Calpain family cysteine protease-like protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

calpain family cysteine protease-like protein

Species:

Leishmania major

UniProt:

Q4Q9U3_LEIMA

TriTrypDb:

LmjF.25.1480 , LMJLV39_250022200 , LMJSD75_250022200

Length:

705

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 1
Forrest at al. (metacyclic)	no	yes: 3
Forrest at al. (procyclic)	no	yes: 4
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 36

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 16

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 20

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 1, no: 93
NetGPI	no	yes: 0, no: 94

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	4
GO:0110165	cellular anatomical entity	1	6
GO:0016020	membrane	2	1
GO:0005930	axoneme	2	1
GO:0005815	microtubule organizing center	2	1
GO:0005856	cytoskeleton	5	1
GO:0020016	ciliary pocket	2	1
GO:0020038	subpellicular network	2	1
GO:0030863	cortical cytoskeleton	6	1
GO:0036064	ciliary basal body	3	1
GO:0043226	organelle	2	1
GO:0043228	non-membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043232	intracellular non-membrane-bounded organelle	4	1

Expansion

Sequence features

Q4Q9U3

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4Q9U3

Function

Biological processes
Term	Name	Level	Count
GO:0006508	proteolysis	4	95
GO:0006807	nitrogen compound metabolic process	2	95
GO:0008152	metabolic process	1	95
GO:0019538	protein metabolic process	3	95
GO:0043170	macromolecule metabolic process	3	95
GO:0044238	primary metabolic process	2	95
GO:0071704	organic substance metabolic process	2	95
GO:1901564	organonitrogen compound metabolic process	3	95

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	95
GO:0004175	endopeptidase activity	4	95
GO:0004197	cysteine-type endopeptidase activity	5	95
GO:0004198	calcium-dependent cysteine-type endopeptidase activity	6	95
GO:0008233	peptidase activity	3	95
GO:0008234	cysteine-type peptidase activity	4	95
GO:0016787	hydrolase activity	2	95
GO:0140096	catalytic activity, acting on a protein	2	95

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	181	185	PF00656	0.463
CLV_C14_Caspase3-7	546	550	PF00656	0.438
CLV_NRD_NRD_1	139	141	PF00675	0.490
CLV_NRD_NRD_1	200	202	PF00675	0.287
CLV_NRD_NRD_1	265	267	PF00675	0.228
CLV_NRD_NRD_1	361	363	PF00675	0.359
CLV_NRD_NRD_1	493	495	PF00675	0.528
CLV_NRD_NRD_1	611	613	PF00675	0.333
CLV_NRD_NRD_1	690	692	PF00675	0.463
CLV_NRD_NRD_1	76	78	PF00675	0.435
CLV_PCSK_KEX2_1	192	194	PF00082	0.277
CLV_PCSK_KEX2_1	200	202	PF00082	0.278
CLV_PCSK_KEX2_1	493	495	PF00082	0.463
CLV_PCSK_KEX2_1	611	613	PF00082	0.409
CLV_PCSK_PC1ET2_1	192	194	PF00082	0.321
CLV_PCSK_SKI1_1	154	158	PF00082	0.445
CLV_PCSK_SKI1_1	250	254	PF00082	0.251
CLV_PCSK_SKI1_1	34	38	PF00082	0.272
CLV_PCSK_SKI1_1	363	367	PF00082	0.238
CLV_PCSK_SKI1_1	611	615	PF00082	0.477
CLV_Separin_Metazoa	74	78	PF03568	0.152
DEG_MDM2_SWIB_1	474	481	PF02201	0.443
DEG_SCF_TRCP1_1	80	85	PF00400	0.156
DOC_CKS1_1	380	385	PF01111	0.454
DOC_CYCLIN_RxL_1	360	368	PF00134	0.523
DOC_MAPK_gen_1	157	167	PF00069	0.406
DOC_MAPK_gen_1	208	216	PF00069	0.561
DOC_MAPK_gen_1	371	380	PF00069	0.439
DOC_MAPK_gen_1	599	609	PF00069	0.499
DOC_MAPK_gen_1	623	630	PF00069	0.384
DOC_MAPK_MEF2A_6	208	216	PF00069	0.521
DOC_MAPK_MEF2A_6	551	559	PF00069	0.304
DOC_PP2B_LxvP_1	607	610	PF13499	0.348
DOC_USP7_MATH_1	130	134	PF00917	0.540
DOC_USP7_MATH_1	20	24	PF00917	0.357
DOC_USP7_MATH_1	381	385	PF00917	0.457
DOC_USP7_MATH_1	436	440	PF00917	0.502
DOC_USP7_MATH_1	573	577	PF00917	0.482
DOC_USP7_MATH_1	86	90	PF00917	0.357
DOC_USP7_UBL2_3	12	16	PF12436	0.451
DOC_USP7_UBL2_3	123	127	PF12436	0.457
DOC_USP7_UBL2_3	209	213	PF12436	0.349
DOC_USP7_UBL2_3	250	254	PF12436	0.549
DOC_USP7_UBL2_3	316	320	PF12436	0.451
DOC_USP7_UBL2_3	389	393	PF12436	0.353
DOC_WW_Pin1_4	218	223	PF00397	0.442
DOC_WW_Pin1_4	24	29	PF00397	0.320
DOC_WW_Pin1_4	379	384	PF00397	0.466
DOC_WW_Pin1_4	448	453	PF00397	0.474
DOC_WW_Pin1_4	662	667	PF00397	0.359
LIG_14-3-3_CanoR_1	592	597	PF00244	0.405
LIG_14-3-3_CanoR_1	611	621	PF00244	0.490
LIG_Actin_WH2_2	373	391	PF00022	0.524
LIG_APCC_ABBAyCdc20_2	162	168	PF00400	0.351
LIG_BIR_III_2	10	14	PF00653	0.627
LIG_BRCT_BRCA1_1	481	485	PF00533	0.460
LIG_BRCT_BRCA1_1	488	492	PF00533	0.452
LIG_CNOT1_NIM_1	498	507	PF04054	0.208
LIG_deltaCOP1_diTrp_1	308	313	PF00928	0.523
LIG_deltaCOP1_diTrp_1	42	46	PF00928	0.224
LIG_deltaCOP1_diTrp_1	442	447	PF00928	0.447
LIG_deltaCOP1_diTrp_1	477	485	PF00928	0.458
LIG_FHA_1	281	287	PF00498	0.461
LIG_FHA_1	290	296	PF00498	0.465
LIG_FHA_1	515	521	PF00498	0.305
LIG_FHA_1	582	588	PF00498	0.488
LIG_FHA_1	71	77	PF00498	0.407
LIG_FHA_1	83	89	PF00498	0.316
LIG_FHA_1	99	105	PF00498	0.329
LIG_FHA_2	115	121	PF00498	0.437
LIG_FHA_2	355	361	PF00498	0.453
LIG_IRF3_LxIS_1	273	278	PF10401	0.538
LIG_LIR_Apic_2	196	202	PF02991	0.447
LIG_LIR_Gen_1	308	318	PF02991	0.446
LIG_LIR_Gen_1	324	333	PF02991	0.456
LIG_LIR_Gen_1	509	518	PF02991	0.369
LIG_LIR_Gen_1	549	559	PF02991	0.396
LIG_LIR_Gen_1	700	705	PF02991	0.610
LIG_LIR_Nem_3	278	284	PF02991	0.458
LIG_LIR_Nem_3	308	313	PF02991	0.450
LIG_LIR_Nem_3	315	321	PF02991	0.451
LIG_LIR_Nem_3	324	328	PF02991	0.447
LIG_LIR_Nem_3	341	346	PF02991	0.477
LIG_LIR_Nem_3	42	47	PF02991	0.282
LIG_LIR_Nem_3	451	457	PF02991	0.420
LIG_LIR_Nem_3	477	481	PF02991	0.431
LIG_LIR_Nem_3	500	504	PF02991	0.387
LIG_LIR_Nem_3	509	513	PF02991	0.338
LIG_LIR_Nem_3	526	532	PF02991	0.402
LIG_LIR_Nem_3	549	555	PF02991	0.387
LIG_LIR_Nem_3	595	600	PF02991	0.328
LIG_LIR_Nem_3	700	704	PF02991	0.541
LIG_LYPXL_yS_3	457	460	PF13949	0.411
LIG_MLH1_MIPbox_1	481	485	PF16413	0.503
LIG_PCNA_yPIPBox_3	267	281	PF02747	0.434
LIG_Pex14_1	372	376	PF04695	0.339
LIG_Pex14_1	400	404	PF04695	0.339
LIG_Pex14_2	348	352	PF04695	0.447
LIG_Pex14_2	474	478	PF04695	0.452
LIG_SH2_CRK	111	115	PF00017	0.386
LIG_SH2_CRK	318	322	PF00017	0.448
LIG_SH2_CRK	325	329	PF00017	0.449
LIG_SH2_CRK	504	508	PF00017	0.333
LIG_SH2_CRK	552	556	PF00017	0.384
LIG_SH2_NCK_1	318	322	PF00017	0.469
LIG_SH2_PTP2	532	535	PF00017	0.371
LIG_SH2_SRC	626	629	PF00017	0.367
LIG_SH2_STAP1	318	322	PF00017	0.466
LIG_SH2_STAP1	502	506	PF00017	0.308
LIG_SH2_STAP1	552	556	PF00017	0.310
LIG_SH2_STAT3	332	335	PF00017	0.530
LIG_SH2_STAT3	586	589	PF00017	0.494
LIG_SH2_STAT5	166	169	PF00017	0.464
LIG_SH2_STAT5	235	238	PF00017	0.470
LIG_SH2_STAT5	289	292	PF00017	0.461
LIG_SH2_STAT5	376	379	PF00017	0.483
LIG_SH2_STAT5	45	48	PF00017	0.328
LIG_SH2_STAT5	532	535	PF00017	0.321
LIG_SH2_STAT5	586	589	PF00017	0.441
LIG_SH2_STAT5	606	609	PF00017	0.328
LIG_SH2_STAT5	626	629	PF00017	0.340
LIG_SH3_3	576	582	PF00018	0.447
LIG_SH3_4	12	19	PF00018	0.512
LIG_SUMO_SIM_anti_2	516	523	PF11976	0.276
LIG_SUMO_SIM_par_1	282	288	PF11976	0.438
LIG_SUMO_SIM_par_1	555	561	PF11976	0.347
LIG_SUMO_SIM_par_1	626	632	PF11976	0.419
LIG_TYR_ITIM	109	114	PF00017	0.165
LIG_UBA3_1	216	223	PF00899	0.353
LIG_UBA3_1	35	40	PF00899	0.327
LIG_UBA3_1	365	374	PF00899	0.467
LIG_UBA3_1	519	525	PF00899	0.196
LIG_WRC_WIRS_1	507	512	PF05994	0.494
LIG_WRC_WIRS_1	658	663	PF05994	0.427
MOD_CDK_SPK_2	218	223	PF00069	0.523
MOD_CK1_1	23	29	PF00069	0.326
MOD_CK1_1	668	674	PF00069	0.477
MOD_CK2_1	309	315	PF00069	0.441
MOD_CK2_1	354	360	PF00069	0.467
MOD_CK2_1	436	442	PF00069	0.457
MOD_CK2_1	555	561	PF00069	0.373
MOD_CK2_1	68	74	PF00069	0.277
MOD_CMANNOS	475	478	PF00535	0.251
MOD_Cter_Amidation	692	695	PF01082	0.587
MOD_GlcNHglycan	111	114	PF01048	0.402
MOD_GlcNHglycan	132	135	PF01048	0.504
MOD_GlcNHglycan	180	183	PF01048	0.294
MOD_GlcNHglycan	22	25	PF01048	0.300
MOD_GlcNHglycan	277	280	PF01048	0.274
MOD_GlcNHglycan	395	398	PF01048	0.273
MOD_GlcNHglycan	442	446	PF01048	0.297
MOD_GlcNHglycan	486	489	PF01048	0.257
MOD_GlcNHglycan	80	83	PF01048	0.313
MOD_GSK3_1	167	174	PF00069	0.442
MOD_GSK3_1	178	185	PF00069	0.435
MOD_GSK3_1	20	27	PF00069	0.322
MOD_GSK3_1	285	292	PF00069	0.496
MOD_GSK3_1	354	361	PF00069	0.477
MOD_GSK3_1	543	550	PF00069	0.494
MOD_GSK3_1	588	595	PF00069	0.355
MOD_GSK3_1	631	638	PF00069	0.453
MOD_GSK3_1	664	671	PF00069	0.372
MOD_GSK3_1	78	85	PF00069	0.335
MOD_N-GLC_1	218	223	PF02516	0.153
MOD_N-GLC_1	275	280	PF02516	0.262
MOD_N-GLC_1	47	52	PF02516	0.295
MOD_N-GLC_1	523	528	PF02516	0.420
MOD_N-GLC_1	564	569	PF02516	0.453
MOD_N-GLC_1	573	578	PF02516	0.372
MOD_N-GLC_1	62	67	PF02516	0.297
MOD_N-GLC_1	78	83	PF02516	0.318
MOD_N-GLC_2	3	5	PF02516	0.407
MOD_NEK2_1	178	183	PF00069	0.409
MOD_NEK2_1	275	280	PF00069	0.476
MOD_NEK2_1	309	314	PF00069	0.453
MOD_NEK2_1	351	356	PF00069	0.507
MOD_NEK2_1	365	370	PF00069	0.474
MOD_NEK2_1	484	489	PF00069	0.445
MOD_NEK2_1	588	593	PF00069	0.409
MOD_NEK2_1	613	618	PF00069	0.542
MOD_NEK2_2	531	536	PF00069	0.433
MOD_NEK2_2	86	91	PF00069	0.289
MOD_NMyristoyl	1	7	PF02799	0.492
MOD_PIKK_1	172	178	PF00454	0.514
MOD_PIKK_1	223	229	PF00454	0.491
MOD_PIKK_1	260	266	PF00454	0.408
MOD_PIKK_1	47	53	PF00454	0.332
MOD_PIKK_1	524	530	PF00454	0.419
MOD_PIKK_1	564	570	PF00454	0.534
MOD_PIKK_1	629	635	PF00454	0.490
MOD_Plk_1	167	173	PF00069	0.453
MOD_Plk_4	174	180	PF00069	0.457
MOD_Plk_4	285	291	PF00069	0.491
MOD_Plk_4	413	419	PF00069	0.465
MOD_Plk_4	592	598	PF00069	0.381
MOD_Plk_4	668	674	PF00069	0.503
MOD_Plk_4	86	92	PF00069	0.322
MOD_ProDKin_1	218	224	PF00069	0.442
MOD_ProDKin_1	24	30	PF00069	0.320
MOD_ProDKin_1	379	385	PF00069	0.466
MOD_ProDKin_1	448	454	PF00069	0.474
MOD_ProDKin_1	662	668	PF00069	0.362
MOD_SUMO_rev_2	10	17	PF00179	0.501
MOD_SUMO_rev_2	546	553	PF00179	0.455
MOD_SUMO_rev_2	89	98	PF00179	0.330
TRG_DiLeu_BaLyEn_6	151	156	PF01217	0.256
TRG_ENDOCYTIC_2	111	114	PF00928	0.297
TRG_ENDOCYTIC_2	318	321	PF00928	0.450
TRG_ENDOCYTIC_2	325	328	PF00928	0.447
TRG_ENDOCYTIC_2	415	418	PF00928	0.471
TRG_ENDOCYTIC_2	457	460	PF00928	0.416
TRG_ENDOCYTIC_2	504	507	PF00928	0.324
TRG_ENDOCYTIC_2	532	535	PF00928	0.339
TRG_ENDOCYTIC_2	552	555	PF00928	0.352
TRG_ENDOCYTIC_2	597	600	PF00928	0.376
TRG_ENDOCYTIC_2	606	609	PF00928	0.306
TRG_ER_diArg_1	199	201	PF00400	0.484
TRG_ER_diArg_1	492	494	PF00400	0.474
TRG_ER_diArg_1	610	612	PF00400	0.451
TRG_NLS_Bipartite_1	611	627	PF00514	0.544
TRG_NLS_MonoExtC_3	361	367	PF00514	0.346
TRG_NLS_MonoExtC_3	690	695	PF00514	0.531
TRG_NLS_MonoExtN_4	622	627	PF00514	0.469
TRG_Pf-PMV_PEXEL_1	140	144	PF00026	0.541

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P8T6	Leptomonas seymouri	36%	100%
A0A0N1I8N2	Leptomonas seymouri	28%	72%
A0A0N1IGQ2	Leptomonas seymouri	70%	100%
A0A0N1ILF1	Leptomonas seymouri	34%	100%
A0A0N1IMH1	Leptomonas seymouri	33%	90%
A0A0N1P9P1	Leptomonas seymouri	30%	83%
A0A0N1PCA9	Leptomonas seymouri	28%	96%
A0A0N1PE91	Leptomonas seymouri	23%	79%
A0A0N1PFI4	Leptomonas seymouri	32%	91%
A0A0S4JLK6	Bodo saltans	33%	87%
A0A0S4JS70	Bodo saltans	24%	92%
A0A0S4KGT2	Bodo saltans	38%	93%
A0A1X0NJ61	Trypanosomatidae	45%	100%
A0A1X0NJK2	Trypanosomatidae	33%	93%
A0A1X0NJX8	Trypanosomatidae	34%	91%
A0A1X0NKT7	Trypanosomatidae	28%	89%
A0A1X0NKX8	Trypanosomatidae	32%	86%
A0A1X0NMT3	Trypanosomatidae	33%	83%
A0A1X0NW84	Trypanosomatidae	34%	100%
A0A1X0NW85	Trypanosomatidae	31%	100%
A0A1X0NW89	Trypanosomatidae	34%	82%
A0A1X0NWA6	Trypanosomatidae	27%	81%
A0A1X0NWW1	Trypanosomatidae	34%	98%
A0A3Q8IBS3	Leishmania donovani	34%	77%
A0A3Q8IDD4	Leishmania donovani	97%	100%
A0A3Q8IJT4	Leishmania donovani	25%	94%
A0A3S5H5A5	Leishmania donovani	31%	82%
A0A3S5ISG2	Trypanosoma rangeli	32%	91%
A0A3S7WW18	Leishmania donovani	34%	86%
A0A3S7WW41	Leishmania donovani	29%	95%
A0A3S7WW71	Leishmania donovani	35%	100%
A0A3S7X430	Leishmania donovani	33%	90%
A0A3S7X438	Leishmania donovani	28%	75%
A0A3S7X460	Leishmania donovani	35%	91%
A0A3S7X463	Leishmania donovani	27%	75%
A0A3S7X470	Leishmania donovani	32%	97%
A0A422MYU1	Trypanosoma rangeli	33%	77%
A0A422MYX0	Trypanosoma rangeli	33%	97%
A4H3W4	Leishmania braziliensis	31%	82%
A4HE81	Leishmania braziliensis	83%	100%
A4HJ14	Leishmania braziliensis	33%	90%
A4HJ21	Leishmania braziliensis	24%	79%
A4HJ22	Leishmania braziliensis	31%	74%
A4HJ23	Leishmania braziliensis	32%	100%
A4HJ24	Leishmania braziliensis	36%	99%
A4HS39	Leishmania infantum	31%	82%
A4HYN0	Leishmania infantum	34%	86%
A4HYW1	Leishmania infantum	34%	70%
A4HYW2	Leishmania infantum	35%	100%
A4HYW3	Leishmania infantum	29%	95%
A4HYW4	Leishmania infantum	25%	71%
A4I1J4	Leishmania infantum	97%	100%
A4I6E4	Leishmania infantum	32%	97%
A4I6E6	Leishmania infantum	35%	91%
A4I6F0	Leishmania infantum	28%	75%
A4I6K4	Leishmania infantum	33%	90%
A4I6K5	Leishmania infantum	25%	75%
A4I6K6	Leishmania infantum	25%	94%
C9ZIE8	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	32%	100%
C9ZIE9	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	33%	96%
C9ZN52	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	33%	89%
C9ZN53	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	29%	83%
C9ZWY4	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	29%	88%
C9ZY36	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	32%	82%
E8NHF1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	40%	100%
E8NHG6	Leishmania mexicana (strain MHOM/GT/2001/U1103)	31%	97%
E8NHG7	Leishmania mexicana (strain MHOM/GT/2001/U1103)	28%	75%
E8NHG8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	36%	90%
E8NHM2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	36%	100%
E8NHM4	Leishmania mexicana (strain MHOM/GT/2001/U1103)	36%	100%
E8NHM8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	38%	100%
E9AIH1	Leishmania braziliensis	34%	86%
E9AIH3	Leishmania braziliensis	33%	100%
E9AIH4	Leishmania braziliensis	30%	95%
E9AIH6	Leishmania braziliensis	24%	100%
E9AK26	Leishmania mexicana (strain MHOM/GT/2001/U1103)	30%	88%
E9AUQ7	Leishmania mexicana (strain MHOM/GT/2001/U1103)	34%	75%
E9AUQ9	Leishmania mexicana (strain MHOM/GT/2001/U1103)	35%	100%
E9AUR0	Leishmania mexicana (strain MHOM/GT/2001/U1103)	29%	95%
E9AUR1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	25%	100%
E9AXM9	Leishmania mexicana (strain MHOM/GT/2001/U1103)	92%	100%
E9B1J0	Leishmania mexicana (strain MHOM/GT/2001/U1103)	33%	90%
E9B1J1	Leishmania mexicana (strain MHOM/GT/2001/U1103)	27%	75%
E9B1J2	Leishmania mexicana (strain MHOM/GT/2001/U1103)	22%	94%
E9B1J6	Leishmania mexicana (strain MHOM/GT/2001/U1103)	36%	100%
Q22036	Caenorhabditis elegans	27%	100%
Q4Q6L7	Leishmania major	35%	96%
Q4Q6L9	Leishmania major	32%	98%
Q4Q6M0	Leishmania major	29%	75%
Q4Q6M2	Leishmania major	22%	94%
Q4Q6M3	Leishmania major	27%	76%
Q4Q6M4	Leishmania major	33%	82%
Q4QCS6	Leishmania major	29%	95%
Q4QCS7	Leishmania major	35%	100%
Q4QCS8	Leishmania major	34%	86%
Q4QCS9	Leishmania major	34%	72%
Q9U0T9	Leishmania major	30%	83%
V5AYJ1	Trypanosoma cruzi	35%	99%
V5B5I4	Trypanosoma cruzi	33%	84%
V5BA05	Trypanosoma cruzi	34%	100%
V5BEL3	Trypanosoma cruzi	34%	100%
V5BN20	Trypanosoma cruzi	33%	91%
V5D5V8	Trypanosoma cruzi	29%	95%
V5D9Y2	Trypanosoma cruzi	30%	83%
V5DES7	Trypanosoma cruzi	31%	89%