Diphthine--ammonia ligase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

MJ0570-related uncharacterized domain containing protein, putative

Species:

Leishmania major

UniProt:

Q4QA66_LEIMA

TriTrypDb:

LmjF.25.0300 , LMJLV39_250008400 , LMJSD75_250008400

Length:

612

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 9
NetGPI	no	yes: 0, no: 9

Could not find GO cellular_component term for this entry.

Expansion

Sequence features

Q4QA66

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4QA66

Function

Biological processes
Term	Name	Level	Count
GO:0006417	regulation of translation	6	2
GO:0006448	regulation of translational elongation	7	2
GO:0006807	nitrogen compound metabolic process	2	2
GO:0008152	metabolic process	1	2
GO:0009058	biosynthetic process	2	2
GO:0009889	regulation of biosynthetic process	4	2
GO:0009987	cellular process	1	2
GO:0010468	regulation of gene expression	5	2
GO:0010556	regulation of macromolecule biosynthetic process	5	2
GO:0010608	post-transcriptional regulation of gene expression	6	2
GO:0017182	peptidyl-diphthamide metabolic process	7	2
GO:0017183	peptidyl-diphthamide biosynthetic process from peptidyl-histidine	4	2
GO:0018193	peptidyl-amino acid modification	5	2
GO:0018202	peptidyl-histidine modification	6	2
GO:0019222	regulation of metabolic process	3	2
GO:0019538	protein metabolic process	3	2
GO:0031323	regulation of cellular metabolic process	4	2
GO:0031326	regulation of cellular biosynthetic process	5	2
GO:0034248	regulation of amide metabolic process	5	2
GO:0036211	protein modification process	4	2
GO:0043170	macromolecule metabolic process	3	2
GO:0043412	macromolecule modification	4	2
GO:0044237	cellular metabolic process	2	2
GO:0044238	primary metabolic process	2	2
GO:0044249	cellular biosynthetic process	3	2
GO:0050789	regulation of biological process	2	2
GO:0050794	regulation of cellular process	3	2
GO:0051171	regulation of nitrogen compound metabolic process	4	2
GO:0051246	regulation of protein metabolic process	5	2
GO:0060255	regulation of macromolecule metabolic process	4	2
GO:0065007	biological regulation	1	2
GO:0071704	organic substance metabolic process	2	2
GO:0080090	regulation of primary metabolic process	4	2
GO:1900247	regulation of cytoplasmic translational elongation	8	2
GO:1901564	organonitrogen compound metabolic process	3	2
GO:2000112	obsolete regulation of cellular macromolecule biosynthetic process	6	2

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	10
GO:0016874	ligase activity	2	10
GO:0016879	ligase activity, forming carbon-nitrogen bonds	3	10
GO:0016880	acid-ammonia (or amide) ligase activity	4	10
GO:0017178	diphthine-ammonia ligase activity	5	10
GO:0000166	nucleotide binding	3	4
GO:0005488	binding	1	4
GO:0005524	ATP binding	5	4
GO:0017076	purine nucleotide binding	4	4
GO:0030554	adenyl nucleotide binding	5	4
GO:0032553	ribonucleotide binding	3	4
GO:0032555	purine ribonucleotide binding	4	4
GO:0032559	adenyl ribonucleotide binding	5	4
GO:0035639	purine ribonucleoside triphosphate binding	4	4
GO:0036094	small molecule binding	2	4
GO:0043167	ion binding	2	4
GO:0043168	anion binding	3	4
GO:0097159	organic cyclic compound binding	2	4
GO:0097367	carbohydrate derivative binding	2	4
GO:1901265	nucleoside phosphate binding	3	4
GO:1901363	heterocyclic compound binding	2	4

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	166	170	PF00656	0.206
CLV_C14_Caspase3-7	415	419	PF00656	0.321
CLV_C14_Caspase3-7	67	71	PF00656	0.342
CLV_NRD_NRD_1	464	466	PF00675	0.433
CLV_NRD_NRD_1	81	83	PF00675	0.279
CLV_NRD_NRD_1	86	88	PF00675	0.279
CLV_PCSK_FUR_1	84	88	PF00082	0.302
CLV_PCSK_KEX2_1	464	466	PF00082	0.433
CLV_PCSK_KEX2_1	81	83	PF00082	0.279
CLV_PCSK_KEX2_1	86	88	PF00082	0.279
CLV_PCSK_PC7_1	82	88	PF00082	0.391
CLV_PCSK_SKI1_1	115	119	PF00082	0.277
CLV_PCSK_SKI1_1	327	331	PF00082	0.606
CLV_PCSK_SKI1_1	376	380	PF00082	0.398
CLV_PCSK_SKI1_1	536	540	PF00082	0.380
DEG_APCC_DBOX_1	146	154	PF00400	0.304
DEG_APCC_DBOX_1	198	206	PF00400	0.339
DEG_Nend_UBRbox_1	1	4	PF02207	0.331
DOC_CYCLIN_RxL_1	533	544	PF00134	0.434
DOC_CYCLIN_yCln2_LP_2	225	231	PF00134	0.391
DOC_CYCLIN_yCln2_LP_2	320	326	PF00134	0.447
DOC_MAPK_DCC_7	296	305	PF00069	0.319
DOC_MAPK_gen_1	279	287	PF00069	0.470
DOC_PP2B_LxvP_1	320	323	PF13499	0.459
DOC_PP2B_LxvP_1	432	435	PF13499	0.366
DOC_PP2B_LxvP_1	76	79	PF13499	0.391
DOC_PP4_FxxP_1	385	388	PF00568	0.444
DOC_USP7_MATH_1	307	311	PF00917	0.568
DOC_USP7_MATH_1	354	358	PF00917	0.512
DOC_USP7_MATH_1	374	378	PF00917	0.505
DOC_USP7_MATH_1	388	392	PF00917	0.459
DOC_USP7_MATH_1	491	495	PF00917	0.306
DOC_USP7_MATH_1	68	72	PF00917	0.319
DOC_WW_Pin1_4	248	253	PF00397	0.411
DOC_WW_Pin1_4	347	352	PF00397	0.360
DOC_WW_Pin1_4	376	381	PF00397	0.419
DOC_WW_Pin1_4	477	482	PF00397	0.462
LIG_14-3-3_CanoR_1	262	268	PF00244	0.377
LIG_14-3-3_CanoR_1	420	427	PF00244	0.423
LIG_14-3-3_CanoR_1	474	484	PF00244	0.482
LIG_14-3-3_CanoR_1	536	541	PF00244	0.434
LIG_14-3-3_CanoR_1	599	603	PF00244	0.444
LIG_Actin_WH2_2	65	83	PF00022	0.304
LIG_BRCT_BRCA1_1	327	331	PF00533	0.479
LIG_BRCT_BRCA1_1	472	476	PF00533	0.383
LIG_FHA_1	1	7	PF00498	0.323
LIG_FHA_1	178	184	PF00498	0.274
LIG_FHA_1	220	226	PF00498	0.279
LIG_FHA_1	289	295	PF00498	0.266
LIG_FHA_1	315	321	PF00498	0.463
LIG_FHA_1	434	440	PF00498	0.391
LIG_FHA_1	470	476	PF00498	0.515
LIG_FHA_1	485	491	PF00498	0.380
LIG_FHA_1	554	560	PF00498	0.351
LIG_FHA_1	573	579	PF00498	0.370
LIG_FHA_1	593	599	PF00498	0.457
LIG_FHA_2	114	120	PF00498	0.372
LIG_FHA_2	192	198	PF00498	0.396
LIG_FHA_2	236	242	PF00498	0.302
LIG_FHA_2	413	419	PF00498	0.402
LIG_FHA_2	490	496	PF00498	0.427
LIG_LIR_Apic_2	347	351	PF02991	0.472
LIG_LIR_Gen_1	108	117	PF02991	0.333
LIG_LIR_Gen_1	152	161	PF02991	0.391
LIG_LIR_Gen_1	219	228	PF02991	0.208
LIG_LIR_Gen_1	535	545	PF02991	0.444
LIG_LIR_Gen_1	58	69	PF02991	0.280
LIG_LIR_Nem_3	108	114	PF02991	0.279
LIG_LIR_Nem_3	119	124	PF02991	0.279
LIG_LIR_Nem_3	219	223	PF02991	0.208
LIG_LIR_Nem_3	379	385	PF02991	0.348
LIG_LIR_Nem_3	535	540	PF02991	0.446
LIG_LIR_Nem_3	544	548	PF02991	0.369
LIG_LIR_Nem_3	58	64	PF02991	0.280
LIG_Pex14_2	381	385	PF04695	0.378
LIG_Pex14_2	541	545	PF04695	0.499
LIG_RPA_C_Fungi	415	427	PF08784	0.344
LIG_SH2_CRK	111	115	PF00017	0.304
LIG_SH2_CRK	348	352	PF00017	0.473
LIG_SH2_CRK	61	65	PF00017	0.391
LIG_SH2_STAP1	220	224	PF00017	0.391
LIG_SH2_STAT3	59	62	PF00017	0.279
LIG_SH2_STAT3	97	100	PF00017	0.316
LIG_SH2_STAT5	155	158	PF00017	0.391
LIG_SH2_STAT5	537	540	PF00017	0.394
LIG_SH3_3	282	288	PF00018	0.406
LIG_SH3_3	404	410	PF00018	0.359
LIG_SUMO_SIM_anti_2	238	244	PF11976	0.360
LIG_SUMO_SIM_anti_2	3	8	PF11976	0.314
LIG_SUMO_SIM_par_1	2	8	PF11976	0.321
LIG_SUMO_SIM_par_1	301	306	PF11976	0.492
LIG_SUMO_SIM_par_1	412	419	PF11976	0.365
LIG_TRAF2_1	101	104	PF00917	0.397
LIG_TRAF2_1	194	197	PF00917	0.339
MOD_CDK_SPxK_1	477	483	PF00069	0.462
MOD_CK1_1	109	115	PF00069	0.375
MOD_CK2_1	113	119	PF00069	0.267
MOD_CK2_1	191	197	PF00069	0.346
MOD_CK2_1	235	241	PF00069	0.302
MOD_CK2_1	347	353	PF00069	0.452
MOD_CK2_1	354	360	PF00069	0.379
MOD_CK2_1	383	389	PF00069	0.449
MOD_CK2_1	529	535	PF00069	0.394
MOD_CK2_1	590	596	PF00069	0.480
MOD_DYRK1A_RPxSP_1	376	380	PF00069	0.406
MOD_GlcNHglycan	128	131	PF01048	0.339
MOD_GlcNHglycan	252	255	PF01048	0.373
MOD_GlcNHglycan	305	308	PF01048	0.645
MOD_GlcNHglycan	309	312	PF01048	0.600
MOD_GlcNHglycan	327	330	PF01048	0.621
MOD_GlcNHglycan	333	336	PF01048	0.484
MOD_GlcNHglycan	356	359	PF01048	0.488
MOD_GlcNHglycan	422	425	PF01048	0.477
MOD_GlcNHglycan	477	480	PF01048	0.356
MOD_GlcNHglycan	514	519	PF01048	0.417
MOD_GlcNHglycan	592	595	PF01048	0.459
MOD_GlcNHglycan	7	10	PF01048	0.327
MOD_GSK3_1	109	116	PF00069	0.391
MOD_GSK3_1	303	310	PF00069	0.634
MOD_GSK3_1	364	371	PF00069	0.460
MOD_GSK3_1	388	395	PF00069	0.447
MOD_GSK3_1	412	419	PF00069	0.549
MOD_GSK3_1	514	521	PF00069	0.548
MOD_GSK3_1	568	575	PF00069	0.455
MOD_GSK3_1	91	98	PF00069	0.391
MOD_N-GLC_1	48	53	PF02516	0.553
MOD_N-GLC_2	363	365	PF02516	0.529
MOD_NEK2_1	113	118	PF00069	0.352
MOD_NEK2_1	177	182	PF00069	0.262
MOD_NEK2_1	208	213	PF00069	0.304
MOD_NEK2_1	235	240	PF00069	0.323
MOD_NEK2_1	294	299	PF00069	0.448
MOD_NEK2_1	303	308	PF00069	0.573
MOD_NEK2_1	331	336	PF00069	0.559
MOD_NEK2_1	412	417	PF00069	0.461
MOD_NEK2_1	475	480	PF00069	0.462
MOD_NEK2_1	541	546	PF00069	0.484
MOD_NEK2_1	60	65	PF00069	0.394
MOD_NEK2_2	392	397	PF00069	0.307
MOD_PIKK_1	458	464	PF00454	0.526
MOD_PIKK_1	569	575	PF00454	0.480
MOD_PK_1	179	185	PF00069	0.308
MOD_PKA_2	198	204	PF00069	0.391
MOD_PKA_2	416	422	PF00069	0.549
MOD_PKA_2	598	604	PF00069	0.444
MOD_Plk_1	374	380	PF00069	0.412
MOD_Plk_1	91	97	PF00069	0.391
MOD_Plk_4	10	16	PF00069	0.309
MOD_Plk_4	106	112	PF00069	0.260
MOD_Plk_4	219	225	PF00069	0.277
MOD_Plk_4	364	370	PF00069	0.435
MOD_Plk_4	536	542	PF00069	0.344
MOD_Plk_4	55	61	PF00069	0.392
MOD_Plk_4	559	565	PF00069	0.340
MOD_Plk_4	91	97	PF00069	0.376
MOD_ProDKin_1	248	254	PF00069	0.403
MOD_ProDKin_1	347	353	PF00069	0.366
MOD_ProDKin_1	376	382	PF00069	0.415
MOD_ProDKin_1	477	483	PF00069	0.462
MOD_SUMO_for_1	117	120	PF00179	0.268
TRG_DiLeu_BaLyEn_6	290	295	PF01217	0.451
TRG_ENDOCYTIC_2	111	114	PF00928	0.304
TRG_ENDOCYTIC_2	155	158	PF00928	0.391
TRG_ENDOCYTIC_2	220	223	PF00928	0.206
TRG_ENDOCYTIC_2	382	385	PF00928	0.434
TRG_ENDOCYTIC_2	537	540	PF00928	0.394
TRG_ENDOCYTIC_2	61	64	PF00928	0.391
TRG_ER_diArg_1	138	141	PF00400	0.323
TRG_ER_diArg_1	464	466	PF00400	0.433
TRG_ER_diArg_1	80	82	PF00400	0.279
TRG_ER_diArg_1	84	87	PF00400	0.279
TRG_NES_CRM1_1	162	174	PF08389	0.359
TRG_Pf-PMV_PEXEL_1	193	197	PF00026	0.391
TRG_Pf-PMV_PEXEL_1	87	92	PF00026	0.333

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PEV4	Leptomonas seymouri	60%	100%
A0A1X0P4I2	Trypanosomatidae	39%	100%
A0A3R7K7F7	Trypanosoma rangeli	41%	100%
A0A3S7WYW4	Leishmania donovani	91%	100%
A4HDX7	Leishmania braziliensis	79%	100%
A4I172	Leishmania infantum	92%	100%
D0A5F8	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	38%	100%
E9AXA6	Leishmania mexicana (strain MHOM/GT/2001/U1103)	89%	100%
Q9USQ7	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	28%	100%