Putative nitrilase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Putative nitrilase

Gene product:

nitrilase, putative

Species:

Leishmania major

UniProt:

Q4Q8W4_LEIMA

TriTrypDb:

LmjF.26.2280 , LMJLV39_260029400 , LMJSD75_260027800

Length:

279

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 3
Silverman et al.	yes	yes: 3
Pissara et al.	yes	yes: 15

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	yes	yes: 5

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 1, no: 10
NetGPI	no	yes: 0, no: 11

Could not find GO cellular_component term for this entry.

Expansion

Sequence features

Q4Q8W4

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4Q8W4

Function

Biological processes
Term	Name	Level	Count
GO:0006082	organic acid metabolic process	3	2
GO:0006107	oxaloacetate metabolic process	7	2
GO:0006520	amino acid metabolic process	3	2
GO:0006528	asparagine metabolic process	6	2
GO:0006541	glutamine metabolic process	6	2
GO:0006807	nitrogen compound metabolic process	2	12
GO:0008152	metabolic process	1	12
GO:0009064	glutamine family amino acid metabolic process	5	2
GO:0009066	aspartate family amino acid metabolic process	5	2
GO:0009987	cellular process	1	2
GO:0019752	carboxylic acid metabolic process	5	2
GO:0043436	oxoacid metabolic process	4	2
GO:0043648	dicarboxylic acid metabolic process	6	2
GO:0044237	cellular metabolic process	2	2
GO:0044238	primary metabolic process	2	2
GO:0044281	small molecule metabolic process	2	2
GO:0071704	organic substance metabolic process	2	2
GO:1901564	organonitrogen compound metabolic process	3	2
GO:1901605	alpha-amino acid metabolic process	4	2

Molecular functions
Term	Name	Level	Count
GO:0000257	nitrilase activity	5	7
GO:0003824	catalytic activity	1	12
GO:0016787	hydrolase activity	2	12
GO:0016810	hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds	3	12
GO:0016811	hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides	4	12
GO:0016815	hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in nitriles	4	7
GO:0050152	omega-amidase activity	5	2
GO:0080061	indole-3-acetonitrile nitrilase activity	6	7

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_NRD_NRD_1	114	116	PF00675	0.393
CLV_PCSK_KEX2_1	34	36	PF00082	0.441
CLV_PCSK_PC1ET2_1	34	36	PF00082	0.463
CLV_PCSK_SKI1_1	24	28	PF00082	0.565
DEG_APCC_DBOX_1	268	276	PF00400	0.553
DOC_MAPK_gen_1	34	41	PF00069	0.425
DOC_MAPK_MEF2A_6	151	158	PF00069	0.425
DOC_MAPK_RevD_3	263	279	PF00069	0.576
DOC_PP4_FxxP_1	46	49	PF00568	0.406
DOC_USP7_MATH_1	89	93	PF00917	0.482
DOC_USP7_UBL2_3	34	38	PF12436	0.526
DOC_WW_Pin1_4	208	213	PF00397	0.393
LIG_Actin_WH2_2	95	113	PF00022	0.491
LIG_BIR_II_1	1	5	PF00653	0.575
LIG_BRCT_BRCA1_1	100	104	PF00533	0.441
LIG_FHA_1	122	128	PF00498	0.437
LIG_FHA_1	172	178	PF00498	0.406
LIG_FHA_2	214	220	PF00498	0.491
LIG_LIR_Apic_2	44	49	PF02991	0.406
LIG_LIR_Gen_1	67	76	PF02991	0.397
LIG_LIR_Nem_3	159	165	PF02991	0.394
LIG_LIR_Nem_3	174	179	PF02991	0.387
LIG_LIR_Nem_3	52	58	PF02991	0.413
LIG_LYPXL_yS_3	162	165	PF13949	0.422
LIG_PTB_Apo_2	200	207	PF02174	0.422
LIG_PTB_Phospho_1	200	206	PF10480	0.526
LIG_SH2_PTP2	206	209	PF00017	0.526
LIG_SH2_STAP1	273	277	PF00017	0.534
LIG_SH2_STAP1	58	62	PF00017	0.422
LIG_SH2_STAT3	169	172	PF00017	0.491
LIG_SH2_STAT5	206	209	PF00017	0.526
LIG_SH2_STAT5	220	223	PF00017	0.406
LIG_SH2_STAT5	273	276	PF00017	0.620
LIG_SH2_STAT5	54	57	PF00017	0.422
LIG_SH3_3	183	189	PF00018	0.422
LIG_SH3_3	206	212	PF00018	0.319
LIG_SH3_3	225	231	PF00018	0.441
LIG_SUMO_SIM_anti_2	251	258	PF11976	0.514
LIG_SUMO_SIM_anti_2	37	44	PF11976	0.464
LIG_TYR_ITIM	160	165	PF00017	0.482
MOD_CDC14_SPxK_1	211	214	PF00782	0.406
MOD_CDK_SPxK_1	208	214	PF00069	0.406
MOD_CK1_1	100	106	PF00069	0.526
MOD_CK2_1	142	148	PF00069	0.526
MOD_CK2_1	213	219	PF00069	0.463
MOD_CK2_1	84	90	PF00069	0.406
MOD_GlcNHglycan	135	138	PF01048	0.459
MOD_GlcNHglycan	215	219	PF01048	0.491
MOD_GlcNHglycan	90	94	PF01048	0.552
MOD_GSK3_1	129	136	PF00069	0.354
MOD_N-GLC_1	65	70	PF02516	0.526
MOD_N-GLC_1	98	103	PF02516	0.441
MOD_NEK2_1	97	102	PF00069	0.397
MOD_PIKK_1	70	76	PF00454	0.541
MOD_PK_1	77	83	PF00069	0.463
MOD_PKA_1	34	40	PF00069	0.463
MOD_PKA_2	213	219	PF00069	0.463
MOD_PKA_2	34	40	PF00069	0.463
MOD_Plk_1	139	145	PF00069	0.570
MOD_Plk_1	250	256	PF00069	0.491
MOD_Plk_1	65	71	PF00069	0.486
MOD_Plk_1	98	104	PF00069	0.441
MOD_Plk_4	172	178	PF00069	0.406
MOD_Plk_4	223	229	PF00069	0.411
MOD_Plk_4	24	30	PF00069	0.514
MOD_Plk_4	250	256	PF00069	0.486
MOD_Plk_4	77	83	PF00069	0.490
MOD_ProDKin_1	208	214	PF00069	0.393
MOD_SUMO_rev_2	241	250	PF00179	0.553
MOD_SUMO_rev_2	90	98	PF00179	0.431
TRG_DiLeu_BaEn_2	240	246	PF01217	0.526
TRG_ENDOCYTIC_2	162	165	PF00928	0.434
TRG_ENDOCYTIC_2	206	209	PF00928	0.526
TRG_ER_diArg_1	259	262	PF00400	0.613

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1I1H1	Leptomonas seymouri	79%	100%
A0A0S4ILR2	Bodo saltans	56%	98%
A0A140NCB4	Escherichia coli (strain B / BL21-DE3)	25%	100%
A0A140NDS5	Escherichia coli (strain B / BL21-DE3)	23%	100%
A0A1X0P8A8	Trypanosomatidae	73%	100%
A0A3S7X065	Leishmania donovani	97%	100%
A0A422P3W9	Trypanosoma rangeli	66%	100%
A4HF72	Leishmania braziliensis	89%	100%
A4I2F5	Leishmania infantum	97%	100%
C9ZX42	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	66%	100%
E9AYK8	Leishmania mexicana (strain MHOM/GT/2001/U1103)	96%	100%
K2QXC4	Macrophomina phaseolina (strain MS6)	24%	81%
O31664	Bacillus subtilis (strain 168)	31%	100%
O59829	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	29%	100%
O94660	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	31%	100%
P0DP65	Staphylococcus aureus (strain 04-02981)	30%	100%
P0DP66	Synechocystis sp. (strain PCC 6803 / GT-S)	30%	100%
P0DP67	Yersinia enterocolitica	23%	100%
P0DP68	Yersinia enterocolitica	27%	98%
P32962	Arabidopsis thaliana	24%	82%
P47016	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	26%	91%
P49954	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)	43%	96%
P55175	Synechocystis sp. (strain PCC 6803 / Kazusa)	30%	100%
P55176	Pseudomonas protegens (strain DSM 19095 / LMG 27888 / CFBP 6595 / CHA0)	27%	98%
P55177	Staphylococcus aureus	29%	100%
P55178	Staphylococcus lugdunensis	27%	100%
P58054	Escherichia coli O157:H7	25%	100%
P9WJ00	Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)	25%	100%
P9WJ01	Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)	25%	100%
Q10166	Schizosaccharomyces pombe (strain 972 / ATCC 24843)	48%	87%
Q28IE5	Xenopus tropicalis	47%	100%
Q2T9R6	Bos taurus	47%	100%
Q32LH4	Bos taurus	33%	85%
Q3HVN1	Solanum tuberosum	22%	93%
Q47679	Escherichia coli (strain K12)	23%	100%
Q497B0	Rattus norvegicus	48%	100%
Q4VBV9	Danio rerio	49%	100%
Q54JM9	Dictyostelium discoideum	46%	85%
Q557J5	Dictyostelium discoideum	30%	96%
Q5NHL7	Francisella tularensis subsp. tularensis (strain SCHU S4 / Schu 4)	24%	98%
Q5R4L6	Pongo abelii	46%	100%
Q6INI7	Xenopus laevis	48%	100%
Q6IR61	Xenopus laevis	47%	100%
Q75SP7	Pseudomonas sp.	26%	100%
Q7TQ94	Rattus norvegicus	33%	85%
Q86X76	Homo sapiens	31%	85%
Q8RUF8	Arabidopsis thaliana	44%	76%
Q8VDK1	Mus musculus	34%	86%
Q8VYF5	Arabidopsis thaliana	23%	93%
Q93NG1	Paenarthrobacter nicotinovorans	28%	95%
Q93XI4	Oryza sativa subsp. japonica	24%	93%
Q94JV5	Arabidopsis thaliana	27%	91%
Q9JHW2	Mus musculus	47%	100%
Q9NQR4	Homo sapiens	46%	100%
Q9UYV8	Pyrococcus abyssi (strain GE5 / Orsay)	29%	100%
Q9XGI9	Solanum lycopersicum	22%	93%
V5B8Z7	Trypanosoma cruzi	63%	100%