Putative pumillio protein 8

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Pumilio homology domain family member 8

Species:

Leishmania major

UniProt:

Q4Q9K0_LEIMA

TriTrypDb:

LmjF.25.2360 , LMJLV39_250031300 , LMJSD75_250031600

Length:

573

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 11
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005730	nucleolus	5	2
GO:0043226	organelle	2	2
GO:0043228	non-membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043232	intracellular non-membrane-bounded organelle	4	2
GO:0110165	cellular anatomical entity	1	2

Expansion

Sequence features

Q4Q9K0

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4Q9K0

Function

Biological processes
Term	Name	Level	Count
GO:0006417	regulation of translation	6	2
GO:0009889	regulation of biosynthetic process	4	2
GO:0010468	regulation of gene expression	5	2
GO:0010556	regulation of macromolecule biosynthetic process	5	2
GO:0010608	post-transcriptional regulation of gene expression	6	2
GO:0019222	regulation of metabolic process	3	2
GO:0031323	regulation of cellular metabolic process	4	2
GO:0031326	regulation of cellular biosynthetic process	5	2
GO:0034248	regulation of amide metabolic process	5	2
GO:0050789	regulation of biological process	2	2
GO:0050794	regulation of cellular process	3	2
GO:0051171	regulation of nitrogen compound metabolic process	4	2
GO:0051246	regulation of protein metabolic process	5	2
GO:0060255	regulation of macromolecule metabolic process	4	2
GO:0065007	biological regulation	1	2
GO:0080090	regulation of primary metabolic process	4	2
GO:2000112	obsolete regulation of cellular macromolecule biosynthetic process	6	2

Molecular functions
Term	Name	Level	Count
GO:0003676	nucleic acid binding	3	12
GO:0003723	RNA binding	4	12
GO:0003729	mRNA binding	5	2
GO:0005488	binding	1	12
GO:0097159	organic cyclic compound binding	2	12
GO:1901363	heterocyclic compound binding	2	12

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	217	219	PF00675	0.501
CLV_NRD_NRD_1	237	239	PF00675	0.401
CLV_NRD_NRD_1	313	315	PF00675	0.364
CLV_NRD_NRD_1	351	353	PF00675	0.355
CLV_NRD_NRD_1	385	387	PF00675	0.350
CLV_NRD_NRD_1	388	390	PF00675	0.381
CLV_NRD_NRD_1	541	543	PF00675	0.706
CLV_NRD_NRD_1	560	562	PF00675	0.737
CLV_PCSK_FUR_1	386	390	PF00082	0.296
CLV_PCSK_KEX2_1	217	219	PF00082	0.372
CLV_PCSK_KEX2_1	237	239	PF00082	0.340
CLV_PCSK_KEX2_1	313	315	PF00082	0.388
CLV_PCSK_KEX2_1	387	389	PF00082	0.476
CLV_PCSK_KEX2_1	503	505	PF00082	0.593
CLV_PCSK_KEX2_1	525	527	PF00082	0.652
CLV_PCSK_KEX2_1	541	543	PF00082	0.630
CLV_PCSK_KEX2_1	560	562	PF00082	0.640
CLV_PCSK_KEX2_1	568	570	PF00082	0.473
CLV_PCSK_PC1ET2_1	313	315	PF00082	0.388
CLV_PCSK_PC1ET2_1	387	389	PF00082	0.351
CLV_PCSK_PC1ET2_1	503	505	PF00082	0.593
CLV_PCSK_PC1ET2_1	525	527	PF00082	0.685
CLV_PCSK_PC1ET2_1	541	543	PF00082	0.638
CLV_PCSK_PC1ET2_1	568	570	PF00082	0.500
CLV_PCSK_SKI1_1	129	133	PF00082	0.446
CLV_PCSK_SKI1_1	165	169	PF00082	0.441
CLV_PCSK_SKI1_1	201	205	PF00082	0.296
CLV_PCSK_SKI1_1	237	241	PF00082	0.335
CLV_PCSK_SKI1_1	315	319	PF00082	0.403
CLV_PCSK_SKI1_1	388	392	PF00082	0.385
CLV_PCSK_SKI1_1	44	48	PF00082	0.543
CLV_PCSK_SKI1_1	443	447	PF00082	0.365
CLV_PCSK_SKI1_1	468	472	PF00082	0.355
CLV_PCSK_SKI1_1	560	564	PF00082	0.707
CLV_PCSK_SKI1_1	568	572	PF00082	0.489
CLV_Separin_Metazoa	55	59	PF03568	0.369
DEG_APCC_DBOX_1	236	244	PF00400	0.393
DEG_APCC_DBOX_1	467	475	PF00400	0.427
DEG_SCF_FBW7_1	105	112	PF00400	0.347
DEG_SCF_FBW7_2	379	385	PF00400	0.335
DOC_CDC14_PxL_1	171	179	PF14671	0.327
DOC_CDC14_PxL_1	435	443	PF14671	0.375
DOC_CKS1_1	106	111	PF01111	0.357
DOC_CKS1_1	379	384	PF01111	0.310
DOC_CKS1_1	413	418	PF01111	0.373
DOC_CYCLIN_RxL_1	231	241	PF00134	0.411
DOC_MAPK_MEF2A_6	201	208	PF00069	0.307
DOC_MAPK_NFAT4_5	201	209	PF00069	0.307
DOC_PP1_RVXF_1	127	134	PF00149	0.333
DOC_PP4_FxxP_1	390	393	PF00568	0.393
DOC_USP7_MATH_1	109	113	PF00917	0.335
DOC_USP7_MATH_1	199	203	PF00917	0.425
DOC_USP7_MATH_1	300	304	PF00917	0.357
DOC_USP7_MATH_1	430	434	PF00917	0.413
DOC_USP7_MATH_1	75	79	PF00917	0.637
DOC_USP7_MATH_2	159	165	PF00917	0.409
DOC_USP7_UBL2_3	132	136	PF12436	0.477
DOC_USP7_UBL2_3	44	48	PF12436	0.428
DOC_USP7_UBL2_3	563	567	PF12436	0.693
DOC_USP7_UBL2_3	568	572	PF12436	0.542
DOC_WW_Pin1_4	105	110	PF00397	0.345
DOC_WW_Pin1_4	194	199	PF00397	0.488
DOC_WW_Pin1_4	366	371	PF00397	0.310
DOC_WW_Pin1_4	378	383	PF00397	0.305
DOC_WW_Pin1_4	412	417	PF00397	0.378
DOC_WW_Pin1_4	534	539	PF00397	0.649
LIG_14-3-3_CanoR_1	113	119	PF00244	0.322
LIG_14-3-3_CanoR_1	560	570	PF00244	0.651
LIG_14-3-3_CterR_2	569	573	PF00244	0.496
LIG_APCC_ABBA_1	206	211	PF00400	0.367
LIG_APCC_ABBA_1	380	385	PF00400	0.382
LIG_BIR_III_4	508	512	PF00653	0.578
LIG_BRCT_BRCA1_1	134	138	PF00533	0.365
LIG_BRCT_BRCA1_1	240	244	PF00533	0.391
LIG_BRCT_BRCA1_1	302	306	PF00533	0.350
LIG_Clathr_ClatBox_1	400	404	PF01394	0.396
LIG_deltaCOP1_diTrp_1	407	413	PF00928	0.403
LIG_EH1_1	148	156	PF00400	0.292
LIG_eIF4E_1	371	377	PF01652	0.393
LIG_FHA_1	106	112	PF00498	0.386
LIG_FHA_1	166	172	PF00498	0.468
LIG_FHA_1	420	426	PF00498	0.375
LIG_FHA_1	430	436	PF00498	0.339
LIG_FHA_2	272	278	PF00498	0.355
LIG_FHA_2	379	385	PF00498	0.354
LIG_FHA_2	535	541	PF00498	0.655
LIG_FHA_2	63	69	PF00498	0.478
LIG_LIR_Apic_2	192	198	PF02991	0.481
LIG_LIR_Apic_2	330	336	PF02991	0.322
LIG_LIR_Apic_2	412	416	PF02991	0.371
LIG_LIR_Gen_1	143	154	PF02991	0.388
LIG_LIR_Gen_1	161	171	PF02991	0.474
LIG_LIR_Gen_1	99	109	PF02991	0.520
LIG_LIR_Nem_3	135	141	PF02991	0.337
LIG_LIR_Nem_3	143	149	PF02991	0.281
LIG_LIR_Nem_3	161	166	PF02991	0.430
LIG_LIR_Nem_3	99	105	PF02991	0.445
LIG_LYPXL_yS_3	222	225	PF13949	0.345
LIG_LYPXL_yS_3	259	262	PF13949	0.389
LIG_LYPXL_yS_3	88	91	PF13949	0.355
LIG_NRBOX	340	346	PF00104	0.425
LIG_NRBOX	375	381	PF00104	0.332
LIG_PCNA_yPIPBox_3	263	275	PF02747	0.334
LIG_PTB_Apo_2	365	372	PF02174	0.313
LIG_PTB_Phospho_1	365	371	PF10480	0.411
LIG_Rb_pABgroove_1	238	246	PF01858	0.419
LIG_Rb_pABgroove_1	336	344	PF01858	0.277
LIG_RPA_C_Fungi	455	467	PF08784	0.512
LIG_SH2_CRK	163	167	PF00017	0.421
LIG_SH2_CRK	333	337	PF00017	0.284
LIG_SH2_CRK	373	377	PF00017	0.397
LIG_SH2_NCK_1	516	520	PF00017	0.575
LIG_SH2_STAP1	141	145	PF00017	0.378
LIG_SH2_STAP1	146	150	PF00017	0.363
LIG_SH2_STAP1	163	167	PF00017	0.392
LIG_SH2_STAP1	191	195	PF00017	0.330
LIG_SH2_STAP1	346	350	PF00017	0.349
LIG_SH2_STAP1	84	88	PF00017	0.427
LIG_SH2_STAT3	346	349	PF00017	0.357
LIG_SH2_STAT3	371	374	PF00017	0.446
LIG_SH2_STAT5	269	272	PF00017	0.336
LIG_SH2_STAT5	354	357	PF00017	0.300
LIG_SH2_STAT5	448	451	PF00017	0.509
LIG_SH3_1	217	223	PF00018	0.417
LIG_SH3_3	103	109	PF00018	0.414
LIG_SH3_3	169	175	PF00018	0.353
LIG_SH3_3	217	223	PF00018	0.417
LIG_SH3_3	364	370	PF00018	0.381
LIG_SH3_3	71	77	PF00018	0.476
LIG_SUMO_SIM_anti_2	202	207	PF11976	0.343
LIG_SUMO_SIM_anti_2	211	217	PF11976	0.316
LIG_SUMO_SIM_anti_2	26	32	PF11976	0.326
LIG_SUMO_SIM_par_1	421	427	PF11976	0.402
LIG_TRAF2_1	11	14	PF00917	0.549
LIG_TRAF2_1	382	385	PF00917	0.431
LIG_TYR_ITIM	257	262	PF00017	0.375
LIG_UBA3_1	203	210	PF00899	0.297
LIG_UBA3_1	243	249	PF00899	0.331
LIG_UBA3_1	400	406	PF00899	0.442
LIG_UBA3_1	56	62	PF00899	0.430
LIG_UBA3_1	94	101	PF00899	0.359
LIG_WRC_WIRS_1	318	323	PF05994	0.502
MOD_CDK_SPxxK_3	194	201	PF00069	0.480
MOD_CDK_SPxxK_3	534	541	PF00069	0.719
MOD_CK1_1	189	195	PF00069	0.392
MOD_CK1_1	79	85	PF00069	0.568
MOD_CK2_1	249	255	PF00069	0.446
MOD_CK2_1	271	277	PF00069	0.395
MOD_CK2_1	304	310	PF00069	0.321
MOD_CK2_1	378	384	PF00069	0.367
MOD_CK2_1	484	490	PF00069	0.475
MOD_Cter_Amidation	501	504	PF01082	0.663
MOD_Cter_Amidation	558	561	PF01082	0.719
MOD_GlcNHglycan	122	125	PF01048	0.495
MOD_GlcNHglycan	292	295	PF01048	0.260
MOD_GlcNHglycan	454	457	PF01048	0.476
MOD_GlcNHglycan	551	554	PF01048	0.660
MOD_GSK3_1	105	112	PF00069	0.340
MOD_GSK3_1	132	139	PF00069	0.468
MOD_GSK3_1	161	168	PF00069	0.380
MOD_GSK3_1	186	193	PF00069	0.327
MOD_GSK3_1	19	26	PF00069	0.432
MOD_GSK3_1	300	307	PF00069	0.348
MOD_GSK3_1	542	549	PF00069	0.689
MOD_GSK3_1	75	82	PF00069	0.739
MOD_N-GLC_1	527	532	PF02516	0.699
MOD_NEK2_1	1	6	PF00069	0.533
MOD_NEK2_1	265	270	PF00069	0.421
MOD_NEK2_1	281	286	PF00069	0.248
MOD_NEK2_1	304	309	PF00069	0.355
MOD_NEK2_1	328	333	PF00069	0.443
MOD_PIKK_1	249	255	PF00454	0.397
MOD_PIKK_1	527	533	PF00454	0.789
MOD_PK_1	107	113	PF00069	0.403
MOD_PKA_1	542	548	PF00069	0.772
MOD_PKA_2	14	20	PF00069	0.567
MOD_PKA_2	23	29	PF00069	0.436
MOD_Plk_1	136	142	PF00069	0.457
MOD_Plk_1	199	205	PF00069	0.451
MOD_Plk_2-3	67	73	PF00069	0.650
MOD_Plk_4	114	120	PF00069	0.351
MOD_Plk_4	186	192	PF00069	0.402
MOD_Plk_4	265	271	PF00069	0.515
MOD_Plk_4	409	415	PF00069	0.352
MOD_Plk_4	546	552	PF00069	0.502
MOD_ProDKin_1	105	111	PF00069	0.340
MOD_ProDKin_1	194	200	PF00069	0.484
MOD_ProDKin_1	366	372	PF00069	0.306
MOD_ProDKin_1	378	384	PF00069	0.308
MOD_ProDKin_1	412	418	PF00069	0.376
MOD_ProDKin_1	534	540	PF00069	0.646
MOD_SUMO_for_1	524	527	PF00179	0.815
TRG_DiLeu_BaLyEn_6	126	131	PF01217	0.432
TRG_DiLeu_BaLyEn_6	235	240	PF01217	0.344
TRG_ENDOCYTIC_2	146	149	PF00928	0.328
TRG_ENDOCYTIC_2	163	166	PF00928	0.426
TRG_ENDOCYTIC_2	222	225	PF00928	0.345
TRG_ENDOCYTIC_2	259	262	PF00928	0.366
TRG_ENDOCYTIC_2	373	376	PF00928	0.396
TRG_ENDOCYTIC_2	88	91	PF00928	0.355
TRG_ER_diArg_1	216	218	PF00400	0.283
TRG_ER_diArg_1	237	239	PF00400	0.410
TRG_ER_diArg_1	386	389	PF00400	0.301
TRG_ER_diArg_1	560	562	PF00400	0.640
TRG_NLS_Bipartite_1	525	547	PF00514	0.721
TRG_NLS_MonoExtC_3	541	546	PF00514	0.714
TRG_NLS_MonoExtC_3	566	571	PF00514	0.629
TRG_NLS_MonoExtN_4	386	391	PF00514	0.335
TRG_NLS_MonoExtN_4	541	547	PF00514	0.697
TRG_NLS_MonoExtN_4	567	572	PF00514	0.628
TRG_Pf-PMV_PEXEL_1	64	68	PF00026	0.514

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1HWV1	Leptomonas seymouri	83%	100%
A0A0S4IXV1	Bodo saltans	48%	82%
A0A1X0NZF1	Trypanosomatidae	60%	93%
A0A3Q8IDL8	Leishmania donovani	96%	100%
A0A3R7MC19	Trypanosoma rangeli	58%	98%
A4HED9	Leishmania braziliensis	84%	99%
A4I1T6	Leishmania infantum	96%	100%
C9ZKE5	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	52%	94%
E9AXX0	Leishmania mexicana (strain MHOM/GT/2001/U1103)	92%	100%
V5D8H4	Trypanosoma cruzi	55%	98%