Not3 domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

General negative regulator of transcription subunit 5

Species:

Leishmania major

UniProt:

Q4Q9Q5_LEIMA

TriTrypDb:

LmjF.25.1840 , LMJLV39_250025900 , LMJSD75_250025900

Length:

641

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	yes	yes: 20

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 8

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 7
NetGPI	no	yes: 0, no: 7

Cellular components
Term	Name	Level	Count
GO:0000932	P-body	5	2
GO:0005634	nucleus	5	8
GO:0005737	cytoplasm	2	8
GO:0010494	cytoplasmic stress granule	5	2
GO:0030015	CCR4-NOT core complex	3	8
GO:0032991	protein-containing complex	1	8
GO:0035770	ribonucleoprotein granule	3	2
GO:0036464	cytoplasmic ribonucleoprotein granule	4	2
GO:0043226	organelle	2	8
GO:0043227	membrane-bounded organelle	3	8
GO:0043228	non-membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	8
GO:0043231	intracellular membrane-bounded organelle	4	8
GO:0043232	intracellular non-membrane-bounded organelle	4	2
GO:0099080	supramolecular complex	2	2
GO:0110165	cellular anatomical entity	1	8
GO:0140535	intracellular protein-containing complex	2	8

Expansion

Sequence features

Q4Q9Q5

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4Q9Q5

Function

Biological processes
Term	Name	Level	Count
GO:0000289	nuclear-transcribed mRNA poly(A) tail shortening	8	2
GO:0000956	nuclear-transcribed mRNA catabolic process	7	2
GO:0006139	nucleobase-containing compound metabolic process	3	2
GO:0006355	regulation of DNA-templated transcription	6	8
GO:0006401	RNA catabolic process	5	2
GO:0006402	mRNA catabolic process	6	2
GO:0006725	cellular aromatic compound metabolic process	3	2
GO:0006807	nitrogen compound metabolic process	2	2
GO:0008152	metabolic process	1	2
GO:0009056	catabolic process	2	2
GO:0009057	macromolecule catabolic process	4	2
GO:0009889	regulation of biosynthetic process	4	8
GO:0009892	negative regulation of metabolic process	4	2
GO:0009987	cellular process	1	2
GO:0010468	regulation of gene expression	5	8
GO:0010556	regulation of macromolecule biosynthetic process	5	8
GO:0010605	negative regulation of macromolecule metabolic process	5	2
GO:0010629	negative regulation of gene expression	6	2
GO:0016070	RNA metabolic process	5	2
GO:0016071	mRNA metabolic process	6	2
GO:0019219	regulation of nucleobase-containing compound metabolic process	5	8
GO:0019222	regulation of metabolic process	3	8
GO:0019439	aromatic compound catabolic process	4	2
GO:0031323	regulation of cellular metabolic process	4	8
GO:0031326	regulation of cellular biosynthetic process	5	8
GO:0034641	cellular nitrogen compound metabolic process	3	2
GO:0034655	nucleobase-containing compound catabolic process	4	2
GO:0043170	macromolecule metabolic process	3	2
GO:0044237	cellular metabolic process	2	2
GO:0044238	primary metabolic process	2	2
GO:0044248	cellular catabolic process	3	2
GO:0044260	obsolete cellular macromolecule metabolic process	3	2
GO:0044265	obsolete cellular macromolecule catabolic process	4	2
GO:0044270	cellular nitrogen compound catabolic process	4	2
GO:0046483	heterocycle metabolic process	3	2
GO:0046700	heterocycle catabolic process	4	2
GO:0048519	negative regulation of biological process	3	2
GO:0050789	regulation of biological process	2	8
GO:0050794	regulation of cellular process	3	8
GO:0051171	regulation of nitrogen compound metabolic process	4	8
GO:0051252	regulation of RNA metabolic process	5	8
GO:0060255	regulation of macromolecule metabolic process	4	8
GO:0065007	biological regulation	1	8
GO:0071704	organic substance metabolic process	2	2
GO:0080090	regulation of primary metabolic process	4	8
GO:0090304	nucleic acid metabolic process	4	2
GO:1901360	organic cyclic compound metabolic process	3	2
GO:1901361	organic cyclic compound catabolic process	4	2
GO:1901575	organic substance catabolic process	3	2
GO:1903506	regulation of nucleic acid-templated transcription	7	8
GO:2001141	regulation of RNA biosynthetic process	6	8

Could not find GO molecular_function term for this entry.

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	114	118	PF00656	0.580
CLV_C14_Caspase3-7	20	24	PF00656	0.537
CLV_C14_Caspase3-7	339	343	PF00656	0.641
CLV_NRD_NRD_1	131	133	PF00675	0.291
CLV_NRD_NRD_1	151	153	PF00675	0.204
CLV_NRD_NRD_1	396	398	PF00675	0.749
CLV_NRD_NRD_1	413	415	PF00675	0.647
CLV_NRD_NRD_1	80	82	PF00675	0.333
CLV_PCSK_FUR_1	149	153	PF00082	0.333
CLV_PCSK_KEX2_1	151	153	PF00082	0.333
CLV_PCSK_KEX2_1	396	398	PF00082	0.749
CLV_PCSK_KEX2_1	413	415	PF00082	0.641
CLV_PCSK_KEX2_1	49	51	PF00082	0.291
CLV_PCSK_PC1ET2_1	396	398	PF00082	0.607
CLV_PCSK_PC1ET2_1	49	51	PF00082	0.333
CLV_PCSK_SKI1_1	13	17	PF00082	0.333
CLV_PCSK_SKI1_1	381	385	PF00082	0.864
CLV_PCSK_SKI1_1	50	54	PF00082	0.333
CLV_PCSK_SKI1_1	577	581	PF00082	0.333
CLV_PCSK_SKI1_1	96	100	PF00082	0.333
DEG_SCF_FBW7_1	470	476	PF00400	0.816
DEG_SPOP_SBC_1	282	286	PF00917	0.687
DEG_SPOP_SBC_1	456	460	PF00917	0.682
DEG_SPOP_SBC_1	600	604	PF00917	0.533
DOC_CKS1_1	470	475	PF01111	0.786
DOC_CYCLIN_yClb5_NLxxxL_5	508	517	PF00134	0.385
DOC_CYCLIN_yCln2_LP_2	305	308	PF00134	0.663
DOC_CYCLIN_yCln2_LP_2	461	467	PF00134	0.660
DOC_MAPK_gen_1	154	164	PF00069	0.514
DOC_MAPK_gen_1	226	233	PF00069	0.668
DOC_MAPK_gen_1	72	79	PF00069	0.537
DOC_MAPK_MEF2A_6	226	233	PF00069	0.630
DOC_PP2B_LxvP_1	305	308	PF13499	0.677
DOC_PP4_FxxP_1	220	223	PF00568	0.537
DOC_USP7_MATH_1	282	286	PF00917	0.791
DOC_USP7_MATH_1	287	291	PF00917	0.646
DOC_USP7_MATH_1	343	347	PF00917	0.571
DOC_USP7_MATH_1	36	40	PF00917	0.533
DOC_USP7_MATH_1	374	378	PF00917	0.696
DOC_USP7_MATH_1	449	453	PF00917	0.721
DOC_USP7_MATH_1	473	477	PF00917	0.808
DOC_USP7_MATH_1	61	65	PF00917	0.543
DOC_USP7_UBL2_3	247	251	PF12436	0.712
DOC_USP7_UBL2_3	49	53	PF12436	0.533
DOC_USP7_UBL2_3	496	500	PF12436	0.579
DOC_USP7_UBL2_3	72	76	PF12436	0.533
DOC_WW_Pin1_4	242	247	PF00397	0.655
DOC_WW_Pin1_4	251	256	PF00397	0.687
DOC_WW_Pin1_4	261	266	PF00397	0.764
DOC_WW_Pin1_4	273	278	PF00397	0.646
DOC_WW_Pin1_4	283	288	PF00397	0.766
DOC_WW_Pin1_4	309	314	PF00397	0.688
DOC_WW_Pin1_4	397	402	PF00397	0.716
DOC_WW_Pin1_4	404	409	PF00397	0.812
DOC_WW_Pin1_4	434	439	PF00397	0.662
DOC_WW_Pin1_4	466	471	PF00397	0.748
DOC_WW_Pin1_4	495	500	PF00397	0.652
LIG_14-3-3_CanoR_1	609	615	PF00244	0.564
LIG_BIR_II_1	1	5	PF00653	0.591
LIG_BIR_III_2	532	536	PF00653	0.506
LIG_BRCT_BRCA1_1	345	349	PF00533	0.660
LIG_BRCT_BRCA1_1	610	614	PF00533	0.533
LIG_EVH1_1	485	489	PF00568	0.668
LIG_FHA_1	186	192	PF00498	0.534
LIG_FHA_1	254	260	PF00498	0.664
LIG_FHA_1	359	365	PF00498	0.645
LIG_FHA_1	380	386	PF00498	0.722
LIG_FHA_1	398	404	PF00498	0.560
LIG_FHA_1	456	462	PF00498	0.717
LIG_FHA_1	477	483	PF00498	0.741
LIG_FHA_1	514	520	PF00498	0.491
LIG_FHA_2	332	338	PF00498	0.672
LIG_FHA_2	600	606	PF00498	0.540
LIG_FHA_2	611	617	PF00498	0.515
LIG_FHA_2	7	13	PF00498	0.506
LIG_Integrin_isoDGR_2	147	149	PF01839	0.333
LIG_LIR_Apic_2	218	223	PF02991	0.557
LIG_LIR_Gen_1	215	225	PF02991	0.492
LIG_LIR_Gen_1	23	33	PF02991	0.492
LIG_LIR_Gen_1	549	559	PF02991	0.533
LIG_LIR_Gen_1	611	617	PF02991	0.533
LIG_LIR_Nem_3	215	220	PF02991	0.492
LIG_LIR_Nem_3	23	28	PF02991	0.492
LIG_LIR_Nem_3	557	563	PF02991	0.504
LIG_LIR_Nem_3	569	573	PF02991	0.533
LIG_LIR_Nem_3	611	617	PF02991	0.494
LIG_LIR_Nem_3	632	636	PF02991	0.530
LIG_LIR_Nem_3	89	94	PF02991	0.533
LIG_PCNA_PIPBox_1	553	562	PF02747	0.516
LIG_PCNA_yPIPBox_3	548	560	PF02747	0.516
LIG_Pex14_2	617	621	PF04695	0.491
LIG_PTB_Apo_2	65	72	PF02174	0.533
LIG_PTB_Phospho_1	65	71	PF10480	0.533
LIG_REV1ctd_RIR_1	169	177	PF16727	0.537
LIG_SH2_NCK_1	525	529	PF00017	0.533
LIG_SH2_SRC	525	528	PF00017	0.533
LIG_SH2_STAT3	140	143	PF00017	0.533
LIG_SH2_STAT3	536	539	PF00017	0.533
LIG_SH2_STAT3	564	567	PF00017	0.491
LIG_SH2_STAT5	536	539	PF00017	0.533
LIG_SH2_STAT5	573	576	PF00017	0.491
LIG_SH2_STAT5	612	615	PF00017	0.556
LIG_SH2_STAT5	635	638	PF00017	0.564
LIG_SH3_1	226	232	PF00018	0.726
LIG_SH3_1	396	402	PF00018	0.687
LIG_SH3_1	521	527	PF00018	0.533
LIG_SH3_3	226	232	PF00018	0.737
LIG_SH3_3	240	246	PF00018	0.592
LIG_SH3_3	382	388	PF00018	0.669
LIG_SH3_3	396	402	PF00018	0.676
LIG_SH3_3	435	441	PF00018	0.704
LIG_SH3_3	467	473	PF00018	0.850
LIG_SH3_3	478	484	PF00018	0.617
LIG_SH3_3	521	527	PF00018	0.510
LIG_SH3_3	532	538	PF00018	0.466
LIG_SUMO_SIM_anti_2	207	213	PF11976	0.537
LIG_TRAF2_1	127	130	PF00917	0.607
LIG_TRAF2_1	392	395	PF00917	0.687
LIG_WRC_WIRS_1	217	222	PF05994	0.533
LIG_WRC_WIRS_1	62	67	PF05994	0.537
LIG_WRC_WIRS_1	88	93	PF05994	0.491
MOD_CDK_SPK_2	242	247	PF00069	0.702
MOD_CDK_SPK_2	266	271	PF00069	0.685
MOD_CDK_SPK_2	495	500	PF00069	0.584
MOD_CDK_SPxK_1	495	501	PF00069	0.575
MOD_CK1_1	245	251	PF00069	0.703
MOD_CK1_1	469	475	PF00069	0.712
MOD_CK1_1	476	482	PF00069	0.617
MOD_CK1_1	495	501	PF00069	0.389
MOD_CK2_1	115	121	PF00069	0.533
MOD_CK2_1	233	239	PF00069	0.677
MOD_CK2_1	266	272	PF00069	0.739
MOD_CK2_1	350	356	PF00069	0.666
MOD_CK2_1	36	42	PF00069	0.533
MOD_CK2_1	449	455	PF00069	0.698
MOD_CK2_1	599	605	PF00069	0.533
MOD_Cter_Amidation	130	133	PF01082	0.333
MOD_GlcNHglycan	109	112	PF01048	0.302
MOD_GlcNHglycan	117	120	PF01048	0.311
MOD_GlcNHglycan	345	348	PF01048	0.657
MOD_GlcNHglycan	358	361	PF01048	0.511
MOD_GlcNHglycan	38	41	PF01048	0.333
MOD_GlcNHglycan	450	454	PF01048	0.701
MOD_GlcNHglycan	475	478	PF01048	0.767
MOD_GSK3_1	212	219	PF00069	0.543
MOD_GSK3_1	242	249	PF00069	0.814
MOD_GSK3_1	251	258	PF00069	0.755
MOD_GSK3_1	261	268	PF00069	0.687
MOD_GSK3_1	283	290	PF00069	0.792
MOD_GSK3_1	343	350	PF00069	0.834
MOD_GSK3_1	358	365	PF00069	0.558
MOD_GSK3_1	379	386	PF00069	0.691
MOD_GSK3_1	465	472	PF00069	0.709
MOD_GSK3_1	599	606	PF00069	0.545
MOD_GSK3_1	97	104	PF00069	0.508
MOD_N-GLC_1	36	41	PF02516	0.297
MOD_NEK2_1	506	511	PF00069	0.600
MOD_NEK2_1	77	82	PF00069	0.533
MOD_NEK2_2	610	615	PF00069	0.533
MOD_PIKK_1	362	368	PF00454	0.645
MOD_PKA_2	608	614	PF00069	0.533
MOD_Plk_1	212	218	PF00069	0.540
MOD_Plk_1	336	342	PF00069	0.640
MOD_Plk_1	506	512	PF00069	0.325
MOD_Plk_2-3	185	191	PF00069	0.525
MOD_Plk_2-3	212	218	PF00069	0.533
MOD_Plk_4	173	179	PF00069	0.599
MOD_Plk_4	212	218	PF00069	0.546
MOD_Plk_4	457	463	PF00069	0.724
MOD_Plk_4	555	561	PF00069	0.506
MOD_Plk_4	61	67	PF00069	0.537
MOD_Plk_4	610	616	PF00069	0.533
MOD_ProDKin_1	242	248	PF00069	0.656
MOD_ProDKin_1	251	257	PF00069	0.684
MOD_ProDKin_1	261	267	PF00069	0.764
MOD_ProDKin_1	273	279	PF00069	0.649
MOD_ProDKin_1	283	289	PF00069	0.763
MOD_ProDKin_1	309	315	PF00069	0.688
MOD_ProDKin_1	397	403	PF00069	0.715
MOD_ProDKin_1	404	410	PF00069	0.810
MOD_ProDKin_1	434	440	PF00069	0.660
MOD_ProDKin_1	466	472	PF00069	0.749
MOD_ProDKin_1	495	501	PF00069	0.643
MOD_SUMO_for_1	172	175	PF00179	0.537
MOD_SUMO_for_1	48	51	PF00179	0.533
MOD_SUMO_rev_2	218	227	PF00179	0.674
MOD_SUMO_rev_2	234	243	PF00179	0.684
MOD_SUMO_rev_2	377	383	PF00179	0.752
MOD_SUMO_rev_2	45	55	PF00179	0.533
MOD_SUMO_rev_2	627	636	PF00179	0.491
MOD_SUMO_rev_2	95	100	PF00179	0.533
TRG_DiLeu_BaEn_1	10	15	PF01217	0.491
TRG_DiLeu_LyEn_5	10	15	PF01217	0.491
TRG_ENDOCYTIC_2	217	220	PF00928	0.491
TRG_ENDOCYTIC_2	25	28	PF00928	0.491
TRG_ENDOCYTIC_2	551	554	PF00928	0.491
TRG_ENDOCYTIC_2	594	597	PF00928	0.533
TRG_ENDOCYTIC_2	612	615	PF00928	0.533
TRG_ENDOCYTIC_2	633	636	PF00928	0.491
TRG_ER_diArg_1	149	152	PF00400	0.533
TRG_ER_diArg_1	521	524	PF00400	0.533
TRG_NES_CRM1_1	196	212	PF08389	0.537

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1IMJ8	Leptomonas seymouri	66%	95%
A0A3S5H7F0	Leishmania donovani	94%	100%
A4HE86	Leishmania braziliensis	87%	100%
A4I1N2	Leishmania infantum	94%	100%
C9ZK81	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	32%	100%
E9AXR6	Leishmania mexicana (strain MHOM/GT/2001/U1103)	93%	99%