SpoU_methylase domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

SpoU rRNA Methylase family, putative

Species:

Leishmania major

UniProt:

Q4QA96_LEIMA

TriTrypDb:

LmjF.24.2340 , LMJLV39_240031300 , LMJSD75_240030600

Length:

471

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005829	cytosol	2	2
GO:0110165	cellular anatomical entity	1	2

Expansion

Sequence features

Q4QA96

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4QA96

Function

Biological processes
Term	Name	Level	Count
GO:0001510	RNA methylation	4	2
GO:0002128	tRNA nucleoside ribose methylation	6	2
GO:0006139	nucleobase-containing compound metabolic process	3	12
GO:0006396	RNA processing	6	12
GO:0006399	tRNA metabolic process	7	2
GO:0006400	tRNA modification	6	2
GO:0006725	cellular aromatic compound metabolic process	3	12
GO:0006807	nitrogen compound metabolic process	2	12
GO:0008033	tRNA processing	8	2
GO:0008152	metabolic process	1	12
GO:0009451	RNA modification	5	2
GO:0009987	cellular process	1	12
GO:0016070	RNA metabolic process	5	12
GO:0030488	tRNA methylation	5	2
GO:0032259	methylation	2	2
GO:0034470	ncRNA processing	7	2
GO:0034641	cellular nitrogen compound metabolic process	3	12
GO:0034660	ncRNA metabolic process	6	2
GO:0043170	macromolecule metabolic process	3	12
GO:0043412	macromolecule modification	4	2
GO:0043414	macromolecule methylation	3	2
GO:0044237	cellular metabolic process	2	12
GO:0044238	primary metabolic process	2	12
GO:0044260	obsolete cellular macromolecule metabolic process	3	2
GO:0046483	heterocycle metabolic process	3	12
GO:0071704	organic substance metabolic process	2	12
GO:0090304	nucleic acid metabolic process	4	12
GO:1901360	organic cyclic compound metabolic process	3	12

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	10
GO:0003723	RNA binding	4	10
GO:0003824	catalytic activity	1	12
GO:0005488	binding	1	10
GO:0008168	methyltransferase activity	4	12
GO:0008171	O-methyltransferase activity	5	2
GO:0008173	RNA methyltransferase activity	4	12
GO:0008175	tRNA methyltransferase activity	5	2
GO:0008757	S-adenosylmethionine-dependent methyltransferase activity	5	2
GO:0016300	tRNA (uracil) methyltransferase activity	6	2
GO:0016427	tRNA (cytosine) methyltransferase activity	6	2
GO:0016740	transferase activity	2	12
GO:0016741	transferase activity, transferring one-carbon groups	3	12
GO:0052665	tRNA (uracil-2'-O-)-methyltransferase activity	7	2
GO:0052666	tRNA (cytosine-2'-O-)-methyltransferase activity	7	2
GO:0062105	RNA 2'-O-methyltransferase activity	5	2
GO:0097159	organic cyclic compound binding	2	10
GO:0106050	tRNA 2'-O-methyltransferase activity	6	2
GO:0140098	catalytic activity, acting on RNA	3	12
GO:0140101	catalytic activity, acting on a tRNA	4	2
GO:0140640	catalytic activity, acting on a nucleic acid	2	12
GO:1901363	heterocyclic compound binding	2	10

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	151	155	PF00656	0.523
CLV_NRD_NRD_1	245	247	PF00675	0.372
CLV_NRD_NRD_1	286	288	PF00675	0.419
CLV_NRD_NRD_1	290	292	PF00675	0.473
CLV_NRD_NRD_1	403	405	PF00675	0.517
CLV_PCSK_KEX2_1	10	12	PF00082	0.512
CLV_PCSK_KEX2_1	244	246	PF00082	0.375
CLV_PCSK_KEX2_1	285	287	PF00082	0.443
CLV_PCSK_KEX2_1	290	292	PF00082	0.476
CLV_PCSK_KEX2_1	402	404	PF00082	0.492
CLV_PCSK_PC1ET2_1	10	12	PF00082	0.502
CLV_PCSK_PC1ET2_1	402	404	PF00082	0.492
CLV_PCSK_PC7_1	286	292	PF00082	0.447
CLV_PCSK_SKI1_1	106	110	PF00082	0.233
CLV_PCSK_SKI1_1	2	6	PF00082	0.476
CLV_PCSK_SKI1_1	238	242	PF00082	0.394
CLV_PCSK_SKI1_1	291	295	PF00082	0.515
CLV_PCSK_SKI1_1	314	318	PF00082	0.459
CLV_PCSK_SKI1_1	407	411	PF00082	0.481
CLV_PCSK_SKI1_1	88	92	PF00082	0.274
DEG_APCC_DBOX_1	402	410	PF00400	0.477
DEG_Nend_UBRbox_1	1	4	PF02207	0.576
DOC_CDC14_PxL_1	326	334	PF14671	0.424
DOC_CYCLIN_RxL_1	235	243	PF00134	0.533
DOC_CYCLIN_RxL_1	311	321	PF00134	0.361
DOC_CYCLIN_yClb1_LxF_4	86	91	PF00134	0.474
DOC_CYCLIN_yCln2_LP_2	324	330	PF00134	0.361
DOC_MAPK_gen_1	102	109	PF00069	0.448
DOC_MAPK_gen_1	244	252	PF00069	0.344
DOC_MAPK_gen_1	402	413	PF00069	0.416
DOC_MAPK_HePTP_8	401	413	PF00069	0.466
DOC_MAPK_MEF2A_6	102	109	PF00069	0.527
DOC_MAPK_MEF2A_6	245	254	PF00069	0.332
DOC_MAPK_MEF2A_6	404	413	PF00069	0.463
DOC_MAPK_MEF2A_6	75	84	PF00069	0.535
DOC_PP1_RVXF_1	36	42	PF00149	0.446
DOC_PP1_RVXF_1	86	92	PF00149	0.474
DOC_PP2B_LxvP_1	411	414	PF13499	0.311
DOC_USP7_MATH_1	180	184	PF00917	0.519
DOC_USP7_MATH_1	343	347	PF00917	0.617
DOC_USP7_MATH_1	464	468	PF00917	0.429
DOC_WW_Pin1_4	107	112	PF00397	0.439
DOC_WW_Pin1_4	212	217	PF00397	0.729
DOC_WW_Pin1_4	3	8	PF00397	0.470
DOC_WW_Pin1_4	30	35	PF00397	0.501
DOC_WW_Pin1_4	416	421	PF00397	0.368
DOC_WW_Pin1_4	433	438	PF00397	0.381
DOC_WW_Pin1_4	74	79	PF00397	0.443
LIG_14-3-3_CanoR_1	204	212	PF00244	0.710
LIG_14-3-3_CanoR_1	244	249	PF00244	0.370
LIG_14-3-3_CanoR_1	392	401	PF00244	0.325
LIG_Actin_WH2_2	391	409	PF00022	0.473
LIG_Actin_WH2_2	449	464	PF00022	0.363
LIG_APCC_ABBA_1	254	259	PF00400	0.330
LIG_BIR_III_4	18	22	PF00653	0.450
LIG_BRCT_BRCA1_1	346	350	PF00533	0.577
LIG_BRCT_BRCA1_1	435	439	PF00533	0.368
LIG_deltaCOP1_diTrp_1	121	130	PF00928	0.474
LIG_deltaCOP1_diTrp_1	18	26	PF00928	0.377
LIG_eIF4E_1	50	56	PF01652	0.428
LIG_FHA_1	294	300	PF00498	0.400
LIG_FHA_1	34	40	PF00498	0.491
LIG_FHA_2	305	311	PF00498	0.410
LIG_FXI_DFP_1	14	18	PF00024	0.430
LIG_LIR_Gen_1	278	283	PF02991	0.341
LIG_LIR_LC3C_4	371	376	PF02991	0.413
LIG_LIR_Nem_3	278	282	PF02991	0.515
LIG_LIR_Nem_3	321	326	PF02991	0.431
LIG_LYPXL_yS_3	329	332	PF13949	0.357
LIG_LYPXL_yS_3	378	381	PF13949	0.391
LIG_PCNA_yPIPBox_3	234	246	PF02747	0.473
LIG_PTB_Apo_2	298	305	PF02174	0.440
LIG_REV1ctd_RIR_1	59	66	PF16727	0.513
LIG_RPA_C_Fungi	97	109	PF08784	0.241
LIG_SH2_CRK	165	169	PF00017	0.264
LIG_SH2_PTP2	279	282	PF00017	0.348
LIG_SH2_STAP1	248	252	PF00017	0.388
LIG_SH2_STAP1	320	324	PF00017	0.337
LIG_SH2_STAT3	161	164	PF00017	0.264
LIG_SH2_STAT3	22	25	PF00017	0.527
LIG_SH2_STAT3	248	251	PF00017	0.348
LIG_SH2_STAT5	161	164	PF00017	0.274
LIG_SH2_STAT5	279	282	PF00017	0.348
LIG_SH2_STAT5	433	436	PF00017	0.336
LIG_SH3_3	108	114	PF00018	0.298
LIG_SH3_3	324	330	PF00018	0.405
LIG_SH3_3	373	379	PF00018	0.471
LIG_SH3_3	431	437	PF00018	0.334
LIG_SH3_3	61	67	PF00018	0.339
LIG_SUMO_SIM_anti_2	278	284	PF11976	0.386
LIG_SUMO_SIM_par_1	409	416	PF11976	0.314
LIG_TRAF2_1	118	121	PF00917	0.455
LIG_TYR_ITIM	255	260	PF00017	0.367
LIG_TYR_ITIM	376	381	PF00017	0.407
LIG_UBA3_1	31	38	PF00899	0.399
LIG_UBA3_1	89	97	PF00899	0.290
MOD_CDC14_SPxK_1	215	218	PF00782	0.597
MOD_CDK_SPxK_1	212	218	PF00069	0.599
MOD_CDK_SPxxK_3	3	10	PF00069	0.435
MOD_CK1_1	167	173	PF00069	0.264
MOD_CK1_1	196	202	PF00069	0.595
MOD_CK1_1	33	39	PF00069	0.463
MOD_CK1_1	344	350	PF00069	0.516
MOD_CK1_1	393	399	PF00069	0.356
MOD_CK1_1	71	77	PF00069	0.419
MOD_GlcNHglycan	134	137	PF01048	0.389
MOD_GlcNHglycan	174	177	PF01048	0.264
MOD_GlcNHglycan	84	87	PF01048	0.326
MOD_GSK3_1	192	199	PF00069	0.505
MOD_GSK3_1	341	348	PF00069	0.526
MOD_GSK3_1	357	364	PF00069	0.575
MOD_GSK3_1	464	471	PF00069	0.585
MOD_LATS_1	66	72	PF00433	0.410
MOD_N-GLC_1	344	349	PF02516	0.632
MOD_N-GLC_1	98	103	PF02516	0.305
MOD_NEK2_1	100	105	PF00069	0.461
MOD_NEK2_1	250	255	PF00069	0.248
MOD_NEK2_1	304	309	PF00069	0.395
MOD_NEK2_1	73	78	PF00069	0.329
MOD_NEK2_1	91	96	PF00069	0.298
MOD_OFUCOSY	463	469	PF10250	0.300
MOD_PIKK_1	146	152	PF00454	0.427
MOD_PIKK_1	361	367	PF00454	0.520
MOD_PIKK_1	441	447	PF00454	0.462
MOD_PKA_1	244	250	PF00069	0.369
MOD_PKA_2	203	209	PF00069	0.664
MOD_PKA_2	219	225	PF00069	0.594
MOD_PKA_2	244	250	PF00069	0.351
MOD_Plk_1	120	126	PF00069	0.205
MOD_Plk_1	390	396	PF00069	0.391
MOD_Plk_1	465	471	PF00069	0.347
MOD_Plk_4	157	163	PF00069	0.303
MOD_Plk_4	169	175	PF00069	0.225
MOD_Plk_4	250	256	PF00069	0.294
MOD_Plk_4	346	352	PF00069	0.514
MOD_Plk_4	435	441	PF00069	0.479
MOD_ProDKin_1	107	113	PF00069	0.277
MOD_ProDKin_1	212	218	PF00069	0.727
MOD_ProDKin_1	3	9	PF00069	0.470
MOD_ProDKin_1	30	36	PF00069	0.496
MOD_ProDKin_1	416	422	PF00069	0.367
MOD_ProDKin_1	433	439	PF00069	0.387
MOD_ProDKin_1	74	80	PF00069	0.282
MOD_SUMO_rev_2	40	49	PF00179	0.258
TRG_ENDOCYTIC_2	165	168	PF00928	0.268
TRG_ENDOCYTIC_2	257	260	PF00928	0.347
TRG_ENDOCYTIC_2	279	282	PF00928	0.348
TRG_ENDOCYTIC_2	329	332	PF00928	0.375
TRG_ENDOCYTIC_2	378	381	PF00928	0.544
TRG_ER_diArg_1	244	246	PF00400	0.377
TRG_ER_diArg_1	285	287	PF00400	0.556
TRG_NLS_MonoCore_2	401	406	PF00514	0.485
TRG_NLS_MonoExtN_4	402	408	PF00514	0.488
TRG_Pf-PMV_PEXEL_1	238	242	PF00026	0.488
TRG_Pf-PMV_PEXEL_1	314	318	PF00026	0.468

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1HUK1	Leptomonas seymouri	64%	100%
A0A0S4IV97	Bodo saltans	46%	93%
A0A1X0NN27	Trypanosomatidae	50%	100%
A0A3S7WYP3	Leishmania donovani	90%	100%
A0A422NV05	Trypanosoma rangeli	50%	100%
A4I142	Leishmania infantum	91%	100%
C9ZI50	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	49%	98%
E9AIR3	Leishmania braziliensis	80%	100%
E9AX80	Leishmania mexicana (strain MHOM/GT/2001/U1103)	89%	98%
V5AZL8	Trypanosoma cruzi	50%	100%