Protein disulfide-isomerase

Quick info Annotations Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

protein disulfide isomerase, putative

Species:

Leishmania major

UniProt:

Q4Q9C8_LEIMA

TriTrypDb:

LmjF.26.0660 , LMJLV39_260012000 , LMJSD75_260010500

Length:

377

Annotations

LeishMANIAdb annotations

Appears to be a family of secreted proteins, probably ER-localized, like its homologs in other Eukaryotes.. Seems to carry a putative KDEL-like signal on its C-terminus.. Localization: Secreted (by homology)

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 4
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	yes	yes: 16

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	yes	yes: 9, no: 2
NetGPI	no	yes: 0, no: 11

Cellular components
Term	Name	Level	Count
GO:0005783	endoplasmic reticulum	5	12
GO:0005794	Golgi apparatus	5	2
GO:0043226	organelle	2	12
GO:0043227	membrane-bounded organelle	3	12
GO:0043229	intracellular organelle	3	12
GO:0043231	intracellular membrane-bounded organelle	4	12
GO:0110165	cellular anatomical entity	1	12

Expansion

Sequence features

Q4Q9C8

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4Q9C8

Function

Biological processes
Term	Name	Level	Count
GO:0006457	protein folding	2	2
GO:0009987	cellular process	1	2

Molecular functions
Term	Name	Level	Count
GO:0003756	protein disulfide isomerase activity	3	10
GO:0003824	catalytic activity	1	11
GO:0016853	isomerase activity	2	11
GO:0016860	intramolecular oxidoreductase activity	3	10
GO:0016864	intramolecular oxidoreductase activity, transposing S-S bonds	4	10
GO:0140096	catalytic activity, acting on a protein	2	10

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	357	361	PF00656	0.345
CLV_MEL_PAP_1	101	107	PF00089	0.402
CLV_NRD_NRD_1	220	222	PF00675	0.495
CLV_NRD_NRD_1	3	5	PF00675	0.624
CLV_NRD_NRD_1	358	360	PF00675	0.531
CLV_NRD_NRD_1	364	366	PF00675	0.531
CLV_PCSK_FUR_1	345	349	PF00082	0.532
CLV_PCSK_KEX2_1	103	105	PF00082	0.404
CLV_PCSK_KEX2_1	3	5	PF00082	0.620
CLV_PCSK_KEX2_1	347	349	PF00082	0.534
CLV_PCSK_KEX2_1	358	360	PF00082	0.435
CLV_PCSK_KEX2_1	364	366	PF00082	0.424
CLV_PCSK_PC1ET2_1	103	105	PF00082	0.404
CLV_PCSK_PC1ET2_1	347	349	PF00082	0.556
CLV_PCSK_SKI1_1	174	178	PF00082	0.346
CLV_PCSK_SKI1_1	189	193	PF00082	0.335
CLV_PCSK_SKI1_1	222	226	PF00082	0.404
CLV_PCSK_SKI1_1	50	54	PF00082	0.455
CLV_PCSK_SKI1_1	85	89	PF00082	0.413
DEG_SPOP_SBC_1	293	297	PF00917	0.304
DOC_CYCLIN_yClb5_NLxxxL_5	253	259	PF00134	0.404
DOC_CYCLIN_yCln2_LP_2	148	154	PF00134	0.350
DOC_MAPK_gen_1	3	11	PF00069	0.614
DOC_MAPK_gen_1	48	56	PF00069	0.482
DOC_MAPK_MEF2A_6	3	11	PF00069	0.607
DOC_MAPK_MEF2A_6	48	56	PF00069	0.383
DOC_PP2B_LxvP_1	148	151	PF13499	0.426
DOC_PP4_FxxP_1	180	183	PF00568	0.335
DOC_PP4_FxxP_1	56	59	PF00568	0.335
DOC_SPAK_OSR1_1	104	108	PF12202	0.327
DOC_USP7_MATH_1	294	298	PF00917	0.333
DOC_USP7_UBL2_3	170	174	PF12436	0.444
LIG_14-3-3_CanoR_1	221	229	PF00244	0.404
LIG_14-3-3_CanoR_1	3	8	PF00244	0.599
LIG_14-3-3_CanoR_1	345	351	PF00244	0.388
LIG_Actin_WH2_2	132	149	PF00022	0.469
LIG_FHA_1	147	153	PF00498	0.440
LIG_FHA_2	129	135	PF00498	0.444
LIG_FHA_2	161	167	PF00498	0.473
LIG_Integrin_isoDGR_2	251	253	PF01839	0.404
LIG_LIR_Apic_2	112	118	PF02991	0.435
LIG_LIR_Apic_2	234	239	PF02991	0.482
LIG_LIR_Apic_2	55	59	PF02991	0.327
LIG_LIR_Gen_1	163	172	PF02991	0.348
LIG_LIR_Gen_1	256	266	PF02991	0.446
LIG_LIR_Gen_1	40	46	PF02991	0.455
LIG_LIR_Gen_1	70	78	PF02991	0.370
LIG_LIR_Nem_3	108	113	PF02991	0.410
LIG_LIR_Nem_3	133	138	PF02991	0.358
LIG_LIR_Nem_3	163	168	PF02991	0.348
LIG_LIR_Nem_3	256	261	PF02991	0.446
LIG_LIR_Nem_3	40	45	PF02991	0.455
LIG_LIR_Nem_3	70	74	PF02991	0.351
LIG_Pex14_2	180	184	PF04695	0.337
LIG_Pex14_2	56	60	PF04695	0.312
LIG_PTB_Apo_2	270	277	PF02174	0.316
LIG_RPA_C_Fungi	207	219	PF08784	0.404
LIG_SH2_CRK	195	199	PF00017	0.444
LIG_SH2_CRK	71	75	PF00017	0.378
LIG_SH2_NCK_1	227	231	PF00017	0.249
LIG_SH2_SRC	338	341	PF00017	0.366
LIG_SH2_STAT5	115	118	PF00017	0.402
LIG_SH2_STAT5	138	141	PF00017	0.455
LIG_SH2_STAT5	236	239	PF00017	0.458
LIG_SH2_STAT5	324	327	PF00017	0.411
LIG_SH2_STAT5	338	341	PF00017	0.276
LIG_SH3_3	148	154	PF00018	0.598
LIG_SH3_3	166	172	PF00018	0.291
LIG_SUMO_SIM_anti_2	288	297	PF11976	0.485
LIG_SUMO_SIM_anti_2	86	91	PF11976	0.455
LIG_UBA3_1	368	377	PF00899	0.455
LIG_WRC_WIRS_1	258	263	PF05994	0.444
MOD_CK1_1	21	27	PF00069	0.576
MOD_CK1_1	297	303	PF00069	0.468
MOD_CK2_1	293	299	PF00069	0.384
MOD_Cter_Amidation	101	104	PF01082	0.404
MOD_GlcNHglycan	140	143	PF01048	0.487
MOD_GlcNHglycan	296	299	PF01048	0.407
MOD_GlcNHglycan	342	345	PF01048	0.419
MOD_GSK3_1	105	112	PF00069	0.389
MOD_GSK3_1	293	300	PF00069	0.406
MOD_GSK3_1	346	353	PF00069	0.452
MOD_NEK2_1	105	110	PF00069	0.402
MOD_NEK2_1	146	151	PF00069	0.538
MOD_NEK2_1	160	165	PF00069	0.303
MOD_NEK2_1	18	23	PF00069	0.628
MOD_NEK2_1	292	297	PF00069	0.442
MOD_NEK2_1	346	351	PF00069	0.432
MOD_PIKK_1	93	99	PF00454	0.293
MOD_PK_1	3	9	PF00069	0.703
MOD_PKA_1	3	9	PF00069	0.667
MOD_PKA_2	146	152	PF00069	0.491
MOD_PKA_2	3	9	PF00069	0.588
MOD_Plk_1	274	280	PF00069	0.423
MOD_Plk_4	109	115	PF00069	0.351
MOD_Plk_4	231	237	PF00069	0.353
MOD_Plk_4	257	263	PF00069	0.375
MOD_Plk_4	3	9	PF00069	0.602
TRG_DiLeu_BaEn_1	288	293	PF01217	0.371
TRG_DiLeu_BaEn_1	49	54	PF01217	0.249
TRG_ENDOCYTIC_2	195	198	PF00928	0.392
TRG_ENDOCYTIC_2	71	74	PF00928	0.378
TRG_ER_diArg_1	3	5	PF00400	0.667
TRG_ER_diArg_1	363	365	PF00400	0.387
TRG_Pf-PMV_PEXEL_1	253	257	PF00026	0.347

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1HVU7	Leptomonas seymouri	50%	100%
A0A0S4IKM8	Bodo saltans	23%	98%
A0A0S4JJE1	Bodo saltans	46%	100%
A0A1X0NSF7	Trypanosomatidae	47%	99%
A0A3S7WZP2	Leishmania donovani	94%	100%
A0A422N2J8	Trypanosoma rangeli	49%	100%
A4HES1	Leishmania braziliensis	52%	99%
A4I1Z8	Leishmania infantum	94%	100%
C9ZS16	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	43%	100%
E9AY45	Leishmania mexicana (strain MHOM/GT/2001/U1103)	88%	99%
O13811	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	38%	100%
O22263	Arabidopsis thaliana	40%	100%
P38660	Mesocricetus auratus	36%	86%
P38661	Medicago sativa	40%	100%
Q00216	Aspergillus niger	42%	100%
Q11067	Caenorhabditis elegans	31%	86%
Q15084	Homo sapiens	35%	86%
Q5R6T1	Pongo abelii	35%	86%
Q63081	Rattus norvegicus	35%	86%
Q75M08	Oryza sativa subsp. japonica	41%	100%
Q86IA3	Dictyostelium discoideum	34%	100%
Q92249	Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)	39%	100%
Q922R8	Mus musculus	35%	86%
Q942L2	Oryza sativa subsp. japonica	38%	100%
Q9MAU6	Arabidopsis thaliana	31%	84%
Q9V438	Drosophila melanogaster	35%	87%
Q9VYV3	Drosophila melanogaster	27%	91%
V5DR98	Trypanosoma cruzi	45%	100%