Putative sphingosine kinase A, B

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Sphingosine kinase

Species:

Leishmania major

UniProt:

Q4Q9C3_LEIMA

TriTrypDb:

LmjF.26.0710 , LMJLV39_260012500 , LMJSD75_260011000

Length:

935

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 9
NetGPI	no	yes: 0, no: 9

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	2
GO:0016020	membrane	2	2
GO:0043226	organelle	2	2
GO:0043227	membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043231	intracellular membrane-bounded organelle	4	2
GO:0110165	cellular anatomical entity	1	2

Expansion

Sequence features

Q4Q9C3

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4Q9C3

Function

Biological processes
Term	Name	Level	Count
GO:0006066	alcohol metabolic process	3	2
GO:0006629	lipid metabolic process	3	2
GO:0006643	membrane lipid metabolic process	4	2
GO:0006665	sphingolipid metabolic process	4	2
GO:0006670	sphingosine metabolic process	6	2
GO:0006793	phosphorus metabolic process	3	5
GO:0006796	phosphate-containing compound metabolic process	4	5
GO:0006807	nitrogen compound metabolic process	2	2
GO:0008152	metabolic process	1	5
GO:0008610	lipid biosynthetic process	4	2
GO:0009058	biosynthetic process	2	2
GO:0009987	cellular process	1	5
GO:0016310	phosphorylation	5	5
GO:0019751	polyol metabolic process	4	2
GO:0030148	sphingolipid biosynthetic process	5	2
GO:0034311	diol metabolic process	5	2
GO:0034312	diol biosynthetic process	6	2
GO:0034641	cellular nitrogen compound metabolic process	3	2
GO:0044237	cellular metabolic process	2	5
GO:0044238	primary metabolic process	2	2
GO:0044249	cellular biosynthetic process	3	2
GO:0044255	cellular lipid metabolic process	3	2
GO:0044271	cellular nitrogen compound biosynthetic process	4	2
GO:0044281	small molecule metabolic process	2	2
GO:0044283	small molecule biosynthetic process	3	2
GO:0046165	alcohol biosynthetic process	4	2
GO:0046173	polyol biosynthetic process	5	2
GO:0046467	membrane lipid biosynthetic process	4	2
GO:0046512	sphingosine biosynthetic process	5	2
GO:0046519	sphingoid metabolic process	5	2
GO:0046520	sphingoid biosynthetic process	6	2
GO:0071704	organic substance metabolic process	2	2
GO:1901564	organonitrogen compound metabolic process	3	2
GO:1901566	organonitrogen compound biosynthetic process	4	2
GO:1901576	organic substance biosynthetic process	3	2
GO:1901615	organic hydroxy compound metabolic process	3	2
GO:1901617	organic hydroxy compound biosynthetic process	4	2

Molecular functions
Term	Name	Level	Count
GO:0001727	lipid kinase activity	5	5
GO:0003824	catalytic activity	1	10
GO:0008481	sphinganine kinase activity	5	5
GO:0016301	kinase activity	4	10
GO:0016740	transferase activity	2	10
GO:0016772	transferase activity, transferring phosphorus-containing groups	3	10
GO:0016773	phosphotransferase activity, alcohol group as acceptor	4	6
GO:0017050	D-erythro-sphingosine kinase activity	5	10
GO:0003951	NAD+ kinase activity	5	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	908	912	PF00656	0.289
CLV_NRD_NRD_1	383	385	PF00675	0.307
CLV_NRD_NRD_1	423	425	PF00675	0.262
CLV_NRD_NRD_1	595	597	PF00675	0.452
CLV_PCSK_KEX2_1	383	385	PF00082	0.307
CLV_PCSK_KEX2_1	423	425	PF00082	0.261
CLV_PCSK_KEX2_1	497	499	PF00082	0.635
CLV_PCSK_KEX2_1	595	597	PF00082	0.392
CLV_PCSK_KEX2_1	707	709	PF00082	0.352
CLV_PCSK_PC1ET2_1	497	499	PF00082	0.635
CLV_PCSK_PC1ET2_1	707	709	PF00082	0.352
CLV_PCSK_PC7_1	591	597	PF00082	0.370
CLV_PCSK_SKI1_1	189	193	PF00082	0.448
CLV_PCSK_SKI1_1	223	227	PF00082	0.610
CLV_PCSK_SKI1_1	234	238	PF00082	0.544
CLV_PCSK_SKI1_1	367	371	PF00082	0.325
CLV_PCSK_SKI1_1	425	429	PF00082	0.427
CLV_PCSK_SKI1_1	433	437	PF00082	0.482
CLV_PCSK_SKI1_1	630	634	PF00082	0.468
CLV_PCSK_SKI1_1	665	669	PF00082	0.318
CLV_PCSK_SKI1_1	704	708	PF00082	0.456
CLV_PCSK_SKI1_1	710	714	PF00082	0.401
DEG_APCC_DBOX_1	422	430	PF00400	0.442
DEG_APCC_KENBOX_2	306	310	PF00400	0.459
DEG_SCF_FBW7_1	270	277	PF00400	0.561
DEG_SCF_FBW7_1	776	783	PF00400	0.596
DEG_SCF_FBW7_2	506	511	PF00400	0.399
DEG_SCF_TRCP1_1	110	115	PF00400	0.575
DEG_SPOP_SBC_1	120	124	PF00917	0.583
DOC_CDC14_PxL_1	621	629	PF14671	0.463
DOC_CKS1_1	271	276	PF01111	0.509
DOC_CKS1_1	63	68	PF01111	0.512
DOC_CYCLIN_RxL_1	364	373	PF00134	0.360
DOC_CYCLIN_yCln2_LP_2	30	33	PF00134	0.557
DOC_CYCLIN_yCln2_LP_2	842	848	PF00134	0.377
DOC_MAPK_DCC_7	813	822	PF00069	0.340
DOC_MAPK_gen_1	187	196	PF00069	0.460
DOC_MAPK_gen_1	340	348	PF00069	0.344
DOC_MAPK_gen_1	364	372	PF00069	0.370
DOC_MAPK_gen_1	813	822	PF00069	0.340
DOC_MAPK_gen_1	867	876	PF00069	0.267
DOC_MAPK_gen_1	895	903	PF00069	0.362
DOC_MAPK_gen_1	921	931	PF00069	0.377
DOC_MAPK_HePTP_8	810	822	PF00069	0.337
DOC_MAPK_MEF2A_6	189	198	PF00069	0.466
DOC_MAPK_MEF2A_6	364	372	PF00069	0.394
DOC_MAPK_MEF2A_6	665	673	PF00069	0.418
DOC_MAPK_MEF2A_6	813	822	PF00069	0.340
DOC_MAPK_MEF2A_6	895	903	PF00069	0.446
DOC_PP1_RVXF_1	187	193	PF00149	0.320
DOC_PP1_RVXF_1	221	227	PF00149	0.648
DOC_PP1_RVXF_1	311	318	PF00149	0.393
DOC_PP1_RVXF_1	663	669	PF00149	0.339
DOC_PP2B_LxvP_1	30	33	PF13499	0.557
DOC_PP2B_LxvP_1	842	845	PF13499	0.377
DOC_PP4_FxxP_1	192	195	PF00568	0.284
DOC_PP4_FxxP_1	71	74	PF00568	0.511
DOC_SPAK_OSR1_1	693	697	PF12202	0.393
DOC_USP7_MATH_1	106	110	PF00917	0.726
DOC_USP7_MATH_1	118	122	PF00917	0.625
DOC_USP7_MATH_1	274	278	PF00917	0.655
DOC_USP7_MATH_1	291	295	PF00917	0.615
DOC_USP7_MATH_1	398	402	PF00917	0.272
DOC_USP7_MATH_1	437	441	PF00917	0.537
DOC_USP7_MATH_1	540	544	PF00917	0.412
DOC_USP7_MATH_1	667	671	PF00917	0.299
DOC_USP7_MATH_1	717	721	PF00917	0.471
DOC_USP7_MATH_1	780	784	PF00917	0.746
DOC_USP7_MATH_1	847	851	PF00917	0.302
DOC_USP7_MATH_1	916	920	PF00917	0.377
DOC_USP7_UBL2_3	724	728	PF12436	0.478
DOC_WW_Pin1_4	121	126	PF00397	0.667
DOC_WW_Pin1_4	270	275	PF00397	0.603
DOC_WW_Pin1_4	33	38	PF00397	0.568
DOC_WW_Pin1_4	445	450	PF00397	0.586
DOC_WW_Pin1_4	480	485	PF00397	0.639
DOC_WW_Pin1_4	504	509	PF00397	0.611
DOC_WW_Pin1_4	576	581	PF00397	0.449
DOC_WW_Pin1_4	6	11	PF00397	0.578
DOC_WW_Pin1_4	62	67	PF00397	0.715
DOC_WW_Pin1_4	636	641	PF00397	0.539
DOC_WW_Pin1_4	76	81	PF00397	0.619
DOC_WW_Pin1_4	776	781	PF00397	0.684
LIG_14-3-3_CanoR_1	164	169	PF00244	0.523
LIG_14-3-3_CanoR_1	231	237	PF00244	0.715
LIG_14-3-3_CanoR_1	374	378	PF00244	0.318
LIG_14-3-3_CanoR_1	430	436	PF00244	0.485
LIG_14-3-3_CanoR_1	589	597	PF00244	0.514
LIG_14-3-3_CanoR_1	630	638	PF00244	0.496
LIG_14-3-3_CanoR_1	784	790	PF00244	0.589
LIG_APCC_ABBA_1	796	801	PF00400	0.477
LIG_BIR_III_4	54	58	PF00653	0.586
LIG_BRCT_BRCA1_1	484	488	PF00533	0.467
LIG_BRCT_BRCA1_1	60	64	PF00533	0.529
LIG_BRCT_BRCA1_1	669	673	PF00533	0.308
LIG_BRCT_BRCA1_1	927	931	PF00533	0.430
LIG_eIF4E_1	799	805	PF01652	0.462
LIG_FHA_1	186	192	PF00498	0.580
LIG_FHA_1	219	225	PF00498	0.720
LIG_FHA_1	231	237	PF00498	0.475
LIG_FHA_1	25	31	PF00498	0.603
LIG_FHA_1	257	263	PF00498	0.679
LIG_FHA_1	434	440	PF00498	0.544
LIG_FHA_1	637	643	PF00498	0.554
LIG_FHA_1	90	96	PF00498	0.621
LIG_FHA_2	202	208	PF00498	0.363
LIG_FHA_2	511	517	PF00498	0.390
LIG_FHA_2	577	583	PF00498	0.497
LIG_FHA_2	631	637	PF00498	0.522
LIG_FHA_2	743	749	PF00498	0.396
LIG_GSK3_LRP6_1	270	275	PF00069	0.488
LIG_Integrin_RGD_1	823	825	PF01839	0.368
LIG_LIR_Gen_1	485	495	PF02991	0.730
LIG_LIR_Gen_1	855	864	PF02991	0.346
LIG_LIR_Nem_3	485	491	PF02991	0.714
LIG_LIR_Nem_3	603	607	PF02991	0.453
LIG_LIR_Nem_3	855	861	PF02991	0.346
LIG_LIR_Nem_3	889	893	PF02991	0.377
LIG_MYND_1	625	629	PF01753	0.474
LIG_PCNA_yPIPBox_3	327	340	PF02747	0.353
LIG_PTB_Apo_2	688	695	PF02174	0.352
LIG_SH2_CRK	700	704	PF00017	0.422
LIG_SH2_PTP2	756	759	PF00017	0.318
LIG_SH2_STAT3	301	304	PF00017	0.482
LIG_SH2_STAT3	799	802	PF00017	0.370
LIG_SH2_STAT5	170	173	PF00017	0.482
LIG_SH2_STAT5	298	301	PF00017	0.319
LIG_SH2_STAT5	391	394	PF00017	0.289
LIG_SH2_STAT5	715	718	PF00017	0.370
LIG_SH2_STAT5	756	759	PF00017	0.438
LIG_SH2_STAT5	799	802	PF00017	0.476
LIG_SH2_STAT5	858	861	PF00017	0.377
LIG_SH2_STAT5	893	896	PF00017	0.267
LIG_SH3_2	625	630	PF14604	0.462
LIG_SH3_3	268	274	PF00018	0.716
LIG_SH3_3	443	449	PF00018	0.499
LIG_SH3_3	478	484	PF00018	0.565
LIG_SH3_3	622	628	PF00018	0.466
LIG_SH3_3	77	83	PF00018	0.667
LIG_SH3_3	792	798	PF00018	0.375
LIG_SUMO_SIM_anti_2	263	268	PF11976	0.495
LIG_SUMO_SIM_anti_2	550	555	PF11976	0.359
LIG_SUMO_SIM_anti_2	572	577	PF11976	0.407
LIG_SUMO_SIM_par_1	552	558	PF11976	0.449
LIG_TYR_ITIM	856	861	PF00017	0.355
LIG_UBA3_1	345	352	PF00899	0.318
LIG_UBA3_1	702	710	PF00899	0.396
LIG_UBA3_1	712	721	PF00899	0.299
LIG_WW_3	627	631	PF00397	0.500
MOD_CDK_SPK_2	62	67	PF00069	0.515
MOD_CDK_SPxxK_3	62	69	PF00069	0.532
MOD_CDK_SPxxK_3	636	643	PF00069	0.503
MOD_CK1_1	121	127	PF00069	0.657
MOD_CK1_1	173	179	PF00069	0.421
MOD_CK1_1	239	245	PF00069	0.719
MOD_CK1_1	24	30	PF00069	0.568
MOD_CK1_1	256	262	PF00069	0.501
MOD_CK1_1	3	9	PF00069	0.599
MOD_CK1_1	311	317	PF00069	0.419
MOD_CK1_1	350	356	PF00069	0.261
MOD_CK1_1	373	379	PF00069	0.377
MOD_CK1_1	42	48	PF00069	0.480
MOD_CK1_1	445	451	PF00069	0.596
MOD_CK1_1	480	486	PF00069	0.717
MOD_CK1_1	672	678	PF00069	0.349
MOD_CK1_1	76	82	PF00069	0.638
MOD_CK1_1	765	771	PF00069	0.590
MOD_CK1_1	783	789	PF00069	0.593
MOD_CK1_1	98	104	PF00069	0.689
MOD_CK2_1	201	207	PF00069	0.374
MOD_CK2_1	42	48	PF00069	0.763
MOD_CK2_1	510	516	PF00069	0.393
MOD_CK2_1	552	558	PF00069	0.447
MOD_CK2_1	576	582	PF00069	0.453
MOD_CK2_1	742	748	PF00069	0.543
MOD_CK2_1	788	794	PF00069	0.514
MOD_CK2_1	907	913	PF00069	0.284
MOD_GlcNHglycan	103	106	PF01048	0.650
MOD_GlcNHglycan	110	113	PF01048	0.592
MOD_GlcNHglycan	228	231	PF01048	0.557
MOD_GlcNHglycan	241	244	PF01048	0.686
MOD_GlcNHglycan	319	322	PF01048	0.581
MOD_GlcNHglycan	349	352	PF01048	0.286
MOD_GlcNHglycan	44	47	PF01048	0.520
MOD_GlcNHglycan	466	469	PF01048	0.694
MOD_GlcNHglycan	508	511	PF01048	0.600
MOD_GlcNHglycan	542	545	PF01048	0.378
MOD_GlcNHglycan	60	63	PF01048	0.518
MOD_GlcNHglycan	674	677	PF01048	0.257
MOD_GlcNHglycan	790	793	PF01048	0.533
MOD_GlcNHglycan	881	884	PF01048	0.341
MOD_GlcNHglycan	97	100	PF01048	0.718
MOD_GSK3_1	106	113	PF00069	0.662
MOD_GSK3_1	116	123	PF00069	0.560
MOD_GSK3_1	13	20	PF00069	0.686
MOD_GSK3_1	173	180	PF00069	0.434
MOD_GSK3_1	181	188	PF00069	0.481
MOD_GSK3_1	2	9	PF00069	0.684
MOD_GSK3_1	21	28	PF00069	0.557
MOD_GSK3_1	226	233	PF00069	0.520
MOD_GSK3_1	256	263	PF00069	0.535
MOD_GSK3_1	270	277	PF00069	0.543
MOD_GSK3_1	287	294	PF00069	0.519
MOD_GSK3_1	433	440	PF00069	0.468
MOD_GSK3_1	445	452	PF00069	0.506
MOD_GSK3_1	506	513	PF00069	0.562
MOD_GSK3_1	58	65	PF00069	0.575
MOD_GSK3_1	742	749	PF00069	0.412
MOD_GSK3_1	776	783	PF00069	0.707
MOD_GSK3_1	79	86	PF00069	0.549
MOD_GSK3_1	916	923	PF00069	0.340
MOD_N-GLC_1	20	25	PF02516	0.518
MOD_N-GLC_1	437	442	PF02516	0.498
MOD_N-GLC_1	576	581	PF02516	0.436
MOD_N-GLC_1	6	11	PF02516	0.580
MOD_N-GLC_1	780	785	PF02516	0.716
MOD_N-GLC_1	95	100	PF02516	0.767
MOD_NEK2_1	177	182	PF00069	0.508
MOD_NEK2_1	226	231	PF00069	0.593
MOD_NEK2_1	236	241	PF00069	0.656
MOD_NEK2_1	253	258	PF00069	0.607
MOD_NEK2_1	262	267	PF00069	0.569
MOD_NEK2_1	317	322	PF00069	0.495
MOD_NEK2_1	370	375	PF00069	0.303
MOD_NEK2_1	39	44	PF00069	0.593
MOD_NEK2_1	464	469	PF00069	0.482
MOD_NEK2_1	535	540	PF00069	0.299
MOD_NEK2_1	669	674	PF00069	0.310
MOD_NEK2_1	746	751	PF00069	0.586
MOD_NEK2_1	925	930	PF00069	0.440
MOD_NEK2_2	449	454	PF00069	0.518
MOD_NEK2_2	682	687	PF00069	0.418
MOD_NEK2_2	742	747	PF00069	0.500
MOD_NEK2_2	818	823	PF00069	0.448
MOD_NEK2_2	83	88	PF00069	0.520
MOD_NEK2_2	916	921	PF00069	0.377
MOD_OFUCOSY	744	750	PF10250	0.470
MOD_PKA_2	17	23	PF00069	0.606
MOD_PKA_2	173	179	PF00069	0.422
MOD_PKA_2	230	236	PF00069	0.645
MOD_PKA_2	373	379	PF00069	0.325
MOD_PKA_2	590	596	PF00069	0.414
MOD_PKA_2	746	752	PF00069	0.561
MOD_PKA_2	783	789	PF00069	0.624
MOD_PKA_2	920	926	PF00069	0.377
MOD_PKB_1	589	597	PF00069	0.318
MOD_Plk_1	218	224	PF00069	0.558
MOD_Plk_1	449	455	PF00069	0.672
MOD_Plk_1	477	483	PF00069	0.762
MOD_Plk_1	602	608	PF00069	0.387
MOD_Plk_1	616	622	PF00069	0.426
MOD_Plk_2-3	552	558	PF00069	0.379
MOD_Plk_4	26	32	PF00069	0.597
MOD_Plk_4	262	268	PF00069	0.645
MOD_Plk_4	329	335	PF00069	0.385
MOD_Plk_4	400	406	PF00069	0.377
MOD_Plk_4	442	448	PF00069	0.699
MOD_Plk_4	559	565	PF00069	0.333
MOD_ProDKin_1	121	127	PF00069	0.668
MOD_ProDKin_1	270	276	PF00069	0.604
MOD_ProDKin_1	33	39	PF00069	0.572
MOD_ProDKin_1	445	451	PF00069	0.587
MOD_ProDKin_1	480	486	PF00069	0.640
MOD_ProDKin_1	504	510	PF00069	0.595
MOD_ProDKin_1	576	582	PF00069	0.453
MOD_ProDKin_1	6	12	PF00069	0.575
MOD_ProDKin_1	62	68	PF00069	0.713
MOD_ProDKin_1	636	642	PF00069	0.527
MOD_ProDKin_1	76	82	PF00069	0.620
MOD_ProDKin_1	776	782	PF00069	0.683
MOD_SUMO_for_1	812	815	PF00179	0.351
MOD_SUMO_rev_2	603	613	PF00179	0.379
TRG_DiLeu_BaEn_2	871	877	PF01217	0.218
TRG_DiLeu_BaLyEn_6	365	370	PF01217	0.323
TRG_ENDOCYTIC_2	700	703	PF00928	0.340
TRG_ENDOCYTIC_2	709	712	PF00928	0.322
TRG_ENDOCYTIC_2	756	759	PF00928	0.318
TRG_ENDOCYTIC_2	858	861	PF00928	0.349
TRG_ER_diArg_1	589	592	PF00400	0.580
TRG_ER_diArg_1	595	597	PF00400	0.374
TRG_ER_diArg_1	618	621	PF00400	0.497
TRG_NLS_MonoExtN_4	704	711	PF00514	0.338
TRG_Pf-PMV_PEXEL_1	313	318	PF00026	0.392
TRG_Pf-PMV_PEXEL_1	522	526	PF00026	0.343

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N0P530	Leptomonas seymouri	55%	96%
A0A1X0NSV1	Trypanosomatidae	30%	100%
A0A3Q8IGX7	Leishmania donovani	88%	100%
A0A422NN92	Trypanosoma rangeli	31%	100%
A4HES6	Leishmania braziliensis	72%	100%
A4I203	Leishmania infantum	88%	100%
C9ZS12	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	30%	100%
E9AY50	Leishmania mexicana (strain MHOM/GT/2001/U1103)	80%	100%