G domain-containing protein

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

ADP-ribosylation factor family, putative

Species:

Leishmania major

UniProt:

Q4Q8X3_LEIMA

TriTrypDb:

LmjF.26.2190 , LMJLV39_260028400 , LMJSD75_260026800

Length:

404

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 7
NetGPI	no	yes: 0, no: 7

Cellular components
Term	Name	Level	Count
GO:0005737	cytoplasm	2	2
GO:0005794	Golgi apparatus	5	2
GO:0043226	organelle	2	2
GO:0043227	membrane-bounded organelle	3	2
GO:0043229	intracellular organelle	3	2
GO:0043231	intracellular membrane-bounded organelle	4	2
GO:0110165	cellular anatomical entity	1	2

Expansion

Sequence features

Q4Q8X3

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4Q8X3

Function

Biological processes
Term	Name	Level	Count
GO:0006810	transport	3	2
GO:0006886	intracellular protein transport	4	2
GO:0006892	post-Golgi vesicle-mediated transport	6	2
GO:0006893	Golgi to plasma membrane transport	5	2
GO:0008104	protein localization	4	2
GO:0009987	cellular process	1	2
GO:0015031	protein transport	4	2
GO:0016192	vesicle-mediated transport	4	2
GO:0033036	macromolecule localization	2	2
GO:0033365	protein localization to organelle	5	2
GO:0034067	protein localization to Golgi apparatus	6	2
GO:0043001	Golgi to plasma membrane protein transport	5	2
GO:0045184	establishment of protein localization	3	2
GO:0046907	intracellular transport	3	2
GO:0048193	Golgi vesicle transport	5	2
GO:0051179	localization	1	2
GO:0051234	establishment of localization	2	2
GO:0051641	cellular localization	2	2
GO:0051649	establishment of localization in cell	3	2
GO:0051668	localization within membrane	3	2
GO:0061951	establishment of protein localization to plasma membrane	5	2
GO:0070727	cellular macromolecule localization	3	2
GO:0071702	organic substance transport	4	2
GO:0071705	nitrogen compound transport	4	2
GO:0072657	protein localization to membrane	4	2
GO:0072659	protein localization to plasma membrane	5	2
GO:0090150	establishment of protein localization to membrane	4	2
GO:0098876	vesicle-mediated transport to the plasma membrane	4	2
GO:1990778	protein localization to cell periphery	5	2

Molecular functions
Term	Name	Level	Count
GO:0000166	nucleotide binding	3	8
GO:0003824	catalytic activity	1	8
GO:0003924	GTPase activity	7	8
GO:0005488	binding	1	8
GO:0005525	GTP binding	5	8
GO:0016462	pyrophosphatase activity	5	8
GO:0016787	hydrolase activity	2	8
GO:0016817	hydrolase activity, acting on acid anhydrides	3	8
GO:0016818	hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides	4	8
GO:0017076	purine nucleotide binding	4	8
GO:0017111	ribonucleoside triphosphate phosphatase activity	6	8
GO:0019001	guanyl nucleotide binding	5	8
GO:0032553	ribonucleotide binding	3	8
GO:0032555	purine ribonucleotide binding	4	8
GO:0032561	guanyl ribonucleotide binding	5	8
GO:0035639	purine ribonucleoside triphosphate binding	4	8
GO:0036094	small molecule binding	2	8
GO:0043167	ion binding	2	8
GO:0043168	anion binding	3	8
GO:0097159	organic cyclic compound binding	2	8
GO:0097367	carbohydrate derivative binding	2	8
GO:1901265	nucleoside phosphate binding	3	8
GO:1901363	heterocyclic compound binding	2	8

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	375	379	PF00656	0.420
CLV_MEL_PAP_1	121	127	PF00089	0.440
CLV_MEL_PAP_1	355	361	PF00089	0.597
CLV_NRD_NRD_1	347	349	PF00675	0.613
CLV_NRD_NRD_1	85	87	PF00675	0.714
CLV_PCSK_KEX2_1	108	110	PF00082	0.537
CLV_PCSK_KEX2_1	347	349	PF00082	0.613
CLV_PCSK_KEX2_1	85	87	PF00082	0.677
CLV_PCSK_PC1ET2_1	108	110	PF00082	0.489
CLV_PCSK_SKI1_1	177	181	PF00082	0.439
CLV_PCSK_SKI1_1	248	252	PF00082	0.418
CLV_PCSK_SKI1_1	65	69	PF00082	0.602
DEG_APCC_DBOX_1	176	184	PF00400	0.360
DEG_APCC_DBOX_1	247	255	PF00400	0.444
DEG_Nend_Nbox_1	1	3	PF02207	0.493
DOC_CDC14_PxL_1	260	268	PF14671	0.369
DOC_CKS1_1	13	18	PF01111	0.499
DOC_CKS1_1	40	45	PF01111	0.490
DOC_CKS1_1	78	83	PF01111	0.767
DOC_CKS1_1	94	99	PF01111	0.408
DOC_CYCLIN_yCln2_LP_2	255	261	PF00134	0.476
DOC_MAPK_gen_1	108	116	PF00069	0.488
DOC_MAPK_gen_1	136	142	PF00069	0.581
DOC_MAPK_MEF2A_6	108	116	PF00069	0.488
DOC_PP4_FxxP_1	125	128	PF00568	0.467
DOC_PP4_FxxP_1	13	16	PF00568	0.527
DOC_PP4_FxxP_1	213	216	PF00568	0.231
DOC_USP7_MATH_1	144	148	PF00917	0.551
DOC_USP7_MATH_1	218	222	PF00917	0.641
DOC_USP7_MATH_1	28	32	PF00917	0.422
DOC_USP7_MATH_1	55	59	PF00917	0.569
DOC_WW_Pin1_4	12	17	PF00397	0.521
DOC_WW_Pin1_4	123	128	PF00397	0.429
DOC_WW_Pin1_4	216	221	PF00397	0.592
DOC_WW_Pin1_4	336	341	PF00397	0.776
DOC_WW_Pin1_4	39	44	PF00397	0.572
DOC_WW_Pin1_4	77	82	PF00397	0.765
DOC_WW_Pin1_4	93	98	PF00397	0.422
LIG_14-3-3_CanoR_1	347	357	PF00244	0.521
LIG_14-3-3_CanoR_1	57	67	PF00244	0.619
LIG_Actin_WH2_2	379	397	PF00022	0.312
LIG_FHA_1	111	117	PF00498	0.415
LIG_FHA_1	174	180	PF00498	0.405
LIG_FHA_1	270	276	PF00498	0.474
LIG_FHA_1	295	301	PF00498	0.485
LIG_FHA_1	40	46	PF00498	0.417
LIG_FHA_1	68	74	PF00498	0.724
LIG_FHA_2	234	240	PF00498	0.535
LIG_FHA_2	30	36	PF00498	0.349
LIG_FHA_2	348	354	PF00498	0.667
LIG_GBD_Chelix_1	203	211	PF00786	0.376
LIG_LIR_Apic_2	11	16	PF02991	0.533
LIG_LIR_Apic_2	210	216	PF02991	0.231
LIG_LIR_Gen_1	17	23	PF02991	0.416
LIG_LIR_Gen_1	6	16	PF02991	0.528
LIG_LIR_Nem_3	15	21	PF02991	0.472
LIG_LIR_Nem_3	191	196	PF02991	0.360
LIG_LIR_Nem_3	295	301	PF02991	0.576
LIG_LIR_Nem_3	362	367	PF02991	0.547
LIG_LIR_Nem_3	6	12	PF02991	0.559
LIG_NRBOX	250	256	PF00104	0.447
LIG_PCNA_PIPBox_1	186	195	PF02747	0.360
LIG_PCNA_yPIPBox_3	181	193	PF02747	0.360
LIG_PDZ_Class_1	399	404	PF00595	0.543
LIG_Pex14_1	193	197	PF04695	0.300
LIG_PTB_Apo_2	7	14	PF02174	0.538
LIG_SH2_CRK	118	122	PF00017	0.458
LIG_SH2_CRK	18	22	PF00017	0.347
LIG_SH2_CRK	9	13	PF00017	0.481
LIG_SH2_NCK_1	118	122	PF00017	0.458
LIG_SH2_STAP1	157	161	PF00017	0.532
LIG_SH2_STAT3	299	302	PF00017	0.591
LIG_SH2_STAT5	205	208	PF00017	0.360
LIG_SH2_STAT5	299	302	PF00017	0.521
LIG_SH2_STAT5	41	44	PF00017	0.482
LIG_SH3_1	88	94	PF00018	0.632
LIG_SH3_3	146	152	PF00018	0.608
LIG_SH3_3	217	223	PF00018	0.645
LIG_SH3_3	75	81	PF00018	0.754
LIG_SH3_3	88	94	PF00018	0.645
LIG_SUMO_SIM_anti_2	130	136	PF11976	0.588
LIG_SUMO_SIM_par_1	263	269	PF11976	0.372
LIG_SUMO_SIM_par_1	370	375	PF11976	0.423
LIG_TRAF2_1	236	239	PF00917	0.509
LIG_TRAF2_1	310	313	PF00917	0.643
LIG_TRAF2_1	351	354	PF00917	0.518
LIG_TYR_ITIM	116	121	PF00017	0.455
LIG_UBA3_1	33	38	PF00899	0.413
LIG_WW_3	229	233	PF00397	0.571
MOD_CK1_1	11	17	PF00069	0.583
MOD_CK1_1	130	136	PF00069	0.707
MOD_CK1_1	147	153	PF00069	0.495
MOD_CK1_1	219	225	PF00069	0.648
MOD_CK1_1	269	275	PF00069	0.450
MOD_CK1_1	325	331	PF00069	0.748
MOD_CK1_1	58	64	PF00069	0.633
MOD_CK2_1	11	17	PF00069	0.524
MOD_CK2_1	222	228	PF00069	0.727
MOD_CK2_1	233	239	PF00069	0.497
MOD_CK2_1	29	35	PF00069	0.351
MOD_CK2_1	347	353	PF00069	0.668
MOD_CK2_1	58	64	PF00069	0.606
MOD_GlcNHglycan	129	132	PF01048	0.666
MOD_GlcNHglycan	216	219	PF01048	0.424
MOD_GlcNHglycan	224	227	PF01048	0.807
MOD_GlcNHglycan	326	330	PF01048	0.651
MOD_GlcNHglycan	350	353	PF01048	0.623
MOD_GlcNHglycan	361	364	PF01048	0.548
MOD_GlcNHglycan	373	377	PF01048	0.411
MOD_GlcNHglycan	60	63	PF01048	0.721
MOD_GSK3_1	123	130	PF00069	0.517
MOD_GSK3_1	169	176	PF00069	0.579
MOD_GSK3_1	214	221	PF00069	0.281
MOD_GSK3_1	269	276	PF00069	0.470
MOD_GSK3_1	343	350	PF00069	0.652
MOD_GSK3_1	63	70	PF00069	0.731
MOD_GSK3_1	72	79	PF00069	0.749
MOD_GSK3_1	8	15	PF00069	0.557
MOD_N-GLC_1	269	274	PF02516	0.436
MOD_N-GLC_1	302	307	PF02516	0.602
MOD_NEK2_1	266	271	PF00069	0.370
MOD_NEK2_2	365	370	PF00069	0.501
MOD_PIKK_1	100	106	PF00454	0.507
MOD_PIKK_1	135	141	PF00454	0.619
MOD_PIKK_1	197	203	PF00454	0.360
MOD_PIKK_1	313	319	PF00454	0.701
MOD_PIKK_1	328	334	PF00454	0.486
MOD_PKA_1	347	353	PF00069	0.523
MOD_PKA_2	110	116	PF00069	0.417
MOD_PKA_2	347	353	PF00069	0.523
MOD_PKA_2	56	62	PF00069	0.605
MOD_Plk_1	207	213	PF00069	0.300
MOD_Plk_1	273	279	PF00069	0.489
MOD_Plk_1	365	371	PF00069	0.476
MOD_Plk_1	372	378	PF00069	0.363
MOD_Plk_1	63	69	PF00069	0.614
MOD_Plk_4	130	136	PF00069	0.588
MOD_Plk_4	278	284	PF00069	0.384
MOD_Plk_4	29	35	PF00069	0.410
MOD_Plk_4	294	300	PF00069	0.681
MOD_Plk_4	49	55	PF00069	0.549
MOD_Plk_4	8	14	PF00069	0.476
MOD_ProDKin_1	12	18	PF00069	0.506
MOD_ProDKin_1	123	129	PF00069	0.445
MOD_ProDKin_1	216	222	PF00069	0.593
MOD_ProDKin_1	336	342	PF00069	0.775
MOD_ProDKin_1	39	45	PF00069	0.575
MOD_ProDKin_1	77	83	PF00069	0.767
MOD_ProDKin_1	93	99	PF00069	0.416
TRG_DiLeu_BaEn_1	17	22	PF01217	0.418
TRG_DiLeu_BaEn_2	34	40	PF01217	0.422
TRG_DiLeu_BaLyEn_6	250	255	PF01217	0.392
TRG_ENDOCYTIC_2	118	121	PF00928	0.453
TRG_ENDOCYTIC_2	18	21	PF00928	0.366
TRG_ENDOCYTIC_2	9	12	PF00928	0.508
TRG_ER_diArg_1	109	112	PF00400	0.488
TRG_ER_diArg_1	85	88	PF00400	0.682
TRG_NLS_MonoExtC_3	107	112	PF00514	0.437
TRG_Pf-PMV_PEXEL_1	181	186	PF00026	0.363
TRG_Pf-PMV_PEXEL_1	190	194	PF00026	0.406

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1HTD1	Leptomonas seymouri	59%	100%
A0A3Q8ICC5	Leishmania donovani	94%	100%
A4HF63	Leishmania braziliensis	70%	99%
A4I2E6	Leishmania infantum	94%	100%
C9ZX62	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	29%	100%
E9AYJ9	Leishmania mexicana (strain MHOM/GT/2001/U1103)	90%	100%