Putative mitotic spindle checkpoint component

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Mad2-like HORMA domain containing protein

Species:

Leishmania major

UniProt:

Q4Q9S3_LEIMA

TriTrypDb:

LmjF.25.1670 , LMJLV39_250024100 , LMJSD75_250024100

Length:

201

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 9
NetGPI	no	yes: 0, no: 9

Cellular components
Term	Name	Level	Count
GO:0000776	kinetochore	3	2
GO:0005654	nucleoplasm	2	2
GO:0005737	cytoplasm	2	2
GO:0005930	axoneme	2	2
GO:0043226	organelle	2	10
GO:0043228	non-membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	10
GO:0043232	intracellular non-membrane-bounded organelle	4	2
GO:0099080	supramolecular complex	2	2
GO:0110165	cellular anatomical entity	1	10
GO:0005634	nucleus	5	8
GO:0043227	membrane-bounded organelle	3	8
GO:0043231	intracellular membrane-bounded organelle	4	8

Expansion

Sequence features

Q4Q9S3

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4Q9S3

Function

Biological processes
Term	Name	Level	Count
GO:0000075	cell cycle checkpoint signaling	4	10
GO:0007088	regulation of mitotic nuclear division	6	10
GO:0007093	mitotic cell cycle checkpoint signaling	4	10
GO:0007094	mitotic spindle assembly checkpoint signaling	6	10
GO:0007165	signal transduction	2	10
GO:0007346	regulation of mitotic cell cycle	5	10
GO:0009987	cellular process	1	10
GO:0010564	regulation of cell cycle process	5	10
GO:0010639	negative regulation of organelle organization	6	10
GO:0010948	negative regulation of cell cycle process	6	10
GO:0010965	regulation of mitotic sister chromatid separation	6	10
GO:0022402	cell cycle process	2	10
GO:0030071	regulation of mitotic metaphase/anaphase transition	7	10
GO:0031577	spindle checkpoint signaling	5	10
GO:0033043	regulation of organelle organization	5	10
GO:0033044	regulation of chromosome organization	6	10
GO:0033045	regulation of sister chromatid segregation	5	10
GO:0033046	negative regulation of sister chromatid segregation	6	10
GO:0033047	regulation of mitotic sister chromatid segregation	6	10
GO:0033048	negative regulation of mitotic sister chromatid segregation	7	10
GO:0035556	intracellular signal transduction	3	10
GO:0045786	negative regulation of cell cycle	5	10
GO:0045839	negative regulation of mitotic nuclear division	7	10
GO:0045841	negative regulation of mitotic metaphase/anaphase transition	8	10
GO:0045930	negative regulation of mitotic cell cycle	6	10
GO:0048519	negative regulation of biological process	3	10
GO:0048523	negative regulation of cellular process	4	10
GO:0050789	regulation of biological process	2	10
GO:0050794	regulation of cellular process	3	10
GO:0051128	regulation of cellular component organization	4	10
GO:0051129	negative regulation of cellular component organization	5	10
GO:0051726	regulation of cell cycle	4	10
GO:0051783	regulation of nuclear division	6	10
GO:0051784	negative regulation of nuclear division	7	10
GO:0051983	regulation of chromosome segregation	4	10
GO:0051985	negative regulation of chromosome segregation	5	10
GO:0065007	biological regulation	1	10
GO:0071173	spindle assembly checkpoint signaling	6	10
GO:0071174	mitotic spindle checkpoint signaling	5	10
GO:1901987	regulation of cell cycle phase transition	6	10
GO:1901988	negative regulation of cell cycle phase transition	7	10
GO:1901990	regulation of mitotic cell cycle phase transition	6	10
GO:1901991	negative regulation of mitotic cell cycle phase transition	7	10
GO:1902099	regulation of metaphase/anaphase transition of cell cycle	6	10
GO:1902100	negative regulation of metaphase/anaphase transition of cell cycle	7	10
GO:1903047	mitotic cell cycle process	3	10
GO:1905818	regulation of chromosome separation	5	10
GO:1905819	negative regulation of chromosome separation	6	10
GO:2000816	negative regulation of mitotic sister chromatid separation	7	10
GO:2001251	negative regulation of chromosome organization	7	10

Could not find GO molecular_function term for this entry.

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	156	160	PF00656	0.353
CLV_NRD_NRD_1	116	118	PF00675	0.323
CLV_NRD_NRD_1	79	81	PF00675	0.260
CLV_PCSK_KEX2_1	79	81	PF00082	0.374
CLV_PCSK_SKI1_1	132	136	PF00082	0.257
CLV_PCSK_SKI1_1	184	188	PF00082	0.402
DEG_SPOP_SBC_1	106	110	PF00917	0.285
DOC_MAPK_gen_1	44	52	PF00069	0.323
DOC_MAPK_gen_1	79	85	PF00069	0.264
DOC_MAPK_MEF2A_6	44	52	PF00069	0.260
DOC_MAPK_NFAT4_5	45	53	PF00069	0.374
DOC_USP7_MATH_1	105	109	PF00917	0.457
DOC_USP7_MATH_1	136	140	PF00917	0.374
LIG_14-3-3_CanoR_1	117	125	PF00244	0.333
LIG_14-3-3_CanoR_1	132	141	PF00244	0.183
LIG_14-3-3_CanoR_1	184	193	PF00244	0.262
LIG_EH1_1	22	30	PF00400	0.400
LIG_EVH1_2	36	40	PF00568	0.353
LIG_FHA_1	133	139	PF00498	0.257
LIG_FHA_1	16	22	PF00498	0.190
LIG_FHA_1	193	199	PF00498	0.407
LIG_FHA_1	3	9	PF00498	0.514
LIG_FHA_2	14	20	PF00498	0.310
LIG_FHA_2	154	160	PF00498	0.259
LIG_FHA_2	163	169	PF00498	0.374
LIG_IRF3_LxIS_1	46	53	PF10401	0.326
LIG_LIR_Gen_1	187	198	PF02991	0.366
LIG_LIR_Gen_1	38	48	PF02991	0.374
LIG_LIR_Nem_3	139	143	PF02991	0.386
LIG_LIR_Nem_3	18	23	PF02991	0.257
LIG_LIR_Nem_3	187	193	PF02991	0.322
LIG_LIR_Nem_3	38	43	PF02991	0.322
LIG_NRBOX	61	67	PF00104	0.317
LIG_Pex14_2	186	190	PF04695	0.317
LIG_PTB_Apo_2	24	31	PF02174	0.374
LIG_PTB_Phospho_1	24	30	PF10480	0.374
LIG_SH2_NCK_1	155	159	PF00017	0.374
LIG_SH2_STAT5	155	158	PF00017	0.360
LIG_SH2_STAT5	192	195	PF00017	0.207
LIG_SH2_STAT5	20	23	PF00017	0.369
LIG_SH2_STAT5	61	64	PF00017	0.257
LIG_SH3_3	166	172	PF00018	0.393
LIG_SH3_3	31	37	PF00018	0.353
LIG_SUMO_SIM_par_1	80	87	PF11976	0.319
LIG_WRC_WIRS_1	183	188	PF05994	0.257
MOD_CK1_1	13	19	PF00069	0.283
MOD_CK1_1	185	191	PF00069	0.321
MOD_CK1_1	90	96	PF00069	0.333
MOD_CK2_1	13	19	PF00069	0.310
MOD_CK2_1	162	168	PF00069	0.374
MOD_Cter_Amidation	115	118	PF01082	0.309
MOD_GlcNHglycan	114	117	PF01048	0.385
MOD_GlcNHglycan	156	159	PF01048	0.399
MOD_GlcNHglycan	52	55	PF01048	0.233
MOD_GSK3_1	112	119	PF00069	0.276
MOD_GSK3_1	132	139	PF00069	0.252
MOD_GSK3_1	182	189	PF00069	0.357
MOD_GSK3_1	2	9	PF00069	0.488
MOD_GSK3_1	46	53	PF00069	0.360
MOD_GSK3_1	83	90	PF00069	0.374
MOD_NEK2_1	1	6	PF00069	0.540
MOD_NEK2_1	10	15	PF00069	0.351
MOD_NEK2_1	112	117	PF00069	0.231
MOD_NEK2_1	153	158	PF00069	0.391
MOD_NEK2_1	186	191	PF00069	0.354
MOD_NEK2_1	46	51	PF00069	0.333
MOD_NEK2_2	74	79	PF00069	0.333
MOD_PIKK_1	142	148	PF00454	0.333
MOD_PKA_2	116	122	PF00069	0.333
MOD_PKA_2	176	182	PF00069	0.285
MOD_Plk_1	193	199	PF00069	0.327
MOD_Plk_4	136	142	PF00069	0.281
MOD_Plk_4	193	199	PF00069	0.403
MOD_Plk_4	46	52	PF00069	0.421
MOD_Plk_4	68	74	PF00069	0.356
MOD_SUMO_rev_2	176	183	PF00179	0.280
TRG_ENDOCYTIC_2	20	23	PF00928	0.344
TRG_ER_diArg_1	79	81	PF00400	0.374

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I9P7	Leptomonas seymouri	74%	100%
A0A1X0NYH2	Trypanosomatidae	54%	100%
A0A3Q8IGN4	Leishmania donovani	97%	100%
A0A3R7NLU2	Trypanosoma rangeli	50%	100%
A4HEH6	Leishmania braziliensis	88%	99%
A4I1L4	Leishmania infantum	97%	100%
C9ZK57	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	48%	100%
D3Z8D9	Rattus norvegicus	27%	95%
E9AXP8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	95%	100%
O14417	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	40%	99%
P40958	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	37%	100%
Q13257	Homo sapiens	42%	98%
Q28H85	Xenopus tropicalis	28%	95%
Q2KIP7	Bos taurus	27%	95%
Q4KWZ6	Gallus gallus	28%	95%
Q556Y9	Dictyostelium discoideum	45%	99%
Q568H3	Danio rerio	27%	95%
Q59VQ3	Candida albicans (strain SC5314 / ATCC MYA-2876)	36%	94%
Q8QFR4	Xenopus laevis	28%	95%
Q9D752	Mus musculus	27%	95%
Q9LU93	Arabidopsis thaliana	40%	96%
Q9UI95	Homo sapiens	28%	95%
Q9XFH3	Zea mays	41%	97%
Q9Z1B5	Mus musculus	42%	98%