VWFA domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Ssl1-like, putative

Species:

Leishmania major

UniProt:

Q4QAG3_LEIMA

TriTrypDb:

LmjF.24.1680 , LMJLV39_240024400 , LMJSD75_240023700

Length:

446

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005667	transcription regulator complex	2	2
GO:0005675	transcription factor TFIIH holo complex	4	2
GO:0032806	carboxy-terminal domain protein kinase complex	3	2
GO:0032991	protein-containing complex	1	2
GO:0061695	transferase complex, transferring phosphorus-containing groups	4	2
GO:0090575	RNA polymerase II transcription regulator complex	3	2
GO:0140513	nuclear protein-containing complex	2	2
GO:1902494	catalytic complex	2	2
GO:1902554	serine/threonine protein kinase complex	6	2
GO:1902911	protein kinase complex	5	2
GO:1990234	transferase complex	3	2
GO:0005634	nucleus	5	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1
GO:0110165	cellular anatomical entity	1	1

Expansion

Sequence features

Q4QAG3

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4QAG3

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	4
GO:0006259	DNA metabolic process	4	4
GO:0006281	DNA repair	5	4
GO:0006289	nucleotide-excision repair	6	2
GO:0006355	regulation of DNA-templated transcription	6	2
GO:0006357	regulation of transcription by RNA polymerase II	7	2
GO:0006725	cellular aromatic compound metabolic process	3	4
GO:0006807	nitrogen compound metabolic process	2	4
GO:0006950	response to stress	2	4
GO:0006974	DNA damage response	4	4
GO:0008152	metabolic process	1	4
GO:0009889	regulation of biosynthetic process	4	2
GO:0009987	cellular process	1	4
GO:0010468	regulation of gene expression	5	2
GO:0010556	regulation of macromolecule biosynthetic process	5	2
GO:0019219	regulation of nucleobase-containing compound metabolic process	5	2
GO:0019222	regulation of metabolic process	3	2
GO:0031323	regulation of cellular metabolic process	4	2
GO:0031326	regulation of cellular biosynthetic process	5	2
GO:0033554	cellular response to stress	3	4
GO:0034641	cellular nitrogen compound metabolic process	3	4
GO:0043170	macromolecule metabolic process	3	4
GO:0044237	cellular metabolic process	2	4
GO:0044238	primary metabolic process	2	4
GO:0044260	obsolete cellular macromolecule metabolic process	3	4
GO:0046483	heterocycle metabolic process	3	4
GO:0050789	regulation of biological process	2	2
GO:0050794	regulation of cellular process	3	2
GO:0050896	response to stimulus	1	4
GO:0051171	regulation of nitrogen compound metabolic process	4	2
GO:0051252	regulation of RNA metabolic process	5	2
GO:0051716	cellular response to stimulus	2	4
GO:0060255	regulation of macromolecule metabolic process	4	2
GO:0065007	biological regulation	1	2
GO:0071704	organic substance metabolic process	2	4
GO:0080090	regulation of primary metabolic process	4	2
GO:0090304	nucleic acid metabolic process	4	4
GO:1901360	organic cyclic compound metabolic process	3	4
GO:1903506	regulation of nucleic acid-templated transcription	7	2
GO:2001141	regulation of RNA biosynthetic process	6	2

Molecular functions
Term	Name	Level	Count
GO:0005488	binding	1	2
GO:0008270	zinc ion binding	6	2
GO:0043167	ion binding	2	2
GO:0043169	cation binding	3	2
GO:0046872	metal ion binding	4	2
GO:0046914	transition metal ion binding	5	2

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	248	252	PF00656	0.648
CLV_C14_Caspase3-7	9	13	PF00656	0.393
CLV_NRD_NRD_1	218	220	PF00675	0.563
CLV_NRD_NRD_1	222	224	PF00675	0.553
CLV_PCSK_FUR_1	219	223	PF00082	0.638
CLV_PCSK_FUR_1	231	235	PF00082	0.615
CLV_PCSK_KEX2_1	221	223	PF00082	0.598
CLV_PCSK_KEX2_1	233	235	PF00082	0.604
CLV_PCSK_PC1ET2_1	221	223	PF00082	0.594
CLV_PCSK_PC1ET2_1	233	235	PF00082	0.610
CLV_PCSK_PC7_1	229	235	PF00082	0.503
CLV_PCSK_SKI1_1	3	7	PF00082	0.286
CLV_PCSK_SKI1_1	308	312	PF00082	0.318
CLV_PCSK_SKI1_1	77	81	PF00082	0.285
DEG_Nend_Nbox_1	1	3	PF02207	0.343
DEG_SCF_FBW7_1	40	47	PF00400	0.393
DEG_SPOP_SBC_1	398	402	PF00917	0.447
DOC_CDC14_PxL_1	311	319	PF14671	0.416
DOC_CYCLIN_RxL_1	305	313	PF00134	0.311
DOC_CYCLIN_yCln2_LP_2	191	197	PF00134	0.265
DOC_CYCLIN_yCln2_LP_2	327	330	PF00134	0.508
DOC_PP2B_LxvP_1	267	270	PF13499	0.497
DOC_PP2B_LxvP_1	298	301	PF13499	0.414
DOC_PP2B_LxvP_1	327	330	PF13499	0.424
DOC_PP4_MxPP_1	28	31	PF00568	0.393
DOC_USP7_MATH_1	115	119	PF00917	0.621
DOC_USP7_MATH_1	13	17	PF00917	0.309
DOC_USP7_MATH_1	279	283	PF00917	0.616
DOC_USP7_MATH_1	398	402	PF00917	0.678
DOC_USP7_MATH_1	419	423	PF00917	0.516
DOC_USP7_MATH_1	44	48	PF00917	0.265
DOC_WW_Pin1_4	275	280	PF00397	0.493
DOC_WW_Pin1_4	338	343	PF00397	0.515
DOC_WW_Pin1_4	40	45	PF00397	0.343
LIG_14-3-3_CanoR_1	167	171	PF00244	0.277
LIG_14-3-3_CanoR_1	95	104	PF00244	0.255
LIG_Actin_WH2_2	18	36	PF00022	0.393
LIG_BIR_III_2	339	343	PF00653	0.500
LIG_BRCT_BRCA1_1	282	286	PF00533	0.491
LIG_EH1_1	154	162	PF00400	0.243
LIG_eIF4E_1	20	26	PF01652	0.250
LIG_FHA_1	141	147	PF00498	0.360
LIG_FHA_1	2	8	PF00498	0.289
LIG_FHA_1	377	383	PF00498	0.400
LIG_FHA_1	404	410	PF00498	0.424
LIG_FHA_1	433	439	PF00498	0.463
LIG_FHA_2	145	151	PF00498	0.282
LIG_FHA_2	355	361	PF00498	0.645
LIG_FHA_2	86	92	PF00498	0.418
LIG_LIR_Apic_2	254	260	PF02991	0.423
LIG_LIR_Gen_1	152	163	PF02991	0.312
LIG_LIR_Nem_3	152	158	PF02991	0.287
LIG_LIR_Nem_3	190	195	PF02991	0.326
LIG_MYND_1	256	260	PF01753	0.546
LIG_SH2_CRK	192	196	PF00017	0.326
LIG_SH2_SRC	257	260	PF00017	0.557
LIG_SH2_SRC	266	269	PF00017	0.473
LIG_SH2_SRC	39	42	PF00017	0.284
LIG_SH2_STAP1	197	201	PF00017	0.375
LIG_SH2_STAP1	318	322	PF00017	0.488
LIG_SH2_STAT5	192	195	PF00017	0.284
LIG_SH2_STAT5	20	23	PF00017	0.289
LIG_SH2_STAT5	24	27	PF00017	0.289
LIG_SH2_STAT5	257	260	PF00017	0.489
LIG_SH2_STAT5	266	269	PF00017	0.410
LIG_SH2_STAT5	287	290	PF00017	0.321
LIG_SH2_STAT5	39	42	PF00017	0.210
LIG_SH2_STAT5	441	444	PF00017	0.479
LIG_SH3_3	339	345	PF00018	0.505
LIG_SH3_3	392	398	PF00018	0.579
LIG_SUMO_SIM_anti_2	145	150	PF11976	0.255
LIG_SUMO_SIM_anti_2	169	175	PF11976	0.430
LIG_SUMO_SIM_anti_2	4	9	PF11976	0.295
LIG_SUMO_SIM_anti_2	70	76	PF11976	0.393
LIG_SUMO_SIM_par_1	138	143	PF11976	0.281
LIG_SUMO_SIM_par_1	3	9	PF11976	0.301
LIG_SUMO_SIM_par_1	70	76	PF11976	0.393
LIG_TRAF2_1	357	360	PF00917	0.494
LIG_TYR_ITIM	195	200	PF00017	0.393
LIG_WRC_WIRS_1	318	323	PF05994	0.420
LIG_WRC_WIRS_1	378	383	PF05994	0.353
MOD_CK1_1	169	175	PF00069	0.277
MOD_CK1_1	282	288	PF00069	0.557
MOD_CK1_1	323	329	PF00069	0.499
MOD_CK1_1	401	407	PF00069	0.647
MOD_CK1_1	85	91	PF00069	0.399
MOD_CK2_1	354	360	PF00069	0.630
MOD_CK2_1	85	91	PF00069	0.407
MOD_GlcNHglycan	132	135	PF01048	0.491
MOD_GlcNHglycan	273	276	PF01048	0.637
MOD_GlcNHglycan	312	315	PF01048	0.399
MOD_GlcNHglycan	350	353	PF01048	0.675
MOD_GlcNHglycan	401	404	PF01048	0.665
MOD_GlcNHglycan	417	420	PF01048	0.370
MOD_GlcNHglycan	84	87	PF01048	0.263
MOD_GSK3_1	111	118	PF00069	0.558
MOD_GSK3_1	140	147	PF00069	0.279
MOD_GSK3_1	162	169	PF00069	0.289
MOD_GSK3_1	271	278	PF00069	0.638
MOD_GSK3_1	296	303	PF00069	0.343
MOD_GSK3_1	316	323	PF00069	0.304
MOD_GSK3_1	348	355	PF00069	0.616
MOD_GSK3_1	397	404	PF00069	0.651
MOD_GSK3_1	40	47	PF00069	0.356
MOD_GSK3_1	415	422	PF00069	0.453
MOD_GSK3_1	82	89	PF00069	0.393
MOD_N-GLC_1	14	19	PF02516	0.250
MOD_N-GLC_1	348	353	PF02516	0.535
MOD_N-GLC_1	86	91	PF02516	0.253
MOD_N-GLC_2	426	428	PF02516	0.443
MOD_NEK2_1	1	6	PF00069	0.302
MOD_NEK2_1	142	147	PF00069	0.255
MOD_NEK2_1	310	315	PF00069	0.373
MOD_NEK2_1	316	321	PF00069	0.351
MOD_NEK2_1	376	381	PF00069	0.448
MOD_NEK2_2	162	167	PF00069	0.393
MOD_PIKK_1	132	138	PF00454	0.405
MOD_PK_1	117	123	PF00069	0.496
MOD_PKA_1	116	122	PF00069	0.667
MOD_PKA_2	166	172	PF00069	0.394
MOD_Plk_1	86	92	PF00069	0.253
MOD_Plk_2-3	181	187	PF00069	0.277
MOD_Plk_4	144	150	PF00069	0.250
MOD_Plk_4	169	175	PF00069	0.376
MOD_Plk_4	187	193	PF00069	0.156
MOD_Plk_4	317	323	PF00069	0.351
MOD_Plk_4	377	383	PF00069	0.351
MOD_Plk_4	403	409	PF00069	0.621
MOD_ProDKin_1	275	281	PF00069	0.489
MOD_ProDKin_1	338	344	PF00069	0.518
MOD_ProDKin_1	40	46	PF00069	0.343
TRG_DiLeu_BaEn_2	75	81	PF01217	0.265
TRG_DiLeu_BaLyEn_6	28	33	PF01217	0.297
TRG_DiLeu_BaLyEn_6	305	310	PF01217	0.390
TRG_DiLeu_BaLyEn_6	57	62	PF01217	0.343
TRG_ENDOCYTIC_2	192	195	PF00928	0.257
TRG_ENDOCYTIC_2	197	200	PF00928	0.241
TRG_ENDOCYTIC_2	318	321	PF00928	0.424
TRG_ER_diArg_1	222	225	PF00400	0.450
TRG_ER_diArg_1	229	232	PF00400	0.474
TRG_NLS_MonoCore_2	218	223	PF00514	0.498
TRG_NLS_MonoExtC_3	218	223	PF00514	0.402
TRG_NLS_MonoExtN_4	219	225	PF00514	0.499

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I8I5	Leptomonas seymouri	61%	98%
A0A0S4JM00	Bodo saltans	32%	100%
A0A1X0NX76	Trypanosomatidae	37%	100%
A0A3S7WYH8	Leishmania donovani	93%	100%
A0A422MXM9	Trypanosoma rangeli	40%	100%
A0JN27	Rattus norvegicus	25%	100%
A4HDM8	Leishmania braziliensis	80%	100%
C9ZWD7	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	43%	100%
E9AH75	Leishmania infantum	93%	100%
E9AX13	Leishmania mexicana (strain MHOM/GT/2001/U1103)	87%	100%
Q13888	Homo sapiens	25%	100%
Q2TBV5	Bos taurus	24%	100%
Q6P1K8	Homo sapiens	25%	100%
Q9JIB4	Mus musculus	25%	100%
V5BAZ0	Trypanosoma cruzi	41%	100%