	1	25
GO:0005524	ATP binding	5	25
GO:0016301	kinase activity	4	25
GO:0016740	transferase activity	2	25
GO:0016772	transferase activity, transferring phosphorus-containing groups	3	25
GO:0016773	phosphotransferase activity, alcohol group as acceptor	4	25
GO:0017076	purine nucleotide binding	4	25
GO:0030554	adenyl nucleotide binding	5	25
GO:0032553	ribonucleotide binding	3	25
GO:0032555	purine ribonucleotide binding	4	25
GO:0032559	adenyl ribonucleotide binding	5	25
GO:0035639	purine ribonucleoside triphosphate binding	4	25
GO:0036094	small molecule binding	2	25
GO:0043167	ion binding	2	25
GO:0043168	anion binding	3	25
GO:0097159	organic cyclic compound binding	2	25
GO:0097367	carbohydrate derivative binding	2	25
GO:0140096	catalytic activity, acting on a protein	2	25
GO:1901265	nucleoside phosphate binding	3	25
GO:1901363	heterocyclic compound binding	2	25
GO:0004674	protein serine/threonine kinase activity	4	8
GO:0004707	MAP kinase activity	5	1
GO:0004693	cyclin-dependent protein serine/threonine kinase activity	5	1
GO:0008353	RNA polymerase II CTD heptapeptide repeat kinase activity	5	1
GO:0097472	cyclin-dependent protein kinase activity	4	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	146	150	PF00656	0.507
CLV_C14_Caspase3-7	503	507	PF00656	0.268
CLV_NRD_NRD_1	132	134	PF00675	0.550
CLV_NRD_NRD_1	161	163	PF00675	0.544
CLV_NRD_NRD_1	238	240	PF00675	0.632
CLV_NRD_NRD_1	322	324	PF00675	0.392
CLV_NRD_NRD_1	356	358	PF00675	0.273
CLV_NRD_NRD_1	37	39	PF00675	0.633
CLV_NRD_NRD_1	48	50	PF00675	0.518
CLV_NRD_NRD_1	481	483	PF00675	0.309
CLV_PCSK_KEX2_1	131	133	PF00082	0.584
CLV_PCSK_KEX2_1	160	162	PF00082	0.552
CLV_PCSK_KEX2_1	238	240	PF00082	0.634
CLV_PCSK_KEX2_1	37	39	PF00082	0.584
CLV_PCSK_KEX2_1	481	483	PF00082	0.306
CLV_PCSK_KEX2_1	583	585	PF00082	0.364
CLV_PCSK_KEX2_1	95	97	PF00082	0.546
CLV_PCSK_PC1ET2_1	131	133	PF00082	0.584
CLV_PCSK_PC1ET2_1	583	585	PF00082	0.341
CLV_PCSK_PC1ET2_1	95	97	PF00082	0.546
CLV_PCSK_PC7_1	33	39	PF00082	0.377
CLV_PCSK_PC7_1	579	585	PF00082	0.269
CLV_PCSK_SKI1_1	169	173	PF00082	0.519
CLV_PCSK_SKI1_1	419	423	PF00082	0.306
CLV_PCSK_SKI1_1	451	455	PF00082	0.486
CLV_PCSK_SKI1_1	481	485	PF00082	0.303
DEG_SPOP_SBC_1	193	197	PF00917	0.556
DOC_CKS1_1	127	132	PF01111	0.600
DOC_CKS1_1	494	499	PF01111	0.336
DOC_CYCLIN_RxL_1	80	92	PF00134	0.503
DOC_MAPK_DCC_7	80	88	PF00069	0.505
DOC_MAPK_gen_1	451	460	PF00069	0.313
DOC_PP1_RVXF_1	389	396	PF00149	0.260
DOC_PP1_RVXF_1	471	478	PF00149	0.319
DOC_PP4_FxxP_1	118	121	PF00568	0.583
DOC_USP7_MATH_1	180	184	PF00917	0.626
DOC_USP7_MATH_1	268	272	PF00917	0.569
DOC_USP7_MATH_1	281	285	PF00917	0.484
DOC_USP7_MATH_1	342	346	PF00917	0.269
DOC_USP7_MATH_1	467	471	PF00917	0.313
DOC_USP7_MATH_1	62	66	PF00917	0.582
DOC_USP7_MATH_2	121	127	PF00917	0.557
DOC_USP7_UBL2_3	50	54	PF12436	0.533
DOC_USP7_UBL2_3	90	94	PF12436	0.531
DOC_WW_Pin1_4	109	114	PF00397	0.549
DOC_WW_Pin1_4	126	131	PF00397	0.683
DOC_WW_Pin1_4	176	181	PF00397	0.522
DOC_WW_Pin1_4	257	262	PF00397	0.544
DOC_WW_Pin1_4	299	304	PF00397	0.465
DOC_WW_Pin1_4	493	498	PF00397	0.351
DOC_WW_Pin1_4	563	568	PF00397	0.298
LIG_14-3-3_CanoR_1	132	137	PF00244	0.563
LIG_14-3-3_CanoR_1	225	231	PF00244	0.547
LIG_14-3-3_CanoR_1	385	391	PF00244	0.270
LIG_14-3-3_CanoR_1	430	437	PF00244	0.276
LIG_APCC_ABBA_1	402	407	PF00400	0.281
LIG_BIR_III_4	506	510	PF00653	0.266
LIG_BRCT_BRCA1_1	26	30	PF00533	0.399
LIG_EVH1_2	226	230	PF00568	0.538
LIG_FHA_1	193	199	PF00498	0.530
LIG_FHA_1	227	233	PF00498	0.570
LIG_FHA_1	431	437	PF00498	0.288
LIG_FHA_1	482	488	PF00498	0.409
LIG_FHA_2	151	157	PF00498	0.492
LIG_FHA_2	460	466	PF00498	0.346
LIG_FHA_2	524	530	PF00498	0.297
LIG_LIR_Apic_2	117	121	PF02991	0.583
LIG_LIR_Apic_2	496	502	PF02991	0.330
LIG_LIR_Gen_1	296	306	PF02991	0.449
LIG_LIR_Gen_1	343	354	PF02991	0.295
LIG_LIR_Nem_3	296	301	PF02991	0.532
LIG_LIR_Nem_3	332	336	PF02991	0.346
LIG_LIR_Nem_3	343	349	PF02991	0.396
LIG_LIR_Nem_3	389	393	PF02991	0.367
LIG_MYND_1	218	222	PF01753	0.530
LIG_SH2_CRK	333	337	PF00017	0.375
LIG_SH2_SRC	442	445	PF00017	0.269
LIG_SH2_STAP1	295	299	PF00017	0.452
LIG_SH2_STAP1	514	518	PF00017	0.372
LIG_SH2_STAT5	295	298	PF00017	0.451
LIG_SH2_STAT5	337	340	PF00017	0.308
LIG_SH2_STAT5	394	397	PF00017	0.487
LIG_SH2_STAT5	408	411	PF00017	0.332
LIG_SH2_STAT5	442	445	PF00017	0.345
LIG_SH3_1	420	426	PF00018	0.290
LIG_SH3_2	535	540	PF14604	0.269
LIG_SH3_3	100	106	PF00018	0.560
LIG_SH3_3	215	221	PF00018	0.570
LIG_SH3_3	259	265	PF00018	0.593
LIG_SH3_3	420	426	PF00018	0.323
LIG_SH3_3	532	538	PF00018	0.272
LIG_SH3_3	55	61	PF00018	0.538
LIG_SH3_3	68	74	PF00018	0.520
LIG_SH3_4	50	57	PF00018	0.528
LIG_SUMO_SIM_anti_2	233	238	PF11976	0.528
LIG_SUMO_SIM_par_1	368	373	PF11976	0.277
LIG_SUMO_SIM_par_1	546	552	PF11976	0.206
LIG_SUMO_SIM_par_1	84	89	PF11976	0.496
LIG_TRAF2_1	197	200	PF00917	0.542
LIG_TRAF2_1	288	291	PF00917	0.483
LIG_TRAF2_1	561	564	PF00917	0.300
LIG_WRC_WIRS_1	387	392	PF05994	0.269
MOD_CDK_SPK_2	126	131	PF00069	0.591
MOD_CDK_SPxK_1	126	132	PF00069	0.587
MOD_CDK_SPxxK_3	126	133	PF00069	0.565
MOD_CK1_1	122	128	PF00069	0.600
MOD_CK1_1	135	141	PF00069	0.552
MOD_CK1_1	228	234	PF00069	0.533
MOD_CK1_1	256	262	PF00069	0.574
MOD_CK1_1	314	320	PF00069	0.409
MOD_CK1_1	488	494	PF00069	0.269
MOD_CK1_1	512	518	PF00069	0.288
MOD_CK2_1	111	117	PF00069	0.578
MOD_CK2_1	194	200	PF00069	0.585
MOD_CK2_1	285	291	PF00069	0.501
MOD_CK2_1	523	529	PF00069	0.286
MOD_GlcNHglycan	113	116	PF01048	0.514
MOD_GlcNHglycan	145	148	PF01048	0.576
MOD_GlcNHglycan	26	29	PF01048	0.400
MOD_GlcNHglycan	287	290	PF01048	0.472
MOD_GlcNHglycan	398	401	PF01048	0.424
MOD_GlcNHglycan	414	417	PF01048	0.300
MOD_GlcNHglycan	437	440	PF01048	0.350
MOD_GlcNHglycan	506	510	PF01048	0.300
MOD_GlcNHglycan	511	514	PF01048	0.302
MOD_GlcNHglycan	541	544	PF01048	0.309
MOD_GlcNHglycan	551	554	PF01048	0.315
MOD_GlcNHglycan	71	74	PF01048	0.649
MOD_GlcNHglycan	97	100	PF01048	0.562
MOD_GSK3_1	119	126	PF00069	0.547
MOD_GSK3_1	148	155	PF00069	0.559
MOD_GSK3_1	172	179	PF00069	0.541
MOD_GSK3_1	226	233	PF00069	0.587
MOD_GSK3_1	253	260	PF00069	0.531
MOD_GSK3_1	281	288	PF00069	0.630
MOD_GSK3_1	293	300	PF00069	0.435
MOD_GSK3_1	310	317	PF00069	0.379
MOD_GSK3_1	394	401	PF00069	0.212
MOD_GSK3_1	424	431	PF00069	0.242
MOD_GSK3_1	481	488	PF00069	0.329
MOD_GSK3_1	489	496	PF00069	0.387
MOD_GSK3_1	505	512	PF00069	0.299
MOD_GSK3_1	519	526	PF00069	0.449
MOD_GSK3_1	551	558	PF00069	0.342
MOD_GSK3_1	95	102	PF00069	0.567
MOD_N-GLC_1	314	319	PF02516	0.365
MOD_N-GLC_2	386	388	PF02516	0.269
MOD_NEK2_1	194	199	PF00069	0.552
MOD_NEK2_1	23	28	PF00069	0.367
MOD_NEK2_1	230	235	PF00069	0.544
MOD_NEK2_1	310	315	PF00069	0.395
MOD_NEK2_1	459	464	PF00069	0.320
MOD_NEK2_1	549	554	PF00069	0.365
MOD_NEK2_2	281	286	PF00069	0.502
MOD_NEK2_2	342	347	PF00069	0.269
MOD_PIKK_1	430	436	PF00454	0.337
MOD_PIKK_1	442	448	PF00454	0.229
MOD_PIKK_1	465	471	PF00454	0.320
MOD_PIKK_1	485	491	PF00454	0.392
MOD_PKA_1	132	138	PF00069	0.544
MOD_PKA_1	481	487	PF00069	0.269
MOD_PKA_1	583	589	PF00069	0.335
MOD_PKA_1	94	100	PF00069	0.552
MOD_PKA_2	132	138	PF00069	0.561
MOD_PKA_2	164	170	PF00069	0.627
MOD_PKA_2	285	291	PF00069	0.549
MOD_PKA_2	329	335	PF00069	0.284
MOD_PKA_2	481	487	PF00069	0.336
MOD_PKA_2	583	589	PF00069	0.326
MOD_PKA_2	95	101	PF00069	0.565
MOD_Plk_1	122	128	PF00069	0.565
MOD_Plk_1	148	154	PF00069	0.511
MOD_Plk_1	212	218	PF00069	0.562
MOD_Plk_4	332	338	PF00069	0.392
MOD_Plk_4	424	430	PF00069	0.284
MOD_Plk_4	489	495	PF00069	0.269
MOD_Plk_4	53	59	PF00069	0.535
MOD_ProDKin_1	109	115	PF00069	0.550
MOD_ProDKin_1	126	132	PF00069	0.683
MOD_ProDKin_1	176	182	PF00069	0.523
MOD_ProDKin_1	257	263	PF00069	0.545
MOD_ProDKin_1	299	305	PF00069	0.463
MOD_ProDKin_1	493	499	PF00069	0.351
MOD_ProDKin_1	563	569	PF00069	0.298
MOD_SUMO_rev_2	87	97	PF00179	0.508
TRG_ENDOCYTIC_2	333	336	PF00928	0.375
TRG_ENDOCYTIC_2	337	340	PF00928	0.375
TRG_ER_diArg_1	160	162	PF00400	0.553
TRG_ER_diArg_1	214	217	PF00400	0.558
TRG_ER_diArg_1	237	239	PF00400	0.578
TRG_ER_diArg_1	36	38	PF00400	0.347
TRG_ER_diArg_1	382	385	PF00400	0.272
TRG_ER_diArg_1	481	483	PF00400	0.334
TRG_NES_CRM1_1	362	375	PF08389	0.298
TRG_NLS_Bipartite_1	37	53	PF00514	0.489
TRG_NLS_MonoCore_2	48	53	PF00514	0.524
TRG_NLS_MonoExtC_3	418	423	PF00514	0.269
TRG_NLS_MonoExtC_3	48	53	PF00514	0.524
TRG_NLS_MonoExtN_4	130	135	PF00514	0.550
TRG_Pf-PMV_PEXEL_1	481	485	PF00026	0.275
TRG_Pf-PMV_PEXEL_1	84	89	PF00026	0.496

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1HTF0	Leptomonas seymouri	59%	95%
A0A0S4JKJ7	Bodo saltans	26%	91%
A0A3Q8ICW9	Leishmania donovani	92%	100%
A0A3Q8ITZ9	Leishmania donovani	25%	100%
A0A3S5H5G0	Leishmania donovani	26%	100%
A0A3S7WRG1	Leishmania donovani	24%	100%
A4H459	Leishmania braziliensis	26%	100%
A4H601	Leishmania braziliensis	22%	100%
A4H9U7	Leishmania braziliensis	22%	100%
A4HDJ5	Leishmania braziliensis	79%	100%
A4HFA1	Leishmania braziliensis	26%	100%
A4HLJ9	Leishmania braziliensis	24%	100%
A4HSE2	Leishmania infantum	26%	100%
A4HUS3	Leishmania infantum	24%	100%
A4I0Y4	Leishmania infantum	92%	100%
A4I910	Leishmania infantum	25%	100%
E8NHK0	Leishmania mexicana (strain MHOM/GT/2001/U1103)	23%	100%
E9ANF7	Leishmania mexicana (strain MHOM/GT/2001/U1103)	24%	100%
E9AWZ0	Leishmania mexicana (strain MHOM/GT/2001/U1103)	89%	100%
E9B3X5	Leishmania mexicana (strain MHOM/GT/2001/U1103)	25%	100%
Q4Q449	Leishmania major	25%	100%
Q4QH69	Leishmania major	24%	100%
Q4QJJ0	Leishmania major	26%	100%