Putative DNA ligase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Putative DNA ligase

Gene product:

mitochondrial DNA ligase homolog, putative

Species:

Leishmania major

UniProt:

Q4Q959_LEIMA

TriTrypDb:

LmjF.26.1350 , LMJLV39_260019200 , LMJSD75_260017600

Length:

433

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 22
NetGPI	no	yes: 0, no: 22

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	2
GO:0110165	cellular anatomical entity	1	3
GO:0005739	mitochondrion	5	1
GO:0020023	kinetoplast	2	1
GO:0043226	organelle	2	1
GO:0043227	membrane-bounded organelle	3	1
GO:0043229	intracellular organelle	3	1
GO:0043231	intracellular membrane-bounded organelle	4	1

Expansion

Sequence features

Q4Q959

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4Q959

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	23
GO:0006259	DNA metabolic process	4	23
GO:0006281	DNA repair	5	23
GO:0006310	DNA recombination	5	23
GO:0006725	cellular aromatic compound metabolic process	3	23
GO:0006807	nitrogen compound metabolic process	2	23
GO:0006950	response to stress	2	23
GO:0006974	DNA damage response	4	23
GO:0008152	metabolic process	1	23
GO:0009987	cellular process	1	23
GO:0033554	cellular response to stress	3	23
GO:0034641	cellular nitrogen compound metabolic process	3	23
GO:0043170	macromolecule metabolic process	3	23
GO:0044237	cellular metabolic process	2	23
GO:0044238	primary metabolic process	2	23
GO:0044260	obsolete cellular macromolecule metabolic process	3	23
GO:0046483	heterocycle metabolic process	3	23
GO:0050896	response to stimulus	1	23
GO:0051716	cellular response to stimulus	2	23
GO:0071704	organic substance metabolic process	2	23
GO:0090304	nucleic acid metabolic process	4	23
GO:1901360	organic cyclic compound metabolic process	3	23

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	23
GO:0003824	catalytic activity	1	23
GO:0003909	DNA ligase activity	4	23
GO:0003910	DNA ligase (ATP) activity	5	23
GO:0005488	binding	1	23
GO:0005524	ATP binding	5	23
GO:0016874	ligase activity	2	23
GO:0016886	ligase activity, forming phosphoric ester bonds	3	23
GO:0017076	purine nucleotide binding	4	23
GO:0030554	adenyl nucleotide binding	5	23
GO:0032553	ribonucleotide binding	3	23
GO:0032555	purine ribonucleotide binding	4	23
GO:0032559	adenyl ribonucleotide binding	5	23
GO:0035639	purine ribonucleoside triphosphate binding	4	23
GO:0036094	small molecule binding	2	23
GO:0043167	ion binding	2	23
GO:0043168	anion binding	3	23
GO:0097159	organic cyclic compound binding	2	23
GO:0097367	carbohydrate derivative binding	2	23
GO:0140097	catalytic activity, acting on DNA	3	23
GO:0140640	catalytic activity, acting on a nucleic acid	2	23
GO:1901265	nucleoside phosphate binding	3	23
GO:1901363	heterocyclic compound binding	2	23

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	369	373	PF00656	0.446
CLV_NRD_NRD_1	200	202	PF00675	0.290
CLV_NRD_NRD_1	265	267	PF00675	0.322
CLV_NRD_NRD_1	386	388	PF00675	0.362
CLV_PCSK_KEX2_1	200	202	PF00082	0.309
CLV_PCSK_KEX2_1	204	206	PF00082	0.280
CLV_PCSK_KEX2_1	255	257	PF00082	0.342
CLV_PCSK_KEX2_1	331	333	PF00082	0.311
CLV_PCSK_KEX2_1	385	387	PF00082	0.346
CLV_PCSK_KEX2_1	429	431	PF00082	0.502
CLV_PCSK_PC1ET2_1	204	206	PF00082	0.407
CLV_PCSK_PC1ET2_1	255	257	PF00082	0.347
CLV_PCSK_PC1ET2_1	331	333	PF00082	0.322
CLV_PCSK_PC1ET2_1	385	387	PF00082	0.355
CLV_PCSK_PC1ET2_1	429	431	PF00082	0.483
CLV_PCSK_PC7_1	200	206	PF00082	0.193
CLV_PCSK_SKI1_1	124	128	PF00082	0.380
CLV_PCSK_SKI1_1	241	245	PF00082	0.327
CLV_PCSK_SKI1_1	360	364	PF00082	0.470
CLV_PCSK_SKI1_1	400	404	PF00082	0.367
CLV_PCSK_SKI1_1	81	85	PF00082	0.397
DEG_Nend_UBRbox_3	1	3	PF02207	0.460
DEG_SIAH_1	293	301	PF03145	0.409
DOC_CKS1_1	1	6	PF01111	0.338
DOC_CKS1_1	376	381	PF01111	0.291
DOC_MAPK_gen_1	48	55	PF00069	0.665
DOC_PP1_RVXF_1	145	152	PF00149	0.415
DOC_PP1_RVXF_1	346	353	PF00149	0.426
DOC_PP1_RVXF_1	79	86	PF00149	0.534
DOC_PP2B_LxvP_1	185	188	PF13499	0.338
DOC_PP4_FxxP_1	376	379	PF00568	0.440
DOC_PP4_FxxP_1	85	88	PF00568	0.527
DOC_USP7_MATH_1	380	384	PF00917	0.467
DOC_USP7_UBL2_3	381	385	PF12436	0.441
DOC_WW_Pin1_4	127	132	PF00397	0.329
DOC_WW_Pin1_4	375	380	PF00397	0.372
DOC_WW_Pin1_4	42	47	PF00397	0.579
DOC_WW_Pin1_4	5	10	PF00397	0.358
LIG_14-3-3_CanoR_1	18	26	PF00244	0.337
LIG_14-3-3_CanoR_1	271	277	PF00244	0.477
LIG_Actin_WH2_2	132	149	PF00022	0.252
LIG_Actin_WH2_2	346	362	PF00022	0.193
LIG_BRCT_BRCA1_1	234	238	PF00533	0.418
LIG_BRCT_BRCA1_1	311	315	PF00533	0.380
LIG_BRCT_BRCA1_1	81	85	PF00533	0.521
LIG_FHA_1	176	182	PF00498	0.389
LIG_FHA_1	277	283	PF00498	0.407
LIG_FHA_2	118	124	PF00498	0.310
LIG_FHA_2	367	373	PF00498	0.434
LIG_LIR_Apic_2	82	88	PF02991	0.515
LIG_LIR_Gen_1	189	199	PF02991	0.380
LIG_LIR_Gen_1	240	250	PF02991	0.346
LIG_LIR_Gen_1	25	34	PF02991	0.310
LIG_LIR_Gen_1	403	414	PF02991	0.360
LIG_LIR_Nem_3	189	195	PF02991	0.362
LIG_LIR_Nem_3	219	223	PF02991	0.299
LIG_LIR_Nem_3	240	245	PF02991	0.351
LIG_LIR_Nem_3	25	29	PF02991	0.310
LIG_LIR_Nem_3	312	318	PF02991	0.308
LIG_LIR_Nem_3	333	337	PF02991	0.352
LIG_LIR_Nem_3	344	350	PF02991	0.416
LIG_LIR_Nem_3	403	409	PF02991	0.362
LIG_MYND_1	167	171	PF01753	0.338
LIG_PCNA_PIPBox_1	214	223	PF02747	0.338
LIG_PCNA_yPIPBox_3	209	221	PF02747	0.349
LIG_PDZ_Class_3	428	433	PF00595	0.596
LIG_PDZ_Wminus1_1	431	433	PF00595	0.605
LIG_REV1ctd_RIR_1	149	158	PF16727	0.357
LIG_SH2_CRK	242	246	PF00017	0.322
LIG_SH2_CRK	291	295	PF00017	0.398
LIG_SH2_CRK	80	84	PF00017	0.420
LIG_SH2_SRC	327	330	PF00017	0.297
LIG_SH2_STAP1	227	231	PF00017	0.368
LIG_SH2_STAT3	227	230	PF00017	0.446
LIG_SH2_STAT5	220	223	PF00017	0.338
LIG_SH2_STAT5	260	263	PF00017	0.423
LIG_SH2_STAT5	375	378	PF00017	0.402
LIG_SH2_STAT5	397	400	PF00017	0.366
LIG_SH2_STAT5	401	404	PF00017	0.347
LIG_SH2_STAT5	69	72	PF00017	0.569
LIG_SH3_3	231	237	PF00018	0.327
LIG_SH3_3	320	326	PF00018	0.338
LIG_SH3_3	412	418	PF00018	0.329
LIG_SH3_3	67	73	PF00018	0.563
LIG_SUMO_SIM_par_1	176	182	PF11976	0.364
LIG_TYR_ITIM	404	409	PF00017	0.347
LIG_TYR_ITIM	78	83	PF00017	0.303
LIG_UBA3_1	169	175	PF00899	0.446
LIG_UBA3_1	319	324	PF00899	0.310
MOD_CDC14_SPxK_1	45	48	PF00782	0.385
MOD_CDK_SPK_2	127	132	PF00069	0.329
MOD_CDK_SPxK_1	375	381	PF00069	0.382
MOD_CDK_SPxK_1	42	48	PF00069	0.382
MOD_CDK_SPxxK_3	42	49	PF00069	0.389
MOD_CK1_1	232	238	PF00069	0.368
MOD_CK1_1	5	11	PF00069	0.367
MOD_CK2_1	111	117	PF00069	0.406
MOD_CK2_1	298	304	PF00069	0.354
MOD_Cter_Amidation	329	332	PF01082	0.322
MOD_GlcNHglycan	117	120	PF01048	0.270
MOD_GlcNHglycan	227	230	PF01048	0.369
MOD_GlcNHglycan	234	237	PF01048	0.405
MOD_GlcNHglycan	360	363	PF01048	0.416
MOD_GSK3_1	111	118	PF00069	0.433
MOD_GSK3_1	203	210	PF00069	0.348
MOD_GSK3_1	225	232	PF00069	0.322
MOD_GSK3_1	305	312	PF00069	0.371
MOD_N-GLC_1	5	10	PF02516	0.409
MOD_N-GLC_1	51	56	PF02516	0.631
MOD_NEK2_1	366	371	PF00069	0.462
MOD_NEK2_2	380	385	PF00069	0.482
MOD_PIKK_1	309	315	PF00454	0.357
MOD_PIKK_1	408	414	PF00454	0.354
MOD_PKA_1	255	261	PF00069	0.357
MOD_PKA_2	17	23	PF00069	0.321
MOD_PKA_2	255	261	PF00069	0.357
MOD_PKA_2	270	276	PF00069	0.357
MOD_PKA_2	423	429	PF00069	0.550
MOD_Plk_1	51	57	PF00069	0.665
MOD_Plk_2-3	207	213	PF00069	0.387
MOD_Plk_4	175	181	PF00069	0.380
MOD_ProDKin_1	127	133	PF00069	0.329
MOD_ProDKin_1	375	381	PF00069	0.372
MOD_ProDKin_1	42	48	PF00069	0.585
MOD_ProDKin_1	5	11	PF00069	0.358
MOD_SUMO_rev_2	206	211	PF00179	0.431
MOD_SUMO_rev_2	344	350	PF00179	0.517
TRG_DiLeu_BaEn_1	240	245	PF01217	0.322
TRG_DiLeu_BaLyEn_6	213	218	PF01217	0.446
TRG_ENDOCYTIC_2	220	223	PF00928	0.329
TRG_ENDOCYTIC_2	242	245	PF00928	0.347
TRG_ENDOCYTIC_2	339	342	PF00928	0.414
TRG_ENDOCYTIC_2	401	404	PF00928	0.340
TRG_ENDOCYTIC_2	406	409	PF00928	0.335
TRG_ENDOCYTIC_2	80	83	PF00928	0.426
TRG_ER_diArg_1	199	201	PF00400	0.318
TRG_ER_diArg_1	386	388	PF00400	0.372
TRG_NLS_Bipartite_1	255	270	PF00514	0.401

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PAX3	Leptomonas seymouri	77%	87%
A0A0S4IPC3	Bodo saltans	26%	100%
A0A0S4KIC5	Bodo saltans	34%	76%
A0A0S4KP75	Bodo saltans	34%	95%
A0A1X0NS61	Trypanosomatidae	32%	83%
A0A1X0NSF5	Trypanosomatidae	59%	83%
A0A3Q8IDN6	Leishmania donovani	96%	100%
A0A3Q8IDV4	Leishmania donovani	25%	69%
A0A3S5IR98	Trypanosoma rangeli	35%	89%
A0A422NIS8	Trypanosoma rangeli	56%	100%
A4HEY9	Leishmania braziliensis	26%	68%
A4HEZ0	Leishmania braziliensis	88%	91%
A4I263	Leishmania infantum	25%	69%
A4I264	Leishmania infantum	96%	100%
C9ZRT4	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	55%	85%
C9ZRT5	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	32%	90%
E9AYB3	Leishmania mexicana (strain MHOM/GT/2001/U1103)	30%	69%
E9AYB4	Leishmania mexicana (strain MHOM/GT/2001/U1103)	93%	100%
Q4Q960	Leishmania major	30%	69%
V5AUB9	Trypanosoma cruzi	60%	83%
V5DAB4	Trypanosoma cruzi	34%	89%