Putative metallopeptidase

Quick info Localization Expansion Sequence features Structure Function Putative motif mimicry Homologs Download

Quick info

Protein:

Gene product:

Lys-63-specific deubiquitinase BRCC36, putative

Species:

Leishmania major

UniProt:

Q4Q8U8_LEIMA

TriTrypDb:

LmjF.26.2430 , LMJLV39_260031000 , LMJSD75_260029400

Length:

336

Localization

Secreted promastigote


Source	Evidence on protein	Close homologs
Cuervo et al.	no	yes: 0
Hassani et al.	no	yes: 0
Forrest at al. (metacyclic)	no	yes: 0
Forrest at al. (procyclic)	no	yes: 0
Silverman et al.	no	yes: 0
Pissara et al.	no	yes: 0

Secreted amastigote


Source	Evidence on protein	Close homologs
Pires et al.	no	yes: 0

Exosome


Source	Evidence on protein	Close homologs
Silverman et al.	no	yes: 0

Glycosome


Source	Evidence on protein	Close homologs
Jamdhade et al.	no	yes: 0

Predictions


Source	Evidence on protein	Close homologs
DeepLoc
SignalP6	no	yes: 0, no: 7
NetGPI	no	yes: 0, no: 7

Cellular components
Term	Name	Level	Count
GO:0032991	protein-containing complex	1	3
GO:0070531	BRCA1-A complex	3	3
GO:0070552	BRISC complex	3	3
GO:0140513	nuclear protein-containing complex	2	3

Expansion

Sequence features

Q4Q8U8

Sequence

MSA

Disorder

Secondary

Topology

Domains

SignalP

GPI

Phosphorylations

ELMs

Structure

Predicted structure by AlphaFold2

Related structures:

AlphaFold database: Q4Q8U8

Function

Biological processes
Term	Name	Level	Count
GO:0006139	nucleobase-containing compound metabolic process	3	3
GO:0006259	DNA metabolic process	4	3
GO:0006281	DNA repair	5	3
GO:0006302	double-strand break repair	6	2
GO:0006508	proteolysis	4	3
GO:0006725	cellular aromatic compound metabolic process	3	3
GO:0006807	nitrogen compound metabolic process	2	3
GO:0006950	response to stress	2	3
GO:0006974	DNA damage response	4	3
GO:0008152	metabolic process	1	3
GO:0009987	cellular process	1	3
GO:0016570	histone modification	5	2
GO:0016578	histone deubiquitination	6	2
GO:0016579	protein deubiquitination	6	3
GO:0019538	protein metabolic process	3	3
GO:0033554	cellular response to stress	3	3
GO:0034641	cellular nitrogen compound metabolic process	3	3
GO:0036211	protein modification process	4	3
GO:0043170	macromolecule metabolic process	3	3
GO:0043412	macromolecule modification	4	3
GO:0044237	cellular metabolic process	2	3
GO:0044238	primary metabolic process	2	3
GO:0044260	obsolete cellular macromolecule metabolic process	3	3
GO:0046483	heterocycle metabolic process	3	3
GO:0050896	response to stimulus	1	3
GO:0051716	cellular response to stimulus	2	3
GO:0070536	protein K63-linked deubiquitination	7	3
GO:0070537	histone H2A K63-linked deubiquitination	7	2
GO:0070646	protein modification by small protein removal	5	3
GO:0070647	protein modification by small protein conjugation or removal	5	3
GO:0071704	organic substance metabolic process	2	3
GO:0090304	nucleic acid metabolic process	4	3
GO:1901360	organic cyclic compound metabolic process	3	3
GO:1901564	organonitrogen compound metabolic process	3	3

Molecular functions
Term	Name	Level	Count
GO:0003824	catalytic activity	1	8
GO:0005488	binding	1	2
GO:0005515	protein binding	2	2
GO:0008233	peptidase activity	3	8
GO:0008237	metallopeptidase activity	4	8
GO:0016787	hydrolase activity	2	8
GO:0031593	polyubiquitin modification-dependent protein binding	4	2
GO:0140030	modification-dependent protein binding	3	2
GO:0140096	catalytic activity, acting on a protein	2	8
GO:0004843	cysteine-type deubiquitinase activity	5	1
GO:0008234	cysteine-type peptidase activity	4	1
GO:0019783	ubiquitin-like protein peptidase activity	4	1
GO:0101005	deubiquitinase activity	5	1

Putative motif mimicry


Leishmania	From	To	Domain/Motif	Score
CLV_C14_Caspase3-7	260	264	PF00656	0.541
CLV_NRD_NRD_1	301	303	PF00675	0.560
CLV_PCSK_KEX2_1	108	110	PF00082	0.504
CLV_PCSK_PC1ET2_1	108	110	PF00082	0.516
CLV_PCSK_SKI1_1	35	39	PF00082	0.464
DEG_APCC_DBOX_1	16	24	PF00400	0.523
DEG_Nend_UBRbox_3	1	3	PF02207	0.699
DOC_MAPK_DCC_7	167	177	PF00069	0.285
DOC_MAPK_gen_1	280	290	PF00069	0.513
DOC_USP7_MATH_1	276	280	PF00917	0.574
DOC_USP7_MATH_1	7	11	PF00917	0.599
DOC_WW_Pin1_4	170	175	PF00397	0.288
DOC_WW_Pin1_4	247	252	PF00397	0.474
DOC_WW_Pin1_4	71	76	PF00397	0.623
LIG_14-3-3_CanoR_1	147	154	PF00244	0.401
LIG_14-3-3_CanoR_1	24	30	PF00244	0.455
LIG_14-3-3_CanoR_1	35	45	PF00244	0.459
LIG_Actin_WH2_2	22	37	PF00022	0.432
LIG_APCC_ABBA_1	55	60	PF00400	0.439
LIG_BRCT_BRCA1_1	233	237	PF00533	0.564
LIG_Clathr_ClatBox_1	290	294	PF01394	0.552
LIG_EH1_1	290	298	PF00400	0.449
LIG_FHA_1	154	160	PF00498	0.346
LIG_FHA_1	248	254	PF00498	0.428
LIG_FHA_2	266	272	PF00498	0.488
LIG_FHA_2	299	305	PF00498	0.560
LIG_FHA_2	37	43	PF00498	0.366
LIG_LIR_Gen_1	234	245	PF02991	0.581
LIG_LIR_Nem_3	188	194	PF02991	0.343
LIG_LIR_Nem_3	234	240	PF02991	0.543
LIG_RPA_C_Fungi	314	326	PF08784	0.395
LIG_SH2_STAT5	114	117	PF00017	0.459
LIG_SH2_STAT5	157	160	PF00017	0.425
LIG_SH3_3	163	169	PF00018	0.401
LIG_SH3_3	221	227	PF00018	0.557
LIG_SH3_3	294	300	PF00018	0.489
LIG_SH3_3	69	75	PF00018	0.585
LIG_SUMO_SIM_par_1	173	179	PF11976	0.390
LIG_TRAF2_1	39	42	PF00917	0.385
MOD_CDK_SPK_2	247	252	PF00069	0.471
MOD_CK1_1	134	140	PF00069	0.396
MOD_CK1_1	85	91	PF00069	0.576
MOD_CK2_1	137	143	PF00069	0.347
MOD_CK2_1	265	271	PF00069	0.486
MOD_CK2_1	298	304	PF00069	0.532
MOD_CK2_1	36	42	PF00069	0.357
MOD_GlcNHglycan	102	105	PF01048	0.664
MOD_GlcNHglycan	132	136	PF01048	0.552
MOD_GlcNHglycan	139	142	PF01048	0.328
MOD_GlcNHglycan	188	191	PF01048	0.381
MOD_GlcNHglycan	233	236	PF01048	0.474
MOD_GlcNHglycan	271	274	PF01048	0.546
MOD_GlcNHglycan	87	90	PF01048	0.730
MOD_GlcNHglycan	9	12	PF01048	0.627
MOD_GSK3_1	149	156	PF00069	0.399
MOD_GSK3_1	194	201	PF00069	0.373
MOD_GSK3_1	265	272	PF00069	0.567
MOD_N-GLC_1	203	208	PF02516	0.561
MOD_N-GLC_1	7	12	PF02516	0.612
MOD_NEK2_1	102	107	PF00069	0.688
MOD_NEK2_1	194	199	PF00069	0.335
MOD_NEK2_1	324	329	PF00069	0.602
MOD_PIKK_1	176	182	PF00454	0.312
MOD_PKA_2	13	19	PF00069	0.610
MOD_PKA_2	146	152	PF00069	0.328
MOD_PKA_2	23	29	PF00069	0.491
MOD_PKA_2	269	275	PF00069	0.588
MOD_PKA_2	85	91	PF00069	0.590
MOD_Plk_4	194	200	PF00069	0.435
MOD_ProDKin_1	170	176	PF00069	0.288
MOD_ProDKin_1	247	253	PF00069	0.466
MOD_ProDKin_1	71	77	PF00069	0.625
MOD_SUMO_for_1	107	110	PF00179	0.600
TRG_DiLeu_BaEn_1	44	49	PF01217	0.406
TRG_DiLeu_BaLyEn_6	249	254	PF01217	0.427
TRG_DiLeu_BaLyEn_6	308	313	PF01217	0.517
TRG_ER_diArg_1	83	86	PF00400	0.591
TRG_Pf-PMV_PEXEL_1	283	287	PF00026	0.418

Homologs


Protein	Taxonomy	Sequence identity	Coverage
A0A0N1PFL1	Leptomonas seymouri	53%	100%
A0A1X0P845	Trypanosomatidae	37%	100%
A4HF88	Leishmania braziliensis	75%	100%
B2RYM5	Rattus norvegicus	33%	100%
C9ZX24	Trypanosoma brucei gambiense (strain MHOM/CI/86/DAL972)	40%	100%
E9AYM4	Leishmania mexicana (strain MHOM/GT/2001/U1103)	89%	100%
P46737	Mus musculus	33%	100%
V5BGS3	Trypanosoma cruzi	42%	100%