PPM-type phosphatase domain-containing protein

Quick info Annotations Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

protein phosphatase 2C, putative

Species:

Leishmania major

UniProt:

Q4QA19_LEIMA

TriTrypDb:

LmjF.25.0750 , LMJLV39_250013700 , LMJSD75_250013600

Length:

406

Annotations

LeishMANIAdb annotations

This large family encompasses many diverse protein phosphatases. Some appear to have evolved transmembrane segments. Very tentatively they might regulate transmembrane receptor kinases.. The TM and non-TM groups diverged early in Eukaryota and appear to be distinct enough that they probably should not be part of the same cluster. This latter group has not expanded.

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 1
Forrest at al. (metacyclic)	no	yes: 1
Forrest at al. (procyclic)	no	yes: 2
Silverman et al.	yes	yes: 1
Pissara et al.	yes	yes: 7

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 8

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 22
NetGPI	no	yes: 0, no: 22

Cellular components
Term	Name	Level	Count
GO:0005654	nucleoplasm	2	2
GO:0005737	cytoplasm	2	3
GO:0032838	plasma membrane bounded cell projection cytoplasm	4	3
GO:0097014	ciliary plasm	5	3
GO:0099568	cytoplasmic region	3	3
GO:0110165	cellular anatomical entity	1	3
GO:0031974	membrane-enclosed lumen	2	1
GO:0031981	nuclear lumen	5	1
GO:0043233	organelle lumen	3	1
GO:0070013	intracellular organelle lumen	4	1

Expansion

Sequence features

Q4QA19

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4QA19

Function

Biological processes
Term	Name	Level	Count
GO:0006470	protein dephosphorylation	5	3
GO:0006793	phosphorus metabolic process	3	3
GO:0006796	phosphate-containing compound metabolic process	4	3
GO:0006807	nitrogen compound metabolic process	2	3
GO:0008152	metabolic process	1	3
GO:0009987	cellular process	1	3
GO:0016311	dephosphorylation	5	3
GO:0019538	protein metabolic process	3	3
GO:0035970	peptidyl-threonine dephosphorylation	6	3
GO:0036211	protein modification process	4	3
GO:0043170	macromolecule metabolic process	3	3
GO:0043412	macromolecule modification	4	3
GO:0044237	cellular metabolic process	2	3
GO:0044238	primary metabolic process	2	3
GO:0071704	organic substance metabolic process	2	3
GO:1901564	organonitrogen compound metabolic process	3	3

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	23
GO:0004721	phosphoprotein phosphatase activity	3	23
GO:0004722	protein serine/threonine phosphatase activity	4	23
GO:0005488	binding	1	12
GO:0016787	hydrolase activity	2	23
GO:0016788	hydrolase activity, acting on ester bonds	3	23
GO:0016791	phosphatase activity	5	23
GO:0042578	phosphoric ester hydrolase activity	4	23
GO:0043167	ion binding	2	12
GO:0043169	cation binding	3	12
GO:0046872	metal ion binding	4	12
GO:0140096	catalytic activity, acting on a protein	2	23
GO:0017018	myosin phosphatase activity	5	10

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	96	100	PF00656	0.398
CLV_NRD_NRD_1	301	303	PF00675	0.459
CLV_PCSK_KEX2_1	322	324	PF00082	0.408
CLV_PCSK_PC1ET2_1	322	324	PF00082	0.407
CLV_PCSK_SKI1_1	206	210	PF00082	0.490
DEG_SCF_FBW7_1	333	338	PF00400	0.499
DOC_CYCLIN_RxL_1	84	94	PF00134	0.425
DOC_MAPK_gen_1	85	93	PF00069	0.367
DOC_MAPK_HePTP_8	112	124	PF00069	0.425
DOC_MAPK_MEF2A_6	115	124	PF00069	0.409
DOC_MAPK_MEF2A_6	85	93	PF00069	0.367
DOC_PP1_RVXF_1	204	211	PF00149	0.415
DOC_PP4_FxxP_1	293	296	PF00568	0.452
DOC_USP7_MATH_1	296	300	PF00917	0.571
DOC_USP7_MATH_1	304	308	PF00917	0.504
DOC_USP7_MATH_1	69	73	PF00917	0.489
DOC_USP7_UBL2_3	115	119	PF12436	0.490
DOC_WW_Pin1_4	331	336	PF00397	0.622
LIG_14-3-3_CanoR_1	382	387	PF00244	0.694
LIG_Actin_WH2_2	312	330	PF00022	0.581
LIG_BRCT_BRCA1_1	176	180	PF00533	0.364
LIG_BRCT_BRCA1_1	211	215	PF00533	0.387
LIG_BRCT_BRCA1_1	361	365	PF00533	0.514
LIG_BRCT_BRCA1_1	46	50	PF00533	0.453
LIG_BRCT_BRCA1_2	361	367	PF00533	0.566
LIG_deltaCOP1_diTrp_1	46	54	PF00928	0.455
LIG_FHA_1	107	113	PF00498	0.385
LIG_FHA_1	284	290	PF00498	0.613
LIG_FHA_1	336	342	PF00498	0.571
LIG_FHA_2	241	247	PF00498	0.444
LIG_FHA_2	33	39	PF00498	0.426
LIG_FHA_2	332	338	PF00498	0.611
LIG_FHA_2	351	357	PF00498	0.581
LIG_LIR_Gen_1	109	116	PF02991	0.395
LIG_LIR_Gen_1	212	223	PF02991	0.408
LIG_LIR_Gen_1	321	332	PF02991	0.493
LIG_LIR_Gen_1	362	372	PF02991	0.514
LIG_LIR_Nem_3	109	114	PF02991	0.406
LIG_LIR_Nem_3	212	218	PF02991	0.408
LIG_LIR_Nem_3	321	327	PF02991	0.472
LIG_LIR_Nem_3	362	368	PF02991	0.478
LIG_LIR_Nem_3	45	51	PF02991	0.453
LIG_MLH1_MIPbox_1	361	365	PF16413	0.551
LIG_PCNA_yPIPBox_3	230	238	PF02747	0.323
LIG_PCNA_yPIPBox_3	311	320	PF02747	0.574
LIG_Pex14_1	360	364	PF04695	0.555
LIG_Pex14_2	224	228	PF04695	0.364
LIG_Pex14_2	361	365	PF04695	0.514
LIG_Pex14_2	50	54	PF04695	0.367
LIG_PTB_Apo_2	16	23	PF02174	0.417
LIG_SH2_CRK	324	328	PF00017	0.468
LIG_SH2_NCK_1	280	284	PF00017	0.571
LIG_SH2_STAP1	305	309	PF00017	0.476
LIG_SH2_STAT3	236	239	PF00017	0.357
LIG_SH2_STAT5	236	239	PF00017	0.490
LIG_SH2_STAT5	364	367	PF00017	0.536
LIG_SH2_STAT5	43	46	PF00017	0.409
LIG_SH2_STAT5	65	68	PF00017	0.339
LIG_SH3_3	136	142	PF00018	0.509
LIG_SH3_3	286	292	PF00018	0.474
LIG_SUMO_SIM_par_1	196	202	PF11976	0.372
LIG_TRAF2_1	229	232	PF00917	0.323
LIG_TRAF2_1	334	337	PF00917	0.630
MOD_CK1_1	106	112	PF00069	0.342
MOD_CK2_1	188	194	PF00069	0.530
MOD_CK2_1	250	256	PF00069	0.237
MOD_CK2_1	331	337	PF00069	0.602
MOD_CK2_1	339	345	PF00069	0.629
MOD_CK2_1	350	356	PF00069	0.592
MOD_CK2_1	69	75	PF00069	0.471
MOD_CK2_1	97	103	PF00069	0.471
MOD_GlcNHglycan	188	191	PF01048	0.437
MOD_GlcNHglycan	293	296	PF01048	0.552
MOD_GlcNHglycan	298	301	PF01048	0.565
MOD_GlcNHglycan	71	74	PF01048	0.468
MOD_GlcNHglycan	99	102	PF01048	0.391
MOD_GSK3_1	170	177	PF00069	0.339
MOD_GSK3_1	331	338	PF00069	0.631
MOD_GSK3_1	370	377	PF00069	0.676
MOD_GSK3_1	392	399	PF00069	0.645
MOD_GSK3_1	75	82	PF00069	0.466
MOD_N-GLC_1	265	270	PF02516	0.401
MOD_N-GLC_1	312	317	PF02516	0.601
MOD_NEK2_1	174	179	PF00069	0.358
MOD_NEK2_1	44	49	PF00069	0.490
MOD_NEK2_2	17	22	PF00069	0.302
MOD_PIKK_1	188	194	PF00454	0.376
MOD_PIKK_1	339	345	PF00454	0.663
MOD_PIKK_1	60	66	PF00454	0.422
MOD_PIKK_1	8	14	PF00454	0.487
MOD_PKA_2	260	266	PF00069	0.323
MOD_PKA_2	296	302	PF00069	0.445
MOD_PKA_2	32	38	PF00069	0.364
MOD_Plk_1	206	212	PF00069	0.409
MOD_Plk_1	265	271	PF00069	0.401
MOD_Plk_1	312	318	PF00069	0.571
MOD_Plk_1	339	345	PF00069	0.626
MOD_Plk_4	106	112	PF00069	0.348
MOD_Plk_4	17	23	PF00069	0.458
MOD_Plk_4	284	290	PF00069	0.537
MOD_Plk_4	39	45	PF00069	0.424
MOD_Plk_4	392	398	PF00069	0.537
MOD_ProDKin_1	331	337	PF00069	0.617
MOD_SUMO_rev_2	145	149	PF00179	0.371
MOD_SUMO_rev_2	182	187	PF00179	0.360
MOD_SUMO_rev_2	189	198	PF00179	0.340
MOD_SUMO_rev_2	345	353	PF00179	0.618
TRG_DiLeu_BaEn_4	336	342	PF01217	0.633
TRG_ENDOCYTIC_2	305	308	PF00928	0.452
TRG_ENDOCYTIC_2	324	327	PF00928	0.449
TRG_ER_diArg_1	155	158	PF00400	0.354

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P5C7	Leptomonas seymouri	32%	100%
A0A0N0P8F8	Leptomonas seymouri	30%	100%
A0A0N1I0H9	Leptomonas seymouri	30%	100%
A0A0N1IHU6	Leptomonas seymouri	86%	100%
A0A0N1PAL8	Leptomonas seymouri	46%	100%
A0A0N1PET0	Leptomonas seymouri	32%	70%
A0A0S4IY95	Bodo saltans	34%	100%
A0A0S4J0A6	Bodo saltans	66%	100%
A0A0S4J8Y7	Bodo saltans	35%	79%
A0A0S4JHB4	Bodo saltans	42%	100%
A0A0S4JPC3	Bodo saltans	31%	100%
A0A0S4KIS4	Bodo saltans	29%	89%
A0A0S4KJV9	Bodo saltans	34%	94%
A0A1X0NRG3	Trypanosomatidae	32%	100%
A0A1X0NUB7	Trypanosomatidae	33%	85%
A0A1X0NVC4	Trypanosomatidae	33%	99%
A0A1X0P2A0	Trypanosomatidae	40%	100%
A0A1X0P568	Trypanosomatidae	65%	100%
A0A1X0P991	Trypanosomatidae	34%	100%
A0A1X0PAN4	Trypanosomatidae	30%	100%
A0A3Q8IEK6	Leishmania donovani	30%	100%
A0A3Q8IFG7	Leishmania donovani	45%	100%
A0A3Q8IGS1	Leishmania donovani	32%	72%
A0A3Q8IK65	Leishmania donovani	30%	100%
A0A3Q8IL63	Leishmania donovani	29%	100%
A0A3S7WTA2	Leishmania donovani	31%	100%
A0A3S7WZ14	Leishmania donovani	99%	100%
A0A422N8D7	Trypanosoma rangeli	28%	100%
A0A422NAJ1	Trypanosoma rangeli	34%	100%
A0A422NBV9	Trypanosoma rangeli	65%	100%
A0A422NL94	Trypanosoma rangeli	32%	93%
A0A422NML6	Trypanosoma rangeli	43%	100%
A0A422NS77	Trypanosoma rangeli	32%	98%
A0A422P293	Trypanosoma rangeli	34%	100%
A0BQL0	Paramecium tetraurelia	32%	100%
A0CUB5	Paramecium tetraurelia	31%	100%
A0DSB3	Paramecium tetraurelia	30%	100%
A0DTY1	Paramecium tetraurelia	31%	100%
A3A8W2	Oryza sativa subsp. japonica	27%	100%
A4H7Y6	Leishmania braziliensis	31%	100%
A4HE10	Leishmania braziliensis	94%	100%
A4HG10	Leishmania braziliensis	29%	100%
A4HHY5	Leishmania braziliensis	44%	100%
A4HKF6	Leishmania braziliensis	31%	72%
A4HNR1	Leishmania braziliensis	31%	100%
A4HWB4	Leishmania infantum	30%	100%
A4I1B7	Leishmania infantum	98%	100%
A4I329	Leishmania infantum	30%	100%
A4I565	Leishmania infantum	45%	100%
A4I7Y4	Leishmania infantum	32%	72%
A4IA25	Leishmania infantum	29%	100%
A4ICT4	Leishmania infantum	30%	100%
A5PJZ2	Bos taurus	32%	100%
C9ZJK2	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	32%	100%
C9ZMJ7	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	31%	100%
C9ZNW4	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	32%	96%
C9ZQJ2	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	39%	100%
D0A2L9	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	33%	100%
D0A2U9	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	25%	68%
D0A5L0	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	66%	100%
E9ACV0	Leishmania major	30%	100%
E9AQ14	Leishmania mexicana (strain MHOM/GT/2001/U1103)	32%	100%
E9ASH0	Leishmania mexicana (strain MHOM/GT/2001/U1103)	31%	100%
E9AXF3	Leishmania mexicana (strain MHOM/GT/2001/U1103)	98%	100%
E9AZD9	Leishmania mexicana (strain MHOM/GT/2001/U1103)	30%	100%
E9B0G2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	45%	100%
E9B2U5	Leishmania mexicana (strain MHOM/GT/2001/U1103)	31%	72%
E9B540	Leishmania mexicana (strain MHOM/GT/2001/U1103)	29%	100%
E9B541	Leishmania mexicana (strain MHOM/GT/2001/U1103)	32%	100%
O62829	Bos taurus	31%	100%
O62830	Bos taurus	32%	84%
O64583	Arabidopsis thaliana	28%	100%
O75688	Homo sapiens	33%	85%
O80492	Arabidopsis thaliana	24%	95%
O80871	Arabidopsis thaliana	29%	100%
O81716	Arabidopsis thaliana	30%	100%
P20650	Rattus norvegicus	31%	100%
P34221	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	31%	87%
P35813	Homo sapiens	31%	100%
P35814	Oryctolagus cuniculus	31%	100%
P35815	Rattus norvegicus	31%	100%
P36982	Leishmania chagasi	98%	100%
P36993	Mus musculus	32%	100%
P38089	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	25%	100%
P39966	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	30%	88%
P40371	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	26%	100%
P49443	Mus musculus	31%	100%
P49594	Caenorhabditis elegans	27%	90%
P49596	Caenorhabditis elegans	36%	100%
P49597	Arabidopsis thaliana	30%	94%
P49599	Arabidopsis thaliana	27%	100%
Q09172	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	32%	100%
Q09173	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	30%	98%
Q0IIF0	Bos taurus	29%	100%
Q0J2L7	Oryza sativa subsp. japonica	31%	100%
Q0J2R1	Oryza sativa subsp. japonica	26%	100%
Q0JLP9	Oryza sativa subsp. japonica	28%	87%
Q19775	Caenorhabditis elegans	33%	87%
Q2PC20	Bos taurus	29%	100%
Q2QWE3	Oryza sativa subsp. japonica	29%	96%
Q3EAF9	Arabidopsis thaliana	32%	100%
Q4Q225	Leishmania major	30%	100%
Q4Q2U6	Leishmania major	29%	100%
Q4Q5B1	Leishmania major	33%	72%
Q4Q7S1	Leishmania major	45%	100%
Q4QFG7	Leishmania major	31%	100%
Q53Q11	Oryza sativa subsp. japonica	28%	100%
Q5SGD2	Homo sapiens	33%	100%
Q5SMK6	Oryza sativa subsp. japonica	27%	100%
Q653S3	Oryza sativa subsp. japonica	29%	100%
Q65XK7	Oryza sativa subsp. japonica	29%	100%
Q67UP9	Oryza sativa subsp. japonica	28%	100%
Q67UX7	Oryza sativa subsp. japonica	33%	100%
Q69QZ0	Oryza sativa subsp. japonica	30%	100%
Q69VD9	Oryza sativa subsp. japonica	29%	100%
Q6AUQ4	Oryza sativa subsp. japonica	28%	100%
Q6ETK3	Oryza sativa subsp. japonica	30%	100%
Q6ING9	Xenopus laevis	28%	100%
Q6K1U4	Oryza sativa subsp. japonica	27%	78%
Q6K5I0	Oryza sativa subsp. japonica	26%	79%
Q6L4R7	Oryza sativa subsp. japonica	28%	91%
Q6L5C4	Oryza sativa subsp. japonica	35%	83%
Q6L5H6	Oryza sativa subsp. japonica	28%	100%
Q6YTI2	Oryza sativa subsp. japonica	24%	92%
Q6ZKL8	Oryza sativa subsp. japonica	27%	76%
Q7XQU7	Oryza sativa subsp. japonica	33%	100%
Q7XU84	Oryza sativa subsp. japonica	30%	100%
Q8BHN0	Mus musculus	34%	100%
Q8BXN7	Mus musculus	30%	100%
Q8CGA0	Mus musculus	32%	90%
Q8L7I4	Arabidopsis thaliana	33%	100%
Q8N3J5	Homo sapiens	30%	100%
Q8N819	Homo sapiens	28%	94%
Q8R0F6	Mus musculus	29%	100%
Q8RX37	Arabidopsis thaliana	29%	100%
Q9CAJ0	Arabidopsis thaliana	28%	79%
Q9FYN7	Oryza sativa subsp. japonica	29%	100%
Q9H0C8	Homo sapiens	29%	100%
Q9LME4	Arabidopsis thaliana	31%	100%
Q9LMT1	Arabidopsis thaliana	26%	100%
Q9LNF4	Arabidopsis thaliana	29%	100%
Q9LNP9	Arabidopsis thaliana	28%	79%
Q9LNW3	Arabidopsis thaliana	30%	92%
Q9LUU7	Arabidopsis thaliana	33%	96%
Q9M9W9	Arabidopsis thaliana	27%	100%
Q9SD02	Arabidopsis thaliana	28%	100%
Q9SLA1	Arabidopsis thaliana	30%	100%
Q9SZ53	Arabidopsis thaliana	29%	100%
Q9XEE8	Arabidopsis thaliana	30%	100%
Q9Z1Z6	Rattus norvegicus	29%	100%
Q9ZW21	Arabidopsis thaliana	28%	100%
V5AZJ4	Trypanosoma cruzi	32%	100%
V5BA03	Trypanosoma cruzi	33%	100%
V5BCX6	Trypanosoma cruzi	32%	100%
V5BH00	Trypanosoma cruzi	43%	100%
V5BSS7	Trypanosoma cruzi	65%	100%
V5DCR6	Trypanosoma cruzi	35%	99%