Putative diacylglycerol acyltransferase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

diacylglycerol acyltransferase, putative

Species:

Leishmania major

UniProt:

Q4Q9S8_LEIMA

TriTrypDb:

LmjF.25.1630 , LMJLV39_250023700 , LMJSD75_250023700

Length:

611

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 9

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 8
NetGPI	no	yes: 0, no: 8

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	2
GO:0005789	endoplasmic reticulum membrane	4	4
GO:0016020	membrane	2	8
GO:0031090	organelle membrane	3	4
GO:0032838	plasma membrane bounded cell projection cytoplasm	4	2
GO:0097014	ciliary plasm	5	2
GO:0099568	cytoplasmic region	3	2
GO:0110165	cellular anatomical entity	1	8

Expansion

Sequence features

Q4Q9S8

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4Q9S8

Function

Biological processes
Term	Name	Level	Count
GO:0006629	lipid metabolic process	3	2
GO:0006638	neutral lipid metabolic process	4	2
GO:0006639	acylglycerol metabolic process	5	2
GO:0006641	triglyceride metabolic process	6	2
GO:0006643	membrane lipid metabolic process	4	2
GO:0008152	metabolic process	1	2
GO:0008610	lipid biosynthetic process	4	2
GO:0009058	biosynthetic process	2	2
GO:0009987	cellular process	1	2
GO:0019432	triglyceride biosynthetic process	6	2
GO:0044237	cellular metabolic process	2	2
GO:0044238	primary metabolic process	2	2
GO:0044249	cellular biosynthetic process	3	2
GO:0044255	cellular lipid metabolic process	3	2
GO:0045017	glycerolipid biosynthetic process	4	2
GO:0046460	neutral lipid biosynthetic process	4	2
GO:0046463	acylglycerol biosynthetic process	5	2
GO:0046467	membrane lipid biosynthetic process	4	2
GO:0046486	glycerolipid metabolic process	4	2
GO:0071704	organic substance metabolic process	2	2
GO:1901576	organic substance biosynthetic process	3	2

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	9
GO:0004144	diacylglycerol O-acyltransferase activity	7	5
GO:0008374	O-acyltransferase activity	5	9
GO:0016411	acylglycerol O-acyltransferase activity	6	5
GO:0016740	transferase activity	2	9
GO:0016746	acyltransferase activity	3	9
GO:0016747	acyltransferase activity, transferring groups other than amino-acyl groups	4	9

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	402	406	PF00656	0.566
CLV_NRD_NRD_1	152	154	PF00675	0.317
CLV_NRD_NRD_1	289	291	PF00675	0.328
CLV_NRD_NRD_1	373	375	PF00675	0.329
CLV_NRD_NRD_1	446	448	PF00675	0.275
CLV_NRD_NRD_1	482	484	PF00675	0.270
CLV_NRD_NRD_1	54	56	PF00675	0.535
CLV_NRD_NRD_1	554	556	PF00675	0.493
CLV_NRD_NRD_1	589	591	PF00675	0.566
CLV_NRD_NRD_1	93	95	PF00675	0.525
CLV_PCSK_KEX2_1	152	154	PF00082	0.367
CLV_PCSK_KEX2_1	240	242	PF00082	0.475
CLV_PCSK_KEX2_1	289	291	PF00082	0.328
CLV_PCSK_KEX2_1	372	374	PF00082	0.328
CLV_PCSK_KEX2_1	482	484	PF00082	0.279
CLV_PCSK_KEX2_1	53	55	PF00082	0.541
CLV_PCSK_KEX2_1	554	556	PF00082	0.493
CLV_PCSK_KEX2_1	93	95	PF00082	0.510
CLV_PCSK_PC1ET2_1	240	242	PF00082	0.509
CLV_PCSK_PC1ET2_1	482	484	PF00082	0.306
CLV_PCSK_SKI1_1	152	156	PF00082	0.353
CLV_PCSK_SKI1_1	488	492	PF00082	0.269
CLV_PCSK_SKI1_1	541	545	PF00082	0.388
DEG_APCC_DBOX_1	152	160	PF00400	0.588
DEG_Nend_UBRbox_2	1	3	PF02207	0.735
DOC_CKS1_1	190	195	PF01111	0.792
DOC_CKS1_1	283	288	PF01111	0.631
DOC_CYCLIN_yClb5_NLxxxL_5	421	428	PF00134	0.511
DOC_MAPK_gen_1	289	296	PF00069	0.568
DOC_MAPK_gen_1	482	491	PF00069	0.455
DOC_MAPK_gen_1	554	561	PF00069	0.293
DOC_MAPK_HePTP_8	479	491	PF00069	0.528
DOC_MAPK_MEF2A_6	289	298	PF00069	0.512
DOC_MAPK_MEF2A_6	421	428	PF00069	0.483
DOC_MAPK_MEF2A_6	482	491	PF00069	0.471
DOC_MAPK_MEF2A_6	554	561	PF00069	0.269
DOC_PP2B_LxvP_1	182	185	PF13499	0.664
DOC_PP2B_LxvP_1	365	368	PF13499	0.469
DOC_PP2B_LxvP_1	424	427	PF13499	0.528
DOC_PP2B_LxvP_1	565	568	PF13499	0.328
DOC_PP4_FxxP_1	335	338	PF00568	0.407
DOC_PP4_FxxP_1	533	536	PF00568	0.325
DOC_USP7_MATH_1	171	175	PF00917	0.704
DOC_USP7_MATH_1	207	211	PF00917	0.722
DOC_USP7_MATH_1	30	34	PF00917	0.725
DOC_USP7_MATH_1	441	445	PF00917	0.493
DOC_USP7_MATH_1	454	458	PF00917	0.464
DOC_USP7_MATH_1	465	469	PF00917	0.448
DOC_USP7_UBL2_3	484	488	PF12436	0.528
DOC_WW_Pin1_4	189	194	PF00397	0.725
DOC_WW_Pin1_4	19	24	PF00397	0.761
DOC_WW_Pin1_4	241	246	PF00397	0.653
DOC_WW_Pin1_4	25	30	PF00397	0.760
DOC_WW_Pin1_4	282	287	PF00397	0.632
DOC_WW_Pin1_4	492	497	PF00397	0.493
LIG_14-3-3_CanoR_1	14	21	PF00244	0.718
LIG_14-3-3_CanoR_1	141	151	PF00244	0.593
LIG_14-3-3_CanoR_1	289	295	PF00244	0.514
LIG_14-3-3_CanoR_1	319	323	PF00244	0.328
LIG_14-3-3_CanoR_1	447	451	PF00244	0.493
LIG_14-3-3_CanoR_1	53	58	PF00244	0.706
LIG_ActinCP_TwfCPI_2	335	344	PF01115	0.407
LIG_BRCT_BRCA1_1	266	270	PF00533	0.629
LIG_BRCT_BRCA1_1	65	69	PF00533	0.728
LIG_DLG_GKlike_1	290	298	PF00625	0.528
LIG_eIF4E_1	360	366	PF01652	0.469
LIG_FHA_1	195	201	PF00498	0.703
LIG_FHA_1	310	316	PF00498	0.418
LIG_FHA_1	412	418	PF00498	0.504
LIG_FHA_1	493	499	PF00498	0.493
LIG_FHA_1	60	66	PF00498	0.729
LIG_FHA_2	43	49	PF00498	0.734
LIG_FHA_2	572	578	PF00498	0.269
LIG_LIR_Apic_2	210	214	PF02991	0.718
LIG_LIR_Apic_2	318	323	PF02991	0.320
LIG_LIR_Apic_2	332	338	PF02991	0.300
LIG_LIR_Gen_1	278	286	PF02991	0.674
LIG_LIR_Gen_1	297	307	PF02991	0.332
LIG_LIR_Gen_1	321	330	PF02991	0.390
LIG_LIR_Gen_1	343	353	PF02991	0.485
LIG_LIR_Gen_1	66	76	PF02991	0.736
LIG_LIR_LC3C_4	457	462	PF02991	0.493
LIG_LIR_Nem_3	278	282	PF02991	0.569
LIG_LIR_Nem_3	297	302	PF02991	0.449
LIG_LIR_Nem_3	343	348	PF02991	0.468
LIG_LIR_Nem_3	359	363	PF02991	0.470
LIG_LIR_Nem_3	378	384	PF02991	0.528
LIG_LIR_Nem_3	392	396	PF02991	0.493
LIG_LIR_Nem_3	517	522	PF02991	0.492
LIG_LIR_Nem_3	66	72	PF02991	0.707
LIG_NRBOX	290	296	PF00104	0.399
LIG_Pex14_1	331	335	PF04695	0.407
LIG_Pex14_1	581	585	PF04695	0.269
LIG_Pex14_2	348	352	PF04695	0.535
LIG_PTB_Apo_2	542	549	PF02174	0.407
LIG_PTB_Phospho_1	542	548	PF10480	0.407
LIG_SH2_CRK	320	324	PF00017	0.401
LIG_SH2_CRK	548	552	PF00017	0.328
LIG_SH2_NCK_1	320	324	PF00017	0.383
LIG_SH2_NCK_1	509	513	PF00017	0.516
LIG_SH2_PTP2	425	428	PF00017	0.566
LIG_SH2_STAT5	142	145	PF00017	0.567
LIG_SH2_STAT5	260	263	PF00017	0.569
LIG_SH2_STAT5	320	323	PF00017	0.390
LIG_SH2_STAT5	328	331	PF00017	0.293
LIG_SH2_STAT5	357	360	PF00017	0.487
LIG_SH2_STAT5	381	384	PF00017	0.469
LIG_SH2_STAT5	425	428	PF00017	0.528
LIG_SH2_STAT5	522	525	PF00017	0.520
LIG_SH2_STAT5	530	533	PF00017	0.316
LIG_SH2_STAT5	585	588	PF00017	0.269
LIG_SH3_3	15	21	PF00018	0.770
LIG_SH3_3	182	188	PF00018	0.663
LIG_SH3_3	197	203	PF00018	0.725
LIG_SH3_3	210	216	PF00018	0.713
LIG_SH3_3	26	32	PF00018	0.623
LIG_SH3_3	335	341	PF00018	0.325
LIG_SH3_3	381	387	PF00018	0.570
LIG_SH3_3	493	499	PF00018	0.493
LIG_SH3_3	558	564	PF00018	0.277
LIG_SH3_3	565	571	PF00018	0.259
LIG_SUMO_SIM_anti_2	312	318	PF11976	0.334
LIG_SUMO_SIM_anti_2	457	462	PF11976	0.471
LIG_SUMO_SIM_par_1	293	300	PF11976	0.363
LIG_SUMO_SIM_par_1	312	318	PF11976	0.147
LIG_SUMO_SIM_par_1	557	563	PF11976	0.293
LIG_TRAF2_1	115	118	PF00917	0.613
LIG_TRAF2_1	490	493	PF00917	0.469
LIG_TRAF2_1	95	98	PF00917	0.706
LIG_TRFH_1	548	552	PF08558	0.316
LIG_UBA3_1	523	528	PF00899	0.522
LIG_WRC_WIRS_1	298	303	PF05994	0.416
LIG_WRC_WIRS_1	345	350	PF05994	0.543
LIG_WRC_WIRS_1	390	395	PF05994	0.493
MOD_CDK_SPK_2	241	246	PF00069	0.653
MOD_CDK_SPxK_1	189	195	PF00069	0.788
MOD_CDK_SPxK_1	19	25	PF00069	0.746
MOD_CDK_SPxxK_3	282	289	PF00069	0.621
MOD_CK1_1	176	182	PF00069	0.686
MOD_CK1_1	234	240	PF00069	0.704
MOD_CK1_1	266	272	PF00069	0.674
MOD_CK1_1	278	284	PF00069	0.636
MOD_CK1_1	297	303	PF00069	0.464
MOD_CK1_1	318	324	PF00069	0.366
MOD_CK1_1	56	62	PF00069	0.692
MOD_CK1_1	63	69	PF00069	0.691
MOD_CK1_1	77	83	PF00069	0.560
MOD_CK2_1	112	118	PF00069	0.615
MOD_Cter_Amidation	238	241	PF01082	0.555
MOD_DYRK1A_RPxSP_1	19	23	PF00069	0.759
MOD_GlcNHglycan	175	178	PF01048	0.484
MOD_GlcNHglycan	179	182	PF01048	0.462
MOD_GlcNHglycan	440	444	PF01048	0.269
MOD_GlcNHglycan	451	454	PF01048	0.269
MOD_GlcNHglycan	75	79	PF01048	0.502
MOD_GSK3_1	173	180	PF00069	0.670
MOD_GSK3_1	194	201	PF00069	0.797
MOD_GSK3_1	203	210	PF00069	0.742
MOD_GSK3_1	278	285	PF00069	0.615
MOD_GSK3_1	290	297	PF00069	0.427
MOD_GSK3_1	336	343	PF00069	0.372
MOD_GSK3_1	49	56	PF00069	0.710
MOD_GSK3_1	59	66	PF00069	0.686
MOD_GSK3_1	83	90	PF00069	0.772
MOD_NEK2_1	265	270	PF00069	0.549
MOD_NEK2_1	294	299	PF00069	0.325
MOD_NEK2_1	315	320	PF00069	0.467
MOD_NEK2_1	329	334	PF00069	0.285
MOD_NEK2_1	49	54	PF00069	0.613
MOD_NEK2_1	602	607	PF00069	0.328
MOD_NEK2_1	61	66	PF00069	0.631
MOD_OFUCOSY	438	445	PF10250	0.328
MOD_PIKK_1	13	19	PF00454	0.729
MOD_PIKK_1	56	62	PF00454	0.566
MOD_PIKK_1	80	86	PF00454	0.739
MOD_PKA_1	53	59	PF00069	0.653
MOD_PKA_2	13	19	PF00069	0.645
MOD_PKA_2	234	240	PF00069	0.712
MOD_PKA_2	318	324	PF00069	0.328
MOD_PKA_2	336	342	PF00069	0.528
MOD_PKA_2	446	452	PF00069	0.469
MOD_PKA_2	53	59	PF00069	0.737
MOD_Plk_1	465	471	PF00069	0.528
MOD_Plk_4	278	284	PF00069	0.632
MOD_Plk_4	290	296	PF00069	0.450
MOD_Plk_4	454	460	PF00069	0.482
MOD_Plk_4	465	471	PF00069	0.448
MOD_ProDKin_1	189	195	PF00069	0.728
MOD_ProDKin_1	19	25	PF00069	0.763
MOD_ProDKin_1	241	247	PF00069	0.642
MOD_ProDKin_1	282	288	PF00069	0.629
MOD_ProDKin_1	492	498	PF00069	0.493
TRG_DiLeu_BaLyEn_6	102	107	PF01217	0.607
TRG_DiLeu_BaLyEn_6	249	254	PF01217	0.580
TRG_ENDOCYTIC_2	345	348	PF00928	0.506
TRG_ENDOCYTIC_2	360	363	PF00928	0.454
TRG_ENDOCYTIC_2	381	384	PF00928	0.469
TRG_ENDOCYTIC_2	425	428	PF00928	0.528
TRG_ENDOCYTIC_2	522	525	PF00928	0.507
TRG_ENDOCYTIC_2	530	533	PF00928	0.297
TRG_ENDOCYTIC_2	548	551	PF00928	0.344
TRG_ER_diArg_1	139	142	PF00400	0.583
TRG_ER_diArg_1	151	153	PF00400	0.555
TRG_ER_diArg_1	289	291	PF00400	0.528
TRG_ER_diArg_1	371	374	PF00400	0.528
TRG_ER_diArg_1	53	55	PF00400	0.662
TRG_ER_diArg_1	538	541	PF00400	0.439
TRG_ER_diArg_1	554	556	PF00400	0.236
TRG_Pf-PMV_PEXEL_1	55	60	PF00026	0.505
TRG_Pf-PMV_PEXEL_1	590	595	PF00026	0.566
TRG_Pf-PMV_PEXEL_1	597	601	PF00026	0.483

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PE36	Leptomonas seymouri	56%	94%
A0A3R7NQK7	Trypanosoma rangeli	44%	100%
A0A3S7WZ82	Leishmania donovani	90%	100%
A4HEI1	Leishmania braziliensis	76%	100%
A4I1K9	Leishmania infantum	90%	100%
C9ZK51	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	42%	100%
E9AXP3	Leishmania mexicana (strain MHOM/GT/2001/U1103)	88%	100%
Q08650	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	32%	100%